В.Ф.Сагач Інститут фізіології ім. О.О.Богомольця НАН України

..

. ..

Death Receptors(e.g. Fas, TNF-R1)RadiationDrugs/Chemicals/DiseasesCell surfaceAPOPTOSISNucleusDegradation of DNA andother death substratesGene activation and repressionCaspase 3Caspase 6Caspase 7Caspase 8activationMitochondriaROS(Oxidative stress)NF-kBP53AP-1BaxBcl-2MPT, Ca2+Release of AIF, Cyto C, Apaf-1, Caspases-2,-3,-9 into cytosol

THE SCHEME OF mPTP OPENING

ISCHEMIA-REPERFUSION OF ISOLATED HEART LVP mm HgdP/dt mm Hg/ss

mm Hgmm Hg/s01000-200020001000smm Hgmm Hg/s0100-20000200001000sEffect of mitochondrial permeability transition pore activators phenilarsine oxide and antimycin A on isolated heart function0

Phenilarsine oxide Antimycin A (10-5M) (10-6M)

Absorption spectra of coronary effluents collected during various periods of reperfusion controlpre-treatment withcyclosporin A (10-6 M)Reperfusion:1 - 1st-min, 2 - 2th-min3 - 5th-min, 4 - 40th-min5 - before ischemia 1 - 1st-min, 2 1st-min with CsA pre-treatment3 - before ischemia

Correlation between changes in heart function, oxygen cost of myocardial work and the level of absorbance peaksLVPdP/dtmaxCFOxygen cost of myocardial work

1 - reperfusion 40 min; 2 - reperfusion under the pre-treatment with Trolox (10-6 M);3 - reperfusion under the pre-treatment with cyclosporin A 10-6 M);4 - administration of antimycin A (10-6 M)

mm Hgmm Hgs3000Pre-treatment with trolox decreased postreperfusion heart injury and absorption spectra of coronary effluentsControlTroloxLVPabsorption spectra

Changes in contractile parameters and oxygen cost of myocardial work after 40 min reperfusionLVPCFdP/dtmaxdP/dtmin1 - control, 2 - cyclosporin A 10-6 M, 3 - Trolox 10-6 M (10 min before ischemia)

Oxygen cost of myocardial work

Pre-treatment with melatonin decreased postreperfusion heart injurycontrolmelatoninLVP, mm Hg

Effect of hypoxic precondition on myocardial contraction in guinea pig heartscontrolpreconditioning 15 min X 5, 7 days

Na , 8 40

.. ..

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

1.1

0

25

50

75

100

125

[mm Hg]

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

1.1

1.2

1.3

1.4

1.5

0

25

50

75

100

125

150

175

[mm Hg]

20 . 5 .. .. 2+ , .

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

100.0.0

1.1

1.2

1.3

1.4

1.5

0

50

100

150

[mm Hg]

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

1.1

1.2

0

50

100

150

[mm Hg]

8

0.1120.06350.0140.00611055550.0570.009

1

dP/dt maxdP/dt min

486745671830.112

5.67.26.58.39.10.014

l-NMMA66826880129

5.716.46.83.320.6

626469661580.051

6.90.91.37.417.10.012

1

000

000

000

000

000

l-NMMA

%

2

0005.65.76.9

0007.216.40.9

0006.56.81.3

0008.33.37.4

l-NMMA

%

3

0009.120.617.1

l-NMMA

%

Sheet4

000.0140.012

0.1120.06350.0140.00611055550.0570.009

48.77563.044330579462.45548821515.77472170023.01406653244.0362406744

65.837561.223666546960.60748646897.31558848095.77252619075.4171769358

45.4966.119885837169.5455255726.7170776494.75837814515.3106290876

86.7625104.0694793063122.66648894953.49514650738.2228601413.8703813367

91.497.150072806899.0517075614.41.05179262911.604938067

67.85769.664822880669.07719260558.2852.56231214693.4523148535

%

196.25113.2324394869127.458990926395.026207504710.275146908

t-Test: Paired Two Sample for Means

,%dP/dTmax,%dP/dTmin,%,%..,%dP/dTmax,%dP/dTmin,%,%..

62617012399691278189881931055688

Mean153.4075995132.97766262545514231065627511910081109

Variance466.9944789121411.4753301056514657919470144

Observations88dP/dTmaxdP/dTmin..594355969571149

Pearson Correlation0.860833950263616610497701137273831489780123

Hypothesized Mean Difference0365813546504615410057151

df758647210310675

t Stat5.1941347795675879779663

P(T

2+ % - 1 - 2 - 31

2

3

6

48.77563.044330579462.45548821515.77472170023.01406653244.0362406744

65.837561.223666546960.60748646897.31558848095.77252619075.4171769358

45.4966.119885837169.5455255726.7170776494.75837814515.3106290876

86.7625104.0694793063122.66648894953.49514650738.2228601413.8703813367

91.497.150072806899.0517075614.41.05179262911.604938067

67.85769.664822880669.07719260558.2852.56231214693.4523148535

%

40 .

1

dP/dt maxdP/dt min

486745671830.112

5.67.26.58.39.10.014

l-NMMA66826880129

5.716.46.83.320.6

626469661580.051

6.90.91.37.417.10.012

1

000

000

000

000

000

l-NMMA

%

2

0005.65.76.9

0007.216.40.9

0006.56.81.3

0008.33.37.4

l-NMMA

%

3

0009.120.617.1

l-NMMA

%

Sheet4

000.0140.012

0.1120.06350.0140.00611055550.0570.009

48.77563.044330579462.45548821515.77472170023.01406653244.0362406744

65.837561.223666546960.60748646897.31558848095.77252619075.4171769358

45.4966.119885837169.5455255726.7170776494.75837814515.3106290876

86.7625104.0694793063122.66648894953.49514650738.2228601413.8703813367

91.497.150072806899.0517075614.41.05179262911.604938067

67.85769.664822880669.07719260558.2852.56231214693.4523148535

%

196.25113.2324394869127.458990926395.026207504710.275146908

t-Test: Paired Two Sample for Means

,%dP/dTmax,%dP/dTmin,%,%..,%dP/dTmax,%dP/dTmin,%,%..

62617012399691278189881931055688

Mean153.4075995132.97766262545514231065627511910081109

Variance466.9944789121411.4753301056514657919470144

Observations88dP/dTmaxdP/dTmin..594355969571149

Pearson Correlation0.860833950263616610497701137273831489780123

Hypothesized Mean Difference0365813546504615410057151

df758647210310675

t Stat5.1941347795675879779663

P(T

2+ (5*10-3 ) NOS arginase *NOSNO2Arginase** * 2+2.Uric acid* ** ** *

7

10071.860465116373.72093023265.58139534885.581395348811.041009463711.041009463712.297734627812.2977346278

(10-6 )

Ca 2+ (5 * 10-3 M)

%

1

()

1. .

