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221 and 222 XL Liquid Handlers User’s Guide

221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

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Page 1: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

221 and 222 XL Liquid HandlersUser’s Guide

Page 2: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Model 221XL Model 222XLPart Number Qty Part Number Qty Description

...27067213 1 27067213 1 Constricting needle, 316L,160.5 x 1.5 x 1.1 mm...

Model 221XL Model 222XLPart Number Part Number Description

...27067213 27067213 Constricting needle 316L, 160.5 x 1.5 x 1.1 mm...

page Appendix A-2

page Appendix A-13

Errata 221/222 XL User’s Guide

Ref : LT801483/A

Page 3: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

221 and 222 XL Liquid HandlersUser’s Guide

LT801205/H - ©2003 Gilson SAS All rights reserved April 2003

Page 4: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations
Page 5: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Table of Contents-1

Table of Contents 0

Safety

1 IntroductionUnpacking ...................................................................................... 1-2Warranty ......................................................................................... 1-3Customer Service .......................................................................... 1-4

2 DescriptionMechanical Assembly .................................................................. 2-2Pumps.............................................................................................. 2-4Electrical Assembly ...................................................................... 2-5

Electrical Control Socket ........................................................ 2-6Microswitch Settings .............................................................. 2-6

Rinsing Stations ............................................................................. 2-8

3 Installing a Liquid HandlerPower Connection/Fuses ............................................................ 3-3

Voltage Selection ..................................................................... 3-3Fuses .......................................................................................... 3-3Connecting the Power Cord .................................................. 3-3

Installing the Vertical Arm .......................................................... 3-4Needle/Liquid Level Detector ................................................... 3-5

Principle of the Liquid Level Detector ................................ 3-5Sensitivity Limits ..................................................................... 3-5Installation ................................................................................ 3-7

Gilson Sampler Controller Keypad ........................................... 3-8Pumps.............................................................................................. 3-9

402 Syringe Pump ................................................................... 3-9401 Dilutor ................................................................................ 3-9Minipuls 3 Peristaltic Pump .................................................. 3-9

4 Installing The Additional AccessoriesHeightening Kit ............................................................................. 4-2Metallic Front Tray Assembly ..................................................... 4-3

Installation ................................................................................ 4-3Levelling the Tray Platform .................................................. 4-5

Vertical Arms ................................................................................. 4-6Needles ........................................................................................... 4-7

Constricting Needles .............................................................. 4-7Teflon Needles ......................................................................... 4-7Double Needles ....................................................................... 4-7

Contents

Page 6: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Table of Contents-2

TransferPorts™.............................................................................. 4-8Installation ................................................................................ 4-8

Injection Ports ................................................................................ 4-9Installation ................................................................................ 4-9

Thermostated Racks ................................................................... 4-10Installation .............................................................................. 4-10Perforated Covers for Thermostated Racks ..................... 4-12

Universal Rack ............................................................................. 4-13Free Racks ..................................................................................... 4-14Solvent Racks ............................................................................... 4-15Needle Wiper ............................................................................... 4-16Low Pressure Valve .................................................................... 4-17

5 MaintenanceCleaning .......................................................................................... 5-2Lubrication ..................................................................................... 5-3Transportation ............................................................................... 5-4Position Alignment Check ........................................................... 5-5

AppendicesA - Parts and Accessories Lists .............................. Appendix A-1

Standard Equipment .......................................... Appendix A-2Accessories .......................................................... Appendix A-3Gilson Sample Racks ......................................... Appendix A-4FEP Tubing.......................................................... Appendix A-11Parts Lists for 221 and 222 XL Generics ....... Appendix A-12

B - GSIOC ................................................................... Appendix B-1Immediate Commands ....................................... Appendix B-2Buffered Commands ........................................... Appendix B-5

C - Technical Data .....................................................Appendix C-1Hydraulics ............................................................Appendix C-2Mechanics .............................................................Appendix C-3Electrical ................................................................Appendix C-4Control ...................................................................Appendix C-5Programs ...............................................................Appendix C-6Environmental Conditions ................................Appendix C-7

D - Reference Information ...................................... Appendix D-1Chemical Inertness of Plastic Used ................. Appendix D-2Rack Compatibility Table ................................. Appendix D-3

Page 7: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Safety-1

Safety 0

Read this section carefully before installing and operating the instrument.

For safe and correct use of the instrument, it is essential that both operating and servicepersonnel follow generally accepted safety procedures as well as the safety instructionsgiven in this document, the 221 and 222 XL Liquid Handlers User’s Guide.

The instruments described in this document are liquid handlers and should be used forautomatic liquid transfer operations only. They should only be used in the laboratory orsimilar indoor environment for analytical purposes, by qualified personnel. If aninstrument is used in a manner not specified by Gilson, the protection provided by theinstrument may be impaired.

When the vertical arm and needle have been installed, it is necessary to check that nothinghinders the movement of the instrument parts (i.e. needle and arms). Nothing should beplaced in or around the field of movement of either the horizontal or vertical arms.

Due to the fact that the needle installed on the vertical arm may contain a dangeroussubstance, you are strongly advised against interfering in the work area of theinstrument until the instrument has come to a complete stop.

Voltages present inside the instrument are potentially dangerous. If there is a problemwith the instrument, the power cable should be removed until qualified service personnelhave repaired it. This is to prevent anyone from inadvertently using the instrument,thus causing possible harm to themselves, or damage to the instrument itself.

The leakage current of this instrument is within the limits allowed by internationalsafety standards for laboratory equipment. An efficient ground connection is imperativefor the physical protection of the user.

Power supply cord reference 7080316106 is for use in France and Germany. Powersupply cord reference 7080316105 is for use in USA and Canada. For other countriescontact your local Gilson distributor. You must only use the type of fuse described inthis document: 0.5 Amp type “T” slow blow.

Safety

Page 8: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Safety-2

Safety

Adequate protection including clothing andventilation must be provided if dangerous liquids areused in the analytical work. In case of incidentalspillage, carefully wipe with a dry cloth, taking intoaccount the nature of the spilled liquid and thenecessary safety precautions.

Cleaning, dismantling, maintenance, adjustmentand repair should only be performed by personneltrained in such work, and who are aware of thepossible dangers involved. These instruments mustnot be sterilized, using an autoclave, or any othermechanical device. When you need to clean aninstrument, use one of the three following methods:

1 - a clean dry cloth,2 - a cloth dampened with water,3 - a cloth dampened with soapy water.

If a cloth dampened with soapy water is used to cleanthe instrument, only domestic soap may be used. Noother form of detergent or chemical may be used.

These electronic and hazard symbols appear on the221/222:

!

Symbol Explanation

Alternating current

PROTECTIVE CONDUCTORTERMINAL

On (Supply switch)

Off (Supply switch)

Caution, risk of electric shock

Caution (refer to User’s Guide)

I

O

~

Page 9: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

1-1

Introduction 1

This manual is intended for users of model 221 and 222 liquid handlers. Only proceduresdescribed in this manual should be carried out by the user. 221 and 222 liquid handlers aredesigned to work in a GSIOC environment. GSIOC (Gilson Serial Input Output Channel) isa communications channel which links various Gilson modules together in a system.

Liquid handlers are slave XYZ robots which can be controlled either by computer or theGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlershelp automate liquid handling operations and sample preparation. They can beconfigured as stand alone preparation devices, easy to use sample transfer instrumentsor as systems for complete automation of sample preparation and transfer.

Unpacking ............................................................................2Warranty ...............................................................................3Customer Service ................................................................4

Page 10: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

1-2

Introduction 1U

np

acki

ng Unpacking

Liquid handlers are delivered in a single boxcontaining both the instrument and its standardparts (e.g. needles, tubing, etc...). The original boxand packing assembly should be kept in case theequipment has to be returned to the factory andespecially while the instrument is under warranty.

All parts contained in the box must be checked offby the user against the packing list supplied. Thisshould be performed as soon as possible even if theinstrument is not to be put to immediate use. Thesupplier should be notified immediately of anyinconsistencies between the box contents and thepacking list and/or of damage to parts.

When unpacking your instrument:

• Remove and open the upper compartmentcontaining the standard parts (cables, needles, etc.),

• Slide the unit and packing assembly out of the box,

• Remove the locking screw,

• Remove packing assembly.

When delivered, the horizontal arm of theliquid handler is fixed in its transportationposition by means of a screw. This screwmust be removed before the unit is installed.It is located behind a plastic plug situatedon the right-hand side-panel (refer to theopposite figure). The screw should bereplaced whenever the liquid handler istransported, as failure to do so could resultin severe mechanical damage. If this screwis lost, a replacement screw has beenprovided and is situated at the top left-hand corner of the rear of the unit.

Horizontal arm

Plastic plug

Arm-locking screw

Page 11: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

1-3

Introduction 1W

arranty

Warranty

If the instrument does not appear to functioncorrectly, first verify the electrical connections arecorrect and that the instrument is switched ON.

Contact your Gilson distributor for technical adviceor an eventual service visit. Any service requiredwill be given within the warranty conditionsassured by your Gilson distributor.

Page 12: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

1-4

Introduction 1C

ust

om

er S

ervi

ce Customer Service

Gilson and its worldwide network of authorizedrepresentatives provide you with four types ofassistance: sales, technical, applications and service.

The service personnel of Gilson's representatives areable to serve you more efficiently if you provide thefollowing information:

1) The serial number and model number of theequipment involved.

2) Computer model (if used), available memory,microprocessor and operating software version(s)in operation.

3) The installation procedure you used.

4) A concise list of the symptoms.

5) A list of operating procedures and conditions youwere using when the problem arose.

6) A list of other devices connected to the systemand a system diagram showing the connections.

7) A list of other electrical connections in the room.

Page 13: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-1

Description 2

This chapter describes the different mechanical, electrical and hydraulic components ofthe liquid handlers.

Page 14: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-2

Description 2

Tray holding bar

Support bar

Vertical armNeedle holder

Carriage casting

Liquid leveldetector cable

Rinsing station

Needleguide

Horizontal arm

Mechanical Assembly

Figures show the 221 and 222 in their standardconfiguration.

To see which racks are compatible with the differentpossible configurations, refer to the table on the lastpage of this User's Guide.

Mec

han

ical

Ass

emb

ly

Page 15: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-3

Description 2M

echan

ical Assem

bly

Rinsing station

Tray rail

Support bar

Hole containing spare arm-securing screw

Power socket

6-pin FCC type RS232 socketfor sampler controller keypad

Input/outputsockets

GSIOCconnector

25-pin D-typeRS232 socketMicroswitches

Page 16: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-4

Description 2P

um

ps Pumps

The 221 and 222 units can be used with a 402Syringe Pump, 401 Dilutor or Minipuls 3 peristalticpump connected to the needle.

All three pumps are shown in the followingdiagrams.

The pump outlet should be connected to the needlevia the spiral transfer tubing (described in theinstallation section).

