9
!"#$%&2019 39 ): 1212-1220 犃犮狋犪犅狅狋 犅狅狉犲犪犾 犗犮犮犻犱犲狀狋 犛犻狀 犱狅犻 10.7606 j.issn.10004025.2019.07.1212 http :// xbzwxb.allj ournal.net !"#$20190411 %&"!'#$20190717 ()*+'()*+,-. 2018YFD1000700 2018YFD1000705 ); '(/01%2331461143017 ); 456789:;<=>?@ 11182130109236104 ); $767ABCDE?@ 111721301354052008 ); '(FG$7HABIJKLMN9IJNICGR062 ,-./OPQ1993- ), RSTUV+WX+WYZ[G$\]^7Email 651628238 qq.com _`Gabcd+WefVghijklMN7Hmno2pq%Email caoqinghe jaas.ac.cn 01234567 犛犛犚 859:;<=>? !"# $%& '() * + , - ./0 r'Fs1%t MN+WuvwKxyzKLFs1%+WuvwKL 221131 {|}% ~%t{ 570228 @ A+W2 TPM13SSR 895r'<45 99MN67[ SSR 5=456701 :;<g DPS-67\]  MEGA ¡¢£¤ ¥¦§8¨© Structureª«¬-®¤¥\]¯°8¨±²³67\]°´µ67¶·¸ ¹º»r'MN4567S DNA ¼I½¾¿[MN67ÀÁ¶ÂÃÄÅ67ÆÇÈÉÊË̯Íλ28®Ï$ÐÑÒÓ162ÔÕÖ×ØÙ[96.30% Ú®Ï$IÛÜÝ5.57Ô×ØÞÕÖ。( °=ß99M N4567\]¤àá9967\]S 0.4646IÛ\][ 0.3077 S\][ 0. 2670âã99678[ä§åæçXØNz67èéêç¶Â67ëìí¦SçîïXØ Nz67S³§år½®8ð8ñ。( 67\]2òóôõ8XØNz67½¾Þóöå÷¯°8 ¨ã8[äøåo¦§8¨8å¯Í2Âçùúr31ûꪫüB\]ýþó[ÿBCDMN4567SSR \]×Þ 0E>FGQ346 .5 Q789 HI8JK犌犲狀犲狋犻犮犇犻狏犲狉狊犻狋 狅犳狋犺犲犚犲 犻狊狋犲狉犲犱犛狑犲犲狋 狅狋犪狋狅犞犪狉犻犲狋犻犲狊 犻狀犆犺犻狀犪犫 犛犛犚 犕犪狉犽犲狉狊 ZHAOLukuan SUYijun DAIXibin WANGYao YUANRui CAOQinghe 1SweetpotatoResearchInstitute ChinaAgriculturalAcademyofSciences JiangsuXuhuaiRegionalAgriculturalAcademyof Sciences Xuzhou Jiangsu221131 China 2CollegeofHorticulture UniversityofHainan Haikou570228 China 犃犫狊狋狉犪犮狋 BasedonTPM13SSRfluorescentcapillarydetectiontechnology weanalyzedthegeneticdiver sityof99registeredsweetpotatovarieties.The 01 databaseoftheregisteredvarietieswasestablishedby SSRmarkers.TheNeigeneticdistanceamongdifferentvarietieswascalculatedusingtheDPSsoftware. TheNeighborJoiningmethodinMEGA wasusedforclusteranalysis andthegeneticstructureoftheac cessionswasanalyzedbytheadmixtureancestrymodelinStructuresoftware.Thegeneticcompositionof thevarietiesandthegeneticrelationshipamongthevarietieswerediscussed whichrevealedthesimilarity ofthesweetpotatovarietiesattheDNAlevelandprovidedreferencesforvarietyevaluation excellentpar entselectionandvarietyimprovement.Theresultsshowedthat :( atotalof162reproduciblebands wereamplifiedfrom28primerpairswithanaverageof5.57bandsandpolymorphicratewas96.30%. Thedendrogramof99registeredvarietieswasconstructed.Andthegeneticdistanceof99varietiesranged from0to0.4646withanaverageof0.3077.Clusteranalysisat0.2670geneticdistancecandivide99va

01234567犛犛犚 859:;?

