Self Introduction

Educ

atio

n

Educ

atio

nBasic

Info

Basic In

fo

Employm

ent

Employm

ent

山東省中國

諸城市

諸城市

around 30 years ago

姓：宋 (Song, Soo)名：傑 (Jie, Ketsu)Jason Soung

恐龙化石Dinosaur Fossil鸭嘴龙 -hadrosaur

Village elementary school: 5 years

Shandong University: 4 years

1st County high school: 3 years

Town junior high school: 4 years

Biology Olympics Contest

To be a MasterTo be a Master

Company

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Isolation and Identification of the Strains Producing Alginase, Purification, Characterization and Enzymatic Degradation of the ALY-SJ02 ， Cloning and Expression of the Alginase Gene

Isolation and Identification of the Strains Producing Alginase, Purification, Characterization and Enzymatic Degradation of the ALY-SJ02 ， Cloning and Expression of the Alginase Gene

Supervisor: Prof. Xiulan Chen Prof. Yuzhong Zhang

Supervisor: Prof. Xiulan Chen Prof. Yuzhong Zhang

Background

Alginates are synthesized as cell wall components by brown seaweeds and as exo-polysaccharides by two families of heterotrophic bacteria, Pseudomonadaceae and Azotobacteriaceae.

Alginate is widely used as a stabilizer, viscosifier, and gelling agent in the food and beverage, cosmetics, paper and printing, biomaterials, and pharmaceutical industries.

Background Alginate is an acidic linear polysaccharide

composed of (1→4)-linked α-L-guluronate (G) and its C5 epimer β-D-mannuronate (M); the uronic acids are arranged in three types of block structures, homopolymeric polyguluronate (PG), homopolymeric polymannuronate (PM), and heteropolymeric sequences of randomly distributed the residues (PMG), which are named G-blocks, M-blocks, and MG-blocks, respectively.

Alginates can be degraded by alginate lyases (alginases) which usually catalyze the cleavage of the glycosidic linkages via a β-elimination mechanism. Polysaccharide lyases are classified into 18 families (PL-1 to -18). Alginate lyases mainly occupy two families (PL-5 and -7).

Isolation & identification of the Strain

Optimization of

fermentation condition

Purification & characterizati

on of Alginase

Cloning & expression of Alginase geneSmall-scale

process development for

industrial utilization

Structure

Analysis

Program Flowchart

Engineering bacteria constructi

on

Strain SM0524 & ALY-SJ02

Impact Factor: 3.98

0524

-20

0

20

40

60

80

100

120

Rel

ativ

e ac

tivity

(%)

-0.4

0.1

0.6

1.1

1.6

2.1

2.6

OD

520/

680

相对酶活无用生物量降解度

opti mum te

mperature

0

20

40

60

80

100

120

2030

4050

6070

80

temperatur

e ℃rela

tive

act

ivit

y ％y = 0. 3131x + 0. 096

R2 = 0. 998

0

0. 1

0. 2

0. 3

0. 4

0. 5

0. 6

0. 7

0. 8

0 1 2 31/ [S] ml / mg

1/v min/ug/ml

Km=3.2615mg/mlVmax=10.4167μg/ml/min

Heterogenous Expression

N-terminal sequence MALDI-TOF-MS

系统进化树→序列比对

TAIL-PCR

Expressed ALY-SJ 02

-20

0

20

40

60

80

100

120

20 30 40 50 60 70

Temperature(℃)Relative Activity(%)

Characterization of recombinant Alginase

Structure Analysis

-20

20

-10

0

10

190 250200 220 240

CD[mdeg]

Wavelength [nm]

Family PL-7

Process development Response Surface Method (RSM), 1951 年 , Box-Wilsong,

multiple factors, cross-interaction, limited time. Degrading Ratio ： CCD: 3/3/17

Optimized Condition:

pH: 6.7

Time: 289min

Temperature: 38.8℃

Optimized Degrading Ratio:

71.35%± 0.02153

GenScipt

Presentation OverviewPresentation Overview

19

GenScript Overview1

GenScript Services2

GenScript Key Products3

GenScript Facilities4

Business Model5

Your Innovation Partner in Drug Discovery!

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21Your Innovation Partner in Drug Discovery!


