-Lampiran 1 : Surat Persetujuan Kelayakan Komite Etik

164

-Lampiran 1 : Surat Persetujuan Kelayakan Komite Etik

Universitas Sumatera Utara

165

Lampiran 2 : Surat Ijin Melakukan Penelitian


166

Lampiran 3 : Surat Balasan Ijin melakukan penelitian


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Lampiran 4 : Surat Keterangan Selesai Melakukan Penelitian


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Lampiran 5 : Surat Keterangan Presentasi Penelitian


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Lampiran 6 : Surat Keterangan Accepted/Published Journal International


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Lampiran 7 : Konversi Dosis Manusia – Hewan Lawrence & Bacharach

Tabel 1. Penentuan Konversi Dosis Manusia dan Hewan Coba

Mencit 20 gr

Tikus 200 gr

Marmot 400 gr

Kelinci 1.5 kg

Kucing 2 kg

Kera 12 kg

Anjing 12 Kg

Manusia 70 kg

Mencit 20 gr 1.00 7.00 12.29 27.80 23.70 64.10 124.2 387.9

Tikus 200 gr 0.14 1.00 1.74 3.30 4.20 9.20 17.80 56.00

Marmot 400 gr 0.08 0.57 1.00 2.25 2.40 5.20 10.20 31.50

Kelinci 1.5 kg 0.04 0.25 0.44 1.00 1.08 2.40 4.50 14.20

Kucing 2 kg 0.03 0.23 0.41 0.92 1.00 2.20 4.10 13.00

Kera 12 kg 0.016 0.11 0.19 0.42 0.45 1.00 1.90 6.10

Anjing 12 Kg 0.008 0.06 0.10 0.22 0.24 0.52 1.00 3.10

Manusia 70 kg 0.0026 0.018 0.31 0.07 0.13 0.16 0.32 1.00

(Suhardjono D, 1995. Percobaan Hewan Laboratorium, Yogyakarta : Gajah Mada University Press, hal. 207)

Tabel 2. Daftar volume maksimal larutan sediaan uji yang dapat diberikan pada berbagai hewan

(Suhardjono D, 1995. Percobaan Hewan Laboratorium, Yogyakarta : Gajah Mada University Press, hal. 207)

i.v : intravena i.m. : intramuscular i.p. : intraperitoneal s.c. : subcutan p.o : peroral


171

DATA ELISA TNF-α

02.365 2001.332 1000.532 500.203 250.096 12.5

1.365 1.659 2.659 0.036 0.12 0.158 0.256 0.598 2.458 0.265 0.548 2.548 0.168 0.268 2.0981.598 2.021 2.036 0.098 0.154 0.086 0.223 0.958 2.486 0.226 0.659 1.985 0.155 0.269 1.9891.229 2.124 2.459 0.099 0.158 0.145 0.249 0.659 2.487 0.198 0.254 2.489 0.261 0.325 2.0551.643 1.978 2.159 0.101 0.097 0.184 0.228 0.598 2.54 0.426 0.578 2.158 0.112 0.265 2.3691.556 1.597 2.686 0.076 0.108 0.245 0.198 0.597 2.568 0.209 0.689 2.489 0.095 0.226 2.5691.026 2.526 3.024 0.086 0.135 0.184 0.326 0.865 2.189 0.154 0.752 2.158 0.122 0.321 2.587

s-18 s-11 s-4 k-18 k-11 k-4 ST-18 ST-11 ST-4 SGH-18 SGH-11 SGH-4 SGHT-18 SGHT-11 SGHT-41 105.653 129.363 210.008 1.524 5.250 8.315 16.218 43.798 193.798 16.944 39.766 201.056 9.121 17.185 164.7662 124.444 158.556 159.766 3.476 7.992 2.508 13.556 72.831 196.056 13.798 48.718 155.653 8.073 17.266 155.9763 94.685 166.863 193.879 3.556 8.315 7.266 15.653 48.718 196.137 11.540 16.056 196.298 16.621 21.782 161.2984 128.073 155.089 169.685 3.718 3.395 10.411 13.960 43.798 200.411 29.927 42.185 169.605 4.605 16.944 186.6215 121.056 124.363 212.185 1.702 4.282 15.331 11.540 43.718 202.669 12.427 51.137 196.298 3.234 13.798 202.7506 78.315 199.282 239.444 2.508 6.460 10.411 21.863 65.331 172.105 7.992 56.218 169.605 5.411 21.460 204.202