2*- . -NO3-R3NO2-PiiNOScNOSNOSS

30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

NO2-Ca 2+ (5 * 10-3 M) (10-6 )

66.38134.791.61

3.318.520.5

%%100202.9225670383138.008436276

4.971376920813.734224201922.3774697085

4.33.173.09

0.240.350.38

%%10073.720930232671.8604651163

5.581395348811.041009463712.2977346278

0004.97137692084.971376920813.734224201913.734224201922.377469708522.3774697085

Ca 2+ (5 * 10-3 M)

(10-6 )

3

0005.58139534885.581395348811.041009463711.041009463712.297734627812.2977346278

Ca 2+ (5 * 10-3 M)

(10-6 )

1

3.850.890.770.13

2.792.30.530.49

3.892.771.010.97

iNOS

cNOS

/

1

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

NO2- (10-6 )Ca 2+ (5 * 10-3 M)2*- (10-6 )Ca 2+ (5 * 10-3 M)iNOSCa 2+ (5 * 10-3 M)

66.3891.61134.7174.796.1978.923.852.793.89

3.320.518.511.549.121.20.770.531.01

%%100138.008436276202.922567038310055.060103033845.1745850029

4.971376920822.377469708513.73422420196.60560961659.460442873526.8626457172

4.33.093.17. -12.927.577.63cNOS0.892.32.77

0.240.380.351.460.850.70.130.490.97

%%10071.860465116373.720930232610058.591331269359.0557275542

5.581395348812.297734627811.041009463711.300309597511.22853368569.1743119266

100138.008436276202.92256703834.97137692084.971376920813.734224201913.734224201922.377469708522.3774697085

(10-6 )

Ca 2+ (5 * 10-3 M)

3

10071.860465116373.72093023265.58139534885.581395348811.041009463711.041009463712.297734627812.2977346278

(10-6 )

Ca 2+ (5 * 10-3 M)

10058.591331269359.055727554211.300309597511.22853368569.1743119266

(10-6 )

Ca 2+ (5 * 10-3 M)

10055.060103033845.17458500296.60560961659.460442873526.8626457172

(10-6 )

Ca 2+ (5 * 10-3 M)

3.850.890.770.13

2.792.30.530.49

3.892.771.010.97

iNOS

cNOS

/

2

10058.591331269359.055727554211.300309597511.22853368569.1743119266

(10-6 )

Ca 2+ (5 * 10-3 M)

%

1

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

NO2- (10-6 )Ca 2+ (5 * 10-3 M)2*- (10-6 )Ca 2+ (5 * 10-3 M)

66.3891.61134.7174.796.1978.92

3.320.518.511.549.121.2

%%100138.008436276202.922567038310055.060103033845.1745850029

4.971376920822.377469708513.73422420196.60560961659.460442873526.8626457172

4.33.093.17. -12.927.577.63

0.240.380.351.460.850.7

%%10071.860465116373.720930232610058.591331269359.0557275542

5.581395348812.297734627811.041009463711.300309597511.22853368569.1743119266

0004.97137692084.971376920813.734224201913.734224201922.377469708522.3774697085

(10-6 )

Ca 2+ (5 * 10-3 M)

3

0005.58139534885.581395348811.041009463711.041009463712.297734627812.2977346278

(10-6 )

Ca 2+ (5 * 10-3 M)

00011.300309597511.22853368569.1743119266

(10-6 )

Ca 2+ (5 * 10-3 M)

0006.60560961659.460442873526.8626457172

(10-6 )

Ca 2+ (5 * 10-3 M)

4

100138.008436276202.92256703834.97137692084.971376920813.734224201913.734224201922.377469708522.3774697085

(10-6 )

Ca 2+ (5 * 10-3 M)

%

1

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

NO2-Ca 2+ (5 * 10-3 M) (10-6 )

66.38134.791.61

3.318.520.5

%%100202.9225670383138.008436276

4.971376920813.734224201922.3774697085

4.33.173.09

0.240.350.38

%%10073.720930232671.8604651163

5.581395348811.041009463712.2977346278

0004.97137692084.971376920813.734224201913.734224201922.377469708522.3774697085

Ca 2+ (5 * 10-3 M)

(10-6 )

3

0005.58139534885.581395348811.041009463711.041009463712.297734627812.2977346278

Ca 2+ (5 * 10-3 M)

(10-6 )

1

10055.060103033845.17458500296.60560961659.460442873526.8626457172

(10-6 )

Ca 2+ (5 * 10-3 M)

%

1

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

()

1. .


30 .//////

M174.712.9251.495.544.366.381.423.850.894.751.67

m11.541.465.130.480.243.30.140.770.130.760.42

n =5

Ca 2+ (5 * 10-3 M)

M78.927.6343.475.033.17134.71.183.892.776.661.78

m21.2*0.7*8.230.350.35*18.5*0.151.010.970.540.15*

t 13.9683.2670.8260.7912.6623.6351.1690.0311.922.0480.246

n =3

(10-6 )

M96.197.5738.083.873.0991.611.212.792.35.11.98

m9.1*0.85*6.950.580.38*20.50.320.530.49*0.430.22

t 25.3463.1161.5522.2182.6921.2150.6011.1332.7810.40.653

n =6

*- (M+/-m)

NO2- (10-6 )Ca 2+ (5 * 10-3 M)2*- (10-6 )Ca 2+ (5 * 10-3 M)

66.3891.61134.7174.796.1978.92

3.320.518.511.549.121.2

%%100138.008436276202.922567038310055.060103033845.1745850029

4.971376920822.377469708513.73422420196.60560961659.460442873526.8626457172

4.33.093.17. -12.927.577.63

0.240.380.351.460.850.7

%%10071.860465116373.720930232610058.591331269359.0557275542

5.581395348812.297734627811.041009463711.300309597511.22853368569.1743119266

0004.97137692084.971376920813.734224201913.734224201922.377469708522.3774697085

(10-6 )

Ca 2+ (5 * 10-3 M)

3

0005.58139534885.581395348811.041009463711.041009463712.297734627812.2977346278

(10-6 )

Ca 2+ (5 * 10-3 M)

00011.300309597511.22853368569.1743119266

(10-6 )

Ca 2+ (5 * 10-3 M)

0006.60560961659.460442873526.8626457172

(10-6 )

Ca 2+ (5 * 10-3 M)

Effects NO on heart oxygen consumption

I - L-arginine II - Sodium nitroprusside III - L-NMMA- control- agents

2+ RR (2) CsA (3) 2+ 60 2+ NO

6

000

303240

676480

8884120

9896160

100100100

10085100

936998

8049.898

6536.796

5325.296

3915.294

27894

170520

7560560

0600600

RR

CsA

1

2

3

1

2

3

t, c

[Ca2+]m, %

1

1234123123

05401004600000000

20322872402024405520204151

40851.5494140396675.540446383

60368322960508085.760637694

8008020.3198057.78791.7807379.498

1000871311.5100639096100788098.3

1209287.11206812081

123

0.025

123412340.005

120.3560.250.015

040400.2010.1850.025

2019.4220.10.12430.03

4017.6160.040.08930.035

0.010.06120.04

0.060.04080.045

0.1750.0530.02720.049

0.2350.0130.01460.051

0.2750.0190.00780.055

0.30.0390.00390.05

0.3150.0770.0070.160.025

0.3250.1240.040.02520.2250.005

0.330.1660.010.050.2650.013

0.3350.1920.060.0760.290.024

123450.340.2090.150.040.1050.0370.3050.031

0.110.1120.3430.220.1560.010.1250.0250.3150.038

0.1020.10560.2270.1590.060.150.0190.320.040.015

0.0560.1080.2280.1590.1450.170.0250.0420.01

0.030.09170.1520.190.050.060.015

0.0220.07880.1120.1530.2050.07280.0950.017

0.0220.06830.1040.1530.21750.0770.150.018

0.060.1080.22910.0770.1670.1850.017

0.0550.070.2330.0770.1160.20.015

0.0530.060.1120.240.0850.210.035

0.0510.0550.1080.2430.06210.2150.06

0.0520.1080.0470.2170.085

0.060.0370.105

0.0550.1120.030.12

0.05280.10640.0250.132

0.05130.10760.020.142

0.09710.0190.145

0.0760.01360.15

0.07460.0107

0.07460.0107

1

0000

0000

0000

0000

0000

0000

0000

t,

1

2

3

4

1

2

3

4

2+, %

2

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

A654, %

3

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

520, %

004455

00

00

t1/2,

1

2

1

2

2+

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

,

2+

520,0,05 .

2+

2+

2+

3

,

,

,

1

2

3

4

5

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

Ca2+

,

2+

3

654,0,1 .

