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Supplemental figure. A. Wt b 3-2 b 3-3 b 3-4 b 3-5 b 3-6 mock mock mock mock. B. NP-9 3 -3. NP-9 3 -4. NP-9 3 -5. NP-9 3 -6. v 3 (FITC) and F-actin (TR). Supplemental figure. NP-9 β 3 clones: expression of β 3 and cell morphology. - PowerPoint PPT Presentation
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A
v3 (FITC) and F-actin (TR)
3
NP-18 NP-9
M21
WT
3-1
moc
kW
T
3-2
3-3
3-4
3-5
3-6
moc
k
moc
k
moc
k
moc
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3
NP-18 NP-9
M21
WT
3-1
moc
kW
T
3-2
3-3
3-4
3-5
3-6
moc
k
moc
k
moc
k
moc
kNP-93-3 NP-93-4 NP-93-5 NP-93-6
Supplemental figure. NP-9β3 clones: expression of β3 and cell morphology.A. Different clones for NP-9β3 transfected cells were grown in complete medium. After 24 hours, 40 g of total protein extracts were immunoblotted using antibody against β3: β3(AP3). wt: parental, mock: mock-transfected and β3: β3-transfected. B. Cells were seeded on glass coverslips, fixed, permeabilized and stained for: αvβ3 integrin (FITC) and F-actin (TR). All samples were analyzed using confocal microscopy. Bars, 20 m.
Wt 3-2 3-3 3-4 3-5 3-6 mock mock mock mock
Supplemental figure
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