禽流感與人畜共通疾病研討會

禽流感與人畜共通疾病研討會

第四屆沙門氏菌暨其他細菌性人畜共通疾病研討會

Fourth National Symposium on Salmonellelosis

and other bacterial zoonosis

議程及論文集摘要

Final Program and Abstract

時 間：民國98年9月12日 (星期六)

主辦單位：國立嘉義大學

生命科學院 微生物與免疫學系/生物醫藥科學研究所

農 學 院 獸醫學系 動物科學系

農業委員會 動植物動植物防疫檢疫局

地 點：國立嘉義大學蘭潭校區圖資大樓演講廳

（嘉義市學府路300號）

禽流感與人畜共通疾病研討會

第四屆沙門氏菌暨其他細菌性人畜共通疾病研討會

時間：民國98年9月12日 (星期六)

主辦單位：國立嘉義大學 生命科學院 微生物與免疫學系/生物醫藥科學研究所

農 學 院 獸醫學系 動物科學系

農業委員會 動植物動植物防疫檢疫局

地點：國立嘉義大學蘭潭校區圖資大樓演講廳（嘉義市學府路300號）

議程表

時 間

內 容

主持人

演講人

08:30-09:00

報到 (Registration)

09:00-9:20

開幕典禮(Opening remarks)

校長、院長

09:20-9:50

專題演講

家禽流行性感冒與人類的關係

張伯俊

陳秋麟

國立嘉義大學

09:50-10:20

專題演講：以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒

陳秋麟

張伯俊

國立中興大學

10:20-10:40

Break

禽流感與其他細菌人畜共通傳染病

10:40-11:10

專題演講:

禽流感之基因重組桿狀病毒疫苗之開發

蘇耀期

劉宏仁

國立屏東科技大學

11:10-11:30

乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於Methicillin與Vancomycin之抗藥性情形

劉宏仁

蘇耀期

國立嘉義大學

11:30-11:50

Necrotizing fasciitis and mastitis associated methicillin resitance Staphyloccus aureus

張簡志炫

嘉義基督教醫院

11:50-13:00

Lunch (Poster session)

流行病學 與治療

13:00-13:15

犬隻沙氏桿菌分子流行病學之調查

余章游

廖明輝

國立屏東科技大學

13:15:13:30

High mobility group 1 (HMGB1) protein as the therapeutic target for severe sepsis and septic shock

金立德

國立嘉義大學

13:30-13:45

嘉義地區沙門氏菌在水禽幼雛及蛋膜感染

金立德

余章游

國立嘉義大學

13:45-14:00

Salmonella Virchow質體增加在THP-1細胞中存活能力及Salmonella Virchow SGI-2之演化

李永茹

國立嘉義大學

14:00-14:20

合照

14:20-15:00

Break (Poster session)

致病機制與遺傳 (Pathogenesis and Genetics)

15:00-15:15

Immunity of Chicken to Salmonella enterica serovar Enteritidis infection

鄧景浩

朱紀實

國立嘉義大學

15:15-15:30

A constitutively mannose-sensitive agglutinating Salmonella enterica serovar Typhimurium LB5010 strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes

葉光勝

臺北醫學大學

15:30-15:45

Why Salmonella enterica serotype Choleraesuis is more invasive than serovar Typhimurium?

朱紀實

邱政洵

長庚林口醫院

15:45-16:00

Identification and characterization of Escherichia coligenes epidemiologically associated with urinary tract iInfections

鄧景浩

國立成功大學

16:00-16:30

Panel Discussion

余章游、蘇耀期、陳國隆、朱紀實、葉光勝

Lecture section

1. Convergent evolution of Salmonella typhoid agents -------- P

劉樹林

北京大學、哈爾濱醫科大學、CUniversity of Calgary

2. 家禽流行性感冒與人類的關係

陳秋麟

國立嘉義大學獸醫學系

3. 以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒

張伯俊1、謝快樂2、沈瑞鴻2

1國立中興大學微生物暨公共衛生學研究所, 2國立中興大學獸醫學系

4. 禽流感之基因重組桿狀病毒疫苗之開發

劉宏仁

國立屏東科技大學 生物科技研究所 獸醫學系

5. 乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於Methicillin與Vancomycin之抗藥性情形

蘇耀期、李研懷

國立嘉義大學獸醫學系

6. Methicillin-resistant Staphylococcus aureus associated necrotizing fasciitis and mastitis

Chih-Hsuan Changchien1, 2, Wan-Lin Chen2, and Chishih Chu1*

Department of Microbiology and Immunology, National Chiayi University1; Department of Plastic and Reconstructive Surgery, Chiayi Christian Hospital2

7. 犬隻沙氏桿菌分子流行病學之調查

1劉紋紋 2陳玄峰 1盧明志 1廖明輝

1國立屏東科技大學獸醫學系 2國立屏東科技大學生物科技研究所

8. High mobility group 1 (HMGB1) protein as the therapeutic target for severe sepsis and septic shock

Li-Te Chin

國立嘉義大學微生物與免醫學系

9. 嘉義地區沙門氏菌在水禽幼雛及蛋膜感染

Chang-You Yu,*§ Jiang-Liang Lin,* Chishih. Chu,* Yao-Chi Su,§ Shu-Wun Chen, * Pei-Chun Tu,

§Department of Veterinary Medicine, *Department of Microbiology and Immunology, National Chiayi University

10. The change of Salmonella genomic island and R plasmid-mediating survival in THP-1 cells for Salmonella enterica serovar Virchow

Yung-Lu Lee1, Chien-Shun Chiou2, Chishih Chu1,

1Graduate Institute and Department of Microbiology and Immunology, National Chiayi University, 2The Central Branch Office, Center for Disease Control

11. Immunity of Chicken to Salmonella enterica serovar Enteritidis infection

Chishih Chu1, Kuo-Lung Chen2, Chang-You Yu3

Department of Microbiology and Immunology1, Department of Animal Sciences2, Department of Veterinary Medicines3, National Chiayi University