1

2

3

4

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

Sr2+

,

1

2

3

Sr2+

654,0,1 .

3

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1,2,3

A23187

A23187

1- CCCP,2- RR,3- 2+

3

654,0,1.

1

2

1,2

2+ 23187

1231234020401234123

000000000127.517.69.901001001001000000

20131212021.12016.7022413.282087798690200.340.140.09

4024221404231400326.510.8540765872.784400.690.30.2

6036341606138521430.5153.56064396072601.050.450.33

8047.3422807444521528.9142.48054.6264862801.430.620.45

100564221008346532625.2106.6100441736531001.770.810.62

120654331209048532120351027431202.20.980.75

14073433140934953414027721341402.691.160.93

160794441609654416021415261601.331.05

18084444180985441801621220

2008844420010054200120815

2209252208511

240945240636

26096260414

28098280102

30010030000

123

000

403032

806764

1208884

0551609896

2034200100100100

401024010085100

604280936998

80012343208049.898

1234000003606536.796

0100100100100200.160.160.260.224005325.296

2082.6867780400.3850.330.580.544403915.294

4063.868.35658600.710.650.890.9448027894

6044.949.14139801.081.081.171.38520170

803432.53126.41001.431.561.511.665607

100242122191201.71.971.832.126000

1201814161214022.32.22.5

1401391071602.4

16095631802.8

1806322200

2004100

22010

123

12312304.64.6

04.64.604.64.6104.564.38

104.564.38104.564.4204.354.01

204.354.01204.354.02304.113.77

304.113.77304.113.76403.93.533.53

403.93.533.53403.93.51503.673.25

503.673.3503.673.243.24603.433.08

603.433.08603.432.96703.172.8

703.172.8703.172.8802.962.61

802.962.58802.962.53902.732.39

902.73902.731002.532.23

1002.531002.531102.31

1102.311102.311202.11

1202.111202.111301.89

1301.821301.82140

140140

150

160

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

B

RR

1

2

3

t, s

% of total Sr2+

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

4

3

2

RR

A

1

2

3

4

t,

[Ca2+]o,%

0

0

0

0

0

t,

Ca2+,%

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s

matrix Me2+ content, %

000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s

ln A0/A

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

A

k=1,5.10-2 c-1

t1/2=46 c

1

2

3

4

t, s

[Ca2+]m, %

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

B

1

2

3

4

t,s

ln A0/A

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data: N6,7,85.07.05

1

2

3

t, c

ln C

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data:N2, 5.07.05

1

2

3

t, c

ln C

y1 = -0,278x + 4,8947R2 = 0,9953

y2 = -0,229x + 4,6R2 = 0,9899

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

t, c

ln C

00

00

00

1

2

1

2

1

2

1- 2- 2+

5

6

[Ca2+]o,

t1/2, c

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

RR

CsA

1

2

3

2+

1

2

3

t, c

[Ca2+]m, %

12121

010010004.64.603.27

109678.4104.564.38102.98

207855204.354.01202.22

306140304.113.7301.8

404931.4403.93.44401.53

503924503.673.17501.3

603118.9603.43360

702416703.172.8

801913802.962.58

90159902.732.32

1001371002.532.07

1101051102.31

120831202.11

130621301.9

14051140

15030150

1602160

1701

1800

190

200

1231231

0100100100010010010004

108074.3870.47207575.1868.73203.4

205556.0554.33406161.5657.96402.77

304342.6942.58605454.152.58602.2

403432.8233.5780515049.37801.8

502725.4126.541004947.747.241001.4

602219.7921.011204845.8245.72

701615.4816.651404645.2544.59

801312.1613.21604543.71

9099.5710.471804343.01

10077.58.3200

110566.6

12034.75.2

13023.74.1

140133.3

15002.42.6

1601.82.1

1701.51.6

1801.21.3

1900.91121234

20004.64.60100100100100

12204.014.252079.2555442.6

0100100403.53.724050.5343415.3

205570603.083.166028.722215.1

403441802.582.52801913132

6022241001.961.7910013781

8013131201208350

10075.5140513

12033160202

1401018001

1600200

180

1234

0

0.046

0.098

0.1270

0.150.049

0.1640.101

0.1750.138

0.1830.1590

0.1880.1730.027

0.1840.048

0.190.071

0.1930.0850

0.0940.01

0.0980.008

0.1020.011

0.1060.012

0.012

0.012

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t,

[Ca2+m], %

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

ln C

0

0

0

0

0

0

0

1

t, c

ln[C-C2exp(-k2t)]

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

B

1

2

3

t, c

[Ca2+m], %

000

000

000

000

000

000

000

000

000

000

000

1

2

3

t, c

[Ca2+m], %

0

0

0

0

0

0

1

t, c

ln (C-C1)

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

[Ca2+m], %

00

00

00

00

00

00

00

1

2

t, c

ln C

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

2

654,0,1 .

(1)

(2)

(1)

(2)

(1)

(2)

(3)

1

2

3

4

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

2

3

4

t, c

[Ca2+]m, %

1

000

0.0210.0290.001

0.0530.0440.005

0.0750.0530.008

0.0970.060.016

0.1170.0680.021

0.130.0750.029

0.1430.0820.035

0.1530.0880.0471

0.1620.0940.06

0.170.10.066

0.177110.072

0.183120.08

0.188130.087

141414

2+

1

2

3

1-RR2-RR, Ca2+3-Ca2+, RR

1

2

3

t,

[2+]o, .

1

12312300.0070.0170.0340.050.150.41234

0000000011002301100754530100100

10.0210.0290.00110.0240.0260.0022100524047100224.939.460101.4120100

20.0530.0440.00520.050.0480.0023100979592550352082.896.414068

30.0750.0530.00830.0760.0740.004410094939293909016055

40.0970.060.01640.0960.080.00418042

50.1170.0680.02150.1160.0820.00620015

60.130.0750.02960.130.0840.006

70.1430.0820.03570.1420.0860.006

80.1530.0880.047180.1520.0880.008

90.1620.0940.0690.1640.0920.008

100.170.10.066100.1720.0940.008

110.1770.072110.1780.095

120.1830.08120.184

130.1880.087130.188

1414

15

16

1234

00000

10.0210.02800

-6-5.5-5-4.5-4-3.5-3-2.5-6-5.5-5-4.5-4-3.5-320.0410.0440.0030.001

100.0150.0210.040.070.0920.0980.09811004512030.0630.05670.0130.005

200.010.030.0350.0650.0950.09721006545425040.08430.060.0240.009

300.0050.0350.0750.075310097959040050.10080.06660.0370.016

4100939395929060.1170.07370.0470.021

70.130.07790.06240.029

80.14030.08450.0740.035

90.14810.0810.042

100.15450.0880.0517

110.0950.0609

120.1020.0687

130.1090.0765

140.1140.0836

150.1190.088

160.124

170.127

18

1

1001001001001010775555

23529794

0409593

0.034479292

0.05105593

0.150090

1

2

3

4

%

1

2

3

4

2

1001001001001010885566

45659793

12459593

0429095

-454092

-3.50090

%

A540/

1

2

3

1

2

3

lg [M]

-6

-6

-6

-5.5

-5.5

-5.5

-5

-5

-5

-4.5

-4.5

-4.5

-4

-4

-4

-3.5

-3.5

-3.5

-3

-3

-3

-2.5

-2.5

-2.5

0

0.007

0.007

0.006

0.006

0.003

0.003

0.015

0

0.021

0.01

0.04

0.03

0

0.07

0.035

0.005

0.092

0.065

0.035

0.098

0.095

0.075

0.098

0.097

0.075

, %

1

2

3

4

1

2

3

4

lg [M]

3

10024.95

7539.420

456082.8

30101.496.4

1

2

3

,

,%

100

100

68

55

42

15

A654,%

[Ca2+],

0000

0.0210.02800

0.0410.0440.0030.001

0.0630.05670.0130.005

0.08430.060.0240.009

0.10080.06660.0370.016

0.1170.07370.0470.021

0.130.07790.06240.029

0.14030.08450.0740.035

0.148190.0810.042

0.1545100.0880.0517

11110.0950.0609

12120.1020.0687

13130.1090.0765

14140.1140.0836

15150.1190.088

16160.12416

17170.12717

18181818

Ca2+

1

2

3

4

1

2

3

4

t,

A654

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

2+

1

2

3


1

2

3

t,

[2+]o, .