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书书书

!"#$%&

,2019,39(7):1212-1220犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.

  犱狅犻:10.7606/j.issn.10004025.2019.07.1212                   http://xbzwxb.alljournal.net

!"#$

:20190411;%&"!'#$

:20190717

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(11182130109236104);$767ABCDE?@

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(NICGR062)

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。Email:651628238@qq.com

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。Email:caoqinghe@jaas.ac.cn

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犌犲狀犲狋犻犮犇犻狏犲狉狊犻狋狔狅犳狋犺犲犚犲犵犻狊狋犲狉犲犱犛狑犲犲狋狆狅狋犪狋狅犞犪狉犻犲狋犻犲狊

犻狀犆犺犻狀犪犫狔犛犛犚犕犪狉犽犲狉狊

ZHAOLukuan1,SUYijun1,DAIXibin1,WANGYao2,YUANRui1,CAOQinghe

(1SweetpotatoResearchInstitute,ChinaAgriculturalAcademyofSciences,JiangsuXuhuaiRegionalAgriculturalAcademyof

Sciences,Xuzhou,Jiangsu221131,China;2CollegeofHorticulture,UniversityofHainan,Haikou570228,China)

犃犫狊狋狉犪犮狋:BasedonTPM13SSRfluorescentcapillarydetectiontechnology,weanalyzedthegeneticdiver

sityof99registeredsweetpotatovarieties.The“01”databaseoftheregisteredvarietieswasestablishedby

SSRmarkers.TheNeigeneticdistanceamongdifferentvarietieswascalculatedusingtheDPSsoftware.

TheNeighborJoiningmethodinMEGAwasusedforclusteranalysis,andthegeneticstructureoftheac

cessionswasanalyzedbytheadmixtureancestrymodelinStructuresoftware.Thegeneticcompositionof

thevarietiesandthegeneticrelationshipamongthevarietieswerediscussed,whichrevealedthesimilarity

ofthesweetpotatovarietiesattheDNAlevelandprovidedreferencesforvarietyevaluation,excellentpar

entselectionandvarietyimprovement.Theresultsshowedthat:(1)atotalof162reproduciblebands

wereamplifiedfrom28primerpairswithanaverageof5.57bandsandpolymorphicratewas96.30%.(2)

Thedendrogramof99registeredvarietieswasconstructed.Andthegeneticdistanceof99varietiesranged

from0to0.4646withanaverageof0.3077.Clusteranalysisat0.2670geneticdistancecandivide99va

rietiesintothreegroups.Sweetpotatovarietiesfromthesameagroecologicalzonesorcloserelationship

wereclusteredinthesamegroups.Thevarietiesfromdifferentagroecologicalzoneswererelativelyscat

teredindifferentgroups.(3)Thegeneticbackgroundamongaccessionsisrelativelynarrow.Andsimilari

tyofsomevarietiesishigherinsomecertainoftheecologicalzones.Bypopulationstructureanalysis,the

99varietiesweredividedintothreesubgroups.Clusteringresultswereincoincidentwiththeresultsof

populationstructure.The31accessionshaveahighdegreeofmixedancestryandcomplexgeneticbackground.

犓犲狔狑狅狉犱狊:sweetpotato;registeredvarieties;SSRmarkers;geneticdiversity

  MN

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Table1 Informationofmaterialstestedinthisstudy

