EXTRACELLULAR MATRIX ECM

ECM ECM

ECM(extracellular matrix)ECMECMECM

MACROMOLECULAR ORGANIZATION OF ECM

ECM

Collagen(collagen)

19IIIIIIVXI30

Collagen

collagenstructure (tropocollagen)(Gly-x-y)n x(Pro) y(Hypro)(Hylys)- 300nm 1.5nm

Structures of the collagen67nm

TEM image of collagen

;

RERProLys43CVC

(collagen fibril)

Collagen fibrils around a fibroblast??

1890""

FibronectinFNFN VFNECMFN204.59.5FN57

Fibronectin helps connect the inside of the cell to the outside

fibronectin dimer

FN RGD: FN Arg-Gly-Asp

FNFNFNFNECMFNRGDArg-Gly-Asp FNRGDFNFN

laminin(LN)7LN81-31-31-2FN815-2850N-

Laminin Structure

12

50nm200nm

Basal lamina: ; ; (Schwann cells)

()

: LN :

1glycosaminoglycanGAGGAG(HA)GAG10

N-

0

N-

0.2~2.3

N-

1.0~2.0

N-

0.2~3.0

N-

2.0~3.0

N-

0.9~1.8

2 proteoglycanHASerGAGXyl-Gal-Gal-GlcUASerHAGAGHA

A Proteoglycan Complex.Galactose N-Acetyl-D-glucosamineD-glucuronic acid N-Acetyl-D-galactosamine

:

-

--(fiber-network)-

elastin AlaLys

Elastin

1ECManoikis2

3anchorage dependent growth45

:

Cell membranePlasma membraneInternal membraneBiomembraneunit membrane lipid raftcaveolae liposomedetergentghostmembrane associated cytoskeletoncell junctiontight junctionanchoring junctionsdesmosomegap junctioncell adhesion moleculeCAMcell adhensioncadherinintegrin Cell coatglacocalyxextracellular matrixECMbasal membraneglycosaminoglycanGAG

1 2 -------- 3 4 5___________________________________________________________________

1 A B3 C D72 A B C D3 A B C D4 ABCD5 A B C D6L- A B C D7 A B C D

162Gly-x-y3 4 5RGD