The Minipuls 3 cannot be used to aspirate from avial.

Minipuls 3

401 Dilutor

Single Syringe andValve Configuration

Dual Syringe andTee Junction Configuration

Page 17: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-5

Description 2E

lectrical Assem

bly

Electrical Assembly

The rear panel of the liquid handler includes:

1. One sector block containing the power socket,fuse holder and ON/OFF switch.

2. One 6-pin FCC type socket to which is connectedthe sampler controller keypad via the RS232coiled cable.

3. Four electrical control sockets- four inputs, sevenoutputs (including three relay type) and oneanalog input, one 12 V DC output and fourground connections.

4. Eight microswitches which serve to identify themachine and to activate certain functions such aschanging the baud rate, local clock, etc.

5. One GSIOC connector through which the 402Syringe Pump, 401 Dilutor or Minipuls 3peristaltic pump is connected.

6. One 25-Pin D-type RS232 socket for computerconnection.

Never connect a liquid handler to both a samplercontroller keypad and a computer simultaneously.

Page 18: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-6

Description 2E

lect

rica

l Ass

emb

ly Electrical Control Socket

Located at the rear of the unit are four electricalcontrol sockets which consist of four contact inputsand seven outputs (including three relay type outputs).They also consist of one analog input which can beconfigured to serve as a fifth contact input (refer to“Microswitch Settings”).

The signal voltage of the analog input is + or - 100mV.Use any of the cables supplied in the cable kit forelectrical connections (ref. 709910406) to coordinateany peripheral instruments with the liquid handler.

Four barrier strip connectors are provided for thispurpose.

For liquid handlers, the analog input is normallyintended for connection to a detector but may beused for other specific user applications.

Microswitch Settings

The eight micro-switches on the rear panel of theliquid handler serve two purposes. They are used toaddress the liquid handler and to activate certainfunctions such as the baud rate, local clock, etc...

Microswitch Function Up Down no

7 Clock Local clock is selected only if Local clock is always enabledRS-232 socket is being used

6 Baud speed Baud speed = 19200 bps Baud speed = 9600 bps

5 Analog Input Input is normal Input acts as 5th contact

4 Unit ID number (in binary), 16 0most significant bit

3 Unit ID 8 0

2 Unit ID 4 0

1 Unit ID 2 0

0 Unit ID number (in binary), 1 0least significant bit

ground

no nc c no nc c no nc c

ground

+

analog input/5th contact input

ground ground321

4 5 6 7 1 2 3 4

open collectoroutputs contact inputs

+12V

outputs

Page 19: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-7

Description 2E

lectrical Assem

bly

Each switch is off when up and on when down.

The unit is factory set at 10. The diagram belowrepresents microswitches which have been set at aGSIOC address of 10.

updown

7 6 5 4 3 2 1 0

Default setting

Page 20: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

2-8

Description 2R

insi

ng

Sta

tio

ns Rinsing Stations

Liquid handlers are equipped with rinsing stationssituated at the extremities of the support bar*. Forthe 221 model there is only one rinsing stationlocated on the left hand-side of the support bar. Asthe 222 model is larger, 2 rinsing stations areprovided, one at either end of the support bar. Thereason for this is to speed up the rinsing process.When the needle is left of center of the support barduring operation the needle is rinsed in the rinsingstation to the left of the bar and in the rinsing stationto the right of the bar if right of center. Although thisconfiguration is standard for a number of Gilsonapplications, the user can, if desired, program theneedle to be rinsed in one rinsing station only.Rinsing stations serve to clean the needle bothexternally and internally.

Rinsing well for level sensing

Drain

Rinsing well

Rinsingstationplug

* There are three positions formounting the rinsing stations onthe support bar: A, left side, B, theright of the center, and C on theright end of the support bar.

Positions A and C are compati-ble with older versions of 222.However, in this configuration,the right station is close to theright valve.

Position B offers better access tothe right injection valve and isrecommended as the standardinstallation for the right rinsingstation on new samplers.

The rinsing process is performed as follows:

1) A predetermined volume of rinsing solvent isflushed through the needle, removing bubblesfrom the FEP tubing and flushing any insolublematter from the needle.

This solvent is dispensed in the rinsing stationdrain.

2) The needle is then plunged into the rinsing wellcontaining the solvent, which serves to rinse theneedle externally. Another volume of rinsingsolvent is then dispensed in the rinsing well.Excess solvent in the rinsing well overflows intothe drain leading to a waste receptacle.

When the liquid level detector is activated thesmaller rinsing well is used. The user has thepossibility of removing the larger rinsing well andreplacing it with a special plug if the liquid leveldetector is to be used for long periods. This preventsthe larger rinsing well from filling up with sediment.

Whenever the standard support bar is replaced, theuser must remember to remove the rinsing stationfrom the standard support bar and install it on thereplacement bar.

Page 21: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-1

Installing a Liquid Handler 3

The liquid handler is delivered, factory-fitted with support bar, rinsing station (1 for the221 model and 2 for the 222 model) and tray holding bar (refer to the figures on the nextpage).

For safety purposes, the vertical arm is not installed but is supplied in the partscompartment of the packing. The vertical arm must be installed by the user (refer to theprocedure described in chapter “Installing the Vertical Arm”).

Page 22: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-2

Installing a Liquid Handler 3

221 XL

222 XL

Tray

Tray holding bar

Support bar

Rinsing station221 front panel

Tray

Tray rail

Support barRinsingstation

222 front panel

Page 23: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-3

Installing a Liquid Handler 3

Power Connection/Fuses

Voltage Selection

The sector block situated on the rear panel of theunit contains the ON/OFF switch, power socket andfuse holder/voltage selector.

Both the 221 and 222 models are pre-set to operateon 240V. In order to change the voltage configuration,check that the ON/OFF switch is in the OFFposition and the power cord is not connected to themachine. Then, using a small screw-driver orsimilar, pry the fuse holder open and remove it fromthe power receptacle.

The voltages, either 110/120V or 220/240V indicatedat the rear of the fuse holder can be changed bysimply turning over the fuse holder, such that thearrow corresponding to the required voltage, pointsto the alignment mark on the power receptacle body.It is important to check that the voltage selectedcorresponds to that of the power supply used.

Fuses

The instrument requires two fuses to be installed.The type of fuses required are 0.5 Amp type “T”slow blow.

For safety reasons, liquid handlers are deliveredwithout fuses installed. Fuses must be installed bythe user upon delivery.

Connecting the power cord

Make sure the ON/OFF switch is in the OFF (down)position. Connect the power cord to the powersocket at the rear of the unit and then plug in to thepower supply source. When the power cord hasbeen connected and the ON/OFF switch is put tothe ON position, a green LED on the front carriagecomes on.

Po

wer C

on

nectio

n/F

uses

Page 24: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-4

Installing a Liquid Handler 3In

stal

ling

th

e V

erti

cal A

rm Installing the Vertical Arm

Before installing or changing the vertical arm, checkthat the ON/OFF switch is in the OFF (down) positionand that the power cord has been disconnected fromthe unit’s power socket. Check also that the horizontalarm locking screw has been removed.

a) Remove the black nylon screw from the tip of thehorizontal arm by turning it counterclockwise.

b) Carefully extract the hexagonal control rod bypulling it outwards.

c) Move the slider to an intermediate position onthe horizontal arm.

d1)Insert the vertical arm into the horizontal slider.When viewed from above, the needle holdermust be on the right-hand side.

d2)Firmly secure the vertical arm by inserting thetwo screws supplied, into the underside of theslider and vertical arm.

d3)Connect the power cord and switch the ON/OFFswitch to its ON position. Wait while the motorsare reset to zero.

d4)When the arm has moved to its zero position, movethe needle holder upwards gently until it stops.

e1) Insert the hexagonal rod into the horizontal armso that it passes through the vertical arm’sgearing and into the motor drive socket.

e2) Move the needle holder downwards by about 1mm from the upper limit, at the same time gentlypush the rod until it clicks into position.

e3) Replace and tighten the black nylon screw.Switch the ON/OFF switch to its OFF position.

f) If the size of the arm installed is not the same asthe one offered by Gilson in the standardpackage, the Z-arm must be calibrated. Use theZ-arm calibration software for XL samplers, orcall the local Gilson distributor for calibration.

The assembly process must be performed in theorder specified above so that the mechanical andelectrical components of the unit are coordinated.The unit cannot operate correctly if the needleholder is significantly more than 1 mm away fromthe vertical arm’s upper limit.

This diagram shows the undersideof the horizontal arm and slider.

a)

b)

c)

d)

e)

Page 25: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-5

Installing a Liquid Handler 3N

eedle/L

iqu

id L

evel Detecto

r

Needle/Liquid Level Detector

The liquid level detector cable is an accessory. Whenit is installed, the instrument no longer conforms toEMC requirements.

A special feature of 221 and 222 Liquid Handlers isthe liquid level detector. It is used to detect when theneedle has reached the liquid surface which permitsthe instrument to calculate the change in the liquidlevel so that the tip of the needle always remains apredetermined distance below the surface. It canoperate only when the liquid handler is used inconjunction with a 402 Syringe Pump or a 401 Dilutor.It does not function when the Minipuls 3 peristalticpump is used. The purpose of the liquid leveldetector is to reduce the surface of the needlecoming into contact with the liquid thus reducingcontamination

Principle of the Liquid Level Detector

The needle descends into the tube until it reachesthe surface of the liquid. When this happens, asignal is sent to the processor. From the moment theprocessor receives this signal, it starts to calculatethe vertical speed required for the needle to followthe liquid level as it changes. The vertical speed ofthe needle is calculated as a function of the flow rateand the cross sectional area of the tube entered.Once the processor knows the rate at which the levelin the vial changes, it can adjust the speed of theneedle to remain a constant distance below the levelof the liquid.

Sensitivity Limits

The liquid level detector can only detect certainliquids which have a dielectric constant greater than18 (for a sensitivity value of 3). This means thatalmost all water-soluble solvents (i.e. aqueoussolutions, common alcohols, acetone andacetonitrile) are easily detected by the liquid leveldetector. Among the solvents which cannot bedetected are hydrocarbons such as hexane,cyclohexane and benzene and chlorinated solventssuch as chloroform and dichloromethane.

Page 26: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-6

Installing a Liquid Handler 3N

eed

le/L

iqu

id L

evel

Det

ecto

r During normal operation, the recommendedimmersion depth for the needle is 2 mm.

It is important to note that:

• The liquid level detector is not compatible withseptum piercing. The reason for this being thatwith septum piercing, the needle sleeve mustalways enter the tube to compensate for thedecrease of air pressure within the tube when theliquid is aspirated.

• The liquid level detector does not function forcode 8 racks.

• The liquid level detector only functions with code9 racks if the 402 Syringe Pump or 401 Dilutor isinstalled with either a 0.5 ml or 1 ml syringe.

