Biological Sequence Analysis Biological Sequence Analysis Project PresentationProject Presentation

Project2)

Using HMMER to Search Homologues Protein Family

2002-20631

Tutorial Execution to Understand HMMER

Install Linux -> Not Complete

Trial to Dos version Dowonload from HMMER Homepagehttp://hmmer.wustl.edu/

After downloads, creat folder at C:hmmer and file is unzipped.

Get multiple sequence alignment of a protein domain(protein sequence family) from Pfam databaes

http://pfam.wustl.edu/

http://pfam.wustl.edu/http://pfam.wustl.edu/

My interesting protein family is Aminoacyl-tRNA Synthetase(ARS)

What is Aminoacyl-tRNA Synthetase?

ARS domain search from Pfam database

HMM construction with hmmbuild

HMM calibration with hmmcalibrate

Biological Sequence Analysis Project Presentation