¹º

Code»t

NameüBy

Origin¹º

Code»t

NameüBy

Origin

01 ¼N24ºLongshuNo.24 ½=Fujian 10 ¾¿1ºJifenNo.1 d" Hebei

02 ½N24ºFushuNo.24 ½=Fujian 11 ¾N982Jishu982 d" Hebei

03 ½N604Fushu604 ½=Fujian 12 À×Á Weiduoli d" Hebei

04 ÂN819Rongshu819 ½=Fujian 13 ¾N4ºJishuNo.4 d" Hebei

05 KÃN6º XuzishuNo.6 vwJiangsu 14 ¾N332Jishu332 d" Hebei

06 KN31Xushu31 vwJiangsu 15 ¾N65Jishu65 d" Hebei

07 KN33Xushu33 vwJiangsu 16 ¾N71Jishu71 d" Hebei

08 KÄN34Xuyushu34 vwJiangsu 17 ¾N98Jishu98 d" Hebei

09 KÄN35Xuyushu35 vwJiangsu 18 ¾N99Jishu99 d" Hebei

31217a            OPQ

,È

:r'MN4567SSR�5\]×"Þ8¨

Ål1 ContinuedTable1

¹º

Code»t

NameüBy

Origin¹º

Code»t

NameüBy

Origin

19 ¾ÃN2ºJizishuNo.2 d" Hebei 60 3N14Jinshu14 ½=Fujian

20 ÆN10ºLuoshuNo.10 d| Henan 61 ½gN18ºFucaishuNo.18 ½=Fujian

21 ÆN11Luoshu11 d| Henan 62 ÇN21Jishu21 ÈYShandong

22 ÆÃN1ºLuozishuNo.1 d| Henan 63 ÉgN2ºEcaishuNo.2 Ê" Hubei

23 ËN12Zhanshu12 -Y Guangdong 64 ÉN6ºEshuNo.6 Ê" Hubei

24 ËN271Zhanshu271 -Y Guangdong 65 ÉN7ºEshuNo.7 Ê" Hubei

25 ËN407Zhanshu407 -Y Guangdong 66 ÉN8ºEshuNo.8 Ê" Hubei

26 ËÃN2ºZhanzishuNo.2 -Y Guangdong 67 ÉN10ºEshuNo.10 Ê" Hubei

27 ÌN5º WanshuNo.5 )ÍChongqing 68 ÉN11Eshu11 Ê" Hubei

28 ÌN6º WanshuNo.6 )ÍChongqing 69 ÉÃN13Ezishu13 Ê" Hubei

29 ÌN7º WanshuNo.7 )ÍChongqing 70 wN16ºSushuNo.16 vwJiangsu

30 ÌN8º WanshuNo.8 )ÍChongqing 71 ÎN17Quanshu17 ½=Fujian

31 ÌN9º WanshuNo.9 )ÍChongqing 72 ÏÃN1º NingzishuNo.1 vwJiangsu

32 ÌN10º WanshuNo.10 )ÍChongqing 73 ¼N9ºLongshuNo.9 ½=Fujian

33 ÌÃN56Wanzishu56 )ÍChongqing 74 ÇN18Jishu18 ÈYShandong

34 KÃN8º XuzishuNo.8 vwJiansu 75 ÇK23Jixu23 ÈYShandong

35 ÐN12Shangshu12 d| Henan 76 ÇN25Jishu25 ÈYShandong

36 ÑN5Liaoshu5 ÑÏLiaoning 77 ÇN26Jishu26 ÈYShandong

37 ÑN15Liaoshu15 ÑÏLiaoning 78 ÇN27Jishu27 ÈYShandong

38 ¼Ã4ºLongziNo.4 ½=Fujian 79 ÇÃN3ºJizishuNo.3 ÈYShandong

39 ¼N31ºLongshuNo.31 ½=Fujian 80 ÒÏ16º Qining16 ÈYShandong

40 ÓÔÕ³NSanxiahongxinshu Ê" Hubei 81 ÒÏ18º QiningNo.18 ÈYShandong