• The maximum recommended aspiration flow ratesin order to avoid immersion errors, are:- 7 ml/min for small vials (in general, vials of

volume 4 ml or less),- 10 ml/min for open tubes,- 20 ml/min for 20 ml vials.

When the liquid level detector is activated, a precisevalue must be entered for the inner diameter of thevial. The vial diameter is measured in tenths ofmillimeters. The Gilson program offers a defaultvalue for each vial diameter at the programmingstage of the operation. As the rate at which theneedle descends or rises in the vial depends on theinner diameter of the vial, it is important to enter avalue of within one tenth of a millimeter. Below aresome examples of possible errors generated byentering the wrong internal tube diameter:

• For a code 21 rack, the default tube diameter is11.3 mm. If there is an error of +/- 0.1 mm enteredfor the tube diameter, then the resulting immersionerror will be +/- 1.5 mm. If there is an error of +/-0.5 mm entered for the tube diameter then theresulting immersion error is +/- 7 mm.

• For a code 9 rack, the default tube diameter is6.1 mm. If there is an error of +/- 0.5 mm enteredfor the tube diameter, then the resulting immersionerror will be +/- 4 mm.

The liquid level detector sensitivity can be adjustedby the user at the programming stage of the operation.For further information on sensitivity levels, etc.refer to the software manual.

Page 27: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-7

Installing a Liquid Handler 3N

eedle/Liquid Level Detector

Installation

To install the needle/liquid level detector:

1) Slide the needle into the tubing fitting.

2) Select the FEP (polytetrafuoroethylene) transfertubing for the unit to dilutor connection whichcorresponds to the volumes to be transferred.

3) Connect the selected tubing to the fitting.

4) Slide the needle assembly into the needle holderon the vertical arm so that it passes through theneedle guide then connect the other end of thetubing to the outlet of the 402 Syringe Pump, 401Dilutor or the Minipuls 3 Peristaltic Pump.

5) Slide the ring contact of the liquid level detectorwire into the slot provided on the needle holder.Check that the contact enters the slot fully so thatthe tightening screw passes through the ring.This is important as failure to do so could lead toliquid level detection errors.

6) Connect the liquid level detector wire to thecoaxial socket situated just above the power-onLED at the front of the carriage casting.

7) If larger diameter needles or probes such asteflon coated probes are used, you may need tomount specific needle holders and needle guidesinstead of the standard ones.

Needleguide

FEP tubing

NeedleRingcontact

Tubingfitting

Needleholder

Liquid level detector wire

Page 28: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

3-8

Installing a Liquid Handler 3G

ilso

n S

amp

ler

Co

ntr

olle

r K

eyp

ad Gilson Sampler Controller Keypad

The Liquid Handlers are available with either735 Sampler Controller Software or with the KeypadController and the 720 software.

For installation of the 735 Sampler ControllerSoftware, refer to the relevant User’s Guide.

The Sampler Controller Keypad supplied in theparts compartment of the packing assembly controlsthe liquid handler and connected pumps.

The keypad is connected to the instrument via anRS232 coiled cable. Characters are displayed inwhite on a blue background.

The keypad consists of:

- an eight-line, 40 character visual display unit,

- five menu soft-touch keys just below the displayunit,

- a numerical keypad with an ENTER soft-touch key,

- four cursor direction soft-touch keys,

- a group of three function soft-touch keys , HELP,ESC., CLEAR.

- a 3.5" diskette drive with protective cover.

Type of diskettes used are 3.5" high density diskettes.

For information on using the keypad refer to thesoftware manual.

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3-9

Installing a Liquid Handler 3P

um

ps

Pumps

Liquid handlers can operate in conjunction with a402 Syringe Pump, 401 Dilutor or a Gilson Minipuls 3Peristaltic Pump.

402 Syringe Pump

The 402 Syringe Pump is a slave pump and isconnected to either the 221 or 222 Liquid Handlervia a GSIOC (Gilson Serial Input/Output Channel)connector located on the rear panel of the 402Syringe Pump. Refer to the 402 User's Guide.

401 Dilutor

The 401 Dilutor is a slave pump and is connected toeither the 221 or 222 Liquid Handler via a GSIOC(Gilson Serial Input/Output Channel) connectorlocated on the rear panel of the 401 Dilutor. Refer tothe 401 User’s Guide.

Minipuls 3 Peristaltic Pump

The Minipuls 3 Peristaltic Pump is connected to 221and 222 Liquid Handlers via a GSIOC connector.

It is important to note that the liquid level detectordoes not function with a Minipuls 3 Peristaltic Pump,and that you cannot aspirate with the Minipuls 3.Refer to the Minipuls 3 Peristaltic Pump User's Guide.

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3-10

Installing a Liquid Handler 3

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4-1

Installing the Additional Accessories 4

The reference and descriptions of the additional accessories are available in Appendix A,“Accessories” .

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4-2

Installing the Additional Accessories 4

Heightening kit

This kit is used to heighten the unit so that longertubes may be used. When this kit is used, a 183 mmstroke arm must be installed. Using this kit meansthat racks are used in the lower position. When theheightening kit is installed, racks of codes 22, 33 and34 should be entered as 92, 93 and 94 respectively.

It is available for Model 222 Liquid Handlers only. Itcontains 4 heightening legs and two tray railsupport bars. The legs are fitted to the base of theliquid handler. To install these legs simply place theliquid handler on its side and locate the four holessituated at each corner of the base of the unit. Oncelocated, simply screw-in the four heightening legsand redress the unit. The two tray rail support barsare fitted to the front panel of the liquid handlerusing the four screws supplied. The tray rail is fittedto the tray rail support bars via the hole situatednear the base of each tray rail support bar.

Hei

gh

ten

ing

Kit

Tray rail

Heightening legs

Tray rail support bar

Support bar

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4-3

Installing the Additional Accessories 4M

etallic Fro

nt Tray A

ssemb

ly

Metallic Front Tray Assembly

The metallic front tray assembly is a heighteningand support kit which is available for 222 LiquidHandlers only. It is used for certain applications (i.e.pathological tissue staining, glue dropping, etc.)where it is necessary to have a flat working surface.

The metallic front tray assembly consists of:

- a tray platform,- two platform support arms,- two adjustable supports,- four heightening legs (65 mm).

Installation

The user may install the tray platform in either theupper or lower position, depending on the application,and on the length of the vertical arm installed on theliquid handler (lower position for the 183 mm arm).

Four screws, with black plastic knurled heads, areprovided for the purpose of attaching the trayplatform to the platform support arms. The trayplatform is fixed either above the platform supportarms for the 'upper' position, or below the platformsupport arms with the tray rail uppermost andnearest to the 222 for the 'lower' position.

Platformsupportarm

Support bar

Tray platform

Tray rail

Adjustable support

Heightening legs

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4-4

Installing the Additional Accessories 4M

etal

lic F

ron

t Tra

y A

ssem

bly To install the legs simply place the liquid handler on

its side and locate the four holes situated at eachcorner of the base of the unit. Once located, simplyscrew-in the four heightening legs and redress theunit. The two metallic platform support arms arefitted to the front panel of the unit using the fourscrews supplied.

When a 183 mm vertical arm is used the trayplatform must be in the lower position. This is toavoid damage to the needle during operation.

When a 123 mm vertical arm is used, the trayplatform must be in the upper position.

Installing the Platform in the Upper Position

To install the tray platform simply:

1) Remove the two plastic headed screws from thetop of the support arm.

2) Place the platform on the arm, with the tray railuppermost and nearest the arm angle. Replacethe two screws by passing them through the twocorresponding holes in the platform. Secure themmanually.

3) Repeat the procedure for the other arm.

4) Install the platform and arm assembly onto thefront panel of the machine using the two screwsprovided on each arm.

Installing the platform in the lower position

For some applications (notably, when using the 183 mmstroke arm) it is necessary to install the platform in itslower position, beneath the support arms as follows:

1) Remove the two plastic headed screws and theadjustable support from the support arm.

2) Position the platform beneath the support armwith the tray rail uppermost and nearest theangle. (i.e. the tray rail towards the machinewhen the assembly is installed).

3) Locate the two smaller holes in the base of thesupport arm and position the arm such that theseholes correspond to the two smaller holes in theplatform.

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4-5

Installing the Additional Accessories 4M

etallic Fro

nt Tray A

ssemb

ly

4) Secure the platform to the arm from beneathusing the two plastic headed screws.

5) Locate the third hole in the base of the platformand insert the adjustable support.

6) Repeat the procedure for the other arm.

7) Install the platform and arm assembly onto thefront panel of the machine using the two screwsprovided on the arm.

Levelling the tray platform

The tray platform must be horizontal for correctneedle operation. Two adjustable supports areprovided on the underside of each support arm forthis purpose.

Adjustment is performed as follows:

1) Manually loosen the support’s locking collarslocated at the top of each support, by turningthem clockwise.

2) Turn the foot of each support to level the trayplatform. Check, using a spirit level, that theworkbench, liquid handler and platform arelevel. However, this adjustment is not so criticalas to require an absolutely level reference surface.

3) Manually tighten the support’s locking collars, byturning them counterclockwise (when viewedfrom above).

Installing the rack-tray

Place the rack-tray on top of the tray platform withthe groove on the underside of the rack-tray firmlyslotted over the tray rail.

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4-6

Installing the Additional Accessories 4V

erti

cal A

rms Vertical Arms

The installation procedure for the long and shortarms and needle kits is as described in Chapter 3.When the user has changed the size of the verticalstroke arm, it is necessary to call the local Gilsondistributor for recalibration of the machine as themachine will take the arm size as being 123 mm bydefault.

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4-7

Installing the Additional Accessories 4N

eedles

Needles

Various needle types are available for use with both221 and 222 Models. They are:

Constricting needles

When using TransferPorts™, beveled edge constrictingneedles must be used.

These needles are specifically designed for dealingwith small volumes. They have a specially designedtip which reduces the risk of cross contaminationbetween samples and increases measuring accuracywhen dealing with small volumes.

Refer to Chapter 3 for installation.

Teflon needles

Teflon needles are chemically inert needles. They areused for applications where metal traces such asstainless steel and titanium, from which needles arenormally made, should be avoided.

Refer to Chapter 3 for installation.

Double needles

Double needles consist of both an inner and an outertube which act as two separate needles. They caneither be used to mix two different samples in thesame vial or for sample agitation or evaporation.Sample agitation and evaporation is performed bysending a gas into the vial through either the inneror outer tube and the sample through the remainingtube. The gas creates bubbles which agitate thesample.

Refer to the instructions leaflet supplied with thedouble needle kit for installation.

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4-8

Installing the Additional Accessories 4Tr

ansf

erP

ort

s™ TransferPorts™

In some configurations, TransferPorts may be usedto aspirate large volumes of either a sample or asolvent. The 221 has 8 possible positions and the 222has 15 possible TransferPort positions.

