----

1.2.SLESLE3.ANA4.ENA5.dsDNA6.RF7.8.9.

1.Ag

Ag

2.T 70

3.

26-1

(Graves)

TSH

(Addison)

IgG

26-1

(Graves)

TSH

(Addison)

IgG

4.AID AID

5. AID

IIIIIT

6. AIDIIAID

1autoimmune hemolytic anemia, AIHA + + Coombs test

2immunologic thrombocytopenic purpura, ITPplt,1/10000plt

()SLERA T IDM1 MS

1SLE,Ig-

Sm dsDNA DNA ssDNA 10%15%SLE

75%80% 10%60% Coombs RF30%

2RA RFTNF1RF23

ANAIgGIgAIgMCH50C3RFENAdsDNA7. AID

95%99%SLE SLE IC RASLE

ENA dsDNASmrib ANA

1IgIgGIgMIgAIg2 AIDTAID

8.HEp-2

indirect immunofluorescence, IIF

ELISA Western-blot 9.

FPIA TR-FIA10.

11.

1 ------ ------

2

11.

1

2

3

4

Ag cell 1 2 3TB

DNA dsDNADNA dsDNASLE

12. DNA

5

1~21~22~3

ENA: ENA Sm U1-RNP SSA SSB Jo-1 Scl-70 rib-P13. ENA

SDS-PAGE

1SmSLE SmUUsnRNP29KD28KD13.5KDSLESmSnRNPSmU1RNPU1RNP

2SSARoSSARoscRNPsYYRNA52KD60KD52KDSS60KDSLESSA6075SSA

3SSB/La/HasnRNPRNARNA 48KD47KD45KD48KDSSBSSASSBSSA40SSB

4Scl-70100KDDNAIPSSScl-7070KDScl-70PSS30%40%PSSSSC100%Scl-70

5JO-1 Jo-1tRNAscRNPs50KDtRNAJo-1PMPM25DM7.1%PMDM6067Jo-1HLADR3

6rRNPribosomerRNPrRNP38KD 16KD15KDrRNPSLESLE10%20%SLErRNPSLEANArRNPrRNPdsDNAdsDNA12

7RNPnRNPnuclear RNPU1RNPU1snRNPU1RNP970KD32KD22KD U1U1snRNPU1RNP

1(MCTD)MCTDRNPMCTD2SLERNPSLE40%RNPSLEDNARNPRNPds-DNASm3RNP

rheumatoid factor, RFIgG FcIgM RFIgG 14. RF

1 IgM-RF 2ARRAYIMAGE IgM-RF 3ELISA RF

1RFRASLEPSS

%

RA79SLE30SS95Sclerosis80PM80MCTD25

2 RFRA IgM-RF>80IU/ml 3IgG-RFRA IgM-RF RA 4IgA-RFRA

16313373815kgT:37.8P100/R20/BP120/90mmHg IgG28.3g/lIgA3.40g/lIgM3.02g/lC30.43g/lC40.06g/l MA:14mg/LTRF:1.5mg/LIgG:9.5mg/L1M:10mg/L

SLESLESLE

SLEdsDNASmSLE RFANAdsDNAENACRPCICSLE ANARFIgGMAdsDNAENA

ANARFENAdsDNA

1.2.

ANA

ANA1. 4~8NS20min2. 1cm3. PBS1:1050ul3730min4. ddH2OPBS1235min5. FITC-IgG10ul3730min6. 47.

1.2.3. 1. 2. 3.MLR 4.HLA 5.FCS

HLACDCFSSS

*Complement mediated lysis