41 ½N76Fushu76 vwJiangsu 82 Öw4ºPengsuNo.4 )ÍChongqing

42 ×MNçºShiganshuNo.1 ØÙSichuan 83 ÚN2ºLinshuNo.2 ÈYShandong

43 3N3ºJinshuNo.3 -Y Guangdong 84 ÒÏ12º QiningNo.12 ÈYShandong

44 3È208Jinshan208 ØÙSichuan 85 ÒÏ13º QiningNo.13 ÈYShandong

45 3È57Jinshan208 d| Henan 86 KN22Xushu22 vwJiangsu

46 NÛ1ºShulvNo.1 ØÙSichuan 87 ÜMN3ºJinganshuNo.3 È!Shanxi

47 ÝN25Yanshu25 ØÙSichuan 88 ÞN14Taishu14 ÈYShandong

48 ßN5º YanshuNo.5 ØÙSichuan 89 KN18Xushu18 ÈYShandong

49 ÏÃN4º NingzishuNo.4 d| Henan 90 ÉN9ºEshuNo.9 Ê" Hubei

50 àN524Suishu524 d| Henan 91 ÐN8ºShangshu8 d| Henan

51 -N87Guangshu87 ½=Fujian 92 KnN2º XuxinshuNo.2 vwJiangsu

52 áÄÃ11Mianyuzi11 ½=Fujian 93 KN32Xushu32 vwJiangsu

53 âÕ23ºZhenghongNo.23 ½=Fujian 94 KN24Xushu24 vwJiangsu

54 áÃN9º MianzishuNo.9 ÈYShandong 95 wN22ºSushuNo.22 vwJiangsu

55 áÄÃ12Mianyuzi12 Ê" Hubei 96 wN25Sushu25 vwJiangsu

56 á|N10º MiannanshuNo.10 Ê" Hubei 97 ½N9ºFushuNo.9 ½=Fujian

57 âÕ22Zhenghong22 d| Henan 98 ÐN9ºShangshuNo.9 d| Henan

58 âN20Zhengshu20 d| Henan 99 KN34Xushu34 vwJiangsu

59 ÎN19Quanshu19 ½=Fujian

4121 ! " # $ % &                   39ã

Ç�CTAB£¤¥MNqäå DNA �Éz

。�

Nanodrop��DNAæ¿Åç¿

,"6DNA«è

;

,éê[HCæ¿50ng/μL��Gë

。PCRÑ

Ò �3ÔÏ$

,>1ÔÏ$[SSRìZÏ$o

M13(TGTAAAACGACGGCCAGT)¢r½í�Ï

$

,îTPM13Ï$

,>2ÔÏ$[ïð���5�

_�Ï$

,>3ÔÏ$[ñò�óZÏ$

。PCRó

+å÷ô[20μL,IJ1×3õ Mix17.4μL,50

ng/μLDNA"61μL,10μmol/LTPM13Ï$

0.15μL,10μmol/L_�Ï$0.65μL,10μmol/L

óZÏ$0.80μL。PCRó+�p[98℃ö÷Þ

2min,98℃÷Þ10s,55~57℃øù30s,72℃ú

û10s,35äüý

;72℃þ8úû1min,4℃Cb

ÑÒB$_������ÿ!¤¥8�

,��[

ABI3730XLDNAanalyzer(AppliedBiosystem)。

28®Ï$pq);MN2pqÅ"#q:;$,

>-

,ü/r'Fs}%

[20]ÅÂw%&

,N"'(

1X$1�ê)*+«´

(l2)。

1.3 YZ[\R>?

��GeneMarker2.2��ãÝ,�-.:;

¤¥8¨

,);89C���/0ÝÓ³"6ÑÒ

12�}4

,3�4�5T˶67

[21]。%4�

[0,ê4�[1,O´

“01”:;<

。g�DPS��

-�Nei\]��

,0;ãúg� MEGAXr

, 

�NeighborJoining£°=¹f,^á

。�Struc

ture2.3.4:;â8��®99���¤¥8¨

,Ë

:>0˶67

[3]。9¥¯:;ãResult6�F

];StructureHarvesterS<�é

, Á犓 =

[22]。

2 ¯Ío8¨

2.1 犛犛犚]^;_=>?