12. A constitutively mannose-sensitive agglutinating Salmonella enterica serovar Typhimurium LB5010 strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes

1吳冠勳, 2王克銓, 3許元勳, 3葉光勝

1台北市立萬芳醫院小兒科, 2臺北醫學大學醫學科學研究所,

3臺北醫學大學醫學系微免學科

13. Why Salmonella enterica serotype Choleraesuis is more invasive than serovar Typhimurium?

K.-Y. Huang1, I.-H. Wang1, L.-C. Wang1, C.-H. Chiu1,2

1Graduate Institute of Basic Medical Science, Chang Gung University College of Medicine and 2Department of Pediatrics, Chang Gung Children’s Hospital, Chang Gung University College of Medicine

14. Identification and Characterization of Escherichia coli Genes Epidemiologically Associated with Urinary Tract Infections

Bin-Hsu Mao, Wen-Jun Huang, and Ching-Hao Teng

Institute of Molecular Medicine, National Cheng Kung University School of Medicine, Tainan city, Taiwan

Poster section

1. Differences in characteristics between Streptococcus agalactiae fish and human isolates

Pei-Yu Huang1, Yu-Hsin Chang1, Feng-Ting Chang1, Jiun-Nun Hou2, Chishih Chu2, Chun-Che Chen1

Graduate Institute of Aquatic Biosciences, National Chiayi University1

Department of Microbiology and Immunology, National Chiayi University2

2. Plasmid, antibiotic susceptibility and pulsed field gel electrophoresis (PFGE) analysis of Salmonella Pullrum

Yi-Chun Chen1, Shu-Wun Chen1, Shu-Lin Liu2 and Chishih Chu1

1 Department of Microbiology and Immunology, National Chiayi University, Taiwan, ROC and 2Department of Microbiology, Peking University Health Science Center, Beijing, PROC

3. Differences in antimicrobial susceptibility, resistance genes. plasmid types between blood and respiratory Escherichia coli isolates

Yi-Lin Tsai1, Nuo-Shian Wang1, Yi-Hsuan Chen1, Shu-Wun Chen1, Jain-Shing Liu2, and Chishih Chu1

Department of Microbiology and Immunology, National Chiayi University1

Tainan Hospital, Department of Health, Executive Yuan, R.O.C.2

4. Antimicrobial resistance to erythromycin and fluorioquinolone and their resistance genes in Streptococcus agalactiae

Yi-Rou Wang1, Jiun-Nun Hou1, Ting-Yu Lin 1, Mei-Hui Wang2, Ying-Shiang Wang2, Chishih Chu1

Department of Microbiology and Immunology, National Chiayi University1; Department of Pediatrics, Chiayi Chang-Gung Memorial Hospital2

5. Genetic variations and antimicrobial susceptibility of clinical Salmonella enterica serotype Stanley isolates in 2004 - 2007 from Taiwan

Li-Ting Sun1, Dvi-Wei Fan1, Shu-Wun Chen1, Chien-Shun Chiou2, Chishih Chu1

Department of Microbiology and Immunology, national Chiayi University,1

The Central Branch Office, Center for Disease Control, Taichung, Taiwan2

6. 乳牛披衣菌感染之疫情探討

陳賢文、余章游

國立嘉義大學獸醫學系

7. Pathogenicity and antibiotic Resistance in Streptococcus iniae

Feng-Ting Chang, Bin-Hong Hsu, Che-Chun Chen

Department of Aquatic Biosciences, National Chiayi University

8. 山羊流產披衣菌

李志展

國立嘉義大學獸醫學系

9. 嘉南地區種禽場沙門氏菌疫情調查

申國榮、郭慧蓮、辜啟瑞、吳晟瑋、蔡宗展、陳秋麟、余章游

國立嘉義大學獸醫學系

10. The protective effect of β-glucan from different sources on Salmonella Enteritidis invasion in chicks

May-Ling Ho*, Ling-Ping Huang, Tsai-Tzu Chen, Kuo-Lung Chen

Department of Animal Science, National Chiayi University

劉樹林 MD & Ph

微生物學教授

電話: 86 451 8666 9150 (辦公室); 86 451 8750 6380 (秘書)

E-mail: slliu@ucalgary.ca, slliu@bjmu.edu.cn學歷

1982

學士哈爾濱醫科大學

1985

碩士北京醫科大學

1990

博士日本岐阜大學

工作經歷Work Experience

2008.03 –Present

哈爾濱醫科大學基因組中心教授，主任，博士研究生導師

2002.02-Present

北京大學醫學部教授，博士研究生導師

1.

2.

3.

4.

1998.10-Present

Adjunct Research Professor, Department of Microbiology and Infectious Diseases, University of Calgary, Faculty of Medicine, Calgary, AB, Canada.

Research: Genomic divergence and evolution of Salmonella.

1996.12-1998.9

Research associate, Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, Calgary, AB, Canada.

Research: Bacterial genomic evolution.

1992.06-1996.11

Research associate, Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.

Research: Construction and comparison of physical maps of Salmonella species.

1990.03-1992.05

Postdoctoral fellow, Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.

Research: Physical mapping of S. typhimurium LT2, S. enteritidis, S. paratyphi B.

