Microbial production of methanol (1)

1

Microbial Production of Methanol

Presented By: Mohsin ShadRegistration Number: 2013-BC-005Date: 18-04-2016Presented BY: Dr. Muhammad Imran

2

Methanol

Methanol, also known as Methyl Alcohol Methanol is the simplest alcohol (CH3OH) Methyl group linked to a Hydroxyl group Light, volatile, colorless, flammable liquid Methanol is highly toxic and unfit for

consumption.

3

Microbial Production of Methanol

Methanol is Produced By: (1) Yeast (2) Fungi (3) Bacteria Microbes produce Pectin Methyl Esterase PME Produce Methanol from Fruits And Juices Microbes can also produce methanol via the

oxidation of methane (biogas)

4

Pectin

Structural Hetero-polysaccharide Gelling agent in food Source of dietary fiber Stabilizer in fruit juices and milk drinks Methanol is produced by fermentation of fruits Pectin Methyl Esterase (PME) PME produced by Microorganism

5

Production of Methanol by Pectin

Pectin enzymes are produced by yeast, bacteria, fungi and plants

Proteolytic enzymes are classified into: (1) Esterases (2) Depolymerase (lyases and hydrolyses) lyases produces oligo- or mono-galacturonate Hydrolysis of pectin by esterases produces pectic

acid and methanol

6

Production of Methanol by Pectin

PME hydrolyses pectin to poly-galactouronic acid and methanol

7

Methane Monooxygenase

PMMO – Particulate Methane Monooxygenase SMMO – Soluble Methane Monoxygenase

8

Advantages of using Ammonia-Oxidizing Bacteria

Oxidize methane to methanol via the nonspecific

action of the enzyme ammonia monooxygenase

Contaminants such as moisture and CO2 do not

post a limitation for biological conversion

Can utilize the CO2 contained in gas mixtures for

cell synthesis

9

Metabolism of AOB

10

Methanol Production by AOB

Methanol production rate varies for conditions and

performance

Maximum specific productivity is 0.82 mg

methanol/ mg biomass (COD)/d

11

Uses of Methanol

Methanol is used in: Fuel Antifreeze Cleaners Canned Heat Paints, Paint Thinner and Removers

12