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

NO-

1

2

3

1

2

3

t,

, ..

2

000

0.0240.0260.002

0.050.0480.002

0.0760.0740.004

0.0960.080.004

0.1160.0820.006

0.130.0840.006

0.1420.0860.006

0.1520.0880.008

0.1640.0920.008

0.1720.0940.008

0.1780.09511

0.1841212

0.1881313

141414

NO-

1

2

3

1-RR2-RR, NO3-NO, RR

1

2

3

t,

[Ca2+]o, .

1

12312300.0070.0170.0340.050.150.41234

0000000011002301100754530100100

10.0210.0290.00110.0240.0260.0022100524047100224.939.460101.4120100

20.0530.0440.00520.050.0480.0023100979592550352082.896.414068

30.0750.0530.00830.0760.0740.004410094939293909016055

40.0970.060.01640.0960.080.00418042

50.1170.0680.02150.1160.0820.00620015

60.130.0750.02960.130.0840.006

70.1430.0820.03570.1420.0860.006

80.1530.0880.047180.1520.0880.008

90.1620.0940.0690.1640.0920.008

100.170.10.066100.1720.0940.008

110.1770.072110.1780.095

120.1830.08120.184

130.1880.087130.188

1414

15

16

1234

00000

10.0210.02800

-6-5.5-5-4.5-4-3.5-3-2.5-6-5.5-5-4.5-4-3.5-320.0410.0440.0030.001

100.0150.0210.040.070.0920.0980.09811004512030.0630.05670.0130.005

200.010.030.0350.0650.0950.09721006545425040.08430.060.0240.009

300.0050.0350.0750.075310097959040050.10080.06660.0370.016

4100939395929060.1170.07370.0470.021

70.130.07790.06240.029

80.14030.08450.0740.035

90.14810.0810.042

100.15450.0880.0517

110.0950.0609

120.1020.0687

130.1090.0765

140.1140.0836

150.1190.088

160.124

170.127

18

1

1001001001001010775555

23529794

0409593

0.034479292

0.05105593

0.150090

1

2

3

4

%

1

2

3

4

2

1001001001001010885566

45659793

12459593

0429095

-454092

-3.50090

%

A540/

1

2

3

1

2

3

lg [M]

-6

-6

-6

-5.5

-5.5

-5.5

-5

-5

-5

-4.5

-4.5

-4.5

-4

-4

-4

-3.5

-3.5

-3.5

-3

-3

-3

-2.5

-2.5

-2.5

0

0.007

0.007

0.006

0.006

0.003

0.003

0.015

0

0.021

0.01

0.04

0.03

0

0.07

0.035

0.005

0.092

0.065

0.035

0.098

0.095

0.075

0.098

0.097

0.075

, %

1

2

3

4

1

2

3

4

lg [M]

3

10024.95

7539.420

456082.8

30101.496.4

1

2

3

,

,%

100

100

68

55

42

15

A654,%

[Ca2+],

0000

0.0210.02800

0.0410.0440.0030.001

0.0630.05670.0130.005

0.08430.060.0240.009

0.10080.06660.0370.016

0.1170.07370.0470.021

0.130.07790.06240.029

0.14030.08450.0740.035

0.148190.0810.042

0.1545100.0880.0517

11110.0950.0609

12120.1020.0687

13130.1090.0765

14140.1140.0836

15150.1190.088

16160.12416

17170.12717

18181818

Ca2+

1

2

3

4

1

2

3

4

t,

A654

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

2+

1

2

3


1

2

3

t,

[2+]o, .

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

NO-

1

2

3


NO

1

2

3

t,

[Ca2+]o, .

2

0

0.028

0.044

0.058

0.0668

0.0723

0.078

0.0818

0.085

0.088

Km=50

Km=50

1

[Ca2+],

v, A520.-1

1

12312300.0070.0170.0340.050.150.4123400.020.040.060.080.10.120.140.160.181

0000000011002301100754530100100100.0280.0440.0580.06680.07230.0780.08180.0850.0880.250.1

10.0210.0290.00110.0240.0260.0022100524047100224.939.460101.41201000.50.08

20.0530.0440.00520.050.0480.0023100979592550352082.896.4140680.750.07

30.0750.0530.00830.0760.0740.00441009493929390901605510.06

40.0970.060.01640.0960.080.004180421.250.04

50.1170.0680.02150.1160.0820.006200151.50.038

60.130.0750.02960.130.0840.0061.750.016

70.1430.0820.03570.1420.0860.0062

80.1530.0880.047180.1520.0880.0082.25

90.1620.0940.0690.1640.0920.008

100.170.10.066100.1720.0940.008

110.1770.072110.1780.095

120.1830.08120.184

130.1880.087130.188

1414

15

16

1234

00000

10.0210.02800

-6-5.5-5-4.5-4-3.5-3-2.5-6-5.5-5-4.5-4-3.5-320.0410.0440.0030.001

100.0150.0210.040.070.0920.0980.09811004512030.0630.05670.0130.005

200.010.030.0350.0650.0950.09721006545425040.08430.060.0240.009

300.0050.0350.0750.075310097959040050.10080.06660.0370.016

4100939395929060.1170.07370.0470.021

70.130.07790.06240.029

80.14030.08450.0740.035

90.14810.0810.042

100.15450.0880.0517

110.0950.0609

120.1020.0687

130.1090.0765

140.1140.0836

150.1190.088

160.124

170.127

18

1

00001010775555

0000

0000

0000

0000

0000

1

2

3

4

%

1

2

3

4

2

00001010885566

0000

0000

0000

0000

0000

%

A540/

1

2

3

1

2

3

lg [M]

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0.007

0.007

0.006

0.006

0.003

0.003

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

, %

1

2

3

4

1

2

3

4

lg [M]

3

1

2

3

,

,%

A654,%

[Ca2+],

Ca2+

1

2

3

4

1

2

3

4

t,

A654

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

2+

1

2

3


1

2

3

t,

[2+]o, .

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

NO-

1

2

3


NO

1

2

3

t,

[Ca2+]o, .

Km=50

Km=50

1

[Ca2+],

v, A520.-1

0

0

0

0

0

0

0

1

v/[Ca2+], -1.-1

v, A520.-1

NO NO , ..dP/dt, ../

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

2.2

2.4

0

25

50

75

-1000

0

1000

2000

- bax 1 - 2 - 1 , 2 5 , 3 40 bax, 487 .. M 1 1 2 2 3 3 M 1 1 2 2 3 3

2+ 1, 3 - ; 2, 4 -

2+ - - 1 ; 2

2-, H2O2 2-H2O21 - 2 -

NOS 2+- 1, 2 - ; 3, 4 - L-NAME+2+L-NAME+-Na+2+2+

- 1 2 (10-5/); 3 (10-5/), (10-5/).

22, 2- .

