DNA RESEARCH 1, — Supplement, 47-56 (1994)

Prediction of the Coding Sequences of Unidentified Human Genes.I. The Coding Sequences of 40 New Genes (KIAA0001-KIAA0040)

Deduced by Analysis of Randomly Sampled cDNA Clones fromHuman Immature Myeloid Cell Line KG-1 (Supplement)

Nobuo NOMURA,1* Nobuyuki MIYAJIMA,2 Takashi SAZUKA,2 Ayako TANAKA,2 Yutaka KAWARABAYASI,2

Shusei SATO, 2 ' 3 Takahiro NAGASE,2 Naohiko SEKI,2 Ken-ichi ISHIKAWA,2 and Satoshi TABATA2'3

Institute of Gerontology, Nippon Medical School, Nakahara-ku, Kawasaki, Kanagawa 211, Japan,1 KazusaDNA Research Institute, Chuou-ku, Chiba 260, Japan,2 and Department of Biology, Faculty of Science,

Nagoya University, Chigusa-ku, Nagoya 464, Japan3

(Received 30 October 1993)

Sequence features of the predicted coding regions for40 unidentified human genes. Gene numbers are givenon the left side. The horizontal scale represents aminoacid residues from the N-terminus. Occurrence of hy-drophobic amino acid residues is indicated by blue ver-tical bars (long: aromatic, short: aliphatic) and thatof hydrophilic by either green (Arg, Lys and His: long,medium and short upward bars; Glu and Asp: long andshort downward bars) or red vertical bars (long: amides,short: alcohols). Pro is shown by black bars and the po-sitions of Ala, Gly and Cys were left unmarked. Abovethe lines of amino acid residues, the regions that showedsimilarities to known genes are indicated by horizontallines with arrowheads at both ends, the location of pro-tein motifs that matched those in the PROSITE motifdatabase by black blocks, and significant hydrophobic

regions that were identified by the methods of Engelmanet al.1 and of Kyte and Doolittle2 by red blocks. Abbre-viations used are; B, bovine; Ch, Chinese hamster; D,Drosophila melanogaster, H, human; M, mouse; N, Neu-rospora crassa; P, pig; R, rat; Sc, Saccharomyces cere-visiae.

References

1. Engelman, D. M., Steize, T. A., and Goldman, A. 1986,Identifying nonpolar transbilayer helices in amino acid se-quences of membrane proteins, Annu. Rev. Biophys. Bio-phys. Chem., 15, 321-353.

2. Kyte, J. and Doolittle, R. F. 1982, A simple method fordisplaying the hydropathic character of a protein, 157,105-132.

To whom correspondence should be addressed. Tel. +81-44-733-5230; Fax. +81-44-733-5192

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48— Supplement Prediction of Unidentified Human Genes [Vol. 1,

KIAA0001

0002

0003

0004

26.3% of Angiotens-m II receptor (B)

TYR PHOSPHO SITE

, 1 I III I . I I ,, I I . ! ,

I 1 "17 i

30.6% of T-complexpolypeptide 1(H)

EVKASP PROTBAS

l\iHfr If

40.8% officolin (P)

FIBRIN AGC DOMAIN

99.7% of TAF(H)

TYR PHOSPHO SITS

0005

II TIT ' T ' Hllll HI1,200 1.300

LSUCINE ZIPPER

TYR PHOSPHO SITE ATPGTPA

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No. 1] N. Nomura et al. Supplement — 49

32.4% of vav oncogene (H) -)

0006

- 26.2% of Phospholipase C (ft)

} 20.2% of Guanine nucleotide releasing protein (R)

TYR PHOSPHO SITE

400

TYR PHOSPHO SITE

OOO7

| I ' mi1, , , T I | •,.1 I

iiiin|i iilliiJifn! NIMII|II I,< I I

0008

i r' r-| ™ "ii f 'f 'ft vj P ' i l mmpii i M \

[i»li"iii||in|ny

0009

in; rirzoo

OO1O

400 500{ 31.6% of HPVE6-associatedprotein(H)

TYR PHOSPHO SITE

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50— Supplement Prediction of Unidentified Human Genes [Vol. 1,

0011

TBBTA REPEATS

M. | IT[

CBETAMPtATS

0012 20.2%ofToll(D)

TYR PH0SPH0 SITS

0013

26.2%ofrhoGAP(H)

n1

TYR PHOSPH0 SITS

0014

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No. 1] N. Nomura et al. Supplement — 51

0015

<—25.9% of Protein phosphatase 2ca (H) —

0016

0017

H1 jn^\\f

0018

TYR PHQSPHO SITE

*^H _LL

37.4% of tre oncogene(H)

0019

0020 TYR PBOSPBO SITE

300

PROKAR LIPOPROTBIN

- * —

^fU.