As TransferPorts are an additional accessory, it isnecessary to replace the standard support bar withthe specific TransferPort holding bar (ref. 2949192for 221 and 2954706 for 222) which consists of aseries of numbered holes. To change the standardsupport bar simply unscrew the knurled screwssecuring it and replace it with the transfer portholding bar. A bevelled edge constricting needlemust be installed by the user if TransferPorts are tobe used.

Installation

TransferPorts are installed as follows:

1) Place the TransferPort in the appropriatemounting hole on the transfer port holding barand screw the TransferPort and tubing connectortogether.

2) Fit a yellow cap to the top of the TransferPort.

3) Connect the other end of the male tubing to afilter, and place it in a solvent or sample bottle.

4) Fix the plastic clip onto the TransferPort.

1) Users are advised to place thebottles containing the sampleswhich are to be aspiratedthrough the TransferPorts, atthe same level or higher, thanthe rack tray.

2) When working with Transfer-Ports, the user must always usea bevelled edge constrictingneedle.

3) TransferPorts must be primedusing the program beforebeing used.

TransferPort is a trademark ofGilson.

TransferPort holding bar

Plasticcap

Yellow cap

Connector

Connectortubing

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4-9

Installing the Additional Accessories 4In

jection

Po

rts

Injection Ports

When a sample is prepared, it can be injected into aperipheral instrument via an injection port whichcan be installed on the support bar.

Installation

The injection port is installed in the hole supplied tothe right hand side of the standard support bar asfollows:

1) Separate the upper part of the injection port,(with hexagonal head) from the main body byunscrewing it.

2) Insert the upper part of the injection port into thespecific hole to the right of the support bar andscrew in the main body from beneath.

When the seal (2954674) is worn, simply extract itusing a needle or similar and replace it.

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4-10

Installing the Additional Accessories 4

Thermostated Racks

Thermostated racks consist of an aluminium rackand a thermostating cuvette. There are two types ofthermostating cuvette: one regulated by a circulatingfluid, and the other regulated by Peltier effect cells,electronically controlled by the Gilson 832 TemperatureRegulator.

They are used to regulate the sample temperature,e.g. cooling for the preservation of biological samplesand moderate heating for chemical derivatization.Possible temperatures range from 4 to 60°C for thefluid regulated cuvette and 4 to 40°C for the peltiereffect thermostating cuvette.

• The fluid regulated cuvette is available underreference 2704429.

• The Peltier effect thermostating cuvette is availableunder reference 2759550 and must be used inconjunction with the Gilson 832 TemperatureRegulator, reference 2759510.

Installation

The rack code is the number by which the rack isknown to the software. This is extremely importantto remember at the programming stage of theapplication. Refer to Appendix A for the correct codenumber, reference number and correspondingposition of all racks. The unperforated rack must bedeclared as a custom rack.

Th

erm

ost

ated

Rac

ks

When the aluminum thermostatingracks are used on the 222, some ofthem can be placed differentpositions. As a result, theidentification code used for therack changes so that the differentrack and vial coordinates can berecognized by the software.

Rack code Actual code Position Equipment requiredfor software on rack

30 (30) Normal Metallic Holder (2704473) and thermostating cuvette.

31 (31) Normal Metallic Holder (2704473) and thermostating cuvette.

32 (32) Normal Metallic Holder (2704473) and thermostating cuvette.

33 (33) Normal Metallic Holder (2704473) and thermostating cuvette.

34 (34) Normal Metallic Holder (2704473) and thermostating cuvette.

93 (33) Low* Heightening kit for sampler and thermostating cuvette.

94 (34) Low* Heightening kit for sampler and thermostating cuvette.

Custom rack no code Normal Metallic Holder (2704473) and thermostating cuvette.This rack (2704499) is unperforated (a free rack) andmust be drilled according to your requirements, thendeclared in the PC software tray configuration.

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4-11

Installing the Additional Accessories 4

Installing a Thermostated Rack on the 221 XL

1) Remove the standard support bar and install thespecific thermostated rack holding bar (reference2704471M).

2) Install the rack in the thermostating cuvette.

3) Position the cuvette containing the rack, on therack holding bar.

4) Connect the hydraulic connections/connect thecontrol cable to the 832 if the thermostatingcuvette is the Peltier effect type.

Installing a Thermostated Rack on the 222 XL

1) For the racks installed on the tray, install theheightening kit on the sampler (reference 2749870).Follow the instructions supplied with the kit.

2) For the racks in the normal position, replace thestandard support bar with the rack holding barsupplied in the rack holder kit (reference 2704473).Fix the rack holder to the tray rail following theinstruction supplied with the kit.

3) Loosen the two nylon screws on the front panelof the rack holder.

4) Tilt the front panel forwards.

5) Place the rack inside the thermostating cuvette.

Th

ermo

stated R

acks

* Racks in the normal positionare placed in the thermostatingcuvette and mounted on themetallic rack holding bar.Racks in the low position areplaced in the thermostatingcuvette and mounted on thetray. The sampler is in theraised position using theheightening kit, reference2749870.

When installing a thermostatedrack on the 222 model, there aretwo positions:

•'normal' for a rack installed onthe rack holding bar (kitreference 2704473)

•'low' for a rack installed on thetray the sampler must be fittedwith a heightening kit (kitreference 2749870).

Thermostated rack holderRack holding bar suppliedin the rack holder kit

Tray rail (installed on instrument)

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4-12

Installing the Additional Accessories 4

6) Install the cuvette in one of the five possiblepositions. Each position is marked by twoopposite pins which serve to locate and securethe cuvette. One pin is situated on the holdingbar, the other at the rear of the front panel of therack holder. Position the notches on thepolypropylene cuvette over the locating pins.

7) Tighten the two black nylon screws on the frontpanel of the rack holder.

8) Connect the hydraulic connections/connect thecontrol cable to the 832 if the thermostatingcuvette is the Peltier effect type.

Perforated Covers for ThermostatedRacks

When thermostated racks are used to cool samplesbelow the ambient temperature, condensation formson the surface of the rack and falls into the rackwells. This water accumulates in the wells andforces the vials in the rack to rise. If this happens,needle movement may be obstructed and the needledamaged. For this reason stainless steel perforatedcovers have to be installed. They are clipped ontothe rack handles as shown. These covers areavailable for rack codes 30A, 31A, 33A and 34A.

Below a list of the perforated covers available.

Th

erm

ost

ated

Rac

ks

Reference Description

27044307 30A rack cover, stainless steel, 60 holes

27044317 31A rack cover, stainless steel, 108 holes

27044337 33A rack cover, stainless steel, 14 holes

27044347 34A rack cover, stainless steel, 36 holes

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4-13

Installing the Additional Accessories 4U

niversal R

ack

Universal Rack

Universal racks (code 22U) are designed toreceive 44 vessels of varying sizes. The sizesof these vessels range from 10 mm to 18 mmexternal diameter and from 100 to 180 mm inlength. Each of the reception cavities contains4 positioning and retaining clips.

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4-14

Installing the Additional Accessories 4

Free Racks

Free racks are blank racks with no vial positionsdrilled. These racks can only be used with softwaresthat offer free rack programming (custom rackoption), essentially PC based softwares (735 and719).

Reference 2704599 is a free rack in polypropylene.

Reference 2704499 is a free thermostating rack inaluminum.

Drill the vial positions according to your methodrequirements, then declare the coordinates in thesoftware configuration.

Fre

e R

acks

2704599

2704499

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4-15

Installing the Additional Accessories 4

Solvent Racks

Solvent racks code 60 (reference 2954651) and 61(reference 2954715) hold up to four solvent bottles.Gilson supplies two types of solvent bottles, 250 mlpolypropylene bottles and 180 ml glass bottles.

Select bottles which are comecure the bottles inposition and ensure that the needle penetrates eachbottle cap correctly.

When using rack code 61, a 183 mm vertical strokearm, corresponding needle and the heightening kitmust be installed.

So

lvent R

acks

Code 61

Code 60

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4-16

Installing the Additional Accessories 4

Needle wiper

Needle wipers are used to reduce contaminationrisks when using rack codes 20, 21, 28 or 29.

They wipe excess liquid from the exterior of theneedle as it is being withdrawn from the sample.

Needle wipers consist of three perforated plasticplates between which are held two sheets ofabsorbent paper. Refer to the opposite figures for theneedle wiper assembly.

Nee

dle

Wip

er

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4-17

Installing the Additional Accessories 4L

ow

Pressu

re Valve

Low Pressure Valve

The low pressure valve is an electronically operated(12 VDC) 3-port, 2-position valve and is used for thepurpose of evaporating a solvent with an inert gasor mixing liquids contained in a vial by permittingthe passage of air into the liquids (the MIX by airmethod - refer to the software User’s Guide.

The valve has three connections: two inlets, anormally closed (NC) inlet, and a normally open(NO) inlet; and one common (COM) outlet. This isthe default position of an unpowered valve. Thevalve receives its power from the connector on thecarriage casting.

Installation

1) Remove the screw situated on the upper left handcorner of the front panel of the vertical arm.

2) Slide the valve attachment piece onto the frontpanel of the vertical arm until the hole at the topleft hand corner of the attachment piececorresponds to the hole left in the arm by theremoval of the screw.

3) Fix the valve to the vertical arm using theattachment screw supplied.

4) Connect the 402 Syringe Pump, 401 Dilutor orMinipuls 3 Peristaltic Pump to the NO (normallyopen) inlet port of the valve.

5) Connect the FEP tubing, (already connected tothe needle assembly), to the valve outlet port.

6) Connect the air supply (or inert gas or alternativefluid) to the valve via the NC (normally closed)inlet port.

7) Once hydraulic and pneumatic connections havebeen made, the valve can be connected to the12 VDC output situated just below the power-onLED at the front of the carriage casting.

The maximum allowable workingpressure of the valve is 0.138 MPa.

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4-18

Installing the Additional Accessories 4

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5-1

Maintenance 5

The ON/OFF switch must always be switched to OFF and the unitdisconnected from the mains whenever a maintenance operation is to beperformed.

Only maintenance operations described here should be carried out by theuser. Other maintenance operations not described here should be referredto qualified service personnel.

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5-2

Maintenance 5C

lean

ing Cleaning

Cleaning, dismantling, maintenance, adjustmentand repair should only be performed by personneltrained in such work, and who are aware of thepossible dangers involved.

These instruments must not be sterilized, using anautoclave, or any other mechanical device. Whenyou need to clean an instrument, use one of thethree following methods:

1) A clean dry cloth.2) A cloth dampened with water.3) A cloth dampened with soapy water.

If a cloth dampened with soapy water is used to cleanthe instrument, only domestic soap may be used.No other form of detergent or chemical may be used.

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5-3

Maintenance 5L

ub

rication

Lubrication

Model 221 and 222 Liquid Handlers should never belubricated. The user is strongly advised againstoiling or greasing any moving part such as guidingrods, gears, belts etc.