1 2 22.2-

1

5.560.471.95

23.759.183.93

/

Sheet1

5.560.47

23.753.93

9.181.95

1.180.22

3.690.81

1.170.09

23.753.93

5.560.47

Sheet1

0

0

0

0

/

Sheet2

00

0

Sheet3

000.471.95

003.93

/

000.220.09

000.81

*10(-2)/30*

2

1.180.220.09

3.691.170.81

*10(-2)/30*

Sheet1

5.560.47

23.753.93

9.181.95

1.180.22

3.690.81

1.170.09

23.753.93

5.560.47

Sheet1

0

0

0

0

/

Sheet2

00

0

Sheet3

000.471.95

003.93

/

000.220.09

000.81

*10(-2)/30*

1 - 2 - dP/dt1212

2+ - 2+ + 2+

- bax NO- 1 2 3 4 1 2 3 4487 .. bax

6-

( 90%) -

1 control

(n=197)

2 hypoxia, precondition, 1 month (n=7)

5 trolox intraperitaneal (10 + 5 mg/kg) (n=25)

3 - hypoxia, postcondition, 3 days (n=12)

6 trolox per os (10 mg/kg)

(n=35)

4 - hypoxia, pre- and postcondition (n=16)

7 melatonine intraperitaneal (10 mg/kg) (n=12)

_1197974617.bin

1

0.080.0180.018

0.2150.0250.025

0.280.030.03

0.2650.040.04

0.350.050.05

D

1

Control0.080.0180.018

10 min0.2150.0250.025

90 min0.280.030.03

2 min0.2650.040.04

5 min0.350.050.05

1

0

0

0

0

0

0

0

D

D

2

3

2

0.9656482075

0.9201371873

0.8450224979

0.8059033412

coefficient of correlation, r

1

Control0.080.0180.018

10 min0.2150.0250.025

90 min0.280.030.03

2 min0.2650.040.04

5 min0.350.050.05

1

00.0180.018

0

00.0250.025

00.030.03

0

00.040.04

00.050.05

artificial circulation

reperfusion

D

4

0.0650.0043301270.004330127

0.1450.0350.035

Sex

Source value

1

0.8586157594

0.8256644514

0.813253534


2

.Male0.0650.0043301270.004330127

.Feminine0.1450.0350.035

400.30428571430.04512373660.0451237366

310.59

330.58

370.52

510.23

530.52

550.36

570.22

590.41

600.23

660.16

Sex0.8586157594

Age0.8256644514

ECG0.813253534

CPK0.9656482075

mvCPK0.9201371873

AST0.8450224979

LDG0.8059033412

2

0

0

0

0


3

3

0.550.01784708710.0178470871

0.30428571430.04512373660.0451237366

AGE

Max. value

/ 1 2 -

(150 /) (210-5/)

- 1 - 2 - 3 - l - NAME4 - t(BOOH)5 -6 - +7 - + l-* - p

1 - 2 - 3 - l - NAME 4 - t(BOOH)5 - 6 - + 7 - + l-

` (10-12 ) (1012 )` (1012 ) L-

( , dP/dt max, dP/dt min)

( , dP/dt max, dP/dt min)

2+, , t-BuOOH 2+, a mt-NOS NO a mt-NOS NO

(22;2-) () (22;2-) () m- bax m- bax

2+ m 2+ 23187 - 2+ 1 CCCP; 2 CCCP, CsA; 3 . CCCP (1); CCCP, RR (2, 4); CsA, RR, CCCP (3). 2+ (1) 2+ (2)

3

0000

21.12016.70

4231400

6138521

7444521

8346532

9048532

9349534

96160544

98180544

10020054200

1

4

3

2

RR

A

1

2

3

4

t,

[Ca2+]o,%

1

1234123123

05401004600000000

20322872402024405520204151

40851.5494140396675.540446383

60368322960508085.760637694

8008020.3198057.78791.7807379.498

1000871311.5100639096100788098.3

1209287.11206812081

123

0.025

123412340.005

120.3560.250.015

040400.2010.1850.025

2019.4220.10.12430.03

4017.6160.040.08930.035

0.010.06120.04

0.060.04080.045

0.1750.0530.02720.049

0.2350.0130.01460.051

0.2750.0190.00780.055

0.30.0390.00390.05

0.3150.0770.0070.160.025

0.3250.1240.040.02520.2250.005

0.330.1660.010.050.2650.013

0.3350.1920.060.0760.290.024

123450.340.2090.150.040.1050.0370.3050.031

0.110.1120.3430.220.1560.010.1250.0250.3150.038

0.1020.10560.2270.1590.060.150.0190.320.040.015

0.0560.1080.2280.1590.1450.170.0250.0420.01

0.030.09170.1520.190.050.060.015

0.0220.07880.1120.1530.2050.07280.0950.017

0.0220.06830.1040.1530.21750.0770.150.018

0.060.1080.22910.0770.1670.1850.017

0.0550.070.2330.0770.1160.20.015

0.0530.060.1120.240.0850.210.035

0.0510.0550.1080.2430.06210.2150.06

0.0520.1080.0470.2170.085

0.060.0370.105

0.0550.1120.030.12

0.05280.10640.0250.132

0.05130.10760.020.142

0.09710.0190.145

0.0760.01360.15

0.07460.0107

0.07460.0107

1

0000

0000

0000

0000

0000

0000

0000

t,

1

2

3

4

1

2

3

4

2+, %

2

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

A654, %

3

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

520, %

004455

00

00

t1/2,

1

2

1

2

2+

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

,

2+

520,0,05 .

2+

2+

2+

3

,

,

,

1

2

3

4

5

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

Ca2+

,

2+

3

654,0,1 .

1

2

3

4

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

Sr2+

,

1

2

3

Sr2+

654,0,1 .

3

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

2

3

A23187

A23187

A23187

1- CCCP,2- RR,3- 2+

3

654,0,1 .