雜誌編委Journal Editorship

Member of Editorial Board of Journal of Microbiology and Immunology

Member of Editorial Board of International Journal of Genetics

稿件審閱 (Manuscript review for the following journals)

Journal of Bacteriology (ASM, USA)

Microbiology (France)

Canadian Journal of Microbiology (Canada)

Genome Biology (UK)

Emerging Infectious Diseases (USA)

中華微生物學和免疫學雜誌Chinese Journal of Microbiology and Immunology (China)

Nucleic Acid Research (USA)

Journal of Clinical Microbiology (ASM, USA)

Functional and Integrative Genomics (Germany)

Hereditas (Sweden)

International Journal of Immunogenetics (UK)

Journal of Microbiology and Immunology (中華微免英文版，China)

中華醫學雜誌Chinese Journal of Medicine (China)

中國生物化學和分子生物學報

北京大學學報（醫學版）

Journal of Life Sciences (China)

BMC Microbiology (UK)、

BMC Genomics (UK)、

BMC Bioinformatics (UK)

Infection and Immunity (ASM, USA)

Cell Biology International (UK)

Veterinary Microbiology (Elsevier)Applied and Environmental Microbiology (ASM, USA)

Cancer Letters (Elsevier)

Genomics (Elsevier)

國際遺傳學雜誌

Genome Research

Journal of Biomedicine and Biotechnology

專利

1. A biological method for production of enterodiol. Application number: 200810104215.2

2. A novel method for construction of tumour vaccines based on attenuated Salmonella. Application number: 200710179692.0

發表論著

I. 原始論文 (2007-2009

1. Qing-Hua Zou, et al., Molecular characterization of a novel prophage of Salmonella paratyphi C. Under review by J Bacteriol.

2. Fang Chen, et al., mutL regulates genetic stability through conversion between two allele states: a role in bacterial evolution at the population level. Under review by Genetics.

3. Wei-Qiao Liu, et al., Genome comparison of Salmonella typhimurium DT104 strains: multi-resistance not exclusively determined by SGI-1. Under review by FEMS Microbiology Letters.

4. Fang Chen, et al., 2009. A Genome Map of Salmonella enterica serovar Agona: Numerous Insertions and Deletions Reflecting the Evolutionary History of a Human Pathogen. FEMS Microbiology Letters. 293: 188-195.

5. Wei-Qiao Liu, et al., 2009. Salmonella paratyphi C: diverging from S. choleraesuis and converging with S. typhi. PLoS ONE. 4(2): e4510.doi:10.1371/journal.pone.0004510

6. Ye-Jun Wang, et al., 2008. Two oral HBx vaccines delivered by live attenuated Salmonella: both eliciting effective anti-tumor immunity. Cancer Letters. 263: 67-76.

7. Zheng, X., S.-L. Liu. 2008. The relaxing ori-ter balance of Mycoplasma genomes. Science in China. 51 (2): 1-8.

8. Liu, W.-Q., et al., 2007. Diverse genome structures of Salmonella paratyphi C. BMC Genomics. 8: 290.

9. Gong, J., et al., 2007. Spontaneous conversion between mutL and 6bpmutL in Salmonella typhimurium LT7: association with genome diversification and possible roles in bacterial adaptation. Genomics. 90: 542-549.

10. 汪業軍，侯豔，黃鶴，劉桂榮，劉樹林。2007。沙門菌III型分泌蛋白融合HBx抗原介導的T細胞免疫效果觀察。中華微生物學和免疫學雜誌。27：719-724。

11. 嚴岩，李彤，劉樹林。2007。兩種培養基對銅綠假單胞菌和大腸埃希菌生物被膜厚度的影響。中國實驗診斷學(Chin J Lab Diagn)。11（5）：12-17。

12. 劉桂榮，劉葳嶠，祁丹妮，何曉燕，張鳳民，劉樹林。2006。雞沙門菌基因組物理圖。中華微生物學和免疫學雜誌。26：965-970。

13. 部小霞，Aaron White，王玲，劉樹林。2006。沙門菌細聚合菌毛作為新型載體表達外源基因片段。中華微生物學和免疫學雜誌。26：974-978。

14.

15.

16.

II. 綜述與合著

1. S.-L. Liu. 2007. Physical Mapping of Salmonella Genomes. In “Methods in Molecular Biology”, Heide Schatten and Abraham Eisenstark (Eds), The Humana Press.

2.

3.

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15.

Convergent evolution of Salmonella typhoid agents

Shu-Lin Liu

哈爾濱醫科大學、北京大學醫學部、University of Calgary

To date, over 2500 Salmonella serovars have been known and more than 1400 may cause infections in warm blooded animals. In humans, most Salmonella pathogens cause usually self-limited gastroenteritis, but a few, e.g., Salmonella typhi and S. paratyphi C, cause typhoid, a potentially fatal systemic infection. It is not known whether the typhoid agents have evolved from a common ancestor (by divergent processes) or acquired similar pathogenic traits independently (by convergent processes). Comparison of different typhoid agents with non-typhoidal Salmonella lineages will provide excellent models for studies on how similar pathogens might have evolved. We sequenced a strain of S. paratyphi C, RKS4594, and compared it with previously sequenced Salmonella strains. RKS4594 contains a chromosome of 4,833,080 bp and a plasmid of 55,414 bp. We predicted 4,640 intact coding sequences (4,578 in the chromosome and 62 in the plasmid) and 152 pseudogenes (149 in the chromosome and 3 in the plasmid). RKS4594 shares as many as 4346 of the 4,640 genes with a strain of S. choleraesuis, which is primarily a swine pathogen, but only 4008 genes with another human-adapted typhoid agent, S. typhi. Comparison of 3691 genes shared by all six sequenced Salmonella strains placed S. paratyphi C and S. choleraesuis together at one end, and S. typhi at the opposite end, of the phylogenetic tree, demonstrating separate ancestries of the human-adapted typhoid agents. S. paratyphi C seemed to have suffered enormous selection pressures during its adaptation to man as suggested by the differential nucleotide substitutions and different sets of pseudogenes, between S. paratyphi C and S. choleraesuis.

陳秋麟 教授

國立嘉義大學 農學院  獸醫學系

現　　職：教授兼主任秘書

學　　歷：國立中興大學獸醫學博士(2004畢業)

　　　　　國立中興大學獸醫學碩士(1985/09至1987/06)

　　　　　台灣省立嘉義農業專科學校獸醫科(1968/09至1974/06)

簡要經歷：

　　國立嘉義技術學院畜牧科講師、副教授

　　台灣省政府農林廳畜牧科家畜衛生股長　

台灣省立嘉義農業專科學校獸醫科助教

(A)期刊論文

     1.