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5-4

Maintenance 5Tr

ansp

ort

atio

n Transportation

If the liquid handler is to be transported, the user isadvised to remove the vertical arm and replace thehorizontal arm locking screw. Failure to do so couldresult in misalignment or belt breakage.

When transporting over shorter distances (i.e.within the laboratory etc...) the liquid handler maybe carried by its support bar.

Liquid handlers must, on no account, be picked upor carried by either their vertical or horizontal armsas belt breakage or misalignment could occur.

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5-5

Maintenance 5P

ositio

n A

lign

men

t Ch

eck

Position Alignment Check

Liquid handlers leave the factory perfectly alignedand do not normally require checking. As previouslymentioned, misalignment may be caused bytransporting the unit without having previouslysecured the horizontal arm or by carrying the unitby its arms. If misalignment should occur, it ispossible to check alignment by examining theplacement of the needle in the rack position. If thereis any doubt about alignment call service.in the recess using a soft cloth or an air line.

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5-6

Maintenance 5

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Appendix A-1

Parts & Accessories Lists Appendix A

Parts lists for the 221 and 222 XL Liquid Handlers, consisting of Standard equipment,Accessories, Racks and Spare parts.

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Appendix A-2

Parts & Accessories Lists Appendix AS

tan

dar

d E

qu

ipm

ent Standard Equipment

Model 221XL Model 222XLPart Number Qty Part Number Qty Description

270021 221XL Liquid Handler, 110-220 V271021735 221XL Liquid Handler with 735 Software, 110-220 V

271052 222XL Liquid Handler, 110-220 V271052735 222XL Liquid Handler with 735 Software, 110-220V

21067720 21067720 Gilson 720 sampler controller software package(only with 270021 and 271052)

27072001 1 27072001 1 Needle tubing fittingF1410153 1 F1410153 1 PVDF union connector for 1/4” - 28 TPI fittings49967202 1 49967202 1 FEP tubing, 500 x 2.5 x 1.5 mm, 800 µl49967392 1 49967392 1 FEP tubing, 1000 x 2.5 x 1.5 mm, 1600 µl27067211 1 27067211 1 Bevelled edge needle, 316L, 160.5 x 1.5 x 1.1 mm27077213 1 27077213 1 Constricting needle, 316L,160.5 x 1.5 x 1.1 mm4701177592 1 4701177592 2 Rinsing station drain tubing, 1000 x 6.5 x 4 mm27062634 1 27062634 1 Needle sleeve holder, polyacetal, 2.1 mm hole with screws2707255 1 2707255 1 Rinsing station plug

54081252 3 54081252 3 Adhesive retainer for transfer tubing1704731 1 15074002 1 Polypropylene tray270620 1 270620 1 123 mm stroke vertical arm

270230 1 270230 1 Sampler controller keypad with RS 232 coiled cable36078143 1 36078143 1 GSIOC cable for 22X to 401, 402 or Minipuls connection360831221 1 360831221 1 RS232 cable for connecting sampler to PC52903071 1 52903071 1 Self-adhesive label for GSIOC identification638306512 4 638306512 4 Barrier strip connectors, 6-pin for electrical control socket2749592 1 2749892 1 Liquid level detector wire (400 mm for 221, 480 mm for 222)7080316105 1 7080316105 1 Power cord - 100-120 V, 50-60 Hz7080316106 1 7080316106 1 Power cord - 220-240 V, 50-60 Hz6730054006 5 6730054006 5 Fuses 0.5 Amp type “T” slow blow

LT801205 1 LT801205 1 221 and 222 Liquid Handlers User’s Guide

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Appendix A-3

Parts & Accessories Lists Appendix AA

ccessories

Accessories

Arms and needle related items

With 221 and 222 Liquid Handlers, there is thepossibility of replacing the standard 123 mm strokevertical arm with either a 56 mm or 183 mm strokearm.

The 56 mm stroke arm is used for racks in the upperposition containing vials with septa.

The 183 mm stroke arm is for use with longer tubes.

Below is a list of the various accessories which canbe used in conjunction with each of the three arms:

Model 221XL Model 222XLPart Number Part Number Description

For the 56 mm stroke arm270610 270610 56 mm stroke vertical arm27067271 27067271 Bevelled edge constricting needle, for use with TransferPorts,

316L, 93.5 x 1.5 x 1.1 mm27077113 27077113 Constricting needle, 316L, 93.5 x 1.5 x 1.1 mm

For the 123 mm stroke arm27067212 27067212 Side aperture needle for septum piercing and/or liquid mixing,

316L, 160.5 x 1.5 x 1.1 mm27067276 27067276 Flat tipped needle for drop transfer, 316L, 165 x 0.7 x 0.4 mm27067277 27067277 Needle sleeve for 27067276, 155 mm, flat tipped without “V”-cut27367513 27367513 Teflon needle kit for applications where contact with metal is

undesirable (contains: Teflon needle, 157.5 x 0.9 i.d. ; Special needleholder ; Needle guide)

27167215 27167215 Double needle kit (contains: Flat tipped needle, 181.5 x 1.5 x 1.1 mm ;Triangular flat tipped needle, 169.5 x 2.5 x 1.1 mm ; PVDF connector ;Special needle holder ; Needle guide)

For the 183 mm stroke arm270630 183 mm stroke vertical arm27067361 Bevelled edge needle, 316L, 220.5 x 1.5 x 1.1 mm27067373 Constricting needle, 316L, 220.5 x 1.5 x 1.1 mm27067374 Bevelled edge constricting needle, for use with TransferPorts™,

316L, 220.5 x 1.5 x 1.1 mm27067362 Side aperture needle, for septum piercing and/or liquid mixing

316L, 220.5 x 1.5 x 1.1 mm27367423 Teflon needle for applications where contact with metal is

undesirable (contains: Teflon needle, 220.5 x 0.9 mm i.d. ; Specialneedle holder ; Needle guide)

2749870 222 Heightening kit (contains: 4 heightening legs, 65 mm ; 2 tray railsupport bars)

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Appendix A-4

Parts & Accessories Lists Appendix AA

cces

sori

es Other Accessories

Model 221XL Model 222XLPart Number Part Number Description

2749767 2749767 Injection port2949192 2954706 TransferPort holding bar (221: 8 tubes, 222: 16 holes)2954709 2954709 TransferPort accessory kit (contains: Polyamide transfer port ;

2800 µl, FEP tubing, 1000 x 3 x 2 mm, fitted with filter 316L, 40 µm ;Sealing caps)

130742 2707404 Holding bar for racks series 0 to 102749872 Metallic front tray assembly (contains: Tray platform and arms

reference 290410 ; 4 heightening legs, 65 mm)150478 150478 Needle wiper for code 20 and 28 racks150471 150471 Needle wiper for code 21 and 29 racks15047110 15047110 Sheets of wiping paper (pack of 500)36077240 36077240 3 port, low pressure valve kit

49963242 49963242 120 µl FEP tubing (600 x 1.6 x 0.5 mm)49962392 49962392 440 µl FEP tubing (100 x 1.6 x 0.8 mm)49967202 49967202 800 µl FEP tubing (500 x 2.5 x 1.5 mm)49967392 49967392 1600 µl FEP tubing (1000 x 2.5 x 1.5 mm)49948392 49948392 2800 µl FEP tubing (1000 x 3 x 2 mm)2954583 2954583 1.1 ml coiled FEP tubing (2200 x 1.6 x 0.8 mm)2954671 2954671 3 ml coiled FEP tubing (1.5 mm i.d.)29425066 29425066 5 ml coiled FEP tubing (1.5 mm i.d)2954672 2954672 10 ml coiled FEP tubing (1.5 mm i.d.)

709910406 709910406 Cable kit for electrical connections (4 bare-ended wires 1.7 m)638306512 638306512 6-pin terminal block for electrical connection

2704471M Rack holding bar for one thermostating cuvette2704473 Rack holder kit for thermostating cuvettes and series 30

polypropylene racks13040103 Guiding bar for rack code 127044307 27044307 30A rack cover, stainless steel, 60 holes27044317 27044317 31A rack cover, stainless steel, 108 holes27044337 27044337 33A rack cover, stainless steel, 14 holes27044347 27044347 34A rack cover, stainless steel, 36 holes2954662 2954662 4 x 250 ml polypropylene solvent bottles2954663 2954663 2 x 180 ml glass solvent bottles2954682 2954682 4 glass bottle centering clips2954668 2954668 24 polyethylene cap assemblies for solvent bottles2954674 2954674 FEP injection port seal2707253 2707253 Polypropylene rinsing well (85 mm)2707255 2707255 Rinsing station plug

Page 59: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-5

Parts & Accessories Lists Appendix AG

ilson

Sam

ple R

acks

Gilson Sample Racks

Part Number Code Material Max Nb Capacity and type of vials or tubes

270430 0 PP 80 2 ml glass vial12040302 0 PP 80 1.5 ml Eppendorf plastic vial, 11 x 40 mm without lids12240101 1 PP 80 9 ml glass tube, 13 x 100 mm2707401 7 POM 96 Standard disposable microtitration plate270438 8 PP 120 0.3 ml glass vial270439 9 PP 120 0.7 ml glass vial130411 10 PP 27 5.5 ml glass tube, 12 x 65 mm130411K 10 PVDF 27 5.5 ml glass tube, 12 x 65 mm

150425 20 PP 108 4.5 ml glass tube, 10 x 100 mm150422 21 PP 60 9 ml glass tube, 13 x 100 mm150424 22, 92 PP 44 32 or 25 ml glass tube, 18 x 180* or 150 mm150498 22U PP 44 Variable size tube: 10 mm to 18* mm diameter,

stainless steel, 100* mm to 180 mm height.150426 23 PP 44 8 ml glass or plastic scintillation minivial,17 x 65 mm150427 24 PP 14 20 ml glass or plastic scintillation vial, 28 x 60 mm150420 28 PP 108 3 or 3.5 ml glass tube, 10 x 65 or 75* mm150429 29 PP 60 5.5 ml glass tube, 12 x 65 mm, 6 ml, 12 x 75* mm

2704430 30 Al 60 2 ml glass or plastic scintillation vial, 12 x 32 mm2704530P 30 PP 60 2 ml glass or plastic scintillation vial, 12 x 32 mm2704431 31 Al 108 0.7 ml minivial, 7 x 40 mm2704531P 31 PP 108 0.7 ml minivial, 7 x 40 mm2704432 32 Al 60 5.5 ml glass tube, 12 x 65* mm, 6 ml, 13 x 75 mm or

9 ml, 13 x 100 mm2704433 33, 93 Al 14 20 ml glass or plastic scintillation vial, 28 x 60 mm2704533P 33 PP 14 20 ml glass or plastic scintillation vial, 28 x 60 mm2704434 34, 94 Al 36 4 ml vial, 15 x 45 mm2704534P 34 PP 36 4 ml vial, 15 x 45 mm

2954651 60 SS 4 250 ml polypropylene or 180 ml glass solvent bottle2954715 61 SS 4 250 ml polypropylene or 180 ml glass solvent bottle

2704599 -- PP variable variable, unperforated free rack for use with PCsoftware

2704499 -- Al variable variable, unperforated thermostating free rack foruse with PC software

PP= Polypropylene;Al= Aluminum;PVDF= Polyvinylydene fluoride;POM= Polyacetal

* Only this size of tube can be used with level detection.

