EFEK KOMBINASI TOMAT RANTI (Lycopersicum pimpinellifolium Mill.) DAN CABE RAWIT (Capsicum frutescens) TERHADAP KADAR Interleukin 1 Beta (IL-1β) SERUM TIKUS WISTAR

YANG DIINDUKSI TAWAS

Novita Septi Fauziah*, Doti Wahyuningsih**, Helmin Elyani***Mahasiswi Pendidikan Dokter Universitas Islam Malang

** Staf Pengajar Pendidikan Dokter Universitas Islam Malangnobytatugas@yahoo.com

ABSTRAK

Pendahuluan: Tawas (Al2(SO4)3) akan dimetabolisme di hepar dan meningkatkan radikal bebas. Kondisi ini akan meningkatakan resiko terjadinya nekrosis sel akibat stress oksidatif. Nekrosis sel akan mengaktifkan makrofag untuk memproduksi sitokin proinflamasi salah satunya IL-1β. Tomat ranti (Lycopersicum pimpinellifolium Mill.) dan cabai rawit (Capsicum frutescens) dapat berpotensi sebagai antioksidan dan anti inflamasi. Berdasarkan data diatas, peneliti ingin mengetahui efek kombinasi tomat ranti dan cabai rawit terhadap kadar IL-1β serum tikus wistar yang diinduksi tawas.

Metode: Disain penelitian control group post test only dengan menggunakan tikus wistar jantan (n=36 ekor tikus dibagi dalam 6 kelompok). Kelompok KN (diberi aquadest), KP (induksi tawas 8% + aquadest), K1 (induksi tawas 8% + tomat ranti50%), K2 (induksi tawas 8% + tomat ranti50% + cabai rawit 12,5%), K3 (induksi tawas 8% + tomat ranti 50% + cabai rawit 25%), K4(induksi tawas 8% + tomat ranti 50% + cabai rawit 50%), semua paparan diberikan secara personde lambung. Kadar IL-1β diukur secara ELISA. Data dianalisis dengan uji one way ANOVA yang dilanjutkan dengan uji LSD (Least Significant Difference). Bermakna apabila p<0,05.

Hasil: Kadar normal IL-1β serum sebesar 43,51 pg/mL. Paparan tawas meningkatkan kadar IL-1β serum sebesar 38% (p=0,00). Paparan tomat ranti 50% menurunkan kadar IL-1β. Pemberian tomat ranti 50% + cabai rawit 12,5 % dapat menurunkan kadar IL-1β sebesar 39%(p= 0,00). Pemberian tomat ranti 50% + cabai rawit 25 % dapat menurunkan kadar IL-1β sebesar 37%(p= 0,038). Dan Pemberian tomat ranti 50% + cabai rawit 50 % dapat menurunkan kadar IL-1β sebesar 0,9%(p= 0,129).

Kesimpulan: Induksi tawas pada tikus meningkatkan IL-1β serum tikus. Pemberian tomat ranti saja mampu menurunkan kadar IL-1β serum. Kombinasi tomat ranti 50% dan cabai rawit mampu menurunkan kadar IL-1β serum.

Kata Kunci: Tawas, Tomat ranti (Lycopersicum pimpinellifolium Mill.), Cabai rawit (Capsicum frutescens), IL-1β serum.

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EFFECT OF COMBINATION RANTI TOMATO (Lycopersicum pimpinellifolium Mill.) AND CAYENNE PEPPER (Capsicum frutescens) ON SERUM INTERLEUKIN 1-1β (IL-1β) LEVEL OF

ALUM INDUCED WISTAR RAT

Fauziah, Novita S*, Wahyuningsih,Doti**, Elyani, Helmin ***Undergraduate programme, Medical Faculty of Malang Islamic University

** Lecturer, Medical Faculty of Malang Islamic Universitynobytatugas@yahoo.com

ABSTRACT

Introduction: Alum (Al2(SO4)3) was metabolized in the liver and produced large amount of free radicals. Which it could increase the number of necrotic cell due to oxsidative stess. The necrotic cell’s activate macrophages to produce pro–inflammatory cytokines,observed is IL-1β. Ranti tomato (Lycopersicum pimpinellifolium Mill). and cayenne pepper (Capsicum frutescens) have an antioxidant and anti-inflammatory potentcy. Based on this data, researchers would like to know the effect of combination ranti tomato and cayenne pepper on serum IL-1β level of alum (Al2(SO4)3) induced wistar rat.

Methods: The design of the experimental was control group post test only design, in male wistar rat (n=36, there are 6 group of rats). Group KN (given only aquadest), KP (alum8% induction+aquadest), K1(alum8% induction + ranti tomato50%), K2 (alum8% induction + ranti tomato50%, + cayenne pepper 12,5%), K3 (alum8% induction + ranti tomato50%, + cayenne pepper 25%), K4 (alum8% induction + ranti tomato50%, + cayenne pepper 50%), all of the exposure is given by naso gastric sonde. IL-1β level were measured by ELISA. Data were analized by one-way ANOVA test followed by LSD (Least Significant Difference) test. Significant when p<0,05.

Result: Serum IL-1β level of KN group was 43,51 pg/mL. Induction of alum incrased serum IL-1β level as much as 38% (p=0,00). Group KP of 50% ranti tomatoes decreased serum IL-1β. Combination of 50% ranti tomato + cayenne pepper 12,5% decreased serum IL-1β level as much as 39%(p= 0,00). Combination of 50% ranti tomato + cayenne pepper 25% decreased serum IL-1β level as much as 37%(p= 0,038). And Combination of 50% ranti tomato + cayenne pepper 50% decreased serum IL-1β level as much as 0,9%(p= 0,129).

Conclusion: alum induction to rats increased serum IL-1β level. Supplementation of ranti tomato 50% decrease serum IL-1β level. Combination 50% ranti tomato with cayenne pepper dereased serum IL-1β level in alum induced rats.

Keyword: Alum (Aluminium sulfate), Ranti tomato (Lycopersicum pimpinellifolium Mill), Cayenne pepper (Capsicum frutescens), serum IL-1β .