Andrew Pham Duckweed FD

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    Andrew Pham

    Chenchen Shen

    BI213-072

    3/1/12

    The Effect of Fertilizers on Duckweed

    Duckweed also known asLemnoideae, they are tiny water plants that grow in

    mainly pond water. They are high protein plant that grows very rapidly and is used in

    many types of research. My group is interested in what are the effects of All-Purpose

    Fertilizer and Complete Organic Fertilizer on duckweed. From research, I found out that

    increasing levels of N fertilizer in the pond increased biomass yield and crude protein

    content of the duckweed. (Lampheuy, Preston, Thy 2004) With this resource, I can

    hypothesize that with the help of fertilizers, duckweed will be able to flourish. With that,

    knowing only so little about duckweed and its potential, will duckweed actually take

    advantage of the fertilizer in our experiment?

    Our method was to obtain an organic fertilizer, and all-purpose fertilizers. With it

    we diluted 5ml of each fertilizer into 200ml of DI water. We obtained 180 pieces of

    duckweed and placed 20 into 9 petri dishes. 3 was labeled control and 40 ml of water

    was placed in. 3 more petri dished was labeled organic fertilizers and placed 40ml of the

    diluted solution into the dish. The last 3 was done accordingly so. We have a trial of 3

    weeks and the results were recorded each week by counting the duckweed each week.

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    Based on the data we

    recorded, we had some

    interesting results. The best

    treatment on our experiments

    was the control group. Based on

    Figure 1 the population of the

    control group flourished over

    time. The worst treatment was

    the complete organic because

    due to the increase of pH its

    population was decreased to

    zero. Figure 2 shows the standard deviations for all the treatment and how the numbers

    were distributed. The significance of the results is that the duckweed was able to grow

    without the presence of fertilizers, and therefore my hypothesis was incorrect because in

    this case the duckweed did not benefit from the fertilizers.

    My results did not support my hypothesis. I believe the reason why our duckweed

    in the organic fertilizer died is because the concentration in the petri dish is high and that

    we over fertilized our duckweed. Our organic fertilizer had a 6-7-7 ratio of nitrogen-

    phosphorous-potassium. Due to the fact that its being over fertilize of phosphorous, more

    phosphorous increase pH levels in the soil and could kill plants (Mitchell and Adams

    2000). Plants wants to be around the 6-7 pH and so conclude that the fertilizers increased

    the pH level in the water, which resulted in reductions of duckweed. These result are

    important because it could help future consumers who would used fertilizers could use

    Figure 1. This chart shows the relationship between the populations

    of the Lemnoideae,duckweed of all three experiments (control,

    complete organic and all purpose fertilizers). The control group

    flourishes where as the complete organic fertilizers killed theduckweed after the first week. As for the all purpose fertilizers, it

    grew after the first week but then died.

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    my results and not make the same mistake when they are using fertilizer and not over

    fertilizing their plants.

    In conclusion, this experiment would have worked if we didnt have to work with

    such small petri dishes therefore my hypothesis was not supported. The presence of

    fertilizers inhibits growth of duckweed based on my experiment. In future experiments, I

    would have used a larger container and used fewer fertilizers to prevent the pH level from

    increasing and killing the duckweed.

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    Figure 2. This graph shows the standard deviation of all the treatments and how the numbers were distributed.

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    Works Citation

    Lampheuy Kaensombath, Thy San and Preston T R 2004: Manure or biodigester

    effluent as fertilizer for duckweed.Livestock Research for Rural Development, Vol. 16,

    Art. #17. Retrieved February 8, 112, from http://www.lrrd.org/lrrd16/3/lam16017.htm

    Mitchell, Charles, and James Adams. "Lowering Soil PH." Clemson University,

    17 Jan. 2000. Web. 27 Feb. 2012. .