Domain Bakteria

Domain Bakteria

• Dunia bakteria: 23 Phylum

Bakteria: koloni

Sel Bakteri: morfologi

Sela Bakteria : morfologi

Borellia sp.

Sianobakteria

Salmonella sp.Bacillus antharcis

Bakteri: Actinomycetes

Sel bakteri: Actinomycetes

Streptomyces indonesiensis

Sel Bakteria: Tanpa organela

Struktur sel bakteri

Struktur sel bakteria

Struktur sel bakteria• Tidak ada organela• Kromosom tunggal (DNA sirkular) dalam

sitoplasma

Filogeni Archaea

Archaeoglobus Methanococcus vannielii

Desulfurococcus

Sulfolobus

pJP 9685/74pSL 12

pSL17pSL22pSL4Marine SBAR5

pJP 27pJP 78

Thermoplasma

MethanospirillumHaloverax

Methanococcus jannaschiiThermococcus

Methanopyrus

BACTERIA

EUKARYOTES

Kararchaeota

pSL 50

ThermoproteusThermofilum

Crenarchaeota

Euryarchaeota

Methanothermus

Methanobacterium

Pyrodictium

Filogeni bakteria

0.1 changes per nucleotide (nt)

Spirochetes

Green Sulfur Bacteria

Bacteroides and relatives

Chlamydiae

Planctomyces

CyanobacteriaGram positive

Aquificoles

Proteobacteria

Green Nonsulfur Bacteria

Thermotogales

Deinococci

ARCHAEAAnd

EUKARYA

BACTERIA

Bakteria: 23 Phylum1 Aquificae2.Thermotogae3. Thermodesulfobacteria4. Deinococcus-Thermus5. Chrysiogenetes6. Chloroflexi7. Thermomicrobia8. Nitrospira

Bakteria: 23 Phylum

• 9. Deferribacteres 17. Spirochaetes• 10. Cyanobacteria 18. Fibrobacteres• 11. Chlorobi 19. Acidobacteria• 12. Proteobacteria 20. Bacteroidetes• 13. Firmicutes 21. Fusobacteria• 14. Actinobacteria 22. Verrucobacteria• 15.Planctomycetes 23. Dictyoglomi• 16. Chlamydae

Phylum Actinobacteria

• Classis: Actinobacteria (5 subclassis)• 1. Acidimicrobidae• 2. Rubrobacteridae• 3. Coriobacteridae• 4. Sphaerobacteridae• 5. Actinobacteridae (2 Ordo)

Subclassis Actinobacteridae1. Ordo Actinomycetales ( 10 Sub-ordo):• 1.1. Actinomycinea ( 1 Familia; 4 genera)• 1.2. Micrococcineae (10 Familia)• 1.3. Corynebacterineae (7 Familia)• 1.4. Micromonosporineae (1 familia; 9 genera)• 1.5. Propionibacterineae (2 Familia)• 1.6. Pseudonocardineae (2 Familia)• 1.7. Streptomycineae (1 Familia; 3 genera)• 1.8. Streptosporangineae (3 Familia)• 1.9. Frankineae (6 Familia0• 1.10. Glycomycineae (1 Familia; 1 genus)

2. Ordo Bifidobacterales

Sub-ordo: Streptomycineae

• Familia:Streptomycetaceae• Genus: Streptomyces• Anggota spesies: sekitar 500Krakterisitik Genus Streptomyces:• Memiliki hifae vegetatif dan hifa aerial yang

menghasilkan rantai konidiospora yang terdiri dari 3 - 50

Paraserianthes falcatharia

Homo sapiens

Selective isolation of Streptomycetes from the rhizosphere of P. falcatharia.

