Biosynthesis of

Biosynthesis ofBiosynthesis of

生科系博士班 884234 李曉青資工系博士班 914338 張君天資工系博士班 928307 林沿妊

Group 3

Powerful antioxidant, reduces the risk of

– Coronary heart disease by preventing oxidation of low density lipoprotein (LDL) cholesterol

– Atherosclerosis by protecting plasma lipids from oxidation

– Oxidative damage of lymphocyte DNA

– Cancer

Protein ID

Protein name Gene

2.2.1.7 1-deoxy-D-xylulose-5-phosphate synthase dxs E.coli1.1.126

72-C-methyl-D-erythritol 4-phosphate (MEP) synthase dxr E.coli

2.7.7.602-C-methyl-D-erythritol 4-phosphate cytidylyltransf

erase ygbP E.coli

2.7.1.148

4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase ychB E.coli

4.6.1.122-C-methyl-D-erythritol 2,4-cyclodiphosphate synth

ase ygbB E.coli

1.17.4.3 

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase gcpE E.coli

1.17.1.2 4-hydroxy-3-methylbut-2-enyl diphosphate reducta

se lytB E.coli

5.3.3.2 isopentenyl-diphosphate delta-isomerase b2889

E.coli

2.5.1.10 farnesyl-diphosphate synthase ispA E.coli

2.5.1.29 geranylgeranyl pyrophosphate synthetase crtE E.uredovora

2.5.1.32 phytoene synthetase crtB E.uredovora

1.3.99- oxidoreductase crtI E.uredovora

1.14.99.30

zeta-carotene desaturase crtQ E.uredovora

dxs:DXP synthaseidi: IPP isomeraseispA: Farnesyl-PP synthase

crt E: GGPP synthasecrt B: phytoene synthasecrt I: lycopene synthase from E.uredovora gps:ggpp synthase

from A.fulgidus

Red : E.coli

Blue : A.fulgidus

Green: E.uredovora

Wang CW, Oh MK, Liao JC. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli.Biotechnol Bioeng. 1999 Jan 20;62(2):235-41.

Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K, Harashima K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli.J Bacteriol. 1990 Dec;172(12):6704-12.

Farmer WR, Liao JC. Improving lycopene production in Escherichia coli by engineering metabolic control.Nat Biotechnol. 2000 May;18(5):533-7.

References