 �28®×ØÞÏ$®99���2pq

DNA¤¥ÑÒ

,ÐÑÒÓ162ÔÕÖ

,úr×ØÞ

ÕÖ156Ô

,×ØÙ[96.30%,IÛÚ®Ï$ÜÝ

5.57Ô×ØÞÕÖ

(l2)。úr

,Ï$P289、P292

ÜÝ�×ØÞÕÖ>×

(12Ô

);SPGS3、P273ÜÝ

�×ØÞÕÖ>°

(2Ô

);Ú®Ï$ÑÒ×ØÞÕ

Ö:@?�2~12Ô��

,@A

,Ï$SPES3S�

�"6ÑÒÓ5ÔÕÖ

(425bp、430bp、436bp、443

bpÅ449bp,B1,A),S�ä"6

“ËÃN2º

ӄ

ÒÓ3ÔÕÖ

(430bp、436bpÅ443bp,B1,B)。

2.2 2367`9:;<=>?

2.2.1 9:ab>? ��FC)ÿÞóR�28

®Ï$�SSR:;

,-���XØNz67��\

]��

,¤çD8¨��yz67��\]��

(l3)。

A.Ï$SPES3S��MN"6��ÑÒ12}4

;B.Ï$SPES3SMN"6

‘ËÃN2º

’�ÑÒ12}4

B1 Ï$SPES3Sõ8MN"6��ÑÒ¯Í

A.AmplifiedfragmentsofmultiplesamplesbyprimerSPES3;B.Amplifiedfragmentsof‘ZhanzishuNo.2’byprimerSPES3

Fig.1 AmplificationresultsofdifferentsweetpotatovarietiesbyprimerSPES3

51217a            OPQ

,È

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U2 Vcdef28g犛犛犚]^hi;_=>?

Table2 Informationandpolymorphismofthe28SSRprimerpairsusedinthisstudy

Ï$¹º

Primercode

LÏ$pq

Forwardprimersequence;Ï$pq

Reverseprimersequence

øù

¯¿

Tm

ÑÒ12

}4

Size/bp

ÑÒ/

*:

Bands

×ØÞ

/*:

