View
218
Download
0
Category
Preview:
Citation preview
Oxidative Phosphorylation
Part 2
Chapter 19
Oxidative Phosphorylation Part 2
Key Topics: To Know
1.How cells deal with reactive oxygen species (ROS).2.Calculating ΔGo’ of the Proton Motive Force.3.Membrane ATPase and how it works.4.Cytoplasmic NADH getting into the mitochondria.5. Adenylate Control.6. Mitochondia and apoptosis.7.PMF can be used to ?
ROS : Reactive Oxygen Species
ΔG of the Proton Motive Force
Nobel Prize for Chemiosmotic Model = PMF
Converting PMF to ATP
Mitochondrial Respiration+Phosphorylation
EOC Problem 9: Compartmentalization of Citric Acid Cycle.
Adjusting the pH Generates ATP
Molecules that Collapse the PMF
DNP is an Uncoupler
EOC Problem 6: Figuring out what happens with uncouplers.
Odorous Flowers that Heat Up Using Uncoupled Respiration
Worked out F1 ATPase kinetic mechanism + First purified the FoF1 ATPase
ATP Synthase
ATP Synthase Catalyzes by Steps
F1 Fo ATP synthase
F1 Complex
Used to Inhibit ATP Synthase Reaction
F1 ATPase Spinning
ATP Synthase Seen Rotating !
ATP Synthase Substrate Transport
ADP and Pi are products of Anabolism
EOC Problem 11 is the
effect of ADP and Pi on ATP
Synthesis.
Get Ready, NADH formed in Cytoplasm
in Next
Glycolysis NADH Enters the Mitochondria by the Malate Aspartate Shuttle
NADH from Glycolysis Can Also Get in by Glycerol Phosphate DH
Also has a Anabolic Role
EOC Problems 13 and 14: Get in to the Pasteur Effect and “Petites” in Yeast Colonies. Fun stuff.
Adenylate Control of Glycolysis/CAC/e-transport
1. Acceptor Control: availability of ADP + Pi
2. Mass action ratio: [ATP]/([ADP] [Pi])
EOC Problem 17 Gets into the rate of ATP turnover in heart muscle…the muscle that always has to be “on”.
EOC Problem 18 Gets into the same in insect flight muscle.
Inhibitory Protein IF1 Stops Loss of ATP During Ischemia that is Hypoxia
IF (red and white) forms dimers at pH 6.5 to Stop Rotation of ATP Synthase
Thermogenin – Protein Uncoupler in Brown Fat
Mitochondria in Adrenal Gland: P-450 Oxygenases Specialized for Steroid Synthesis
Electron Flow to P-450
Apoptosis is Regulated by the
Mitochondria
Initiates a series of proteasess
Stress, Damage
EOC Problem 19: relates mitochondrial function to cancer. Hint think about the first couple of slides.
Escherichia coli Electron Transport
Bacterial Quinone
Bacteria Use the PMF to Rotate Their Flagella
Proton Motive Force Functions To
1. Synthesize ATP (from ADP + Pi).
2. Active Transport (Symports, Antiports, Uniports; review Chapter 11).
3. Rotate Bacterial Flagella.
4. Reversed Electron Transport (some Photosynthetic and Chemoautotrophic Bacteria)
Things to Know and Do Before Class1. How cells deal with reactive oxygen
species (ROS).
2. Calculating ΔGo’ of the Proton Motive Force.
3. Membrane ATPase and how it works.
4. Cytoplasmic NADH getting into the mitochondria.
5. Adenylate Control.
6. Mitochondria and apoptosis.
7. PMF can be used to ?
8. EOC Problems: 6, 9, 11,13,14, 17, 18, 19.
Recommended