GenScript At A Glance Founded in 2002 Over 1300 employees Headquarters in Piscataway, New Jersey USA Facilities and branches:

New Jersey, USA

Hub of big pharmasNanjing, China

Paris, France

Tokyo, Japan

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New Nanjing Bio-Center


325,000 ft2

Capacity expansion: Gene : > 5,000 kb /month Protein : >300 batches / month Antibody: >300 orders/ month

Bioprocess: GLP grade Animal Facility

24

New Bio-Chemical Facility New Peptide Facility 30,000 ft2----200 Shimadzu HPLCs

Capacity expansion: Standard peptide: >6,000/month Pep library: ~50,000 peptides

/month Maintenance : >2,000 peptide

products


25

Structure of Production


Gene Synthesis — Any gene you desire!Largest Gene Supplier in US

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Cloning —Any Cloing you want!

PCR Cloning/SubcloningKI & KO Vector ConstructionCustom vector construction

Mutagenesis — Any variant from your design!

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Low Endotoxin Levels: <1 EU/mg

Molecular Biology Service

Largest Gene Synthesis Supplier !Your Innovation Partner in Drug Discovery! 26

Oligo synthesis —Any oligo you would like

Custom DNA and RNA oligoLargest oligo manufacturer in Asia

One-Stop Protein Services

Deliver Proteins with activityYour Innovation Partner in Drug Discovery!

Protein expression and purification services

Bacteria (Large-scale up to 1000 L )

Yeast(Large-scale up to 1000L)

Insect (Up gram Level){

* High-throughput Protein Variants ServiceGenerating & Selecting Protein Mutants?

GenScript Synthetic Biology Platforms

Up to 1,000 protein variants within 30 business days

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Custom Peptide Synthesis Services

Standard Peptide SynthesisHigh complexity and purityLong peptides up to 200 aaHigh success rate ≥ 95%

Peptide Libraries Rapid parallel platformCompetitive prices Guaranteed deliveryFlexible screening tools

Cosmetic Peptides

Recombinant PeptidesFlexible technologyVariety of modifications Any length, any sequence, any scale

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Leading the industry on peptide delivery success rate!


Antibody Services


Leading the industry on antibody guarantee!

29

30

Animal Facility

Total of 8,000 m2

SPF level for all rodent enclosures Application to

• Rodents (SPF)• Rabbits (Ab production)• Beagle dogs• Non-human primates (cynomolgus)• Individually Ventilated Cages (IVC)

System

Animal Facility


Integrated and Seamless Bio-Assay Center


Given a target, we will delivery HTS-ready assay (w/ reagents)!

Bioprocess R&D


Antibody Drug Development CenterTarget Validation

Antigen Antigen ProductionProduction

Immuni-Immuni-zation zation // Hybridoma

Antibody Engineering

AffinityMaturation

AntibodyProduction

Target Selection

Preclinical Candidate

We Offer Integrated Services to Match Your Needs in Antibody Drug Development

Your Innovation Partner in Drug Discovery! 33


Animal Model Services

We offer comprehensive services to match your needs

eStain® 7-min Protein Staining System


Faster - complete protein gel staining in 7 minutes or less Easier - hit button and run Cleaner - no solutions and buffers required Safer - unique formulation without methanol

2nd Generation eStain® System


WuXi AppTec

We are determined to serve you betterWe are determined to serve you better

38

42

43

CRC Background & Projects Experience

44

EC Liaison & Site Initiation

Subject’s Visits Management

IMP Management

Study File Management

Clinical Sample Management

Safety Affairs

EDC/Data Entry

I. CRC Service

On-Site

Execution,

Coordination,

Management

Of Clinical Trials

Clinical Project Execution & Management

Investigator-Initiated Study

Phase IV/Post-Marketing study

Epidemiology study

Outcome Research

II. Study Management Service

45

Website Website

Community Community ActivitiesActivities

Disease EducationDisease Education

External ReferralExternal Referral

Recruitment Ad.

Internal Referral

Subject Database

III. Patients Education & Management

Subjects recruitment & referral

46

Broad Coverage across China

Henan

Hubei

Hunan

Ningxia

Tibet

Qinghai

Xinjiang

Gansu

Shanxi

Shaanxi

Yunnan

Guizhou

Sichuan

Guangdong

Hainan

Guangxi

Hong Kong

Hebei

Inner Mongolia

Shandong

Anhui

FujianJiangxi

Jiangsu

Zhejiang

Heilongjiang

Jilin

Liaoning

Chongqing

• 40 cities• 135 sites• 130 CRCs

2

2

52

4

5

2

28

1 3

315

64

23

4

5

42

1

3

4

7

3

4

2

2

1

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Think…Learn…Think…Learn…

LINE/LinkedIn: JasonSoung

080-3680-0757

Career

Self Introduction