Polymorphicbands

SPGS1 CTCGCTCACGATTGATGATG CGGAGTGGTAGGGCTAAACC 59.0 290~313 6 5

SPGS2 AGACTGGACTCCCAGAAGCA CAAGCAGTCAGAAGTCGACAA 56.8 274~351 5 5

SPGS3 CCGATCATTCCCAAACTCAT AGCAGGGGAGACGTAAGGAT 57.8 244~253 3 2

SPGS4 ATCAGAGCCTGGCAAAGAAA GGGGAACTTCAGCTAAGCAA 57.5 225~241 5 5

SPES1 AATGCCAACCAAAGCCATAG CGATGACAAAGCAGCTGAAG 57.9 301~326 6 6

SPES2 TCGGAACGGAGATAGATTGG AAGCAAGAAAAAGAAGTGAAGGAA 59.0 241~275 7 7

SPES3 ATGACATCCCAAGGAGCATC GAGGTTGAGGGCGTATCTGA 57.4 425~449 5 5

C32 GACCTGCGAATCGAAATCTT CTTGGACTTCCTCTGCCTTG 55.8 240~263 9 9

C48 ATCCACTTCCGTTCATCCAC CGCAAATCCCAGGTCTTTTA 55.8 197~232 10 10

C51 GAGGACTTCTCCGACCAGTG ACCAGATCCGTGTTCGTCTC 61.9 317~334 8 8

C56 CCACCCTAAGACTGAAGAAGAAA GCAAAGCAAACAAAGCAAAA 51.6 181~187 4 4

Z37 GGCGACTGTAATGTGGTGAA CGGGAGGTATCTTGGATTGA 57.8 261~282 8 8

P238 GTGGGCGGGCAATCAAATG TGCATGACCACCTATGTCCC 59.7 256~274 6 6

P243 CTGTCCTCTACACAGGCCG GGGTAGATATTGCTGCCAAGAC 61.0 211~220 3 3

P245 GCTCGATTGCCCAAAGTCC AGCCAACTTGCACTAGTCTTC 58.8 178~291 4 4

P268 GCCCGAGGATTCTAGTGGG GAAGGGTCAGGAACCGAGG 61.9 300~468 6 6

P273 CGCGGTCCCATTTAAGCAC GCCAAACAATCCCATTGCC 58.6 269~275 3 2

P279 GGCAAGCTGAAGATCGCTG TGCTACAAGCACCCAATGC 58.6 433~441 4 4

P281 GCGGCAAGGCGATTTGG GGATGCAGTTGAAGGGTGC 59.6 294~312 4 3

P283 GATCCGTTATCGGCCGGAG CGTGAATCGCCTGAGTTCC 60.8 171~177 3 3

P289 AGGTCCAAGAGCTTCACGG ACCAGACAACGAGCTGAGG 59.7 235~295 12 12

P292 GGCAACCATTCCACTGCTC GATGATCGCTTCACTGCCG 57.9 184~216 12 12

P303 CTGTTCGTCTGCCATTTCCC CAGCATTCCAGATGCTCGG 59.8 174~189 5 4

P306 AGAGGTGAAGGGTGAGCATC AATTGACACCAAGAGTAGGAAAC 58.2 289~309 6 6

P309 TCTAAGAATGGCCCGGGAG CTCTTCTTCGGTGGTTGGAC 59.7 249~261 5 4

P324 TGCGAACGCGGATGTAAAG CTCGACGCTTGGGAAATGC 58.7 225~234 4 4

P345 TGTGTTCGTTGCGGACTTC TGTGATGCCTTAGACTTGCG 57.7 214~225 5 5

P248 AATCGCAAGGGCAAGGATG AGAGCGTGGAAGGGTTACG 58.6 384~400 4 4

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ZHUXZ,TIANXW,WANGSB,犲狋犪犾.Developmentpros

pectandeconomybenefitofsweetpotato[J].犑狅狌狉狀犪犾狅犳

犛犺犪狀狓犻犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲狊,2011,39(4):386388.

[2] WADLPA,OLUKOLUBA,BRANHAMSE,犲狋犪犾.Ge

neticdiversityandpopulationstructureoftheUSDAsweet

potato(犐狆狅犿狅犲犪犫犪狋犪狋犪狊)germplasmcollectionsusingGB

Spoly[J].犉狉狅狀狋犻犲狉狊犻狀犘犾犪狀狋犛犮犻犲狀犮犲,2018,9:1166.

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,x {

,|}~

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"Þoå÷¯°8¨

[J].#$\]AB%&

,2018,19(2):

243251.

SUYJ,WANGJ,DAIXB,犲狋犪犾.Geneticdiversityandpop

ulationstructureanalysisof303sweetpotatolandracesusing

SSRmarkers[J].犑狅狌狉狀犪犾狅犳犘犾犪狀狋犌犲狀犲狋犻犮犚犲狊狅狌狉犮犲狊,2018,

19(2):243251.

[4] ZHANGDP,ROSSELG,KRIEGNERA,犲狋犪犾.AFLPas

sessmentofdiversityinsweetpotatofromLatinAmericaand

thePacificregion:Itsimplicationsonthedispersalofthecrop

[J].犌犲狀犲狋犻犮犚犲狊狅狌狉犮犲狊犪狀犱犆狉狅狆犈狏狅犾狌狋犻狅狀,2004,51(2):

115120.

[5] �­�

,¥��

,� �

,È.Ê|MN67AFLP�5�\

]��8¨

[J].vwFs%&

,2010,26(4):706710.

ZHANGCF,HUANGYL,ZHOUH,犲狋犪犾.Geneticdiffer

enceofsweetpotatovarietiesinHunanProvinceanalyzedby

AFLPmarkers[J].犑犻犪狀犵狊狌犑狅狌狉狀犪犾狅犳 犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻

犲狀犮犲狊,2010,26(4):706710.

[6] JARRETRL,AUSTINDF.Geneticdiversityandsystematic

relationshipsinsweetpotato [犐狆狅犿狅犲犪犫犪狋犪狋犪狊 (L.)Lam.]

andrelatedspeciesasrevealedbyRAPDanalysis[J].犌犲狀犲狋犻犮

犚犲狊狅狌狉犮犲犪狀犱犆狉狅狆犈狏狅犾狌狋犻狅狀,1994,41(3):16517.

