14

:NaKNaKATP

:

:

FUNCTIONAL MULTINEURON CALCIUM IMAGING (fMCI)

Rat hippocampal slices or slice cultures were loaded with Oregon green 488 BAPTA-1AM, and calcium signals were optically recorded from the CA3 pyramidal cell layer in aCSF consisting of (in mM): 127 NaCl, 26 NaHCO3, 3.3 KCl, 1.24 KH2PO4, 1.0 MgSO4, 1.0 CaCl2 and 10 glucose. Fluorophores were excited at 488 nm and visualized with a 507-nm long-pass emission filter. Images were captured at 10-2000 frames/s with a Nipkow spinning-disk confocal microscope (CSU10, CSU22, and CSU-X1; Yokogawa Electric, Tokyo, Japan), a cooled CCD camera (iXon DU860, DU897, and DV887; Andor, Belfast, Northern Ireland, UK), an upright microscope (ECLIPSE FN1, Nikon, Tokyo, Japan), a water-immersion objective (16X, 0.80 NA, CFI75LWD16xW, Nikon), and Metamorph software (Molecular Devices, Union City, CA).

HP

DNA25000

Nature or NurtureNatureNurture

Dose-dependent effects of prenatal ethanol exposure on synaptic plasticity and learning in mature offspringNature2002

4LH

10

D4

D4 10 20 30 40 50 60 1 MGNRSTADAD GLLAGRGPAA GASAGASAGL AGQGAAALVG GVLLIGAVLA GNSLVCVSVA 61 TERALQTPTN SFIVSLAAAD LLLALLVLPL FVYSEVQGGA WLLSPRLCDA LMAMDVMLCT 121 ASIFNLCAIS VDRFVAVAVP LRYNRQGGSR RQLLLIGATW LLSAAVAAPV LCGLNDVRGR 181 DPAVCRLEDR DYVVYSSVCS FFLPCPLMLL LYWATFRGLQ RWEVARRAKL HGRAPRRPSG 241 PGPPSPTPPA PRLPQDPCGP DCAPPAPGLP RGPCGPDCAP AAPGLPPDPC GPDCAPPAPG 301 LPQDPCGPDC APPAPGLPRG PCGPDCAPPA PGLPQDPCGP DCAPPAPGLP PDPCGSNCAP 361 PDAVRAAALP PQTPPQTRRR RRAKITGRER KAMRVLPVVV GAFLLCWTPF FVVHITQALC 421 PACSVPPRLV SAVTWLGYVN SALNPVIYTV FNAEFRNVFR KALRACC7

D4

D4PAPRLPQDPCGPDCAPPAPGLPRGPCGPDCAPAAPGLPPDPCGPDCAPPAPGLPQDPCGPDCAPPAPGLPRGPCGPDCAPPAPGLPQDPCGPDCAPPAPGLPPDPCGSNCAP 11p15.5

D411p15.5

10 20 30 40 50 60 1 METTPLNSQK QLSACEDGED CQENGVLQKV VPTPGDKVES GQISNGYSAV PSPGAGDDTR 61 HSIPATTTTL VAELHQGERE TWGKKVDFLL SVIGYAVDLG NVWRFPYICY QNGGGAFLLP 121 YTIMAIFGGI PLFYMELALG QYHRNGCISI WRKICPIFKG IGYAICIIAF YIASYYNTIM 181 AWALYYLISS FTDQLPWTSC KNSWNTGNCT NYFSEDNITW TLHSTSPAEE FYTRHVLQIH 241 RSKGLQDLGG ISWQLALCIM LIFTVIYFSI WKGVKTSGKV VWVTATFPYI ILSVLLVRGA 301 TLPGAWRGVL FYLKPNWQKL LETGVWIDAA AQIFFSLGPG FGVLLAFASY NKFNNNCYQD 361 ALVTSVVNCM TSFVSGFVIF TVLGYMAEMR NEDVSEVAKD AGPSLLFITY AEAIANMPAS 421 TFFAIIFFLM LITLGLDSTF AGLEGVITAV LDEFPHVWAK RRERFVLAVV ITCFFGSLVT 481 LTFGGAYVVK LLEEYATGPA VLTVALIEAV AVSWFYGITQ FCRDVKEMLG FSPGWFWRIC 541 WVAISPLFLL FIICSFLMSP PQLRLFQYNY PYWSIILGYC IGTSSFICIP TYIAYRLIIT 601 PGTFKERIIK SITPETPTEI PCGDIRLNAV

17q11.1-q12

17q11.1-q12

**