KELOMPOKHASIL TNF-a

y = 0.012x - 0.054R² = 0.991

00.5

11.5

22.5

3

0 50 100 150 200 250

Lampiran 8 : Data Hasil Penelitian


172

DATA ELISA IL-6

02.365 2001.659 1000.659 500.265 250.125 12.5

0.698 1.998 2.269 0.125 0.098 0.098 0.158 0.562 2.025 0.102 0.215 2.262 0.125 0.22 2.2550.968 2.026 2.659 0.12 0.122 0.108 0.223 0.512 2.256 0.232 0.265 2.158 0.23 0.214 2.3640.965 2.215 2.158 0.098 0.101 0.105 0.215 0.354 2.65 0.265 0.223 2.258 0.154 0.236 2.5481.256 1.965 2.369 0.12 0.105 0.125 0.109 0.522 2.154 0.158 0.258 2.658 0.128 0.214 2.6580.659 1.996 2.658 0.089 0.212 0.091 0.158 0.625 2.265 0.256 0.226 2.365 0.214 0.298 2.5690.751 2.069 2.158 0.086 0.102 0.11 0.125 0.552 2.154 0.154 0.325 2.369 0.11 0.236 2.158

s-18 s-11 s-4 k-18 k-11 k-4 ST-18 ST-11 ST-4 SGH-18 SGH-11 SGH-4 SGHT-18 SGHT-11 SGHT-41 52.008 157.699 179.732 5.423 3.228 3.228 8.106 40.951 159.894 3.553 12.740 179.163 5.423 13.146 178.5932 73.959 159.976 211.439 5.016 5.179 4.041 13.390 36.886 178.675 14.122 16.805 170.707 13.959 12.659 187.4553 73.715 175.341 170.707 3.228 3.472 3.797 12.740 24.041 210.707 16.805 13.390 178.837 7.780 14.447 202.4154 97.374 155.016 187.862 5.016 3.797 5.423 4.122 37.699 170.382 8.106 16.236 211.358 5.667 12.659 211.3585 48.837 157.537 211.358 2.496 12.496 2.659 8.106 46.073 179.407 16.073 13.634 187.537 12.659 19.488 204.1226 56.317 163.472 170.707 2.252 3.553 4.203 5.423 40.138 170.382 7.780 21.683 187.862 4.203 14.447 170.707

AHASIL IL6

D E C B

y = 0.012x + 0.058R² = 0.951

0

0.5

1

1.5

2

2.5

3

0 50 100 150 200 250


173

DATA PENELITIAN Nilai mTOR tikus

SGHT SGH ST S K

4

43.918 42.798 40.515 35.453 41.300 40.575 39.416 36.760 28.175 37.385 43.301 41.800 38.961 34.067 39.184 72.756 53.787 40.697 38.687 41.696 42.407 39.307 40.589 29.711 35.700 61.194 47.002 50.972 40.194 43.002

11

91.009 90.970 70.300 20.910 38.000 92.970 86.850 68.900 20.970 62.898 84.000 80.971 56.100 27.060 34.360 60.110 33.141 31.110 11.111 29.150 68.991 62.989 50.304 24.980 40.000 62.996 46.804 36.794 25.774 35.107

18

105.061 103.760 82.991 25.060 53.000 80.567 78.862 65.796 17.907 55.868 88.020 76.011 53.111 29.081 43.080 80.071 79.066 59.986 20.111 40.976 93.001 91.000 63.911 33.027 43.000 93.000 88.925 69.940 20.001 44.000


174

DATA PENELITIAN Nilai p70S6K tikus

SGHT SGH ST S K

4

10.812 8.653 8.584 3.585 6.871 15.783 12.287 10.212 6.337 9.198 16.188 10.988 9.106 3.374 6.222 18.860 17.893 9.980 4.802 6.980 17.105 14.108 10.090 4.233 7.963

9.018 8.827 8.144 1.237 3.641

11

16.453 14.761 11.368 10.492 11.189 19.205 18.894 14.220 8.188 12.891 18.789 18.795 16.008 8.111 12.998 20.304 18.315 15.305 7.306 10.193 17.271 15.276 10.909 8.161 12.215 13.923 13.144 9.918 5.219 8.808