 

王璧娟、連塗發、陳秋麟、林高塚。2000。牛蒡對老鼠血糖、血脂質與尿酸的影響。台灣農業化學與食品化學。38(2): 181-183。

2.

陳國隆、陳秋麟、吳建平。2000。不同灌食次數及縮短灌食期間對土番鴨生產肥肝之影響。中華生質能源會誌。19(1-2): 1-9。

3.

龔順榮、張伯俊、陳秋麟、謝快樂。2000。台灣雞源與鴨源分離之敗血性巴斯德桿菌之分子特性。中華民國獸醫學會雜誌。26(3): 233-241。

4.

Chen CL, Chang MS, Lee MS, Shien JH, Ou SJ, Shieh HK 2003, Nucleotide sequences of goose circovirus isolated in Taiwan Avian Pathology 32:165-171.

   5. 陳秋麟、張伯俊、謝快樂、李敏旭、歐繕嘉、陳建弘、沈瑞鴻。2004。應用聚 合

酶連鎖反應檢測水禽中之鵝環狀病毒。台灣獸醫誌。30(1):84-90。

   6. 陳秋麟、張伯俊、沈瑞鴻、龔順榮、李敏旭、陳建弘、謝快樂。2004。台灣鵝

環狀病毒之分子流行病學調查與病毒定量技術。台灣獸醫誌。30(2):156-162。

   7. 吳建平、陳秋麟、蔡秀敏、陳國隆。2004。性別對來航雞隻免疫反應之影響。嘉大農林學報。1：85-96。

   8. 吳建平、陳秋麟、李宗育、陳國隆。2004。菸鹼酸鉻對肥育期豬隻生長、血液及屠體性狀之影響。中國畜牧學會會誌。33(4)：271-277。

9. 陳秋麟、李敏旭、張淑卿、黃郁珳、羅登源。2006。台灣火雞禽痘感染。嘉大農林學報。3：99-107。

10. Chen CL. et al., 2006. Development of a Polymerase Chain Reaction Procedure for Detection and Differentiation of Duck and Goose Circovirus. Avian Diseases 50: 92-95.

11. 張淑卿、龔順榮、施良儒、*陳秋麟。2006。台灣火雞鼻氣管鳥桿菌（Ornithobacterium rhinotracheale）細菌分離及血清學調查。台灣獸醫學雜誌。32：180-185。

12. Lee MS, et al., 2006. Genetic and Pathogenic Characterization of H6N1 Avian Influenza Viruses Isolated in Taiwan between 1972 and 2005. Avian Diseases 50: 561-571.

13. Ching-Ho WANG, et al., 2007. Serological Prevalence of Salmonella Enterica Serovars Enteritidis and Pullorum in Different Types of Chickens in Taiwan During 2005. Taiwan Vet J 33: 14-19.

14. Dan-Yuan Lo, et al., 2007. Appendicular Telangiectatic Osteosarcoma. J Agric For (NCYU). 4: 1-9.

O1

禽流感和人類的關係

陳秋麟 教授

國立嘉義大學獸醫學系

流行性感冒病毒屬於正黏液病毒科 (Orthomyxoviridae family)，根據其核蛋白及基質蛋白的抗原差異分為甲、乙或丙型（Type A,B,C）。禽流感病毒(AIV)屬於甲型（Type A），目前分為高病原性禽流感病毒（HPAI）和低病原性禽流感病毒（LPAI），依國際疫會規定只要是H5或H7不論HPAI或LPAI均需通報，然後依一定程序鑑定後採取既定防疫措施。禽流感病毒冇種表面抗原-血液凝集素 (haemagglutinin, H)及神經胺酸酶 (neuraminidase, N)，能引起特定亞型及免疫反應，目前有16種H亞型 (H1-16) 及 9種N亞型 (N1-9)。禽流感病毒對鳥類的內皮起源 (腸，肺) 之上皮組織的主要受體種類-α2,3連結的唾液酸接受體結合位置（receptor-binding site）有高親合性，因此主要影響禽鳥，對雞隻和火雞具有高致病力，但因病毒會不定時基因突變，衍生新品種，導致原來僅感染禽類的流感病毒，變得可以影響人類；此外，已研究證實人類氣管、支氣管和小支氣管內有一定數目的纖毛細胞同樣帶有較低密度像禽類之接受體，對於與受感染的活家禽、被其糞便嚴重污染的表面或物件有密切接觸的人，感染的風險極高。1997年於香港爆發H5N1，當時受感染的18人中有6人死亡，在流行病學上與活禽市場的高度接觸有關；2003年末再度發生H5N1亞型高病原性禽流感，接著由東北亞、東南亞並傳至蘇聯和歐洲，由於這些突變的流感病毒對人類是全新的病毒，因此曾和病禽或其病源接觸的人導致嚴重病症，自2003年起累計至2009年8月31日止全球共發生440病例，262人死亡。由於這高致病性禽流感H5N1病毒株有特異性能跳躍不同物種類障礙和在人類引起高致死率嚴重疾病，文獻指出A型流感病毒在不同物種間的傳播以及重組曾見於豬、人、鴨及火雞，引起1957及1968年世界大流行的人類流感病毒均含有類似禽類流感病毒的基因片斷，因此，全球仍關注其是否在人類提升另一全世界流行性感冒大流行的可能性，當人（高度風險群）同時感染禽流感與和流行性感冒兩種病毒，這兩種病毒在其體內可能經互換基因而衍生新變種病毒，如果這新病毒會發生直接從人對人 (代替只從鳥-禽-畜對人)傳染，預期將會遇見全世界流行性感冒大流行開始。今年以來雖然因為在全球多國發生H1N1新型流感的泛流行疫情而轉移焦點，但仍應提高警覺，隨時注意高致病性禽流感H5N1的後續發展。

