140-0002 2-2-24 4

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DNARNA

................................................................................................................................2

TRIS-HCL .........................................................................2

PBS(-).......................................................................................................................................................................3

5N ....................................................................................................................................................4

EDTA...................................................................................................................................................................4

TE (PH 8.0) ..........................................................................................................................................4

- .......................................................................................................5

-....................................................................................................5

.............................................................................................................................................6

RNASE..................................................................................................................................................................7

70% .........................................................................................................................................................7

3 M (PH 5.2) .................................................................................................................................8

TAE--EDTA ....................................................................................................8

TBE--EDTA.................................................................................................8

........................................................................................................................................9

............................................................................................................................9

DNA ..................................................................................................... 10

DEPC ........................................................................................................................................................... 11

....................................................................................................................................................12

-SDS ............................................................................ 12

......................................................................................................................... 15

..................................................................................................................................................................... 16

.............................................................................................................................................. 16

DNA.................................................................................................................................... 17

DNA........................................................................................................................................................................... 17

.................................................................................................................................................... 17

................................................................................................... 18

PEG.......................................................................................................................... 19

RNA .............................................................................................................................................. 19

............................................................................................................................ 20

.................................................................................................... 20

2

Tris-HCl

Tris; tris(hydroxymethyl)- aminomethanepH 79Trizma

Tris base; Trizma Base pH

1. 12.1 g Trizma Base 80 mL

2. pH pH pH

pH

3. pH 100 mL

pH Trizma Base

(Trizma HCl)

Trizma Base pH 10.4Trizma HCl

pH 4.7

pH

0.05 M 7.6 6.06 g Trizma HCl 1.39 g Trizma Base 1L

0.05 M 7.8 5.32 g Trizma HCl 1.97 g Trizma Base 1L

0.05 M 8.0 4.44 g Trizma HCl 2.65 g Trizma Base 1L pH Trizma Trizma HCl Trizma Base pH

pH Tris Trizma Pre-set crystals

Trizma Pre-set crystals Trizma Base Trizma HCl

pH

1M, 100 mL

(Trizma base) 12.1 g T6791

10 mL H1758

(Trizma HCl) T5941

(Trizma base) T6791

pH

Trizma Pre-set crystals T7693 pH 7.4

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3

PBS(-)

PBS (Phosphate Buffered Saline; ) PBS PBS(-)PBS PBSDulbeccos PBS; D-PBS

PBS- PBS PBS(+)

PBS 1x, 1 L 10x, 1 L

0.2 g 2 g P5655

0.2 g 2 g P9333

1.15 g 11.5 g S7907

12 2.90 g 29.0 g 28-3720

8 g 80 g S7653

PBS(+) (0.133 g) (1.33 g) C5080

PBS(+) (0.1 g) (1 g) M2393

1xPBS(-), 1L

1NaClNa2HPO4KClKH2PO4 800 mL 1L pH 7.4 1xPBS

1.15 g 2.90 g 12 28-3720

10xPBS(-), 1L

10NaClNa2HPO4KClKH2PO4 1L pH 7.4 10xPBS

11.5 g 29.0 g 12 28-3720

10PBS 100 mL 10PBS 900 mL

PBS(+)0.133 g (CaCl22H2O) 0.1 g

MgCl26H2O

PBS(+) PBS(-)

PBS- D8537 PBS-10 D1408

PBS+ D8662 PBS+10 D1283

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4

EDTA

(EDTA2Na2H2O) 372.24

E5134

NaOH 40.00

S8045 045

5N 5N

pH pH

100 mL

100 mL

1. 70 mL 20.0 g

2. 100 mL

EDTA

EDTA 110 M

0.5 M pH 8.0100 mL 1. 18.6 g EDTA EDTA2Na2H2O 80 mL 2. 1 pH pH 8.0 3. pH 8.0 5N pH 8.0 4. 100 mL

2-8

TE (pH 8.0)

TE Tris-EDTA Buffer; -EDTA DNA 10 mM Tris, 1mM EDTApH 8.0

TE 100 mL

, pH 8.0, 1 M 1 mL T2694

EDTA , 0.5 M 0.2 mL

1. 1 M Tris pH 8.01.0 mL 0.5 M EDTApH 8.00.2 mL 100 mL 2. 121 15

2-8

100TE T9285

1 M Tris, 100 mM EDTA (pH 8.0)

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5

-

DNA pH 8.0

RNA

8- 100 mg H6878

100 mL T2694(1 M)

100 g P1037

1. 100 g 65 2. 8- 100 mg 0.1%

8-8-

3. 100 mL 0.5 M pH 8.0 15

4. 2 5. pH pH 6. pH 7.8 35 7. 0.1 M pH 8.02

2-8-20 0.1 M

-

1. 2. 65 3. 4. 2 2-8-20

(DNA) P4557

(RNA) P4682

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