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,2019,39(7):1212-1220犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
犱狅犻:10.7606/j.issn.10004025.2019.07.1212 http://xbzwxb.alljournal.net
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犌犲狀犲狋犻犮犇犻狏犲狉狊犻狋狔狅犳狋犺犲犚犲犵犻狊狋犲狉犲犱犛狑犲犲狋狆狅狋犪狋狅犞犪狉犻犲狋犻犲狊
犻狀犆犺犻狀犪犫狔犛犛犚犕犪狉犽犲狉狊
ZHAOLukuan1,SUYijun1,DAIXibin1,WANGYao2,YUANRui1,CAOQinghe
1
(1SweetpotatoResearchInstitute,ChinaAgriculturalAcademyofSciences,JiangsuXuhuaiRegionalAgriculturalAcademyof
Sciences,Xuzhou,Jiangsu221131,China;2CollegeofHorticulture,UniversityofHainan,Haikou570228,China)
犃犫狊狋狉犪犮狋:BasedonTPM13SSRfluorescentcapillarydetectiontechnology,weanalyzedthegeneticdiver
sityof99registeredsweetpotatovarieties.The“01”databaseoftheregisteredvarietieswasestablishedby
SSRmarkers.TheNeigeneticdistanceamongdifferentvarietieswascalculatedusingtheDPSsoftware.
TheNeighborJoiningmethodinMEGAwasusedforclusteranalysis,andthegeneticstructureoftheac
cessionswasanalyzedbytheadmixtureancestrymodelinStructuresoftware.Thegeneticcompositionof
thevarietiesandthegeneticrelationshipamongthevarietieswerediscussed,whichrevealedthesimilarity
ofthesweetpotatovarietiesattheDNAlevelandprovidedreferencesforvarietyevaluation,excellentpar
entselectionandvarietyimprovement.Theresultsshowedthat:(1)atotalof162reproduciblebands
wereamplifiedfrom28primerpairswithanaverageof5.57bandsandpolymorphicratewas96.30%.(2)
Thedendrogramof99registeredvarietieswasconstructed.Andthegeneticdistanceof99varietiesranged
from0to0.4646withanaverageof0.3077.Clusteranalysisat0.2670geneticdistancecandivide99va
rietiesintothreegroups.Sweetpotatovarietiesfromthesameagroecologicalzonesorcloserelationship
wereclusteredinthesamegroups.Thevarietiesfromdifferentagroecologicalzoneswererelativelyscat
teredindifferentgroups.(3)Thegeneticbackgroundamongaccessionsisrelativelynarrow.Andsimilari
tyofsomevarietiesishigherinsomecertainoftheecologicalzones.Bypopulationstructureanalysis,the
99varietiesweredividedintothreesubgroups.Clusteringresultswereincoincidentwiththeresultsof
populationstructure.The31accessionshaveahighdegreeofmixedancestryandcomplexgeneticbackground.
犓犲狔狑狅狉犱狊:sweetpotato;registeredvarieties;SSRmarkers;geneticdiversity
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Table1 Informationofmaterialstestedinthisstudy
¹º
Code»t
NameüBy
Origin¹º
Code»t
NameüBy
Origin
01 ¼N24ºLongshuNo.24 ½=Fujian 10 ¾¿1ºJifenNo.1 d" Hebei
02 ½N24ºFushuNo.24 ½=Fujian 11 ¾N982Jishu982 d" Hebei
03 ½N604Fushu604 ½=Fujian 12 À×Á Weiduoli d" Hebei
04 ÂN819Rongshu819 ½=Fujian 13 ¾N4ºJishuNo.4 d" Hebei
05 KÃN6º XuzishuNo.6 vwJiangsu 14 ¾N332Jishu332 d" Hebei
06 KN31Xushu31 vwJiangsu 15 ¾N65Jishu65 d" Hebei
07 KN33Xushu33 vwJiangsu 16 ¾N71Jishu71 d" Hebei
08 KÄN34Xuyushu34 vwJiangsu 17 ¾N98Jishu98 d" Hebei