張伯俊 教授

 學歷：

國立陽明大學生物化學研究所博士

 經歷：

國立中興大學獸醫微生物學研究所長 (2006.09.01~)

國立中興大學獸醫微生物學研究所教授

美國Stanford大學遺傳研究所博士後研究

國立陽明大學微生物及免疫研究所博士後研究

發表期刊論文著作

· M.-S. Lee, et al., Genetic and Pathogenic Characterization of H6N1 Avian Influenza Viruses Isolated in Taiwan between 1972 and 2005. Avian Diseases 2006;

· C.-L. Chen, et al., Development of a Polymerase Chain Reaction Procedure for Detection and Differentiation of Duck and Goose Circovirus. Avian Diseases 2006; 50: 92-95

· C.-Y. Wang, et al., Expression of Capsid Proteins and Nonstructural Proteins of Waterfowl Parvoviruses in Escherichia coli and Their Application to Serological Assay. Avian Pathology 2005; 34: 1-7.

· H.-J. Tsai, et al., Genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives. Avian Diseases 2004; 48:512-521.

· H. K. Shieh, et al., Complete Nucleotide Sequences of S1 and N Genes of Infectious Bronchitis Virus Isolated in Japan and Taiwan. Jouranl of Veterinary Medical Science 2004. 66: 555-558

· J.-R. Wu, et al. 2003. Molecular characterization of plasmids with antimicrobial resistant genes in avian isolates of Pasteurella multocida. Avain Diseases. 47: 1384-1392 (SCI)

· C.-L. Chen, et al., 2003. Nucleotide sequences of goose circovirus isolated in Taiwan. Avian Pathology. 32, 165-171.

· J.-H. Shien, et al., 2002. Characterization of Newcastle disease virus isolated from Taiwan in 1998-2000. J. Chin. Vet. Sci. 28, 1-8.

· P.-C. Chang, et al., Expression of Infectious Laryngotracheitis Virus (ILTV) Glycoproteins in Escherichia coli and Their Application in Enzyme-linked Immunosorbent Assay. Avian Diseases 2002,46, 570-580.

O2 以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒

張伯俊1、謝快樂2、沈瑞鴻2

1國立中興大學微生物暨公共衛生學研究所, 2國立中興大學獸醫學系

中文摘要

本研究建立以multiplex RT-PCR配合RFLP技術，以快速鑑定及區分禽流感病毒(AIV)與新城病毒(NDV)，並可快速區分AIV之H5或H6，以及N1或N2亞型，另外也可區分NDV之疫苗株與田間強毒株。在AIV與 NDV之區分方面，以AIV之NP基因引子對配合NDV之F基因引子對進行multiplex RT-PCR，可得最佳檢測敏感度與特異性。在H5/H6亞型與N1/N2亞型之區分方面，以H5-310引子對配合H6-730引子對，以及N1-365引子對配合H6-639引子對，進行multiplex RT-PCR，可得最佳檢測敏感度與特異性。將RT-PCR產物以限制脢HaeIII及AluI切割進行RFLP分析，可確認RT-PCR之結果，並可區分美洲型(H5N2)與亞洲型(H5N1)毒株。本研究所建立之multiplex RT-PCR及 PCR/RFLP技術，可於5-6小時內診斷並確認H5N1、H5N2、H6N1及NDV感染，且不須經由定序之步驟（定序需1-2天），可大幅提昇AIV與NDV診斷之準確性與時效性。

Abstract

This study developed a multiplex RT-PCR and RFLP method for rapid identification and differentiation of avian influenza virus (AIV) and Newcastle disease virus (NDV). This method could also differentiate between H5, H6, N1 and N2 subtypes of AIV and differentiate vaccine strain from field strains of NDV. For identification of AIV and NDV, multiplex RT-PCR using primer pairs specific for the NP gene of AIV and for the F gene of NDV gave the best sensitivity and specificity. For differentiating between H5/H6 and N1/N2, multiplex RT-PCR using primer pairs H5-310/H6-730 and N1-365/H639 gave the best sensitivity and specificity. For RFLP analyses, the RT-PCR product amplified from the H5 gene was subjected to HaeIII and AluI digestion and the RFLP patterns obtained could differentiate H5 viruses (H5N2) of American lineage from those of Eurasian lineage (H5N1). In summary, the multiplex RT-PCR and RFLP method developed in this study could be used for rapid identification and differentiation of H5N1, H5N2, H6N1 virues and NDV in 5-6 hrs, and circumvent the need for sequence analyses.

劉宏仁 教授兼生技所所長

國立屏東科技大學 獸醫學系/ 生物科技研究所

分子病毒學暨生物技術研究室

學歷：

(1)美國奧本大學博士（1996年）:主修分子病毒學、生物技術及禽病學

(2)加拿大Manitoba大學醫學院內科及生理系博士後研究(1996-1997):

主持人經歷:

現職：國立屏東科技大學

生物科技研究所

教授兼所長

2006/08-迄今

國立屏東科技大學

獸醫學系

教授

2003/08-迄今

經歷: 南部科學園區

亞洲基因科技股份公司

董事會

獨立董事

2006/01-2009/06

財團法人生物技術開發中心

國立屏東科技大學

獸醫學系

專案顧問

副教授

2007/01-2008/12

2000/08- 2003/07

國立屏東科技大學

獸醫學系

助理教授

1997/08- 2000/07

加拿大Manitoba大學醫學院

內科及生理系

博士後研究員

1996/07- 1997/07

台灣省家畜衛生試驗所

豬瘟系

助理(高考及格分發)

1987/09-1991/08

研究成果目錄： (2007-2009)

1. Julius L. et al., Apoptosis induction by avian reovirus through P53 and mitochondrial pathways. BBRC 356: 529-535, 2007. (IF: 2.65) (SCI)

2 Hsin Y. et al., Apoptosis induction by avian reovirus related to tissue injury. Avian Pathology 36 :155-159, 2007. (IF: 1.7) (Vet. Sci. 18/134) (SCI)