[7] xÕ�

,� Õ

,x8�

,È.30är'MNiU67�

RAPDX�BÕ°=µ\]÷�8¨

[J].89#$^7

2009,7(5):879884.

WANGH Y,ZHAIH,WANGYP,犲狋犪犾.RAPDfinger

printsandgeneticvariationsofthe30mainsweetpotatovarie

tiesinChina[J].犕狅犾犲犮狌犾犪狉犘犾犪狀狋犅狉犲犲犱犻狀犵,2009,7(5):

879884.

[8] ���

,���

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,È.2�SSR�5�132�MN7

HX�BÕ�°=µ\]×"Þ8¨

[J].#$\]AB%

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,2018,19(5):904911.

NIELY,LIAX,QINZ,犲狋犪犾.ConstructionofDNAfinger

printsandanalysisofgeneticdiversitybasedonSSRmarkers

for132sweetpotatogermplasms[J].犑狅狌狉狀犪犾狅犳犘犾犪狀狋犌犲狀犲狋犻犮

犚犲狊狅狌狉犮犲狊,2018,19(5):904911.

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[9] �n�.2�SSR、SNPÅOØ%�5�MN7HAB\]×

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,2016.

[10] SMITHJSC,CHINECL,SHUH,犲狋犪犾.Anevaluation

oftheutilityofSSRlociasmolecularmarkersinmaize(犣犲犪

犿犪狔狊L.):ComparisonswithdatafromRFLPSandpedigree

[J].犜犺犲狅狉犲狋犻犮犪犾犪狀犱犃狆狆犾犻犲犱犌犲狀犲狋犻犮狊,1997,95(12):163

173.

[11] NAIRAGH,VIDYAP,AMBUV,犲狋犪犾.Geneticdiversity

studiesincultivatedsweetpotato (犐狆狅犿狅犲犪犫犪狋犪狋犪狊 (L.)

Lam)revealedbysimplesequencerepeatmarkers[J].犐狀狋犲狉

狀犪狋犻狅狀犪犾犑狅狌狉狀犪犾狅犳 犃犱狏犪狀犮犲犱 犅犻狅狋犲犮犺狀狅犾狅犵狔 犚犲狊犲犪狉犮犺,

2017,7:3348.

[12] ZAWEDDEBM,GHISAINM,MAGEMBEE,犲狋犪犾.Char

acterizationofthegeneticdiversityofUgandassweetpotato

(犐狆狅犿狅犲犪犫犪狋犪狋犪狊)germplasmusingmicrosatellitesmarkers

[J].犌犲狀犲狋犻犮犚犲狊狅狌狉犮犲狊犪狀犱犆狉狅狆犈狏狅犾狌狋犻狅狀,2015,62(4):

501513.

[13] wçd

,x {

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,È.MNϤ7SSR\]×"Þ

[J].vwFs%&

,2018,34(5):984997.

SUYJ,WANGJ,HUOKS,犲狋犪犾.Geneticdiversityanaly

sisofintroducedsweetpotatogermplasm collections[J].

犑犻犪狀犵狊狌犑狅狌狉狀犪犾狅犳犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻犲狀犮犲狊,2018,34(5):

984997.

[14] wçd

,���

,x {

,È.g�ÅhiMN7HAB\

]×"Þ8¨

[J].#$\]AB%&

,2018,19(1):5764.

SUYJ,DOGLX,WANGJ,犲狋犪犾.Geneticdiversityinveg

etableandornamentalsweetpotatogermplasm[J].犑狅狌狉狀犪犾

狅犳犘犾犪狀狋犌犲狀犲狋犻犮犚犲狊狅狌狉犮犲狊,2018,19(1):5764.

