BIO111 Μικροβιολογια ∆ιαλεξη 5 Απο γονιδιο στιςecoserver.imbb.forth.gr/microbiology/documents/Lec5.pdf · ΓΙΝΕΙ ΒΑΚΤΗΡΙΑKΗ ΜΕΤΑΦΡΑΣΗ

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  • BIO111 5

  • Yeast

  • ?

  • ( )

    1. (DNA) ()

    2. 4 -

    3. I.II.III.5->3IV.->CV. VI.3 =1

  • -To

    You are here

  • http://www.biologie.uni-hamburg.de/lehre/bza/1ihf/e1ihfm.htm

    DNA

    IHF(E. coli)

    HU(B. stearothermophilus)

    TF1(B. subtilis)

  • http://www.biologie.uni-hamburg.de/lehre/bza/1ihf/e1ihfm.htm

    DNA IHF(Integration Host Factor)

    E. coli

    DNA

  • DNA

    1. 2.

    3. ()

  • -/

  • ...

    ...DNA

  • DNA

  • DNA

  • DNA

  • -

  • -

  • 1 1

    1 RNA

  • =

  • -

    DNA?

  • -, .

    RNA.

  • RNA

    1. 2. O

    3. ()

  • M = 5' -> 3'

  • A K

    1.DNA ()2.RNA (+)3.4.ATP5. 6.

  • ?

    5 3

    T85T83G81A61C68A52

    T89A81T50A65A65T100

  • -

    ?

  • - RNA

  • RNA

    - +

  • -

  • RNA ?

    5 3

  • 70 DNA?

  • E.coli

    CD-ROM

  • +1 G

  • -

  • DNA

    http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/chroms-genes-prots/RNAP-supercoil.html

  • RNA

  • -

    G

    U

    CCC

    UC

    AU

    GGG

    =

    =

    = U U U

    U

    G

    UCA

    UA

    CU

    AGU

    =

    =

    =

    GC

    AU

  • M

  • -

    RNA?

  • -, .

  • M = H2 COOH mRNA

    5' -> 3'

  • (

    )

    ()

    , , C, S, P, H ....

    DNA, RNA

  • A K

    1.mRNA2.tRNA-aa3.30S+50S = 4.GTP, ATP5. 6. 7.

  • -

    -35 -10 +1

    5 3

    5

    5

    3

    3

    RNA

    5 UTR 3 UTR

  • -

    -35 -10 +1

    5 3

    5

    5

    3

    3

    RNA

    5 UTR 3 UTR

    C

  • -

    -35 -10 +1

    5 3

    5

    5

    3

    3

    RNA

    Shine-Dalgarno

  • AUUCCUCCAC

    30S 16S RNAUAGXXX

    OH

    XXXAGG GG GA AU U CXXXXXXAUGXXX...

    5'-

    -3'mRNA

    Ribosome inding Site A

    Shine-Dalgarno : AGGAGGU

    SHINE-DALGARNO

    3'

  • tRNA

  • tRNA

  • tRNA mRNA

  • E

  • -

  • -

  • CD-ROM

  • -

    DNA

  • CD-ROM

  • ..

  • ()

  • ?

  • H -GroEL-ES

    Top view into the cavity of the top ring. The domain rotation caused by nucleotide binding continues into the large movement accompanying GroES binding.

    Bottom view.Different pattern of movement in the lower ring. The upper ring (at the back of the images) performs a continuous twisting motion, but the lower ring opens and shuts with radial movements of the domains in addition to the twisting.

  • -

    ?

  • =

  • mRNA

  • SD

    mRNASD SD

    SD

  • mRNASD SD

    SD

    1 2 3

  • SD

  • SD

  • SD

  • ?

  • :Mycoplasma genitalium 482

    580.070 bp 482

    RNA 12 470

    . 5.3%. 8.0%. 45.0%V. 13.0%V. 16.6%VI. 8.2%

  • ONII ( 1999)

    X

    .

    (Mbases)

    o

    Saccharomyces cerevisiae 12.069 6.294 3.089 63%Escherichia coli - 4.639 4.289 2.656 62%

    Mycobacterium tuberculosis (strain -H37Rv) + 4.447 4.402

    Bacillus subtilus + 4.214 4.099 ~2.320 ~58%

    Synechocystis sp. - 3.573 3.168 1.402 48%

    Archaeoglobus fulgidus 2.178 2.471 1.193 44%Haemophilus influenzae - 1.830 1.850 1.015 58%

    Methanobacterium thermoautotrophicum 1.751 1.855 816 44%Pyrococcus horikoshii (shinkaj) 1.738Helicobacter pylori - 1.667 1.590 907 57%

    Methanococcus jannaschii 1.664 1.692 776 46%Aquifex aeolicus (strain VF5) - 1.551 1.554

    Borrelia burgdorferi - 1.230 1.256 499 58%

    Treponema pallidum - 1.138 1.041

    Rickettsia prowazeckii B- 1.112 840 526 62%Chlamydia trachomatis - 1.042 896

    Mycoplasma pneumoniae + 0.816 677 333 49%

    Mycoplasma genitalium + 0.580 488 324 69%

    = , = (Gram+ -) =. . ( ) http://www.ncbi.nlm.nih.gov/PMGifs/Genomes/micr.html