1) Racks of codes 20, 21, 28 and 29 are equipped to receive the needle wiper.

2) Racks of codes 92, 93 and 94 for the 222 model only are physically identical to racks of codes 22,33 and 34 respectively. These codes are used to define the position in which the rack is to beused. Codes 22, 33 and 34 are for the normal position and 92, 93 and 94 are for the lowerposition when used with a 183 mm stroke arm and heightening kit.

Page 60: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-6

Parts & Accessories Lists Appendix AG

ilso

n S

amp

le R

acks

Code 1 rack12240101

Code 0 rack270430

Code 0 rack12040302

Code 7 rack2707401

Code 8 rack270438

Code 9 rack270439

Code 10 rack(13 + 14 = 27)130411130411K

Code 20 rack150425

Code 21 rack150422

Code 22 rack150424

Code 23 rack150426

Code 24 rack150427

Code 28 rack150420

Code 29 rack150429

Page 61: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-7

Parts & Accessories Lists Appendix AG

ilson

Sam

ple R

acks

Code 30 rack(4 x 15 = 60)2704430

Code 31 rack(6 x 18 = 108)2704431

Code 32 rack(4 x 15 = 60)2704432

Code 33 rack(2 x 7 = 14)2704433

Code 34 rack(3 x 12 = 36)2704434

UnperforatedAluminium rack2704499

Code 30 polypropylene rack(4 x 15 = 60)2704530P

Code 31 polypropylene rack(6 x 18 = 108)2704531P

Code 33 polypropylene rack(2 x 7 = 14)2704533P

Code 34 polypropylene rack(3 x 12 = 36)2704534P

Unperforated polypropylene rack2704599

Code 60 solvent rack2954651

Code 61 solvent rack2954715

Page 62: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-8

Parts & Accessories Lists Appendix AG

ilso

n S

amp

le R

acks

Code 80Microtitration plate tray2749662

2749666

Left-hand tray supportfor small sampler

Right-hand tray supportfor small sampler

Tray supports for large sampler2749893

Gilson Racks and Required Accessories

221 Model

Rack Part Number Accessories required for rack use Position*code

0 270430 Rack holding bar (130742) for rack series 0 to 10 H12040302

1 12240101 Guiding bar (13040103) N

7 2707401** Rack holding bar (130742) for racks series 0 to 10 H

8 270438 Rack holding bar (130742) for racks series 0 to 10 H

9 270439 Rack holding bar (130742) for racks series 0 to 10 H

10 130411 Rack holding bar (130742) for racks series 0 to 10 H130411K

* H: higher positionN: normal position (PP tray)

**: 2707401 plate holder only.

Page 63: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-9

Parts & Accessories Lists Appendix AG

ilson

Sam

ple R

acks

Rack Part Number Accessories required for rack use Position*code

20 150425 N21 150422 N22 150424 N

22U 150498 N23 150426 N24 150427 N28 150420 N29 150429 N

30A 270443031A 270443132A 2704432 Rack holding bar (2704471M) and N33A 2704433 one thermostating cuvette (2704429)34A 2704434

30P 2704530P31P 2704531P Rack holding bar (2704471M) H33P 2704533P34P 2704534P

62 2954962 Rack holding bar (2704471M) H

80 2749662 Tray supports (2749666) for small sampler H

Free P 2704599 Free rack, polypropylene, unperforated, for use withPC softwares only, no code.

Free A 2704499 Free rack, aluminum for thermostating cuvette,unperforated, for use with PC softwares only, no code.

Free 20 150499 Free series 20 tube rack, polypropylene, unperforated Nfor use in polypropylene tray with PC software only.

* H: higher positionN: normal position (PP tray)

**: 2707401 plate holder only.

Page 64: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-10

Parts & Accessories Lists Appendix A

222 Model

Rack Part Number Accessories required for rack use Position*code

0 27043012040302

7 2707401**8 270438 Rack holding bar (130742) for racks series 0 to 10 H9 27043910 130411

130411K

20 15042521 15042222 150424

22U 15049823 150426 N24 15042728 15042029 150429

30A 270443031A 270443132A 2704432 Rack holder kit (2704473) and H33A 2704433 thermostating cuvette (2704429 or 2759550***)34A 2704434

30P 2704530P31P 2704531P Rack holder kit (2704473) H33P 2704533P34P 2704534P

60 2954651 N

61 2954715 Heightening kit (2749870) (183 mm stroke vertical arm) L

62 2954692 Rack holding bar (2704471M) for one thermostating cuvette L

80 2749662 Tray supports (2749893) for large sampler H

93 2704433 (33A) Heightening kit (2749870), thermostating cuvette*** L94 2704434 (34A) L

none 2704599 Free rack, polypropylene, unperforated for use withPC softwares only as a custom rack, no code.

none 2704499 Free rack, aluminum for thermostating cuvette***, unperforated,for use with PC softwares only as a custom rack, no code.

* H: higher positionN: Normal position (PP tray)L: lower position (Heightening kit used)

** 2707401 microtitration plate holder only.

*** 2704429 is the fluid controller thermostating rack. 2759550 is the Peltier Effect thermostatingcuvette and must be used with the Gilson Model 832 Temperature Regulator reference 2759510.

Gils

on

Sam

ple

Rac

ks

Page 65: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-11

Parts & Accessories Lists Appendix AF

EP

Tub

ing

FEP Tubing

FEP (polytetrafluoroethylene) tubing with twoend fittings to be connected to the injectionport:

Part Number i.d. o.d. Length(mm) (mm) (mm)

49964242 0.30 1.6 500499671181 0.30 1.6 300049963242 0.50 1.6 60049943392 0.50 1.6 1000499625021 0.80 1.6 50049962392 0.80 1.6 100049948202 2.00 3.0 50049948392 2.00 3.0 1000

Page 66: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-12

Parts & Accessories Lists Appendix AP

arts

Lis

ts f

or

221

and

222

XL

Gen

eric

s Parts Lists for 221 and 222XLGenerics

221XL Generic and 222XL Generic liquid handlersare similar to 221 and 222 as far as the basic structureis concerned. Accessories have been adapted tospecific customer’s requirements, and therefore varyfrom one machine to another.

Depending on the accessories, it is up to the user tosee whether any part of chapter 3 (installation)applies.

Standard Equipment

Model 221XL Model 222XLPart Number Qty Part Number Description

Hydraulic27072001 1 27072001 Needle tubing fittingF1410153 1 F1410153 PVDF union connector for 1/4” - 28 TPI fittings49967202 1 49967202 FEP tubing 500 x 2.5 x 1.5 mm, 800 µl49967392 1 49967392 FEP tubing 1000 x 2.5 x 1.5 mm, 1600 µl4701177592 1 4701177592 Rinsing station drain tubing, 1000 x 6.5 x 4 mm

Mechanical54081252 3 54081252 Adhesive retainer for transfer tubing1704731 1 15074002 Polypropylene tray (213 x 91.5 mm for 221) (393 x 244 mm for 222)

For the 183 mm stroke arm36078143 1 36078143 GSIOC cable for 22X to model 401, 402 or Minipuls connection360831221 1 360831221 RS232 cable for connecting sampler to PC (only for XL with 735)52903071 1 5290371 Self-adhesive label for GSIOC identification638306512 4 638306512 6-pin barrier strip connectors for electrical control socket2749592 1 2749892 Liquid level detector cable (400 mm for 221, 480 mm for 222)

DocumentationLT801205 1 LT801205 User’s Guide

Page 67: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-13

Parts & Accessories Lists Appendix A

Accessories

Arms and needle related items

With 221 and 222XL Generics, there is the possibilityof replacing the standard 123 mm stroke verticalarm with either a 56 mm or 183 mm stroke arm.

The 56 mm stroke arm is used for racks in the upperposition containing vials with septa.

The 183 mm stroke arm is for use with longer tubes.

Below is a list of the various accessories which canbe used in conjunction with each of the three arms:

Model 221XL Model 222XLPart Number Part Number Description

For the 56 mm stroke arm270610 270610 56 mm stroke vertical arm27067271 27067271 Bevelled edge constricting needle, for use with TransferPorts,

316L, 93.5 x 1.5 x 1.1 mm27077113 27077113 Constricting needle, 316L, 93.5 x 1.5 x 1.1 mm

For the 123 mm stroke arm27062634 27062634 Needle holder, polyamide (for bevelled edge needle)27077213 27077213 Constricting needle 316L, 160.5 x 1.5 x 1.1 mm27067272 27067272 Bevelled edge constricting needle, for use with TransferPorts,

316L, 160.5 x 1.5 x 1.1 mm27067212 27067212 Side aperture needle for septum piercing and/or liquid mixing,

316L, 160.5 x 1.5 x 1.1 mm27067276 27067276 Flat tipped needle for drop transfer, 316L, 165 x 0.7 x 0.4 mm27067277 27067277 Needle sleeve for 27067276, 155 mm, flat tipped without “V”-cut27367513 27367513 Teflon needle kit for applications where contact with metal is

undesirable (contains: Teflon needle, 157.5 x 0.9 i.d. ; Special needleholder ; Needle guide)

27167215 27167215 Double needle kit (contains: Flat tipped needle, 181.5 x 1.5 x 1.1 mm ;Triangular flat tipped needle, 169.5 x 2.5 x 1.1 mm ; PVDF connector ;Special needle holder ; Needle guide)

For the 183 mm stroke arm270630 183 mm stroke vertical arm27067361 Bevelled edge needle, 316L, 220.5 x 1.5 x 1.1 mm27067373 Constricting needle, 316L, 220.5 x 1.5 x 1.1 mm27067374 Bevelled edge constricting needle, for use with TransferPorts™,

316L, 220.5 x 1.5 x 1.1 mm27067362 Side aperture needle, for septum piercing and/or liquid mixing

316L, 220.5 x 1.5 x 1.1 mm27367423 Teflon needle for applications where contact with metal is

undesirable (contains: Teflon needle, 220.5 x 0.9 mm i.d. ; Specialneedle holder ; Needle guide)

2749870 222 Heightening kit (contains: 4 heightening legs, 65 mm ; 2 tray railsupport bars)

Parts L

ists for 221 an

d 222X

L G

enerics

Page 68: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix A-14

Parts & Accessories Lists Appendix AP

arts

Lis

ts f

or

221

and

222

XL

Gen

eric

s Other Accessories

Model 221XL Model 222XLPart Number Part Number Description

270230 270230 Sampler controller keypad with RS 232 coiled cable21067720 21067720 Gilson 720 sampler keypad software package2106773523 2106773523 735 Sampler Software

2749767 2749767 Injection port2949192 2954706 TransferPort holding bar (221: 8 tubes, 222: 16 holes)2954709 2954709 TransferPort accessory kit (contains: Polyamide transfer port

2800 µl, FEP tubing, 1000 x 3 x 2 mm, fitted with filter 316L, 40 µm ;Sealing caps)

130742 2707404 Holding bar for racks series 0 to 102749872 Metallic front tray assembly (contains: Tray platform and arms

reference 290410 ; 4 heightening legs, 65 mm)

Page 69: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-1

GSIOC Appendix B

GSIOC stands for Gilson Serial Input Output Channel. This communications channellinks all of the Gilson modules in a system together. The system controller (master)controls all of the modules in a system by sending GSIOC commands to the slave modules.Pumps and detectors are considered as slave modules in a Gilson system. Each deviceconnected to the GSIOC channel is distinguished by a GSIOC identification (ID) numberbetween 0 and 63. The GSIOC identification number is set by switches on each moduleor by the module software for certain devices (for example, the 305 piston pump IDnumber is set in the configuration menu). The system controller communicates with oneslave device at a time. GSIOC commands can also be used to control Gilson modulesdirectly from a PC.

