........................................... 1

....................................................................................................... 4

................................................................... 10

........... 15

........................................................................................................... 20

............................................................................................................................. 25

...................................................................................... 30

........................................................................ 34

“ ” ................................................................... 40

................................................. 45

AI AI .......................... 48

.............................................................................. 51

................................................................................................................... 56

............................................................... 60

.................................................. 66

........................................................................ 70

T ................................................................... 75

................................................. 80

pH .................................................................................... 86

........................................................................... 89

................................................. 92

........................................................................................... 98

................................................. 101

...................................................................................................... 106

PGA ................................................................................................... 109

....................................................... 112

........................................................ 116

........................................................................................... 122

C

Synergistic Antioxidant Effects between Catechins and VitaminC ................................. 126

PGA

PGA

PGA Ca(OH)

PGA

α

PGA γ

PGA

PGA Ca(OH)

PGA

99%

PGA Ca(OH)

3

PGA

20 mL 4

PGA Ca(OH) PGA

Ca(OH)

0 2 g/L

1

PGA Ca(OH) PGA Ca(OH)

20 mL 3

PGA(0 02 g)

Ca(OH) 0 01 g 0 001 g 0

0001 g

20 mL 2

PGA PGA

PGA

PGA

Ca(OH)

PGA

PGA

PGA

PGA

Ca(OH)

PGA

PGA

Ca(OH)

Ca(OH)

pH PGA

PGA

PGA

PGA

PGA Ca(OH)

PGA

Ca(OH)

PGA

PGA

PGA

1-3

otemae-hs ed jp

“ ”

chem utsnomiya-u ac jp

2008

2009

Vol 53 No 4

271 274

poly-glu com

mizusyoli com

8

.

100mL

10 , 8

8 .

2

8 1 1 20

, 3

,

.

9

100μL 37

8

1

10μL ( 0.006g

10μL) 2 9

70μL ( 0.3g) 3

9 3M

8

37

1mL

1:1 2:1 3:1 1:2

4

1 4 7

3M

1

100 50 10 5 1

1

100g

3 0.5g

0.006g 1

2

2

10μL

70μL

M

4

M

.

(

) ( )

18 20

( )

( )

pH

Jica,

https://www.jica.go.jp/aboutoda/ikega

mi/01/

JOMF,

http://www.jomf.or.jp/report/kaigai/2

2/2.htm

Water Life,

!?

http://water-life.info/?p=844

ZUCCA,

http://www.yasaiyasai.com/name/k/k

arashina/

2008,

https://www.kewpie.co.jp/sakusankin

mizkan,

http://www.mizkan.co.jp/k-plus/infor

mation/osu/make.html

F( )

F

A ( )

B

1 A 21.3g B 8.5

2 4.48g

320μL

3 2 A

4 3 B 30

4 90

1 A 100

2 A 200

3 A 100

4 A 200

5 3

3cm

( ) 1g

1 10

1

4 1

1

2 1

3 1

4

5

5

F( )

F

A ( )

B

( 40 100 200

300)

10.23

3.3

1.1

2.8

0

2

4

1 2 3 4 5

1g

1

(1 4) 320μL

À 100

40

100

200

300

1.12 À 100

3cm

1g

1

10

* 2 6 2

2

4 2 1 3 5 2 4

1 4.8cm 9

2. 4.4cm 10

3. 6.6cm 11

4. 4.2cm 12

5. 3.1cm

13

9.9cm 14

( 3)

1( 4)

2( 5)

3( 6)

4( 7)

5( 8)

2.1

3.9

2.0

5.3

1.8

0

2

4

6

1 2 3 4 5

()

1

4

3

4

2

2

5

2 1

TA

24

1 2

, 6

80 20

2ml 2ml

0.5ml 70 80

10

2

1 2

1

68 75

60 70 80

2

3

95

0.05mol 0.01mol 4

2

80

20 1 1 1 10 1 20

1 50 4

4

80 20

1 50

20

2

1/50

80

20

2

https://www.deodor.co.jp/bousihou-kyo

udo.htm

2012

202 203

2005

38 39 44 45

2014 Shokuten life-

http://shokutenlife.com./2014/04/08/3

262.html

evangelist@nikkol 2011 chemical-navi

http://www.chemical-navi.com/column

/foods

2010 1

www.asama-chemical.co.jp/TENKAB/

YUKAWA14.HTM

2017 taberugo.net/21

C

Synergistic Antioxidant Effects between Catechins and VitaminC

C . C

DPPH

C 0.02

g/L~0.08 g/L C

C

1

2013 C

C

C

90 100 C

2

C

C K

200 g 3

C

K DHC C

C

C K

C

C

C

2 DPPH

2

2-1.

1,1- -2-

DPPH

520 nm

( 1)

C C

K 200 g 3

50 g

1

2-2.

Tris-HCl 200 mM, pH 7.4 25 mL,

10 mL DPPH (0.2 mg DPPH/mL

A

A4.5 mL 0.5 mL

20

Shimadzu UV-1700 520

nm

= !"

× 100

α=DPPH

β=DPPH

A 1.00 g/L

B 0.500 g/L

C C 1.00 g/L

D C 0.500 g/L

E C 0.100 g/L

F C 0.0500 g/L

G C 1.00 g/L 1.00 g/L

H C 1.00 g/L 0.100 g/L

I C 0.0200 g/L

J 0.0200 g/L

K 0.0200 g/L C 0.0200 g/L

L 0.0200 g/L C 0.0200 g/L

M 0.0200 g/L C 0.0200 g/L

N 0.0200 g/L C 0.200 g/L

3

2 Fig.1

0.788

70%

2 Fig.2 Fig.3

C

3 Fig.4

0.991

Fig.1

(mL)

(%)

A 0.50 0.256 67.5

B 0.50 0.264 66.4

C 0.50 0.215 72.7

D 0.50 0.251 68.1

E 0.50 0.234 70.3

F 0.50 0.428 45.7

G A 0.25

C 0.25 0.224 71.6

H A 0.25

E 0.25 0.428 71.9

Fig.2

Fig.3

Fig.4

4

C

C

1

70%

1 DPPH

C

1 2

C

DPPH 4.5 mL

C 0.02 g/L~0.08

g/L

30% 60%

C

1 C

0.1 g/L 70%

70%

3

C

1

C

C

C

C 0.2 g/L

1

2

0.2 g/L

C

(g/L)

(mL)

(%)

I C

0.02 0.50 0.990 0.10

J 0.02

0.50 0.840 15.2

K I 0.25

J 0.25 0.848 14.4

L I 0.25

J 0.25 0.850 14.2

M I 0.25

J 0.25 0.878 11.2

N I 0.25

J 0.25 0.878 11.4

33.8

48.1 51.856.6

0

10

20

30

40

50

60

70

0.02 0.04 0.06 0.08

(%)

C (g/L)

30.3

40.4

53.460.7

0

10

20

30

40

50

60

70

80

0.02 0.04 0.06 0.08

(%)

(g/L)

5

5-1.

DPPH

C

C 0.02 g/L~0.08 g/L

C

70%

C

5-2.

3 1

DPPH C

2 20

2

C

3 2

6

2009

2015

―

http://www.naro.affrC.go.jp/org/nfri/y

akudaChi/manual/2-4.html

2013

Mark S. M. Alger (1997). Polymer sCienCe

diCtionary. Springer.

ISBN 0-412-60870-7

7