1

2

3

1

1231234020401234123

000000000127.517.69.901001001001000000

20131212021.12016.7022413.282087798690200.340.140.09

4024221404231400326.510.8540765872.784400.690.30.2

6036341606138521430.5153.56064396072601.050.450.33

8047.3422807444521528.9142.48054.6264862801.430.620.45

100564221008346532625.2106.6100441736531001.770.810.62

120654331209048532120351027431202.20.980.75

14073433140934953414027721341402.691.160.93

160794441609654416021415261601.331.05

18084444180985441801621220

2008844420010054200120815

2209252208511

240945240636

26096260414

28098280102

30010030000

055

2034

4010

604

8001234

123400000

0100100100100200.160.160.260.22

2082.6867780400.3850.330.580.54

4063.868.35658600.710.650.890.94

6044.949.14139801.081.081.171.38

803432.53126.41001.431.561.511.66

100242122191201.71.971.832.12

1201814161214022.32.22.5

1401391071602.4

16095631802.8

1806322200

2004100

22010

123

12312304.64.6

04.64.604.64.6104.564.38

104.564.38104.564.4204.354.01

204.354.01204.354.02304.113.77

304.113.77304.113.76403.93.533.53

403.93.533.53403.93.51503.673.25

503.673.3503.673.243.24603.433.08

603.433.08603.432.96703.172.8

703.172.8703.172.8802.962.61

802.962.58802.962.53902.732.39

902.73902.731002.532.23

1002.531002.531102.31

1102.311102.311202.11

1202.111202.111301.89

1301.821301.82140

140140

150

160

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

B

RR

1

2

3

t, s

% of total Sr2+

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

4

3

2

RR

A

1

2

3

4

t,

[Ca2+]o,%

0

0

0

0

0

t,

Ca2+,%

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s


000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s

ln A0/A

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

A

k=1,5.10-2 c-1

t1/2=46 c

1

2

3

4

t, s

[Ca2+]m, %

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

B

1

2

3

4

t,s

ln A0/A

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data: N6,7,85.07.05

1

2

3

t, c

ln C

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data:N2, 5.07.05

1

2

3

t, c

ln C

y1 = -0,278x + 4,8947R2 = 0,9953

y2 = -0,229x + 4,6R2 = 0,9899

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

t, c

ln C

00

00

00

1

2

1

2

1

2

1- 2- 2+

5

6

[Ca2+]o,

t1/2, c

12121

010010004.64.603.27

109678.4104.564.38102.98

207855204.354.01202.22

306140304.113.7301.8

404931.4403.93.44401.53

503924503.673.17501.3

603118.9603.43360

702416703.172.8

801913802.962.58

90159902.732.32

1001371002.532.07

1101051102.31

120831202.11

130621301.9

14051140

15030150

1602160

1701

1800

190

200

1231231

0100100100010010010004

108074.3870.47207575.1868.73203.4

205556.0554.33406161.5657.96402.77

304342.6942.58605454.152.58602.2

403432.8233.5780515049.37801.8

502725.4126.541004947.747.241001.4

602219.7921.011204845.8245.72

701615.4816.651404645.2544.59

801312.1613.21604543.71

9099.5710.471804343.01

10077.58.3200

110566.6

12034.75.2

13023.74.1

140133.3

15002.42.6

1601.82.1

1701.51.6

1801.21.3

1900.91121234

20004.64.60100100100100

12204.014.252079.2555442.6

0100100403.53.724050.5343415.3

205570603.083.166028.722215.1

403441802.582.52801913132

6022241001.961.7910013781

8013131201208350

10075.5140513

12033160202

1401018001

1600200

180

1234

0

0.046

0.098

0.1270

0.150.049

0.1640.101

0.1750.138

0.1830.1590

0.1880.1730.027

0.1840.048

0.190.071

0.1930.0850

0.0940.01

0.0980.008

0.1020.011

0.1060.012

0.012

0.012

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t,

[Ca2+m], %

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

ln C

0

0

0

0

0

0

0

1

t, c

ln[C-C2exp(-k2t)]

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

B

1

2

3

t, c

[Ca2+m], %

000

000

000

000

000

000

000

000

000

000

000

1

2

3

t, c

[Ca2+m], %

0

0

0

0

0

0

1

t, c

ln (C-C1)

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

[Ca2+m], %

00

00

00

00

00

00

00

1

2

t, c

ln C

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

2

654,0,1 .

(1)

(2)

(1)

(2)

(1)

(2)

(3)

1

2

3

4

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

2

3

4

t, c

[Ca2+]m, %

1

0.025

0.005

0.015

0.025

0.03

0.035

0.04

0.045

0.049

0.051

0.055

0.05

0.160.025

0.2250.005

0.2650.013

0.290.024

0.3050.031

0.3150.038

0.320.04

0.042

0.06

0.095

0.15

0.185

0.2

0.21

0.215

0.217

1,2,3

A23187

A23187

1- CCCP,2- RR,3- 2+

3

654,0,1.

1

2

1,2

1

1234123123

05401004600000000

20322872402024405520204151

40851.5494140396675.540446383

60368322960508085.760637694

8008020.3198057.78791.7807379.498

1000871311.5100639096100788098.3

1209287.11206812081

123

0.025

123412340.005

120.3560.250.015

040400.2010.1850.025

2019.4220.10.12430.03

4017.6160.040.08930.035

0.010.06120.04

0.060.04080.045

0.1750.0530.02720.049

0.2350.0130.01460.051

0.2750.0190.00780.055

0.30.0390.00390.05

0.3150.0770.0070.160.025

0.3250.1240.040.02520.2250.005

0.330.1660.010.050.2650.013

0.3350.1920.060.0760.290.024

123450.340.2090.150.040.1050.0370.3050.031

0.110.1120.3430.220.1560.010.1250.0250.3150.038

0.1020.10560.2270.1590.060.150.0190.320.040.015

0.0560.1080.2280.1590.1450.170.0250.0420.01

0.030.09170.1520.190.050.060.015

0.0220.07880.1120.1530.2050.07280.0950.017

0.0220.06830.1040.1530.21750.0770.150.018

0.060.1080.22910.0770.1670.1850.017

0.0550.070.2330.0770.1160.20.015

0.0530.060.1120.240.0850.210.035

0.0510.0550.1080.2430.06210.2150.06

0.0520.1080.0470.2170.085

0.060.0370.105

0.0550.1120.030.12

0.05280.10640.0250.132

0.05130.10760.020.142

0.09710.0190.145

0.0760.01360.15

0.07460.0107

0.07460.0107

1

0000

0000

0000

0000

0000

0000

0000

t,

1

2

3

4

1

2

3

4

2+, %

2

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

A654, %

3

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

520, %

004455

00

00

t1/2,

1

2

1

2

2+

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

,

2+

520,0,05 .

2+

2+

2+

3

,

,

,

1

2

3

4

5

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

Ca2+

,

2+

3

654,0,1 .

1

2

3

4

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

Sr2+

,

1

2

3

Sr2+

654,0,1 .

3

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1,2,3

A23187

A23187

1- CCCP,2- RR,3- 2+

3

654,0,1.