3. Tang, D. G., et al., Display of avian influenza virus hemagglutinin on baculovirus envelope: effect of cytoplasmic domain on virus properties and potentials as vaccine. Molecular Therapy 15: 989-996, 2007. Corrigendium 15(9):1736-1736. (IF: 5.97) (SCI)

4. Hsiao-Ping Lee, et al., Variation of baculovirus-harbored transgene transcription among mesenchymal stem cell-derived progenitors leads to varied expression. Biotechnology & Bioenginering 97: 649-655, 2007. (IF: 2.94) (SCI)

5. Y. Y. Lien, et al., Cloning and nucleotide sequencing of the second internal transcribed spacer of ribosomal DNA for three species of Eimeria from chickens in Taiwan. The Veterinary Journal 173 : 186-191, 2007. (IF: 1.8) (Vet. Sci. 15/ 134) (SCI)

6.Yung Fu Wu, et al., Sequence and phylogenetic analysis of interleukin (IL)-1(-encoding genes of five avian species and structural and functional homology among these IL-1( proteins. Veterinary Immunology and Immunopathology 116:37-46, 2007. (IF: 1.91) (Vet. Sci. 12/134) (SCI)

7. Su, Y. P., et al., Avian reovirus core protein {micro}A expressed in Escherichia coli possesses both NTPase and RTPase activities. J. General Virology 88: 1797-1805, 2007. (IF: 3.05) (SCI)

8. Shen, P. C., et al., Genetic variation of the λA and λC protein encoding genes of avian reovirus. Research in Veterinary Science 83: 394-402, 2007. (IF: 1.38) (Vet. Sci. 34/134) (SCI)

9. Sue T. et al., Suppression of bovine ephemeral fever virus by RNA interference. Journal of Virological Methods 145: 84-87, 2007. (IF: 2.08) (SCI)

10. Chi Y. Wang , et al., The cleavage of the hemagglutinin protein of avian influenza virus in Pichia pastoris. J. Virological Methods 146:293-297, 2007. (IF: 2.08) (SCI).

11. Liu, H. J.* and Yao C. Hsieh. Control and prevention of avian influenza virus. Science Development 417: 62-69, 2007.

12. Julius L. C. et al., Avian reovirus p17 protein causes G2/M cell cycle arrest through inhibition of p34Cdc2. J. International Cooperation, 2: 12-25, 2007.

13. Wen T. et al., Supression of S-class genes of avian reovirus by RNA interference. Veterinary Microbiology 129: 252-261, 2008. (IF: 2.37) (Vet. Sci. 5/134) (SCI).

14. Chi-Young Wang, et al., Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of Newcastle disease virus. Veterinary Microbiology 130: 28-36. 2008. (IF: 2.37) (Vet. Sci. 5/134) (SCI).

15. Wu, Y. P., et al.,. Avian reovirus gene expression is required for the induction of chicken IL-1B mRNA expression in chicken peripheral blood mononuclear cells. J. General Virology 89:1059-1068, 2008. (IF: 3.05) (SCI)

16. Shu K. Li, et al., Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of avian reovirus. J. Virological Methods 49: 35-41, 2008. (IF: 2.08) (SCI).

17. Yu T. Chen, et al., Proteasome inhibition reduces avian reovirus replication and apoptosis induction in cultured cells. J.Virological Methods 151: 95-100, 2008. (IF: 2.08) (SCI).

18. Hung-Jen Liu . et al., Activation of small Gtpase Rho A and Rac 1 is required for avian reovirus p10-indiuced syncytiun formation. Molecule and Cells 26: 396-403, 2008. (IF: 1.92) (SCI)

19. Xin-Gang Xu, et al., Baculovirus surface display of Erns envelope glycoprotein of classical swine fever virus. J. Virological Methods 153:149-155, 2008 (IF:2.08) (SCI).

20. Ching-Ju Yeh, et al., TNFα mediates pseudorabies virus-induced apoptosis via theactivation of p38 MAPK and JNK/SAPK signaling. Virology 581:55-66, 2008. (IF 3.54) (SCI).

21. Review article: Wen T. Ji, Hung J. Liu*. PI3K-Akt signaling and viral infection. Recent Patent on Biotechnology 2 (3): 218-226, 2008. (SCI)

22. Xin-Gang Xu and Hung-Jen Liu*. Baculovirus surface display of E2 envelope glycoprotein of classical swine fever virus and immunogenicity of the displayed proteins in mouse model. Vaccine 26: 5455-5460, 2008. (IF: 3.3) (Vet. Sci. 1/134) (SCI).

23. Yueh H. et al., Baculovirus surface display of σC and σB proteins of avian reovirus and immunogenicity of the displayed proteins in a mouse model. Vaccine 26: 6361-6367 2008. (Vet. Sci. 1/134) (IF 3.3) (SCI).

24. Ping-Yuan Lin, et al., Modulation of p53 by mitogen-activated protein kinase pathways and protein kinase C delta during avian reovirus S1133-induced Apoptosis. Virology 385:323-324, 2009. (IF 3.54) (SCI)

25. Chi H. Lin, et al., Bovine ephemeral fever virus-induced apoptosis requires virus gene expression and activation of Fas and mitochondrial signaling pathway. Apoptosis 14: 864-877, 2009. (IF: 3.97) (SCI)

26. Wen T. Ji et al.,. Avian reovirus influences phosphorylation of several factors involved in

host protein translation including eukaryotic translation elongation factor 2 (eEF2) in Vero

cells. BBRC 384: 301-305, 2009. (IF: 2.65) (SCI)

27. Julius L. et al., Cell cycle regulation. Recent Patent on Biotech.3 (1): 1-9, 2009. (SCI)

28. Hung-Jen Liu , et al., Introduction of recombinant baculovirus of avian influenza. Veterinary Medical Association of Tainan County 14 : 96-117.