09 KÄN35Xuyushu35 vwJiangsu 18 ¾N99Jishu99 d" Hebei
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Ål1 ContinuedTable1
¹º
Code»t
NameüBy
Origin¹º
Code»t
NameüBy
Origin
19 ¾ÃN2ºJizishuNo.2 d" Hebei 60 3N14Jinshu14 ½=Fujian
20 ÆN10ºLuoshuNo.10 d| Henan 61 ½gN18ºFucaishuNo.18 ½=Fujian
21 ÆN11Luoshu11 d| Henan 62 ÇN21Jishu21 ÈYShandong
22 ÆÃN1ºLuozishuNo.1 d| Henan 63 ÉgN2ºEcaishuNo.2 Ê" Hubei
23 ËN12Zhanshu12 -Y Guangdong 64 ÉN6ºEshuNo.6 Ê" Hubei
24 ËN271Zhanshu271 -Y Guangdong 65 ÉN7ºEshuNo.7 Ê" Hubei
25 ËN407Zhanshu407 -Y Guangdong 66 ÉN8ºEshuNo.8 Ê" Hubei
26 ËÃN2ºZhanzishuNo.2 -Y Guangdong 67 ÉN10ºEshuNo.10 Ê" Hubei
27 ÌN5º WanshuNo.5 )ÍChongqing 68 ÉN11Eshu11 Ê" Hubei
28 ÌN6º WanshuNo.6 )ÍChongqing 69 ÉÃN13Ezishu13 Ê" Hubei
29 ÌN7º WanshuNo.7 )ÍChongqing 70 wN16ºSushuNo.16 vwJiangsu
30 ÌN8º WanshuNo.8 )ÍChongqing 71 ÎN17Quanshu17 ½=Fujian
31 ÌN9º WanshuNo.9 )ÍChongqing 72 ÏÃN1º NingzishuNo.1 vwJiangsu
32 ÌN10º WanshuNo.10 )ÍChongqing 73 ¼N9ºLongshuNo.9 ½=Fujian
33 ÌÃN56Wanzishu56 )ÍChongqing 74 ÇN18Jishu18 ÈYShandong
34 KÃN8º XuzishuNo.8 vwJiansu 75 ÇK23Jixu23 ÈYShandong
35 ÐN12Shangshu12 d| Henan 76 ÇN25Jishu25 ÈYShandong
36 ÑN5Liaoshu5 ÑÏLiaoning 77 ÇN26Jishu26 ÈYShandong
37 ÑN15Liaoshu15 ÑÏLiaoning 78 ÇN27Jishu27 ÈYShandong
38 ¼Ã4ºLongziNo.4 ½=Fujian 79 ÇÃN3ºJizishuNo.3 ÈYShandong
39 ¼N31ºLongshuNo.31 ½=Fujian 80 ÒÏ16º Qining16 ÈYShandong
40 ÓÔÕ³NSanxiahongxinshu Ê" Hubei 81 ÒÏ18º QiningNo.18 ÈYShandong
41 ½N76Fushu76 vwJiangsu 82 Öw4ºPengsuNo.4 )ÍChongqing
42 ×MNçºShiganshuNo.1 ØÙSichuan 83 ÚN2ºLinshuNo.2 ÈYShandong
43 3N3ºJinshuNo.3 -Y Guangdong 84 ÒÏ12º QiningNo.12 ÈYShandong
44 3È208Jinshan208 ØÙSichuan 85 ÒÏ13º QiningNo.13 ÈYShandong
45 3È57Jinshan208 d| Henan 86 KN22Xushu22 vwJiangsu
46 NÛ1ºShulvNo.1 ØÙSichuan 87 ÜMN3ºJinganshuNo.3 È!Shanxi
47 ÝN25Yanshu25 ØÙSichuan 88 ÞN14Taishu14 ÈYShandong
48 ßN5º YanshuNo.5 ØÙSichuan 89 KN18Xushu18 ÈYShandong
49 ÏÃN4º NingzishuNo.4 d| Henan 90 ÉN9ºEshuNo.9 Ê" Hubei
50 àN524Suishu524 d| Henan 91 ÐN8ºShangshu8 d| Henan
51 -N87Guangshu87 ½=Fujian 92 KnN2º XuxinshuNo.2 vwJiangsu
52 áÄÃ11Mianyuzi11 ½=Fujian 93 KN32Xushu32 vwJiangsu
53 âÕ23ºZhenghongNo.23 ½=Fujian 94 KN24Xushu24 vwJiangsu
54 áÃN9º MianzishuNo.9 ÈYShandong 95 wN22ºSushuNo.22 vwJiangsu
55 áÄÃ12Mianyuzi12 Ê" Hubei 96 wN25Sushu25 vwJiangsu
56 á|N10º MiannanshuNo.10 Ê" Hubei 97 ½N9ºFushuNo.9 ½=Fujian
57 âÕ22Zhenghong22 d| Henan 98 ÐN9ºShangshuNo.9 d| Henan
58 âN20Zhengshu20 d| Henan 99 KN34Xushu34 vwJiangsu
59 ÎN19Quanshu19 ½=Fujian
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A.AmplifiedfragmentsofmultiplesamplesbyprimerSPES3;B.Amplifiedfragmentsof‘ZhanzishuNo.2’byprimerSPES3
Fig.1 AmplificationresultsofdifferentsweetpotatovarietiesbyprimerSPES3
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Table2 Informationandpolymorphismofthe28SSRprimerpairsusedinthisstudy