18

23.978 20.725 19.096 9.749 17.753 25.002 22.118 20.789 7.115 16.900 25.890 23.896 20.889 6.669 18.088 28.676 25.424 12.313 4.404 10.302 21.291 18.499 11.468 7.285 12.290 24.019 20.019 9.019 3.019 9.014


175

DATA ELISA PREALBUMIN 0

0.5 500 0.2 250 0.1 125

0 63 0 31

OD (450/630 nm)

Konsen trasi (ng/mL)

INKU BASI KElLOMPOK

0.6325 641.5

585.37

18 GH+T a3 0.6214 630.4 a3 0.4369 445.9 a3 0.659 668 a3

0.5563 565.3 a3 0.5521 561.1 a3 0.4689 477.9

386.88

11 a2 0.3598 368.8 a2 0.4215 430.5 a2 0.2659 274.9 a2 0.3256 334.6 a2 0.4256 434.6 a2 0.2333 242.3

218.15

4 a1 0.2154 224.4 a1 0.1065 115.5 a1 0.265 274 a1

0.2658 274.8 a1 0.1689 177.9 a1


176

0.5689 577.9

565.45

18 GH b3 0.6485 657.5 b3 0.4598 468.8 b3 0.5469 555.9 b3 0.4598 468.8 b3 0.6548 663.8 b3 0.3423 351.3

425.95

11 b2 0.4567 465.7 b2 0.5648 573.8 b2 0.4325 441.5 b2 0.3256 334.6 b2 0.3798 388.8 b2 0.4125 421.5

343.48

4 b1 0.2315 240.5 b1 0.2369 245.9 b1 0.3569 365.9 b1 0.4123 421.3 b1 0.3568 365.8 b1 0.2315 240.5

251.88

18 T c3 0.1256 134.6 c3 0.2358 244.8 c3 0.2365 245.5 c3 0.4123 421.3 c3 0.2156 224.6 c3 0.1233 132.3

165.72

11 c2 0.2156 224.6 c2 0.1546 163.6 c2 0.1256 134.6 c2 0.1156 124.6 c2 0.2056 214.6 c2 0.1068 115.8

119.15

4 c1 0.1054 114.4 c1 0.1103 119.3 c1 0.1125 121.5 c1 0.1023 111.3 c1 0.1236 132.6 c1 0.0932 102.2

85.57

18 S d3 0.0884 97.4 d3 0.0815 90.5 d3 0.0587 67.7 d3 0.0689 77.9 d3 0.0687 77.7 d3


177

0.0369 45.9

52.85

11 d2 0.0459 54.9 d2 0.0556 64.6 d2 0.0326 41.6 d2 0.0258 34.8 d2 0.0663 75.3 d2 0.0359 44.9

27.17

4 d1 0.0126 21.6 d1 0.0201 29.1 d1 0.0135 22.5 d1 0.0113 20.3 d1 0.0156 24.6 d1 0.2356 244.6

252.97

18 Kontrol e3 0.2156 224.6 e3 0.2356 244.6 e3 0.2141 223.1 e3

0.326 335 e3 0.2369 245.9 e3

0.215 224

273.37

11 e2 0.3458 354.8 e2 0.2158 224.8 e2 0.3269 335.9 e2 0.2458 254.8 e2 0.2369 245.9 e2

0.154 163

212.68

4 e1 0.1259 134.9 e1 0.2365 245.5 e1 0.2158 224.8 e1

0.321 330 e1 0.1689 177.9 e1


178

Lampiran 9 : Gambar Pelaksanaan Penelitian

Gambar 1. Kandang kosong tempat pemeliharaan tikus (6 ekor tikus tiap kandang/kelompok)

Gambar 2. Kandang tempat pemeliharaan tikus yang berisi tikus dilengkapi minuman dan pakan yang cukup.


179

Gambar 3. Alat , Bahan dan Prosedur Pembuatan Larutan Suspensi

30 % Cecal Inoculum

Gambar 4 . Tatalaksana pengambilan darah Intra Vena pada tikus

untuk pemeriksaan penanda yang diperlukan.


180

Gambar 5 . Prosedur induksi sepsis dengan penyuntikan suspensi

cecal inoculum intra peritoneal (i.p.)


Documents

-Lampiran 1 : Surat Persetujuan Kelayakan Komite Etik