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52— Supplement Prediction of Unidentified Human Genes [Vol. 1,

0021

TYR PHOSPHO SITE

33.4% ofCyritestin(M)

IHC PR0TKAS6

0022

0023

i. .di u,,.LL

94.8 % of can oncogene (H)

HMH

ra PHOSPHO SITE

iil|lil|liiiJlii[|il[|l|il|llll|'ii,ii|lili[lN' I"'1'

J, ..j .|.u ,L 1 . 1 . . . . •• I . . . i . . L . . • „ . !

ii wj in 'I IH I||I|[HUI MH IIIIII|HI| urn mum l|i|i ii J i .Li 11

V ! ' Ii|i ii Ji .Li 1

V ! ' I

y i i>| »|ii|JU |i «|i i Htm i; I. ii >| I»n|i> |i ii ii H limn miw MI 11 mi ni| h mi BB i H M ^ » ' »fr' H H H N

u| n m •• H | • | II|J ii| | n P| I|m H > H ; . NPNH'UJ ^niiu

0024

97.3 % of Phosphatidylserine synthase (Ch)

TYR PHOSPHO SITS TYR PHOSPHO SITK

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No. 1] N. Nomura et al. Supplement - 53

0025 TYR PHCSPHO SITE

TYR PHOSPHO SITE

0026 IEVC1SEIW.

002? TYR PHOSPHO SITE PROKAR LIPOPROTTEIS R1BOS0MALS14 PROKAR LIPOPROTTEIS

0028 41.1% of Mitochondria! le-ucyl Into syntasv (N)

A4 TRNA LICASEI CYTO CHRONB C

002S

1..J.J. J.J.J.1 l| Ijfl

I I T r l

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54— Supplement Prediction of Unidentified Human Genes [Vol. f,

0030

45.6% ofMCM2 (Sc)

TYR PHOSPHO SITE

iwi'ki'W'IliPFPII W

100

0031

f 39% of Elongation factor 2 (H)

ATP CTPA TYR PHOSPHO SiTE

TYR PHOSPHO SITE

TYR PHOSPHO SITE

34. 7 % ofRCCI(H)

0032 RECEPTOR CYT0K1NES 2 "Ccl 2 RCCI2 CI2 PROKAR LIRCCI2 PROKAR LIP0PR0TE1N

400 S00

37.6% of HPVE6-associatedprotein(H)28.3%ofKIAA0010(H)

TYR PHOSPHO SITE

y* W* V*^fr»f ^ T

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No. 11 N. Nomura et al. Supplement — 55

0033 TYR PHOSPHO SITE

0034 99.8% of Clathrin heavy chain (R)

*fI^BU^I

TYR PHOSPHO S/TE TYR PHOSPHO SITE

TYR PHOSPHO SITE

||lylj|l||l

TYR PHOSPHO SITS

0035

72. 7% of Nucleolarprotein Noppt40(R)

,;i«l,iii,y,lJ|ll,llll|iiii||l , I Jm

0036 TYR PHOSPHO SITE

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56— Supplement Prediction of Unidentified Human Genes

003? PROKAK L1P0PR0TE1N

55.6% ofAdenylate cyclase U (R)

«»••• 'i H I ' , " . p

TYR PHOSPHO SITE TYR PHOSPHO SITE OSPHO STYR PHOSPHO SITS TYR PHOSPHO SITE

,.^, , , M , .u , , ,„•„• .W, L ,|UH^

PROKAR L1PQPRDTEIN TYR PHOSPHO SITE PROKAR UPOPROTEIN

GUASYUTE CYCUSES TYR PHOSPHO SITE

TYR PHOSPHO SITE

I.. I.I I... I... I. ll.'I'lifi ' ¥ h V

31.9% of Initiation factor 4B (H)0038 TYR PHOSPHO SITE RNP f

0039 TYR PHOSPHO SITE

|mi|lill|li|iiyil|iLiiJi

NI'III ii™«'i'ik i ikii

0040 TYR PHOSPHO SITE

. | . l . , H .1 ||i

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