29. Xin-Gang Xu, et al., Baculovirus surface display of NS3 nonstructural protein of classical swine fever virus. J. Virological Methods 159:259-264, 2009. (IF: 2.08) (SCI).

30. Hung-Jen Liu. Introduction of immunoglobulin in yolk (IgY). CCPC Magazine 71: 28-33, 2009.

O3 禽流感之基因重組桿狀病毒疫苗之開發

劉宏仁 教授兼所長

國立屏東科技大學 生物科技研究所, 獸醫學系

Baculovirus display系統已成為研發次單位疫苗之重要工具。被表現之蛋白質可表現於桿狀病毒之封套，形成所謂 ”重組桿狀病毒”。此重組桿狀病毒可作為次單位疫苗。家禽流行性感冒病毒(avian influenza virus ; AIV)為正黏液病毒科(Orthomyxoviridae)之A型流行性感冒病毒。高病原性家禽流行性感冒(highly pathogenic avian influenza, HPAI)對雞或火雞造成高度傳染或急速死亡。Hemagglutinin (HA)和Neuraminidase (NA)為家禽流行性感冒病毒套膜上的主要免疫原。傳統的作法利用桿狀病毒系統所表現的目標蛋白存在於昆蟲細胞細胞質中。我们成功將H5N2禽流感HA及NA蛋白以鑲嵌的方式表現在桿狀病毒外套膜，以製備基因重組桿狀病毒。我们研究亦發現若以原本屬於桿狀病毒包膜蛋白gp64之胞質區段(cytoplasmic domain，CTD) 來取代HA蛋白本身之CTD可更有效率地將此HA蛋白鑲嵌並表現在桿狀病毒的外套膜上。我們以Baculovirus display表現平台構築了兩種禽流感之重組桿狀病毒(Bac-HA64 & BacSC-NA)，經由免疫螢光/共軛焦顯微鏡的觀察，兩種病毒在感染昆蟲細胞後，皆可以有效地表現重組HA及NA蛋白於細胞膜上。而由免疫金電子顯微鏡觀察，兩種重組蛋白也都可以展現在病毒的套膜上。為進一步探討研發之基因重組桿狀病毒是否適合開發成有效的次單位疫苗，因此進行雞隻疫苗效力及攻毒實驗免疫實驗，結果顯示雞隻經Bac-HA64重組桿狀病毒次單位疫苗免疫兩次後，HI抗体可達64-128倍以上並完全保護雞隻攻毒後免於致病。另HI抗体力價達16倍以上者之雞隻使用108.5 ELD50之H5N1病毒攻毒可得完全保護效果。這些結果顯示HA抗原適合開發成有效的次單位疫苗，用以防備禽流感。

蘇耀期 副教授

國立嘉義大學 農學院  獸醫學系

學　　歷：台灣大學獸醫學 博士

簡要經歷：

　　1977-1979年任職美國雅培藥廠動物部業物代表。

　　1981年公務人員高等考試暨專門職業技術人員高等考試獸醫類組及格。

　　1983-1986年任職南投縣政府家畜疾病防治所技士。

　　1986-1987年任職省農林廳畜牧科技士。

　　1985-1987年兼任國立嘉義農專獸醫科講師。

　　1987-1989年任職國立嘉義農專畜產科專任講師。

　　1989-1999年任國立嘉義農專及嘉義技術學院畜產系專副教授。

　　1991-1994年兼任國立嘉義農專進修部教務組組長。

　　1999年 國立嘉義大學農學院獸醫學系專任副教授迄今。

(A)期刊論文

1. Chiu, S.Y., et al., 2000. Studies of eyes of pursang cat(s): (II) Anisocornea, concomittent iris reaction, and unilateral iris response upon light illumination in relation to the distances of light source. A. Environ. Microbiol. (Vol. II) 8: N0.4 117-122.  

2. Su, Y.C., Feng, C.Y. 2000. Endoparasites of Ardeidae birds in Taiwan.  AEMl. (Vol. III) 9:11-18.

　3. Lin, P.H., Su, Y.C. 2000. The Effects of Native Chickens Inoculated Eimeria tenella and Dietary Diclazuril Supplementation on the Growth Performance and Immune Response. Jour. of the Biomass Energy Society of China.  18(3-4 ): 151-159.

　4. Su, Y.C., et al., 2000. Antibiotic sensitivity of pathogenic isolates: (V) Durgs having isolates with moderate sensitivity of gram nagative bacilli during 1995-1998. AEM. (Vol. II) 8:101-107.

　5. Mar, P.H., Su, Y.C. Fei, A.C.Y. Bowman, D.D. 2000. A survey of endoparasitic zoonoses of stray dogs and cats in Taipei city. Appl. Environ. Microbiol. Vol. II 8(3):77-86.

　6. Chiu, SY. et al., 2000. Legal infection disease : (IV) distribution of case numbers and chronological curves of Japanese B encephalitis of last decade in the republic of china. AEM. Vol. III 9( 1):3-9.

　7. Chiu, S.Y. et al., 2000.Studies of AIDS and HIV-seropositive infections in the republic of china: (III) Comparative analysis of AIDS/HIV-1 seropositive case numbers of various administrative areas of this country. Microbiol. Vol. III 9(2):35-47.

　8. Chiu, S.Y. et al., 2001. Interaction of Snail and Tropical Fishes In Aqua Environment L1) Study of Movements of Snail(s), The Speed, Dirrections Under Room Temperature. AEMl. 10: 9-15.

　9. Shiu SY, et al., 2002. Studies of body temperatures(1) Body temperatures of varies animals icluding human beings. A. Environ. Microbiol. 10: 65-73.

　10.. Chiu SY, et al., 2001. Interaction of snail and tropical fishes in Aqua Environment L1) Study of Movements of Snail(s), The Speed, Dirrections Under Room Temperature. AEM. 10: 9-15.

