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魏炽炬
Epithelial–Mesenchymal Transition
Origin of Epithelium and Mesenchyme
• There are two major types of cell arrangements in the embryo:
• epithelial cells, which are tightly connected to one another in sheets or tubes,
• and mesenchymal cells, which are unconnected to one other and operate as independent units.
Origin of Epithelium and Mesenchyme
• Epithelia are sheets or tubes of connected cells; they can originate from any germ layer.
• Meschymal cells are loosely packed unconnected cells; mesenchyme contains collagen bundles and fibroblasts; they are derived from the mesoderm or neural crest.
In the Adult
• Epithelium is a tissue composed of cells that line the cavities and surfaces of structures throughout the body.
• Epithelium is classified as a primary body tissue, the other ones being connective tissue, muscle tissue and nervous tissue.
In the Adult
• Mesenchyme later differentiates into blood vessels, blood-related organs, and connective tissues.
• All organs consist of an epithelium and an associated mesenchyme.
Epithelial–Mesenchymal Transition
Hartmut Beug
Actin/
DAPI
E-cadherin
Vimentin
Desmosomes
Vimentin
Epithelial
Mesenchymal
Epithelial–Mesenchymal TransitionJean Paul Thiery
EMT in Tissue RemodellingJean Paul Thiery
The generation of three germ layers
下胚层
The migration of neural crest
Somitogenesis
The generation of glomerulus
EMT During Tumor ProgressionHartmut Beug
Paper One
Epithelial-to Mesenchymal Transition Generates Proliferative Human Islet Prec
ursor Cells
• Marvin Gershengorn, Anandwardhan Hardikar, Chiju Wei, Elizabeth Geras-Raaka, Bernice Marcus- Samuels, Bruce M. Raaka• SCIENCE VOL 306, 2004
Embryonic StemEmbryonic StemCellCell
Bone MarrowBone MarrowStem CellStem Cell
TransdifferentiationTransdifferentiation
Pancreatic stem Pancreatic stem cellcell
Cadaveric hCadaveric human isletsuman islets
Potential Sources of Islet Precursor CellsPotential Sources of Islet Precursor Cells
hIPC Generation and ICA Formation hIPC Generation and ICA Formation
A B C
1 hr 24 hr 6 day
D E F
Transition From Insulin+ to VimentiTransition From Insulin+ to Vimentin+ Cells n+ Cells
Redifferentiation From Vimentin+ to Redifferentiation From Vimentin+ to Insulin+ Cells Insulin+ Cells
Islet Precursors Generated by EMT Islet Precursors Generated by EMT Marvin Gershengorn
EMT in Progenitor Cells of The Developing Endocrine Pancreas
Joel F. Habener
EpitheliumEpithelium
Basel membraneBasel membrane
ECMECM
Acinar cellAcinar cell
Islet
Paper Two
Islet-Derived Fibroblast-Like Cells Are Not Derived via Epithelial-Mesenchymal Transition From Pdx-1 or Insulin-Positive
Cells
• Lucas G. Chase, Fernando Ulloa-Montoya, Benjamin L. Kidder, and Catherine M. Verfaillie• Diabetes 56:3–7, 2007
Lineage Tracing on Proliferative Fibroblast-like Cell Populations
Fibroblast-like Cells Are Not Generated From Insulin+/PDX1+ Cells
About 25% of All Insulin-Expressing Cells Coexpressed EGFP
Nadya L. Lumelsky
Paper Three
In Vitro Proliferation of Cells Derived From Adult Human -Cells Revealed By Cell-
Lineage Tracing
• Holger A. Russ, Yael Bar, Philippe Ravassard, and Shimon Efrat• Diabetes 57, 2008
In Vitro Lineage Tracing of Islet Beta Cells
Labeling of human islet cells with the two-virus system