1

TMHMA

:

2015

2

1. . . . 2. . . . 3. . . Soxhlet . . BHT PG 4. (GC-FID) . . . 5. , HPLC. 6. (AAS). 7. GC/MS. 8. R.

3

.

1. .

2. :

. .

. .

3. ( 0-10 ) , .

4. (0). . . (5). .

5. , .

6. , ,

4

. . . .

7. .

8. .

.

5

1.

.

33,33 gr 17ml , 10-11 cm . 30 min 15-16 C . . .

. % . (100-105 C) . % .

.

10% 20%. .

6

2.

.

1gr .

Kottstorfer ml /10 100gr .

Burstyn ml /10 100ml .

% ( Kottstorfer 0.2823).

.

:

8-10gr . 3-4 ( 1%) /10 .

7

.

(A) ottstorfer 1 gr .

,

():

A . A . ..:

- : ~ 190

- : ~ 225-230

- & : ~ 250

1.

28gr , 1 . , 10gr , .

8

2. /2 Cl

1 . .

1-2gr 250ml 25ml . 30min . . , 15min . (1%) /2 Cl.

, , , /2 Cl.

:

= [(-) 28] /

ml /2 HCl , ml /2 HCl gr.

9

3.

, . . . :

1. , , .

2. ( ). , .

3. .

. Soxhlet

Soxhlet.

1. , .

2. 5 gr ( 0.1gr), ( 110 C) .

3. (0.01 gr) .

4. 11/2 .

10

. 2-3 /sec.

5. 21/2 . , , . 0 C.

6. (105 C) , .

% .

.

, , , , , .

2% . Al2O3 0.2% 2,7 . .

1. -Al2O3

40 gr -Al2O3 75 ml H2O 250 ml. 30-45sec .

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2. : 0.25mm.

3. : 105 C .

4. : (60-80 C)

5. : 10cm.

, , . ( ), . 1.5cm . . . .

. BHT PG

, .

PG (PROPYL GALLATE)

BHA (BUTYLATED HYDROXYANISOLE)

TBHQ (TERT-BUTYLHYDROQUINONE) TERT--

BHT (BUTYLATED HYDROXYTOLUENE )

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1. Silica Gel .

40 gr Silica Gel 75 ml H2O 250 ml. 30-45sec .

2. : 0.25mm.

3. : 105 C .

4. :

5. : 12cm.

1.5% - (H3PO412MoO324H2O) . - BHT - PG.

, . . . Rf BHT PG .

13

, , 2 , , , , , .. , , , , . .

, , :

(Liquid Chromatography) . - (Liquid-Solid Chromatography), , - (Liquid-Liquid Chromatography), .

(Gas Chromatography) , o - (Gas-Solid Chromatography), - (Gas-Liquid Chromatography), .

14

,

(Adsorption Chromatography). , .

(Ion-Exchange Chromatography), .

(Partition Chromatography), .

(Molecular Exclusion Chromatography), , . .

(Affinity Chromatography) .

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4. (GC-FID)

GC

.

50-200 mg/L 1-10 % , 0.05 % v/v -1

. SHIMADZU GC-8A (FID) SHIMADZU C-R6A. 0,6 0,2 Kg/cm2 . Porapac S 2 m 1/8 . 130-180 oC, 3 oC/min. 210 oC. 60 mL/min. (2 L) .

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5. , HPLC

HPLC (High Performance Liquid Chromatography), . . GC, , , .

.

(HPLC) 1-10 % v/v (, , .., ) 1-10 % w/v, 0.1 % v/v -1 . SHIMADZU LC-9A

Shim-pack SCR-101 N, 60C, , 0.8 mL/min, (RID).

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1% v/v 0,45 m.

.

HPLC (, , , , .., ) . Jasco LC-2000 Series HPLC system (Jasco Inc., Japan) Bio-rad Aminex HPX-87H (300x 7.8 mm i.d., 9 m particle size), CO-2060 PLUS, PU-2089 pump, AS 2050 PLUS MD-2018 Photodiode rray. 50C 0.008N H2SO4 0.6mL/min. 210 nm. 20 L 0.22m.

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6. (AAS)

(Atomic Absorption Spectroscopy) . . , .

Nu , , .

Beer :

CkbNkbk434,0P/PlogA ovo

=

Po =

P = ,

kv = , , v,

k, k = ,

b = , ( , )

= ( ml)

C =

19

. , .

:

i) , .

ii) - . -.

iii) , .

iv) , .

v)

vi) , .

:

background .

.

20

Shimadzu -6500 series AAS

-6500 series AAS Shimadzu

-6501

ASC-6000 Autosampler

GFA-6000

. ( ppm = mg/l) .

. Fe, Cu Ca (1000 mg/l) (.. Cu Fe 2,5, 5, 10 20 ppm, Ca 5, 10, 40 80 ppm) (.. 11,5 ).

To Fe =248,3 nm, Ca =422,7 nm o Cu =324,8 nm.