Koloni pada Strach-casein

Koloni pada Raffinose-histidin

Koloni kultur murni

Rantai dan ornamen spora

Streptomyces indonesiensis DSM 41759T

Kode Strain (Acession Number)

• S. asiaticus (A14P1 = DSM 41761 = NCIMB 13675)

• S. cangkringensis (D13P3 = DSM 41769 = NCIMB 13684)

• S. indonesiensis (A4R2 = DSM 41759 = NCIMB 13673)

• (Dapat dilihat di Website DSMZ CC)

Kode Strain (Acession Number)

• S. javensis (B22P3 = DSM 41764 = NCIMB 13679)

• S. rhizosphaerius (A10P1 = DSM 41760 = NCIMB 13674)

• S. yogyakartensis (C4R3 = DSM 41766 = NCIMB 13681)

Acession Number 16S rDNA

• S. asiaticus DSM 41761T (AJ 391830) • S. cangkringensis DSM 41769T (AJ 391831)• S. indonesiensis DSM 41759 T(AJ 391835) • S. javensis DSM 41764T (AJ 391833)• S. rhizosphaerius DSM 41760T (AJ 391834)• S. yogyakartensis DSM 41766T (AJ 391827)

Publikasi

• Draf publikasi • Kode Strain dari DSMZ dan NCIMB• Accession number of 16S rDNA seq.

↓Int. J. General & Mol. Microbiol. Antonie

van Leeuwenhoek↓

Vol. 78 (3/4): 353 – 366 (Effective)

Validation

Valid Publication:• International Journal of Systematic and

evolutionary Microbiology 51: 1619-1620 (2001)

↓Approved List of Bacterial Name

Bahan bioaktif: Antitumor

Antitumor Producer

Daunorubicin Streptomyces peuceticus

Actinomycin S. antibioticus

Mitomycin S. caespitosus

Bleomycin S. verticillus

Rapamycin S. indonesiensis-group

Bahan bioaktif: Antibiotic Antibiotic Producer

Erythromycin Streptomyces erythreus

Chloramphenicol S. venezuellae

Rifampicin S. mediterranei

Novobiocin S. spheroides

Streptomycin S. griseus

Tetracyclin S. aureofciens

Bahan bioaktif: Antifungal Antifungal Producer

Cycloheximide Streptomyces griseus

Nystatin S. noursei

Griseofulvin S. griseofulvum

Amphotericin B S. nodosus

Antihelminth Producer

Ivermectin S. avermitoles

Life cycle

Morfology hifa

Koloni

Morfologi rantai spora

SEM of spore chain

Pigmen streptomyces

Research Poster

Research Poster

Genus Aquifex

Aquifex pyrophylusAquifex aerolicus

Genus Thermotoga

Habitat Thermotoga

Thermotoga maritima

3. Phylum: Thermodesulfobacteria • Thermodesulfobacterium • Thermodesulfatator

Thermodesulfobacterium hydrogeniphilim

4. Phylum Deinococcus-Thermus

• Deinococcus • Thermus • Marinithermus • Meiothermus • Oceanithermus • Vulcanithermus

Thermus aquaticus

6. Phylum Chloroflexi (green nonsulfur bacteria)

• Chloroflexus • Chloronema • Heliothrix • Roseiflexus • Oscillochloris • Herpetosiphon • Anaerolinea • Caldilinea

Chloroflexus

7. Phylum Thermomicrobia• Thermomicrobium roseum • Thermomicrobium fosteri

Thermomicroboim sp.

8. Phylum Nitrospira

• Nitrospira • Leptospirillum • Magnetobacterium • Thermodesulfovibrio

Leptospirillum ferrooxidans

Habitat

Cyanobacteria

Spirulina sp.Oscilatoria sp.

Nostoc sp.Anabaena sp.

Bacillus thuringiensis

Toksisitas Bt

Mekanisme kerja toksin BT

17. Phylum Spirochaetes• Spirochaetes • Borrelia • Brachyspira • Brevinema • Clevelandina • Cristispira • Diplocalyx • Hollandina • Leptonema • Leptospira • Pillotina • Serpulina • Spirochaeta • Treponema

Leptospira

20. Phylum Bacteroidetes

• Bacteroides: Bacteroides fragilis

21. Phylum Fusobacteria

Fusobacterium nucleatum

22. Phylum Verrucomicrobia

• Opitutus • Prosthecobacter • Verrucomicrobium • Victivallis • Xiphinematobacter

Prosthecobacter fusiformis