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Anne-Sophie MONTCUQUET 1,2, Lionel HERVE 1, Jean-Marc DINTEN 1, Jérôme I. MARS 2
1 LETI / LISA – CEA, Minatec, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France. 2GIPSA-Lab / Dept Images – Signal, 961 Rue de la Houille Blanche, BP 46, 38402 Saint Martin d'Hères, France.
IntroductionFluorescent imaging in diffusive media is an emerging imaging modality for medical applications: injected fluorescent markers (in multiplexing, several specific markers are used) bind specifically to targeted compounds, like carcinoma. The region of interest is illuminated with near infrared light and the emitted back fluorescence is analyzed to localize the fluorescence sources. For medical diagnostic application, thick media have to be investigated: as the fluorescence signal gets exponentially weak with the light travel distance, any disturbing signal - such as biological tissues autofluorescence - may be a limiting factor.
To remove these unwanted contributions, or to separate different fluorescent markers, a spectroscopic approach and a blind source separation method are explored. We present in this poster a feasibility experiment on an optical phantom in which a marked tumor is simulated. We show how an NMF unmixing preprocessing eradicates the autofluorescence signal of the phantom and allows to get more accurate 3-D reconstructions of the specific marker by Fluorescence Diffuse Optical Tomography (FDOT).
Fluorescent imaging
Image CEA
Non-negative Matrix Factorization
Therapeutic window
Formal statement
NMF applied to spectroscopy
Given a non-negative matrix , find non-negative matrices
and such that:
(P stands for the number of fluorescent sources to unmix)
Challenge
We want to unmix several fluorescence spectra:
A spectroscopic approach is chosen.
We do not have much information about the fluorescence spectra :
A blind source separation method is required.
Conclusion
Algorithm
Multiplicative update rules
Update of A:
1. Initialization of matrices A (constant) and S (spectra models) with A0 and S0 > 0
2. Minimization of the cost function F
Update, in turn, of A and S
Algorithm steps
Fluorescent probes location
Non-negative Matrix Factorization: a blind sources separation method applied to optical fluorescence spectroscopy and multiplexing
The use of red light limits the biological tissues absorption
Injected fluorescent markers bind specifically to a given molecule
Experimental set-up
Update of S:
V
The fluorescence signal is collected along a line of Nx detectors by a spectrometer coupled with a CCD camera: a Nx x Nλ acquisition is measured. A translation stage, covering Ny steps, is then used to get a scanning of the whole object.
Feasibility experiment Results (1/2)
Autofluorescence (PPIX)
ICG-LNP
Results (2/2)NMF decomposition gives two distinct fluorescence
spectra
. An original regularized NMF algorithm is used.
. Experiments were performed ex vivo on optical phantoms to assess the capacity of NMF to unmix overlapping specific fluorescence and autofluorescence spectra.
. The NMF algorithm is also suitable for in vivo experiments.
. Spectrally resolved acquisitions combined to NMF processing successfully separate different fluorescent markers or filter different fluorescence contributions of interest from measurements impaired by autofluorescence.. NMF preprocessing improves FDOT reconstructions of specific fluorescent markers distributions by removing the disturbing fluorescence signals
Intensity data
Forward model: finite volume method
*Andor technologies
**
690 nm