Ï$¹º
Primercode
LÏ$pq
Forwardprimersequence;Ï$pq
Reverseprimersequence
øù
¯¿
Tm
ÑÒ12
}4
Size/bp
ÑÒ/
*:
Bands
×ØÞ
/*:
Polymorphicbands
SPGS1 CTCGCTCACGATTGATGATG CGGAGTGGTAGGGCTAAACC 59.0 290~313 6 5
SPGS2 AGACTGGACTCCCAGAAGCA CAAGCAGTCAGAAGTCGACAA 56.8 274~351 5 5
SPGS3 CCGATCATTCCCAAACTCAT AGCAGGGGAGACGTAAGGAT 57.8 244~253 3 2
SPGS4 ATCAGAGCCTGGCAAAGAAA GGGGAACTTCAGCTAAGCAA 57.5 225~241 5 5
SPES1 AATGCCAACCAAAGCCATAG CGATGACAAAGCAGCTGAAG 57.9 301~326 6 6
SPES2 TCGGAACGGAGATAGATTGG AAGCAAGAAAAAGAAGTGAAGGAA 59.0 241~275 7 7
SPES3 ATGACATCCCAAGGAGCATC GAGGTTGAGGGCGTATCTGA 57.4 425~449 5 5
C32 GACCTGCGAATCGAAATCTT CTTGGACTTCCTCTGCCTTG 55.8 240~263 9 9
C48 ATCCACTTCCGTTCATCCAC CGCAAATCCCAGGTCTTTTA 55.8 197~232 10 10
C51 GAGGACTTCTCCGACCAGTG ACCAGATCCGTGTTCGTCTC 61.9 317~334 8 8
C56 CCACCCTAAGACTGAAGAAGAAA GCAAAGCAAACAAAGCAAAA 51.6 181~187 4 4
Z37 GGCGACTGTAATGTGGTGAA CGGGAGGTATCTTGGATTGA 57.8 261~282 8 8
P238 GTGGGCGGGCAATCAAATG TGCATGACCACCTATGTCCC 59.7 256~274 6 6
P243 CTGTCCTCTACACAGGCCG GGGTAGATATTGCTGCCAAGAC 61.0 211~220 3 3
P245 GCTCGATTGCCCAAAGTCC AGCCAACTTGCACTAGTCTTC 58.8 178~291 4 4
P268 GCCCGAGGATTCTAGTGGG GAAGGGTCAGGAACCGAGG 61.9 300~468 6 6
P273 CGCGGTCCCATTTAAGCAC GCCAAACAATCCCATTGCC 58.6 269~275 3 2
P279 GGCAAGCTGAAGATCGCTG TGCTACAAGCACCCAATGC 58.6 433~441 4 4
P281 GCGGCAAGGCGATTTGG GGATGCAGTTGAAGGGTGC 59.6 294~312 4 3
P283 GATCCGTTATCGGCCGGAG CGTGAATCGCCTGAGTTCC 60.8 171~177 3 3
P289 AGGTCCAAGAGCTTCACGG ACCAGACAACGAGCTGAGG 59.7 235~295 12 12
P292 GGCAACCATTCCACTGCTC GATGATCGCTTCACTGCCG 57.9 184~216 12 12
P303 CTGTTCGTCTGCCATTTCCC CAGCATTCCAGATGCTCGG 59.8 174~189 5 4
P306 AGAGGTGAAGGGTGAGCATC AATTGACACCAAGAGTAGGAAAC 58.2 289~309 6 6
P309 TCTAAGAATGGCCCGGGAG CTCTTCTTCGGTGGTTGGAC 59.7 249~261 5 4
P324 TGCGAACGCGGATGTAAAG CTCGACGCTTGGGAAATGC 58.7 225~234 4 4
P345 TGTGTTCGTTGCGGACTTC TGTGATGCCTTAGACTTGCG 57.7 214~225 5 5
P248 AATCGCAAGGGCAAGGATG AGAGCGTGGAAGGGTTACG 58.6 384~400 4 4
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Table3 Geneticdistanceofsweetpotatovarietiesindifferentagroecologicalzones
XØNz
Ecologicalzone
|Y¨©Nz
Southernsummerautumnsweetpotatozone
¥x¦§¤¨Nz
SpringsummersweetpotatozoneinHuangHuaiRiverBasin
|Y©ªNz
Southernautumnwintersweetpotato
zone
`v¦§¨Nz
SummersweetpotatozoneintheYangtze
RiverBasin
"Y¤Nz
Northernspringsweetpotatozone
|Y¨©NzSouthernsummerautumnsweetpotatozone
0
¥x¦§¤¨NzSpringsummersweetpotatozoneinHuangHuaiRiverBasin
0.316 0
|Y©ªNzSouthernautumnwintersweetpotatozone
0.313 0.316 0
`v¦§¨NzSummersweetpotatozoneintheYangtzeRiverBasin
0.314 0.301 0.305 0
"Y¤NzNorthernspringsweetpotatozone 0.355 0.341 0.349 0.323 0
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Fig.2 Neighborjoiningtreefor99registeredsweetpotatovarietiesbasedonSSRmarkers
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