Purpose/Objective(s)
A combined X-ray contrast and optical imaging provides
physiological and functional details of
normal or diseased tissue non-invasively at a molecular level.
Through real time visualization,
image acquisitions, delineating the tumor boundaries, the
integration of optical molecular
imaging into the radiation treatment allows effective use of
radiation in radiation oncology. The
design, construction, and validation of Molecular Image Guided
Radiation Therapy (MIGRT)
instrumentation for preclinical theragnostics will be
described.
Materials/Methods
The multimodal molecular imaging module with appropriate
shielding is engineered into
a steel lined x-ray irradiator chamber. The MI module includes a
stage to immobilize and
position small animals (mice to rabbit) or tissue culture
plates. Phosphor screens between the
animal chamber and the detector facilitate both the optical and
radiographic images to be
captured at the same focal plane. A cooled charge-coupled device
(CCD) based system images the specimen in X-ray mode and optical
mode to allow co-registration of luminescent tumors or target
tissues on an X-ray background of the animal or other samples.
RT is achieved using x-ray tube
with a homogenic beam powered by 320 kV stable X-ray generators
using fixed and/or
adjustable collimators.
Oral Presentation By
Dr. Rao Papineni
Development of Integrated Multimodal Molecular Imaging and X-ray
Irradiator Module for Molecular Image Guided Radiation Therapy -
MIGRT in Preclinical Research
Results
The design and fabrication of the integrated MIGRT, its
validation, and the performance are
completed. The radiation treatment protocol (whole body)
followed by functional molecular
imaging of the reactive oxygen species (ROS) response were
performed on swiss albino mice.
Significant changes in the luminescence signals a response to
the changes in ROS activity were
acquired real time without moving the animal or other specimen
between imaging modalities or radiation treatment.
Conclusions
Efficient integration of the X-ray irradiation system capable to
perform both optical and X-ray imaging of their cells, tissues or
animals for pre-treatment MIGRT localization of target tissues
and
for post treatment diagnostics of results at a molecular and
functional level is accomplished.
Functional and molecular imaging during or post RT also opens
novel opportunities to determine
and explore the molecular mechanisms in radiation biology-
thereby developing new therapeutic
strategies complementing radiation therapy- with a distinct
footprint in immunotherapy, adoptive
cell therapy, and targeted chemotherapy.
PI: Dr. Rao V. L. Papineni - [email protected]
Coauthors: William McLaughlin, Brian Dermott, Douglas Vizard,
and Juan C Gonzales. PACT & Health LLC
Precision X-ray Inc
& KUMC USA.
American Society for Radiation Oncology, ASTRO's Annual Meeting
is the premier radiation
oncology scientific event in the world and draws more than
11,000 attendees each year. During
the 2014 Annual Meeting, we will highlight science that
showcases how technology and biology
advance the field and improve patient outcomes and quality of
life.
