Acta Medica OkayamaVolume 3, Issue 3 1932 Article 5

MARZ 1933

On the Isolation of Antibodies fromimmunized Serum-globulin.

Takeiti Asaba∗

∗Okayama University,

Copyright c©1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.

On the Isolation of Antibodies fromimmunized Serum-globulin.∗

Takeiti Asaba

Abstract

1. It is possible to isolate the immune bodies from the globulin fraction, which was obtainedby the salting out with ammonium sulphate or electrodialysis, by the biologic method. The rateof isolation is almost the same as the rate of the isolation by the biologic method. 2. The isolatedserum from the globulin fractions by the combination of the physical or the chemical with thebiologic method, has less antigenic and nitrogen contents than the isolated serum by the biologicmethod alone; especialy the isolated immune substance by the combination of the physical with thebiologic method has the least antigenic contents. 3. The best results are obtained in the physiologicsalt solution at 65℃, for the isolation of precipitin by means of the combination of thephysical with the biologic method.It is a pleasure to express my indebtedness to Prof. Ogata forthe encouragement and valuable suggestions which he has given. I am also indebted to Dr. Sunoutifor various assistance he has offered in the preparation of this work.

∗Copyright (C) OKAYAMA UNIVERSITY MEDICAL SCHOOL

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Asaba: On the Isolation of Antibodies from immunized

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Asaba: On the Isolation of Antibodies from immunized

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5

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Asaba: On the Isolation of Antibodies from immunized

Produced by The Berkeley Electronic Press, 1932

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Acta Medica Okayama, Vol. 3 [1932], Iss. 3, Art. 5

http://escholarship.lib.okayama-u.ac.jp/amo/vol3/iss3/5