Instrument ID Number

All samplers* 10All high pressure pumps** 1401 Dilutors/402 Syringe Pumps 0Minipuls 3 30

* All samplers = 221, 222, 231, 232, 233, ASPEC,ASPEC XLi.

** Applies to 305, 306, 307, 308 Piston Pumps.

The following are the default values (factorysettings) for samplers and pumps:

If you have more than one instrument of thesame type, each instrument must have aunique ID number.

To change the ID number on an instrument,refer to its User’s Guide.

There are two types of commands: buffered commands and immediate commands.

Buffered commands are executed as background processes. They are used to sendcommands.

Immediate commands have a higher priority; the execution of a buffered command willbe interrupted so that an immediate command can be executed. They are mainly used toreturn messages from slave devices.

Page 70: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-2

GSIOC Appendix BIm

med

iate

Co

mm

and

s Immediate Commands

Command Description

% Return the configuration of the sampler

Response format: mmmBVn.nn wheremmm is 221, 222, 231, 232 or 233B indicates that the instrument is a version XL samplerVn.nn is the sampler version number

The identify has been loaded at final inspection in the non-volatilememory using the buffered % command

$ Master reset

Response format: $ whereThe returned $ indicates that the machine has been reset in its power-up state.

? Mode status request

Response format: P whereP indicates that the instrument is operating in primitive mode.

For compatibility with version A samplers only.

B Returns the contents of the command FIFO from the next commandto execute to the end of the FIFO (the maximum capacity of the FIFO being900 characters.

Response format: The unexecuted contents of FIFO whereThe FIFO can contain up to 900 characters, it is sents in segments. Eachimmediate B command returns a segment. Each segment corresponds to abuffered command previously sent to the sampler. As the commands are readback, they are removed from the FIFO.

F Flag computer -> samplerdetermines if the sampler is waiting for a GSIOC command to another module or not.

Response format: uutc whereuu is the unit ID of the slave being interrogated.t is the type of command expected, B for buffered, I for immediatec is the letter of the command.

Example: 05IDThe sampler waits for an immediate command I sent to ID = 5.If the sampler is not waiting, the string returned is ‘-’ (the minus sign).

G Restarts the execution of the command queueand returns G (if the command queue has already been paused by theimmediate H command)

Response format: G

H Stops the execution of the command queue

Response format: H

Page 71: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-3

GSIOC Appendix BIm

med

iate Co

mm

and

s

Command Description

I Read contact inputs

Response format: ccccc whereThe first four parameters represent the four logic inputs, possible values 1or 0 for each one. If the 5th input is switched to analog, 0 represents apositive input.

Example: 10010

J Returns the state of the conatact outputs

Response format: cccccccc whereThe status of each output (1 to 8 respectively) is given. The first threeoutputs are relay outputs, the next four are open collector outputs, and thelast is the front panel low pressure valve connector (0 = contact inactive,1 = contact active). Each c is 0 or 1.

Example: 11101001

N Returns the state and current sensitivity of the liquid level detector

Response format: Innn whereI represents the position of the needle (A for needle in air, L for needlein liquid)nnn is the current sensitivity setting, ranging from 0 to 255. The greaterthe number, the lower the sensitivity.

Example: A10

S Returns the sampler’s state

Response format: ab wherea is 0 if the command queue is empty, otherwise a = 1.b is 0 if the queue is executed normally, 1 if paused or in error.An error can be checked by using the immediate commands x, y, z and P.A returned string of ‘00’ indicates the sampler’s at rest.

V Read the absolute value of the analog input

Response format: decimal number in range 0 to 127 whereThe value (0 - 127) represents the percentage of the full scale. Softwareselectable as full scale value of 10 mV or 100 mV. Any voltage exceeding theselected range is returned as 127.

This command is for compatibility with version A samplers only. See ‘v’.

X Reads the X and Y coordinatesrelative to the home position in units of 0.1 mm.

Response format: xxxx/yyyy whereThe number of digits may vary according to the value. The letter R aloneis returned if the motors are running. If the position is negative, thecorresponding position returns ‘0’.

The home position is the position where the arm goes at power on. This position is set inregisters 33 and 34, and the value of these registers is subtracted from the coordinates in thesampler’s work space as returned by the immediate commands x and y. If the value in any ofthese registers is 65535, it is not subtracted and the value returned by the immediate x commandand that returned by one or both of the immediate commands x and y are equal.In the small size XL sampler, the X-coordinate of any object in the work space is shifted to theleft by 5 mm relative to the version A sampler.

This command is for compatibility with version A samplers only, see ‘x’ and ‘y’.

Page 72: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-4

GSIOC Appendix BIm

med

iate

Co

mm

and

s

Command Description

Z Reads Z position relative to top position

Response format: zzzz wherezzzz is the distance from the top position in units of 0.1 mm. The numberof digits may vary according to the value. The letter R alone indicates thatthe motor is running. If the position is negative with respect to the ‘home’position, ‘0’ is returned.

This command is for compatibility with version A samplers only, see ‘z’.

@ Reads the value from one of the cells of non-volatile memoryThese values include the home and full-scale positions for the three axes,the analog input and various configuration parameters.

Response format: The value returned is a decimal number whereThe address from which the value is taken is set by the buffered @ command.

Example: 203

f Sets a flag in the samplerso that after completion of the current buffered command, all other commands inthe FIFO are ignored and the contents of the FIFO are flushed.

Response format: fIf the current buffered command is F (freeze the interpretation of the buffer), thisforces the termination of the waiting as if the event had occurred. The immediatecommand S will return ‘00’.

n Returns the frequency of the liquid level detector oscillator

Response format: Frequency value in Hertz.

v Returns the voltage at the analog input

Response format: sddddd:sddddd:sddddd whereddddd is a decimal number in the range -1000 to +1000 representing thevoltage range -100 mV to +100 mV.s is the appropriate sign + or -.The three values are: the instantaneous value, the lowest value, and thegreatest value since the last reading.

Example: +00289:-00026:+00987

x, y or z Returns the status of the relevant axis according to the format

Response format: Iddddd whereI can have the following values:

U, the axis is unpowered (free)P, the axis is powered and steady,R, the axis is moving,E, the axis is in error (in this case it is unpowered).

ddddd is the actual position in 0.1 mm of the axis relevant to the ‘home’position. If the position is negative relative to the home position, the format isI-ddddd.

The error condition flag is reset each time a buffered command acts upon thecorresponding axis.The coordinates are expressed in the sampler’s work space. Do not confuse withthe coordinates returned by the immediate X command that are compatible withthe convention used for version A.

Page 73: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-5

GSIOC Appendix BB

uffered

Co

mm

and

s

Buffered Commands

Command Description

F Freeze the interpretation of the buffer and interrogate the GSIOC commandsfollowing along the bus until the specified command is sent to another slave.Then resume the interpretation of the buffer and execute the next movement.The typical use is to start a vertical motion when the dilutor is started, so as tofollow the displacement of the surface of the liquid.

Syntax: Fuutc whereuu is the unit ID of the slave to be interrogated.t is the type of command, B for buffered, I for immediate.c is the letter of the command.

Example F015D

I Switches one of the valves according to the format

Syntax: In for the injection valve - this is not valid for 221 or 222, In/ for the switching valve - use this syntax for 221 or 222, where

n is 0 to turn the valve into the Load position, and 1 to turn to theInject position.

In/ is the same as above but operates the switching valve.This command does not proceed until any current the valve switching iscomplete. If an error is detected, the pause flag is set and execution of thequeue is stopped. Only one valve can be switched at a time.

J Sets the outputs and the low pressure valve

Syntax: Jabcdefgh wherea is 0 to energize the first relay, 1 to release it, or X to keep its current stateb and c have the same effect on the second and third relays respectively.d is for the first open collector output.e, f and g are the second, third and fourth open collector outputsrespectively.

Example: J00110001This command cannot be truncated. If only the first relay must be operated, thecommand J1XXXXXXX must be sent.

N Sets the sensitivity of the liquide detector

Syntax: Nnnn wherennn is a value from 0 to 255.

The smallest numbers give the highest sensitivity. However an excessivesensitivity could produce undesired detections. If no value is supplied, thesensitivity is unchanged. In both cases, the state of the detector is reset to ‘A’ (air).This command must be followed by the sequence @* to make the change permanent.Default value: 10

T Wait until the specified time has elapsed.

Syntax: Tttttt wherettttt is the delay ranging from 0 to 6000 in 1/100 ths of a second (6000 = 1 min).

If the value exceeds this limit, the command is ignored. The command with noargument is the equivalent to the execution of the immediate H command: thesampler waits until the immediate G command is sent.

Page 74: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-6

GSIOC Appendix BB

uff

ered

Co

mm

and

s

Command Description

V Sets the sensitivity of the analog input when interrogatedwith the immediate V command.

Syntax: Vd whered is either 0 or 1, setting the full-scale respectively to 10 mV or 100 mV.

The default value is set at delivery to 10 mV. The command must be followedby the sequence @* to make this change permanent.This command is for compatibility with version A samplers only.

W Stops the execution of the queue until all three axes have reachedtheir destinations, or are stopped because an error has been detected.In the case of an error being detected, the execution is paused and the pauseflag is set. The flag can be read back using the immediate S command.

X Sends the arm to a pair of coordinates in the XY plane relative the‘home’ position

Syntax: Xxxxx/yyyy wherexxxx is the x-coordinate in 0.1 mmyyyy is the y-coordinate in 0.1 mm

0/0 is the home position.The home position is the position where the arm goes at power on.This position is set in registers 33 and 34, and the value of these registers is addedto the parameters supplied for the current command. If the value of any of theseregisters is 65535, it is not added and the value to supply to the command X andthat supplied to either or both of the immediate commands x and y are equal.In the small size XL sampler, the x-coordinate of any object in the work space isshifted left by 5 mm relative to the version A sampler.This command is for compatibility with version A samplers only.