1

2

1,2

2+ 23187

1231234020401234123

000000000127.517.69.901001001001000000

20131212021.12016.7022413.282087798690200.340.140.09

4024221404231400326.510.8540765872.784400.690.30.2

6036341606138521430.5153.56064396072601.050.450.33

8047.3422807444521528.9142.48054.6264862801.430.620.45

100564221008346532625.2106.6100441736531001.770.810.62

120654331209048532120351027431202.20.980.75

14073433140934953414027721341402.691.160.93

160794441609654416021415261601.331.05

18084444180985441801621220

2008844420010054200120815

2209252208511

240945240636

26096260414

28098280102

30010030000

123

000

403032

806764

1208884

0551609896

2034200100100100

401024010085100

604280936998

80012343208049.898

1234000003606536.796

0100100100100200.160.160.260.224005325.296

2082.6867780400.3850.330.580.544403915.294

4063.868.35658600.710.650.890.9448027894

6044.949.14139801.081.081.171.38520170

803432.53126.41001.431.561.511.665607

100242122191201.71.971.832.126000

1201814161214022.32.22.5

1401391071602.4

16095631802.8

1806322200

2004100

22010

123

12312304.64.6

04.64.604.64.6104.564.38

104.564.38104.564.4204.354.01

204.354.01204.354.02304.113.77

304.113.77304.113.76403.93.533.53

403.93.533.53403.93.51503.673.25

503.673.3503.673.243.24603.433.08

603.433.08603.432.96703.172.8

703.172.8703.172.8802.962.61

802.962.58802.962.53902.732.39

902.73902.731002.532.23

1002.531002.531102.31

1102.311102.311202.11

1202.111202.111301.89

1301.821301.82140

140140

150

160

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

B

RR

1

2

3

t, s

% of total Sr2+

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

4

3

2

RR

A

1

2

3

4

t,

[Ca2+]o,%

0

0

0

0

0

t,

Ca2+,%

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s


000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s

ln A0/A

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

A

k=1,5.10-2 c-1

t1/2=46 c

1

2

3

4

t, s

[Ca2+]m, %

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

B

1

2

3

4

t,s

ln A0/A

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data: N6,7,85.07.05

1

2

3

t, c

ln C

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data:N2, 5.07.05

1

2

3

t, c

ln C

y1 = -0,278x + 4,8947R2 = 0,9953

y2 = -0,229x + 4,6R2 = 0,9899

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

t, c

ln C

00

00

00

1

2

1

2

1

2

1- 2- 2+

5

6

[Ca2+]o,

t1/2, c

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

RR

CsA

1

2

3

2+

1

2

3

t, c

[Ca2+]m, %

12121

010010004.64.603.27

109678.4104.564.38102.98

207855204.354.01202.22

306140304.113.7301.8

404931.4403.93.44401.53

503924503.673.17501.3

603118.9603.43360

702416703.172.8

801913802.962.58

90159902.732.32

1001371002.532.07

1101051102.31

120831202.11

130621301.9

14051140

15030150

1602160

1701

1800

190

200

1231231

0100100100010010010004

108074.3870.47207575.1868.73203.4

205556.0554.33406161.5657.96402.77

304342.6942.58605454.152.58602.2

403432.8233.5780515049.37801.8

502725.4126.541004947.747.241001.4

602219.7921.011204845.8245.72

701615.4816.651404645.2544.59

801312.1613.21604543.71

9099.5710.471804343.01

10077.58.3200

110566.6

12034.75.2

13023.74.1

140133.3

15002.42.6

1601.82.1

1701.51.6

1801.21.3

1900.91121234

20004.64.60100100100100

12204.014.252079.2555442.6

0100100403.53.724050.5343415.3

205570603.083.166028.722215.1

403441802.582.52801913132

6022241001.961.7910013781

8013131201208350

10075.5140513

12033160202

1401018001

1600200

180

1234

0

0.046

0.098

0.1270

0.150.049

0.1640.101

0.1750.138

0.1830.1590

0.1880.1730.027

0.1840.048

0.190.071

0.1930.0850

0.0940.01

0.0980.008

0.1020.011

0.1060.012

0.012

0.012

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t,

[Ca2+m], %

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

ln C

0

0

0

0

0

0

0

1

t, c

ln[C-C2exp(-k2t)]

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

B

1

2

3

t, c

[Ca2+m], %

000

000

000

000

000

000

000

000

000

000

000

1

2

3

t, c

[Ca2+m], %

0

0

0

0

0

0

1

t, c

ln (C-C1)

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

[Ca2+m], %

00

00

00

00

00

00

00

1

2

t, c

ln C

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

2

654,0,1 .

(1)

(2)

(1)

(2)

(1)

(2)

(3)

1

2

3

4

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

2

3

4

t, c

[Ca2+]m, %

4

28.925.2

1410

2.46.6

1

2

1

2

1

2

1- 2- 2+

5

6

[Ca2+]o,

t1/2, c

1

1234123123

05401004600000000

20322872402024405520204151

40851.5494140396675.540446383

60368322960508085.760637694

8008020.3198057.78791.7807379.498

1000871311.5100639096100788098.3

1209287.11206812081

123

0.025

123412340.005

120.3560.250.015

040400.2010.1850.025

2019.4220.10.12430.03

4017.6160.040.08930.035

0.010.06120.04

0.060.04080.045

0.1750.0530.02720.049

0.2350.0130.01460.051

0.2750.0190.00780.055

0.30.0390.00390.05

0.3150.0770.0070.160.025

0.3250.1240.040.02520.2250.005

0.330.1660.010.050.2650.013

0.3350.1920.060.0760.290.024

123450.340.2090.150.040.1050.0370.3050.031

0.110.1120.3430.220.1560.010.1250.0250.3150.038

0.1020.10560.2270.1590.060.150.0190.320.040.015

0.0560.1080.2280.1590.1450.170.0250.0420.01

0.030.09170.1520.190.050.060.015

0.0220.07880.1120.1530.2050.07280.0950.017

0.0220.06830.1040.1530.21750.0770.150.018

0.060.1080.22910.0770.1670.1850.017

0.0550.070.2330.0770.1160.20.015

0.0530.060.1120.240.0850.210.035

0.0510.0550.1080.2430.06210.2150.06

0.0520.1080.0470.2170.085

0.060.0370.105

0.0550.1120.030.12

0.05280.10640.0250.132

0.05130.10760.020.142

0.09710.0190.145

0.0760.01360.15

0.07460.0107

0.07460.0107

1

0000

0000

0000

0000

0000

0000

0000

t,

1

2

3

4

1

2

3

4

2+, %

2

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

A654, %

3

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

520, %

004455

00

00

t1/2,

1

2

1

2

2+

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

,

2+

520,0,05 .

2+

2+

2+

3

,

,

,

1

2

3

4

5

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

Ca2+

,

2+

3

654,0,1 .

1

2

3

4

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

Sr2+

,

1

2

3

Sr2+

654,0,1 .

3

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

2

3

A23187

A23187

A23187

1- CCCP,2- RR,3- 2+

3

654,0,1 .

1

2

3

1

1231234020401234123

000000000127.517.69.901001001001000000

20131212021.12016.7022413.282087798690200.340.140.09

4024221404231400326.510.8540765872.784400.690.30.2

6036341606138521430.5153.56064396072601.050.450.33

8047.3422807444521528.9142.48054.6264862801.430.620.45

100564221008346532625.2106.6100441736531001.770.810.62

120654331209048532120351027431202.20.980.75

14073433140934953414027721341402.691.160.93

160794441609654416021415261601.331.05

18084444180985441801621220

2008844420010054200120815

2209252208511

240945240636

26096260414

28098280102

30010030000

055

2034

4010

604

8001234

123400000

0100100100100200.160.160.260.22

2082.6867780400.3850.330.580.54

4063.868.35658600.710.650.890.94

6044.949.14139801.081.081.171.38

803432.53126.41001.431.561.511.66

100242122191201.71.971.832.12

1201814161214022.32.22.5

1401391071602.4

16095631802.8

1806322200

2004100

22010

123

12312304.64.6

04.64.604.64.6104.564.38

104.564.38104.564.4204.354.01

204.354.01204.354.02304.113.77

304.113.77304.113.76403.93.533.53

403.93.533.53403.93.51503.673.25

503.673.3503.673.243.24603.433.08

603.433.08603.432.96703.172.8

703.172.8703.172.8802.962.61

802.962.58802.962.53902.732.39

902.73902.731002.532.23

1002.531002.531102.31

1102.311102.311202.11

1202.111202.111301.89

1301.821301.82140

140140

150

160

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

B

RR

1

2

3

t, s

% of total Sr2+

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

4

3

2

RR

A

1

2

3

4

t,

[Ca2+]o,%

0

0

0

0

0

t,

Ca2+,%

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s


000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s

ln A0/A

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

A

k=1,5.10-2 c-1

t1/2=46 c

1

2

3

4

t, s

[Ca2+]m, %

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

B

1

2

3

4

t,s

ln A0/A

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data: N6,7,85.07.05

1

2

3

t, c

ln C

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data:N2, 5.07.05

1

2

3

t, c

ln C

y1 = -0,278x + 4,8947R2 = 0,9953

y2 = -0,229x + 4,6R2 = 0,9899

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

t, c

ln C

00

00

00

1

2

1

2

1

2

1- 2- 2+

5

6

[Ca2+]o,

t1/2, c

12121

010010004.64.603.27

109678.4104.564.38102.98

207855204.354.01202.22

306140304.113.7301.8

404931.4403.93.44401.53

503924503.673.17501.3

603118.9603.43360

702416703.172.8

801913802.962.58

90159902.732.32

1001371002.532.07

1101051102.31

120831202.11

130621301.9

14051140

15030150

1602160

1701

1800

190

200

1231231

0100100100010010010004

108074.3870.47207575.1868.73203.4

205556.0554.33406161.5657.96402.77

304342.6942.58605454.152.58602.2

403432.8233.5780515049.37801.8

502725.4126.541004947.747.241001.4

602219.7921.011204845.8245.72

701615.4816.651404645.2544.59

801312.1613.21604543.71

9099.5710.471804343.01

10077.58.3200

110566.6

12034.75.2

13023.74.1

140133.3

15002.42.6

1601.82.1

1701.51.6

1801.21.3

1900.91121234

20004.64.60100100100100

12204.014.252079.2555442.6

0100100403.53.724050.5343415.3

205570603.083.166028.722215.1

403441802.582.52801913132

6022241001.961.7910013781

8013131201208350

10075.5140513

12033160202

1401018001

1600200

180

1234

0

0.046

0.098

0.1270

0.150.049

0.1640.101

0.1750.138

0.1830.1590

0.1880.1730.027

0.1840.048

0.190.071

0.1930.0850

0.0940.01

0.0980.008

0.1020.011

0.1060.012

0.012

0.012

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t,

[Ca2+m], %

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

ln C

0

0

0

0

0

0

0

1

t, c

ln[C-C2exp(-k2t)]

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

B

1

2

3

t, c

[Ca2+m], %

000

000

000

000

000

000

000

000

000

000

000

1

2

3

t, c

[Ca2+m], %

0

0

0

0

0

0

1

t, c

ln (C-C1)

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

[Ca2+m], %

00

00

00

00

00

00

00

1

2

t, c

ln C

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

2

654,0,1 .

