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SEPT9 Polyclonal ANTIBODYCatalog Number:10769-1-AP Featured Product 12 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:10769-1-AP
Size:150UL , Concentration: 240 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG1218
GenBank Accession Number:BC021192
GeneID (NCBI):10801
Full Name:septin 9
Calculated MW:64 kDa
Observed MW:65 kDa, 38-40 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:500-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:2000for WBIHC 1:50-1:500 IF 1:20-1:200
Applications Tested Applications:FC, IF, IHC, IP, WB, ELISA
Cited Applications:IF, IHC, WB
Species Specificity:human, mouse, rat
Cited Species:human, mouse, rat
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : mouse thymus tissue, C6 cells, HeLa cells, mousebrain tissue, mouse spleen tissue
IP : mouse brain tissue,
IHC : human pancreas cancer tissue, human testistissue
IF : HepG2 cells, hTERT-RPE cells
Background Information SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all ofthe isoforms about SEPT9.
Notable Publications Author Pubmed ID Journal Application
Sellin Mikael E ME 21737677 Mol Biol Cell WB
Julia Pfanzelter 29921601 J Cell Biol IF
Katharina Neubauer 30019536 Cytoskeleton (Hoboken) IF
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: proteintech@ptglab.com W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
IF result of anti-SEPT9 (10769-1-AP, 1:200) withhTERT-RPE cell by Moshe Kim (laboratory of Dr.William S Trimble, University of Toronto). Green:SEPT9. Blue: DNA. Fixation in methanol.
mouse thymus tissue were subjected to SDS PAGEfollowed by western blot with 10769-1-AP (SEPT9antibody) at dilution of 1:600 incubated at roomtemperature for 1.5 hours.
Immunofluorescent analysis of HepG2 cells, usingSEPT9 antibody 10769-1-AP at 1:50 dilution andRhodamine-labeled goat anti-rabbit IgG (red). Bluepseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slideusing 10769-1-AP (SEPT9 antibody) at dilution of1:200 (under 10x lens. Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slideusing 10769-1-AP (SEPT9 antibody) at dilution of1:200 (under 40x lens. Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).
IP Result of anti-SEPT9 (IP:10769-1-AP, 4ug;Detection:10769-1-AP 1:1000) with mouse braintissue lysate 4000ug.
1X10^6 HepG2 cells were stained with 0.2ug SEPT9antibody (10769-1-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3% BSA(30 min). Alexa Fluor 488-conjugated AffiniPureGoat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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