Z Sends the arm to the specified height

Syntax: Zzzzz is the coordinate in 0.1 mm, 0 is the top position.This command is for compatibility with version A samplers only, see ‘A’.

@ Writes a value into one of the cells in the buffer of the non-volatile memory.These values include the home and full scale positions for the three axes,the analog input and various configuration parameters

Syntax: @aa or @aa=vvvvFor the command @aa,

aa is the decimal address of the cell: the command sets the pointer to thiscell for subsequent reading. THe address ranges from 0 to 54.

For the command @aa=vvvv,aa is the address of the cell,vvvv is the value assigned to it.

Each value ranges from 0 to 65535 except for the first five values that rangefrom 0 to 99 000 000. The values are changed in the EEPROM buffer only, thechange is temporary. The previous values will come back when the sampler isreset with the immediate $ command or the next time the sampler is switched on.If it is desired that the change is permanent, the command must be followed bya ‘*’ that saves all the configuration parameters into the EEPROM at once.Examples: @12* selects the cell 12 and saves the previous changes.@12=458* writes the value 458 into the cell # 12 and saves the previous changes.@* only, saves all the previous changes.The function of the 34 pre-assigned memory cells is given in the table in the 719User’s Guide, Appendix C.

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Appendix B-7

GSIOC Appendix BB

uffered

Co

mm

and

s

Command Description

v Sets the speedalong the specified axis (X, Y or Z)

Syntax: vaxxxx wherea is the axis name (X, Y or Z), capital letter.xxxx is the speed in 0.1 mm/sec.

If the buffered command @* is sent before the sampler is switched off, thisspeed becomes the default value at the next power on.Value ranges: X and Y = from 1000 to 2500, Z = from 1 to 1250Default values: X and Y = 2500? Z = 1250

z Allows individual axis control

Syntax: zcnnnn wherec is the axis letter, X, Y or Znnnn is the absolute coordinate in 0.1 mm: 0 is the home position for X andY, and the top position for Z.

If the value is preceded by a + or a - sign, the coordinate is considered relative tothe current position, that is z+10 means ‘lower the needle by 1 mm’.A variant clnnnn where l is the lowercase ‘L’ letter and allows a travel to bestopped if the liquid level detector senses the liquid before the specified positionhas been reached. The actual position can then be read back by sending thecorresponding immediate command (x, y or z).If the coordinate is replaced by a star (*), the corresponding axis is unpowered(the motor is free). Sending a command with no coordinate at all (X, Y or Zalone) reconnects the motor without moving it. It is also reconnected by any ofthe motion commands that affect the corresponding axis.

Page 76: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix B-8

GSIOC Appendix B

Page 77: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-1

Technical Data Appendix C

This chapter contains the Technical Data concerning the 221/222 Liquid Handlers.

Application: Sample preparation (dilution, serial dilution, addition of reagents andsolvents, any liquid transfer from tube to tube, mixing), analyzer loader.

Page 78: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-2

Technical Data Appendix CH

ydra

ulic

s Hydraulics

Vial Capacity

From 120 microvials of 0.3 ml for the 221XL or 540microvials of 0.7 ml for the 222XL up to 4 bottles of250 ml.

Choice of 25 standard Gilson racks, including 5thermostated racks, plus special racks, a bar that canhold up to 8 TransferPorts for the 221XL or 15TransferPorts for the 222XL, and free vesselprograming.

Syringe Capacity (402 Syringe Pump)

0.1, 0.25, 0.5, 1, 5, 10 and 25 ml.

Liquid Flow Rate (402 Syringe Pump)

From 0.01 to 120 ml/min., continuously.

Liquid-contact Materials

316L stainless steel or titanium, FEP, PTFE, PEEK,and PP.

Needle Gas-supply

Through 3-port valve (additional accessory) forevaporating, degassing and mixing.

Sample Cross-contamination

Lower than 1 ppm, with liquid level detector andrecommended values for rinsing parameters (4 mmimmersion, 20 ml/min., 1 ml and 2 ml for needleinside and outside respectively), and lower than 50ppm without liquid level detection.

Page 79: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-3

Technical Data Appendix CM

echan

ics

Mechanics

Arm Motion Speed

250 mm/s horizontal, 125 mm/s upwards and 90mm/s downwards.

Needle Tip Positioning

± 0.2 mm reproducibility and ± 0.75 mm accuracy ineach direction.

Page 80: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-4

Technical Data Appendix CE

lect

rica

l Electrical

Remote control

RS-232C and GSIOC.

Inputs

4 electrical contacts and one analog signal (100 mV),convertible as a 5th contact.

Outputs

7 electrical contacts including 3 relays, plus twopower supplies for 3-port valve and other auxiliaryequipment (12 VDC, 500 mA).

Page 81: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-5

Technical Data Appendix CC

on

trol

Control

Gilson Sampler Controller Keypad viaRS-232C

8-line, 40-character display, 24 keys, with Gilson 720Keypad Software (3.5", double-sided, high-density,1.44 MB diskette).

Microcomputer via RS-232C or viaGSIOC through Gilson 506 Interface

Gilson 719 PC Software: DOS 3.1 or higher,Microsoft Windows 3.1 or higher, 4 MB RAM.

Gilson 735 Sampler Software: 120 MHz Pentium®

Processor, 32 MB RAM, Windows® 95 or NT® 4.0, 50MB hard disk space.

Page 82: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-6

Technical Data Appendix CP

rog

ram

s Programs

Automated Laboratory Tasks Available

Add, aliquot, calibrate, dilute, dilute and add, diluteserially, dispense, mix, move arm, pipette, rinseneedle, set electrical contact and wait.

Size

Unlimited (more than 1MB available on 720diskette).

Storage

Permanent (for more than 100 programs on 720diskette).

Page 83: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-7

Technical Data Appendix CE

nviro

nm

ental C

on

ditio

ns

Environmental Conditions

Storage

Indoor use only.

Installation: Category II.

Altitude: Up to 2000 m.

Temperature range: 5°C to 40°C.

Pollution degree 2.

Humidity: Maximum relative humidity 80% fortemperatures up to 31°C decreasing linearly to 50%relative humidity at 40°C.

Power Requirements

Frequency: 50 to 60 HZ.

Voltage: 100 to 240 V ac, mains supply voltagefluctuations should not exceed ±10% of the nominalvoltage.

Power rating: 110 VA.

Each XL instruments is subjected to a Dielectric Test(1500 V between live/neutral and earth) and anEarth Bonding Impedance Test (� 0.1 �).

Size and Weight

221XL: (W x D x H) 330 x 465 x 230 mm, 12.2 kg.

222XL: (W x D x H) 515 x 620 x 230 mm, 16.2 kg.

Page 84: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix C-8

Technical Data Appendix C

Page 85: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix D-1

Reference Information Appendix D

This chapter contains two tables, one indicates which liquids are acceptable for use withdifferent materials. The second table indicates which racks are compatible with thedifferent sampler models.

Page 86: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix D-2

Reference Information Appendix DC

hem

ical

Iner

tnes

s o

f P

last

ics

Use

d Chemical Inertness of Plastics Used

Plastics used are:

- Polytetrafluoroethylene (FEP),- Polypropylene (PP),- Polyvinylidene fluoride (PVDF).

FEP has a high chemical inertness to commonliquids. PP and PVDF have a high chemical inertnessto aqueous, acid and basic solutions, at ambienttemperature:

where: + for acceptable0 for exposure- for unacceptable

Organic solvent b.p. in °C PP PVDF

Acetic acid (glacial) 118 + +Acetone 56 + -Acentronitrile 82 + +Benzene 80 + +Carbon tetrachloride 78 - +Chloroform 61 - +Cyclohexane 81 - +Dioxane 101 + 0Diethylether 35 + +Ethyl acetate 77 + +Heptane 98 0 +Hexane 69 + +Ketones (general) + +Methanol 65 + +Methylene chloride 40 0 +Tetrahydrofuran (THF) 66 0 0Toluene 111 0 +Trichloroethylene 87 0 +

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Appendix D-3

Reference Information Appendix D

Rack Compatibility Table

Rack C

om

patib

ility Table

Bot

tleA

lum

inum

Pol

ypro

pyle

neT

ube

Rac

ks

Free

123

mm

Z-a

rm a

nd ra

ck h

oldi

ng b

ar(2

7044

71M

)

Fitt

ed w

ith s

mal

l sup

port

arm

s(1

3074

2) a

nd 1

23 m

m Z

-arm

Sta

ndar

d co

nfig

urat

ion:

Fitt

ed w

ith P

olyp

ropy

lene

rack

tray

and

123

mm

Z-a

rm

Sta

ndar

d co

nfig

urat

ion:

Pol

ypro

pyle

ne tr

ay a

t wor

ktop

leve

lan

d 12

3 m

m Z

-arm

Fitt

ed w

ith ra

ck h

oldi

ng b

ar fo

r one

rack

(270

7404

)

Fitt

ed w

ith m

etal

lic h

oldi

ng b

ar(2

7044

73)

Fitt

ed w

ith h

eigh

teni

ng k

it (2

7498

72),

plat

form

in H

IGH

pos

ition

, pol

ypro

pyle

netr

ay a

nd 1

23 m

m Z

-arm

Fitt

ed w

ith h

eigh

teni

ng k

it (2

7498

72),

plat

form

in L

OW

pos

ition

, pol

ypro

pyle

netr

ay a

nd 1

83 m

m Z

-arm

Fitt

ed w

ith h

eigh

teni

ng k

it (2

7498

70),

poly

prop

ylen

e tr

ay o

n w

orkt

op a

nd18

3 m

m Z

-arm

221XL 222XL

guiding bar13040103

tubes � 100 mm longtubes up to

180 mm longtubes up to

150 mm longtubes �

100 mm long

Racks

0

1

7

8

9

10

20

21

22

22U

23

24

28

29

30

31

33

34

30

31

32

33

34

60

61

62

92

93

94

F

rack heightener2954694

need

s th

erm

osta

ting

cuve

tte

Polypropylene free rackwith rack heightener

aluminum free rackseries 20 free rack

aluminumseries 20

polypropylenealuminum

polypropylenealuminum

Page 88: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

Appendix D-4

Reference Information Appendix D

Page 89: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations
Page 90: 221 and 222 XL Liquid Handlers User’s Guide - ManSciGilson sampler controller keypad through a RS232 connector. 221 and 222 Liquid Handlers help automate liquid handling operations

World Wide Web: www.gilson.comE-mail: [email protected], [email protected], [email protected]

World HeadquartersGilson, Inc.3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027, USATelephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451

Gilson SAS19 avenue des Entrepreneurs, B.P. 14595400 Villiers-le-Bel, FranceTelephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-20