(1)

(2)

(1)

(2)

(1)

(2)

(3)

1

2

3

4

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

2

3

4

t, c

[Ca2+]m, %

1

100100100100

76555442.6

47.7343418.3

28.722217.3

1913132

13781

8350

513140

202160

01801180

200200200200

1

2

3

4

t, c

[Ca2+]m, %

1

1234123123

05401004600000000

20322872402024405520204151

40851.5494140396675.540446383

60368322960508085.760637694

8008020.3198057.78791.7807379.498

1000871311.5100639096100788098.3

1209287.11206812081

123

0.025

123412340.005

120.3560.250.015

040400.2010.1850.025

2019.4220.10.12430.03

4017.6160.040.08930.035

0.010.06120.04

0.060.04080.045

0.1750.0530.02720.049

0.2350.0130.01460.051

0.2750.0190.00780.055

0.30.0390.00390.05

0.3150.0770.0070.160.025

0.3250.1240.040.02520.2250.005

0.330.1660.010.050.2650.013

0.3350.1920.060.0760.290.024

123450.340.2090.150.040.1050.0370.3050.031

0.110.1120.3430.220.1560.010.1250.0250.3150.038

0.1020.10560.2270.1590.060.150.0190.320.040.015

0.0560.1080.2280.1590.1450.170.0250.0420.01

0.030.09170.1520.190.050.060.015

0.0220.07880.1120.1530.2050.07280.0950.017

0.0220.06830.1040.1530.21750.0770.150.018

0.060.1080.22910.0770.1670.1850.017

0.0550.070.2330.0770.1160.20.015

0.0530.060.1120.240.0850.210.035

0.0510.0550.1080.2430.06210.2150.06

0.0520.1080.0470.2170.085

0.060.0370.105

0.0550.1120.030.12

0.05280.10640.0250.132

0.05130.10760.020.142

0.09710.0190.145

0.0760.01360.15

0.07460.0107

0.07460.0107

1

0000

0000

0000

0000

0000

0000

0000

t,

1

2

3

4

1

2

3

4

2+, %

2

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

A654, %

3

000

000

000

000

000

000

000

t,

1

2

3

1

2

3

520, %

004455

00

00

t1/2,

1

2

1

2

2+

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

00000

,

2+

520,0,05 .

2+

2+

2+

3

,

,

,

1

2

3

4

5

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

Ca2+

,

2+

3

654,0,1 .

1

2

3

4

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

Sr2+

,

1

2

3

Sr2+

654,0,1 .

3

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1,2,3

A23187

A23187

1- CCCP,2- RR,3- 2+

3

654,0,1.

1

2

1,2

2+ 23187

1231234020401234123

000000000127.517.69.901001001001000000

20131212021.12016.7022413.282087798690200.340.140.09

4024221404231400326.510.8540765872.784400.690.30.2

6036341606138521430.5153.56064396072601.050.450.33

8047.3422807444521528.9142.48054.6264862801.430.620.45

100564221008346532625.2106.6100441736531001.770.810.62

120654331209048532120351027431202.20.980.75

14073433140934953414027721341402.691.160.93

160794441609654416021415261601.331.05

18084444180985441801621220

2008844420010054200120815

2209252208511

240945240636

26096260414

28098280102

30010030000

123

000

403032

806764

1208884

0551609896

2034200100100100

401024010085100

604280936998

80012343208049.898

1234000003606536.796

0100100100100200.160.160.260.224005325.296

2082.6867780400.3850.330.580.544403915.294

4063.868.35658600.710.650.890.9448027894

6044.949.14139801.081.081.171.38520170

803432.53126.41001.431.561.511.665607

100242122191201.71.971.832.126000

1201814161214022.32.22.5

1401391071602.4

16095631802.8

1806322200

2004100

22010

123

12312304.64.6

04.64.604.64.6104.564.38

104.564.38104.564.4204.354.01

204.354.01204.354.02304.113.77

304.113.77304.113.76403.93.533.53

403.93.533.53403.93.51503.673.25

503.673.3503.673.243.24603.433.08

603.433.08603.432.96703.172.8

703.172.8703.172.8802.962.61

802.962.58802.962.53902.732.39

902.73902.731002.532.23

1002.531002.531102.31

1102.311102.311202.11

1202.111202.111301.89

1301.821301.82140

140140

150

160

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

B

RR

1

2

3

t, s

% of total Sr2+

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

1

4

3

2

RR

A

1

2

3

4

t,

[Ca2+]o,%

0

0

0

0

0

t,

Ca2+,%

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s


000

000

000

000

000

000

000

000

000

1

2

3

1

2

3

t, s

ln A0/A

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

A

k=1,5.10-2 c-1

t1/2=46 c

1

2

3

4

t, s

[Ca2+]m, %

0000

0000

0000

0000

0000

0000

0000

0000

0000

0000

B

1

2

3

4

t,s

ln A0/A

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data: N6,7,85.07.05

1

2

3

t, c

ln C

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

data:N2, 5.07.05

1

2

3

t, c

ln C

y1 = -0,278x + 4,8947R2 = 0,9953

y2 = -0,229x + 4,6R2 = 0,9899

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

1

2

1

2

3

t, c

ln C

00

00

00

1

2

1

2

1

2

1- 2- 2+

5

6

[Ca2+]o,

t1/2, c

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

000

RR

CsA

1

2

3

2+

1

2

3

t, c

[Ca2+]m, %

12121

010010004.64.603.27

109678.4104.564.38102.98

207855204.354.01202.22

306140304.113.7301.8

404931.4403.93.44401.53

503924503.673.17501.3

603118.9603.43360

702416703.172.8

801913802.962.58

90159902.732.32

1001371002.532.07

1101051102.31

120831202.11

130621301.9

14051140

15030150

1602160

1701

1800

190

200

1231231

0100100100010010010004

108074.3870.47207575.1868.73203.4

205556.0554.33406161.5657.96402.77

304342.6942.58605454.152.58602.2

403432.8233.5780515049.37801.8

502725.4126.541004947.747.241001.4

602219.7921.011204845.8245.72

701615.4816.651404645.2544.59

801312.1613.21604543.71

9099.5710.471804343.01

10077.58.3200

110566.6

12034.75.2

13023.74.1

140133.3

15002.42.6

1601.82.1

1701.51.6

1801.21.3

1900.91121234

20004.64.60100100100100

12204.014.252076555442.6

0100100403.53.724047.7343418.3

205570603.083.166028.722217.3

403441802.582.52801913132

6022241001.961.7910013781

8013131201208350

10075.5140513

12033160202

1401018001

1600200

180

1234

0

0.046

0.098

0.1270

0.150.049

0.1640.101

0.1750.138

0.1830.1590

0.1880.1730.027

0.1840.048

0.190.071

0.1930.0850

0.0940.01

0.0980.008

0.1020.011

0.1060.012

0.012

0.012

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t,

[Ca2+m], %

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

00

1

2

1

2

t, c

ln C

0

0

0

0

0

0

0

1

t, c

ln[C-C2e

Documents

В.Ф.Сагач Інститут фізіології ім. О.О.Богомольця НАН України