2014年 4月研究生读书报告王凯

硕士研究生临床检验诊断学

Seminars In Cancer Biology

A review of the current understanding and clinical utility of miRNAs in esophageal cancer

Comparative Genomic Analysis of Esophageal Adenocarcinoma and Squamous Cell Carcinoma

Cancer Discovery

• Sparkle of Inspiration

• Conclusion

• Genomic Analysis of Esophageal Cancer

• miRNAs In Esophageal Cancer

1 2

34

Four miRNAs (mi-R-25, -99a, -133a and -133b) showed good potential as diagnostic markers and interestingly five (mi-R-21, -27b, -126, - 143 and -145) appeared to be useful both as diagnostic and prognostic/predictive markers.

Summary of studies looking at miRNA expression in tumor tissues and non-tumor tissues

Summary of miRNAs identified to have a role in determining prognosis

Summary of key miRNAs identified as having a potential role in the diagnosis and/or prognosis of oesophageal cancer

• Sparkle of Inspiration

• Conclusion

• Genomic Analysis of Esophageal Cancer

• miRNAs In Esophageal Cancer

1 2

34

11 EAC

23 fresh-frozen

primary tumors

NOTCH1 TP53 C:G>G:CA:T>C:G

Tumor Tissue EAC

12 ESCC

ESCC

• Sparkle of Inspiration

• Conclusion

• Genomic Analysis of Esophageal Cancer

• miRNAs In Esophageal Cancer

1 2

34

Find out the drive gene

MicroRNAs matched with the drive gene

Quantitative detection of the expression of the gene and microRNA

Analyze the relationship between the expression of the gene and microRNAs

Experimental verification

• Sparkle of Inspiration

• Conclusion

• Genomic Analysis of Esophageal Cancer

• miRNAs In Esophageal Cancer

1 2

34

Thank You