[15] SCHUELKEM.Aneconomicmethodforthefluorescentla

belingofPCRfragments[J].犖犪狋狌狉犲犅犻狅狋犲犮犺狀狅犾狅犵狔,2000,

18:233234.

[16] ö B

,x �

,uPm

,È.TPM13SSR��µúS�Í

7HAB\]×"Þ+Wr�+�

[J].#$\]AB%&

2011,12(2):228233.

GAOY,WANGK,TIANLM,犲狋犪犾.TPM13SSRtech

niqueanditsapplicationsinanalysisofgeneticdiversityfor

applegermplasmresources[J].犑狅狌狉狀犪犾狅犳犘犾犪狀狋犌犲狀犲狋犻犮犚犲

狊狅狌狉犮犲狊,2011,12(2):228233.

[17] ZHUYF,QINGC,HUJ,犲狋犪犾.Fingerprintingandvarie

tyidentificationofrice(犗狉狔狕犪狊犪狋犻狏犪L.)basedonsimplese

quencerepeatmarkers[J].犘犾犪狀狋犗犿犻犮狊犑狅狌狉狀犪犾,2012,5

(4):421426.

[18] rß�.G$6789�5ÀÁµX�BÕ°=+W

[D].

�L

:�v}%

,2013.

[19] ·q¸

,��I

,��¤

,È.�C£� ÉzMN¬DNA

Ô��Sà

[J].¡¢Fs1%

,2009,37(31):15157

15159.

WULH,XIEDP,ZHANGYC,犲狋犪犾.Optimizationonthe

conditionsforrapidextractionofDNAfromsweetpotatoleav

esbymicromethod[J].犑狅狌狉狀犪犾狅犳犃狀犺狌犻犃犵狉犻犮狌犾狋狌狉犪犾犛犮犻

犲狀犮犲狊,2009,37(31):1515715159.

[20] MENGYS,ZHAON,LIH,犲狋犪犾.SSRfingerprintingof

203sweetpotato(犐狆狅犿狅犲犪犫犪狋犪狋犪狊(L.)Lam.)varieties[J].

犑狅狌狉狀犪犾狅犳犐狀狋犲犵狉犪狋犻狏犲犃犵狉犻犮狌犾狋狌狉犲,2018,17(1):8693.

[21] x£è

,¤Õ¥

,O¦0

,È.���ÿ!����r��

ñÿ!§­µ-p8¨

[J].89#$^7

,2007,5(6):

890894.

WANGFG,YIHM,ZHAOJR,犲狋犪犾.Analysisoftheab

normalpeaktypesincapillaryelectrophoresiswithfluores

centlabeled[J].犕狅犾犲犮狌犾犪狉犘犾犪狀狋犅狉犲犲犱犻狀犵,2007,5(6):

890894.

[22] EVANNO G,REGNAUTS,GOUDETJ.Detectingthe

numberofclustersofindividualsusingthesoftwareSTRUC

TURE:asimulationstudy[J].犕狅犾犲犮狌犾犪狉犈犮狅犾狅犵狔,2005,14

(8):26112620.

[23] ��4

,� Ï

,� §

,È.¨97H\]×"Þµå÷

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[J].#$%&

,2019,54(1):5863.

XIELF,LIN,LIY,犲狋犪犾.SRAPanalysisofgeneticdiver

sityandpopulationstructureofeggplantgermplasm[J].犆犺犻

狀犲狊犲犅狌犾犾犲狋犻狀狅犳犅狅狋犪狀狔,2019,54(1):5863.

[24] Oª©

,�

,« ¬

,È.MN7HAB\]­ÁÞµ

\]×"ÞSSR8¨

[J].#$\]AB%&

,2011,12(3):

389395.

ZHAODL,ZHENGLT,TANGJ,犲狋犪犾.Geneticstability

anddiversityofsweetpotatogermplasmwithSSR[J].犑狅狌狉

狀犪犾狅犳犘犾犪狀狋犌犲狀犲狋犻犮犚犲狊狅狌狉犮犲狊,2011,12(3):389395.

[25] wçd.g�ÅhiMN7HAB×"Þo6H8¨

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