The Broucek MissionResearcher: Allison Broucek

Purpose• Inspiration: Relevance and passion• Background: Curiosity about plant based diets• Journal of Human Nutrition and Dietetics• Influence of self-selected vegetarian diets on blood

lipids• Journal of Nutrition and Metabolism• Effects of vegetarianism on elderly

• Importance: American based diseases and conditions• Correlations?• Dietary intervention?

Study Program• 30 Subjects• 22 Females/8 Males• 21 to 68

• Recruitment• Trine faculty/staff• Local residents

• 8 Week Study• Experimental: Lacto-Ovo Vegetarian Diet – Cutting plan

HypothesesNull Hypothesis – H0There is no difference between the experimental subjects who followedthe Lacto - Ovo Vegetarian diet and the control subjects who made no change.

Alternative HypothesisThere is a significant change within the experimental group compared to the control group when testing: Insert testing category.

Methods• Interest Meetings/Emails/Flyers• Random recruitment and placement

• Informed Consent/Photo Consent• Pre Assessment Questionnaire• Health, drug, socioeconomic history, daily lifestyle, food frequency, etc.

• Pre and Post Testing• Weight/height/BMI/body fat percentage• Blood pressure/RHR• Cholesterol profiles/glucose

Methods• Dietary Recalls• Control: Written servings• Experimental: MyFitnessPal

• Weekly Meetings and Topics• Ex. Costs Differences between Healthy Foods and Convenience Foods/Grocery Shopping

• Communication• Control: Via email• Experimental: Email, phone, in person

Methods• Food Demonstrations• Mexican/Italian/Nordic/Japanese

• Weekly Updates:• MyFitnessPal group• Personal updates via email/phone/in person

• Resource Packets• Alternative protein options• User friendly vegetarian meals• Major food groups and healthy options• Beginner vegetarian cookbook

Statistical AnalysisE Pre Sys E Post Sys E Pre Dia E Post Dia

139 120 85 72E Pre RHR E Post RHR E Pre LBS E Post LBS

70 58 133.4 132E Pre BMI E Post BMI E Pre BF % E Post BF %

24 23.8 38.1 37.4E Pre TC E Post TC E Pre HDL E Post HDL

165 160 66 59E Pre LDL E Post LDL E Pre TRG E Post TRG

61 73 187 137E Pre n-hdl E Post n-hdl E Pre tc/hdl E Post tc/hdl

98 101 2.5 2.7E Pre GLU E Post GLU

115 79

Experimental Subject # 1

Pre/Post Test Data

Differences between pre and post test Two Sample T-Test P Value < 0.05

Significant Findings• Weight Loss: P =

0.0.0011933C LBS Diff E LBS Diff t-Test: Two-Sample Assuming Unequal Variances

-1 1.4-0.2 2   C LBS Diff E LBS Diff5.4 3.2 Mean 0.393333333 5.560.2 2.2 Variance 14.44352381 21.20114286-3 4 Observations 15 15

2.2 2.4 Hypothesized Mean Difference 0 0.8 6.6 df 27 6.4 13.6 t Stat -3.35165103 6.8 3.6 P(T<=t) one-tail 0.001193397 0 4.4 t Critical one-tail 1.703288446

-2.2 5 P(T<=t) two-tail 0.002386795 1 4 t Critical two-tail 2.051830516

-7.1 17Weight (Pounds)-4 3.2

0.6 10.8

Resting Heart Rate: P = 0.004611201 C RHR Diff

E RHR Diff t-Test: Two-Sample Assuming Unequal Variances

8 12 -4 -7   C RHR Diff  E RHR Diff -2 1 Mean -3.666666667 6.2

-30 -2 Variance 115.6666667 68.88571429 7 1 Observations 15 15 -4 7 Hypothesized Mean Difference 0 3 5 df 26 0 2 t Stat -2.812912737

-25 21 P(T<=t) one-tail 0.004611201 -7 12 t Critical one-tail 1.70561792 -2 0 P(T<=t) two-tail 0.009222402 -4 1 t Critical two-tail 2.055529439 2 13

Resting Heart Rate 7 5 -4 22

Body Mass Index: P = 0.001509476

C BMI Diff

E BMI Diff t-Test: Two-Sample Assuming Unequal Variances

0 0.2 0 0.3    C BMI Diff  E BMI Diff 

0.9 0.6 Mean 0.1 0.886666667 0 0.3 Variance 0.35 0.524095238

-0.1 0.6 Observations 15 15 0.3 0.3 Hypothesized Mean Difference 0 0.2 1.1 df 27

1.1 2 t Stat -

3.258794695 0.9 0.5 P(T<=t) one-tail 0.001509476 0.1 0.7 t Critical one-tail 1.703288446 -0.3 0.7 P(T<=t) two-tail 0.003018953 0.1 0.8 t Critical two-tail 2.051830516 -1.2 2.7

Body Mass Index -0.7 0.7 0.2 1.8

Significant Findings• Glucose: P = 0.044912216

C GLU Diff E GLU

Diff t-Test: Two-Sample Assuming Unequal Variances 50 36 0 18    C GLU Diff  E GLU Diff 

32 22 Mean -1.933333333 11 -34 10 Variance 449.352381 363.2857143 -15 -5 Observations 15 15 6 5 Hypothesized Mean Difference 0

-23 -4 df 28 5 33 t Stat -1.757144638 4 -24 P(T<=t) one-tail 0.044912216

-12 1 t Critical one-tail 1.701130934 -6 -2 P(T<=t) two-tail 0.089824432 0 15 t Critical two-tail 2.048407142 -8 4

Glucose -4 4 -24 52

ConclusionHypotheses H3, H4, H6, H13: Reject H0

Hypotheses H1, H2, H5, H7. H8. H9, H10, H11, H12:Failure to reject H0

Discussion• What causes cholesterol?• Client constructed needs• Individual significant findings

“Cholesterol is not considered a nutrient of concern for overconsumption“ ~Dietary Guidelines Advisory Committee 2014

“Estimate of only 20 percent of a person’s blood cholesterol levels actually come from their diet”~Dr. Steven Nissen/Cleveland Clinic

Reflection• Control: No dietary recalls• Psychological effect

• Further research• Causes of cholesterol• A1c Plasma Glucose• Longer duration of time

Acknowledgements• Julia D. Just, RD.

• 8 Week dietary plan• Kent Stucky and Deborah McHenry (Trine University)

• Donations for The Broucek Mission• John White (Cameron Hospital)

• Alere Cholestech LDX Analyzer• Amy Oberlin (Angola Herald Republican)

• Newspaper article• Krista Miller (Steuben County YMCA CEO)

• Interest Meetings • Karen Heldt and Lori Miedema

• Alere Test Cassettes• Dr. Rudy A. Kachmann, MD

• Support and Motivation • Bon Appetit

• Food Demonstrations• Susan Anspaugh, Catherine Swick

The Broucek Mission• "To inspire and encourage others, no matter what

age, background, or health history, to make a change, in order to improve the health and overall quality of their life. "

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