　11. Su YC, et al., 2003. Differential diagnosis of five avian Eimeria species by polymerase chain reaction using primers derived from the internal transcribed spacer1 (ITS-1) sequence. Vet. Parasitol. 117: 221-227. (SCI)

　12. Shiu SY, et al., 2004. Antibiotics with moderate percentages of sensitive isolates during 1998-2001. A. Environ. Microbiol. 12: 57~64.

　13. Su YC, Fei ACY. 2004. Endoparasites of the Crested Goshawk, Accipiter trivirgatus formosae, from Taiwan, Republic of China. Comparative Parasitology. 71(2), 178~183. (SCI)

14. 謝志宏等。2008。家禽種禽及孵化場之沙門氏桿菌疫情與抗藥性分析。嘉大農林學報5:70-86。

15. Chang-You Yu, et al., 2008. Comparison of the association of age with the infection of Salmonella and Salmonella enterica serovar Typhimurium in Pekin ducks and Roman geese. Poultry Science.

16. Maw-Rong Chao, et al., 2007. Assessing the Prevalence of Salmonella enterica in Poultry Hatcheries by Using Hatched Membranes. Poultry Science.86:1651-1655. (SCI, impact factor: 1.747)

O4 乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於

Methicillin與Vancomycin之抗藥性情形

蘇耀期、李研懷

國立嘉義大學獸醫學系

摘 要

葡萄球菌在人及動物為普遍造成食物中毒、傷口及醫源性的感染源，由於金黃色葡萄球菌具強烈致病性與對抗生素具有抗藥性，相關的研究也很多。金黃色葡萄球菌引起之牛羊乳房炎亦為一重要的問題，為瞭解乳羊場分離的葡萄球菌之抗藥性的分佈情形，首先將臨床分離菌株(2006~2008 分離之S. aureus113株及S. lentus 31株)進行16種抗生素敏感性試驗，將所獲得之結果進行分析後，發現普遍地存在著多重抗藥性，其中2006~2007分離之MRSA為0%(0/86)，VRSA為64.0%(55/86)，而2008年分離之MRSA則為44.4%(12/27)， VRSA為29.6%(8/27)；另外2008年分離之MRSL為100%(31/31)，VRSL為96.8%(30/31)；將此菌株再輔以最小抑菌濃度(MIC)試驗，將兩者結果進行分析與比較發現MRSA MIC50 為16， MIC90 為>256，VRSA MIC50 為 16~24，MIC90 為48，另MRSL MIC50和 MIC90 分別為>256，VRSL 的MIC50 和MIC90分別為32及64 均呈現抗藥性質。我們繼續探討乳羊與人源性MRSA的MecA與VacA,B,C,D基因檢測及脈衝電泳分析其同源關係。

張簡志炫 醫師

嘉義基督教醫院整形外科

學歷

中山醫學大學醫學系

國立嘉義大學微免所

經歷

高雄長庚醫院整形外科主治醫師中華民國整形外科專科醫師 中華民國美容外科醫學會會員

Publications

1. Chang-Chien CH. Bacteraemic necrotizing fasciitis with compartment syndrome caused by non-O1 Vibrio cholerae. J Plast Reconstr Aesthet Surg. 2006;59(12):1381-4.

2. Chang-CHien CH. et al., Bacteremic necrotizing facsciitis with compartment syndrome caused by non-O1 Vibro cholerae. JPRAS. 2006; 59:1381-1384.

3. Chang-Chien CH, Ding HT, Liu C, Yang CS. Vibrio infection associated with finning injury of the hand. Injury. 2007 May;38(5):614-8.

O5 Methicillin-resistant Staphylococcus aureus associated necrotizing fasciitis and mastitis

Chih-Hsuan Changchien1, 2, Wan-Lin Chen2, and Chishih Chu1

Department of Microbiology and Immunology, National Chiayi University1; Department of Plastic and Reconstructive Surgery, Chiayi Christian Hospital2

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most predominant microorganisms responsible for skin and soft tissue infection. MRSA have emerged as a significant pathogen in the microbiology of necrotizing fasciitis (NF), a fulminant soft tissue infection, and postpartum mastitis, a process of subcutaneous cellulitis. Our goals were to (1) determine the correlations of clinical, epidemiological, and microbiological features of MRSA with NF and mastitis, and (2) examine the choice of empiric antibiotic therapy.

Methods: Staphylococcal chromosomal cassette mec (SCCmec) types, pulse-field gel electrophoresis (PFGE) patterns, and virulence factors of 16 MRSA isolated from NF patients and 11 MRSA isolated from mastitis patients in 2008 were determined. Medical records were reviewed, including demographic characteristics, comorbid underlying conditions, and clinical features.

Results: Most MRSA NF infections were usually monomicrobial. Over 90% of MRSA isolates were resistant to erythromycin and clindamycin. Resistance to trimethoprim-sulfamethoxazole (SXT) significantly differed between HA-MRSA isolates and CA-MRSA isolates. Molecular analysis of 16 available MRSA isolates indicated that the SCCmec III and nontypeable type were only identified in HA-MRSA, whereas SCCmec IV and V were present in the 6 CA-MRSA isolates. All isolates possessed γ-hemolysin and only CA-MRSA isolates encoded Panton-Valentine leukocidin (PVL) genes. Virulence factors TSST-1, ETA, and ETB of MRSA were rarely detected among NF patients.

Of the mastitis-associated MRSA isolates, 10/11 (90.9%) was HA-MRSA (i.e., a history of hospitalization, surgery in the past year). However, antimicrobial resistant patterns and PCR results indicate that most isolates may be CA-MRSA. Mastitis caused by MRSA isolates was significantly less resistant to SXT (0), fucidin (0), clindamycin (72.7%), and erythromycin (72.7%) than those of other MRSA clinical syndromes. SCCmec IV and V types accounted for 81.9% of mastitis-associated MRSA. PVL genes were frequently identified. Mastitis had progressed to breast abscess and required surgical drainage in 1 MRSA infection, carrying PVL gene. None of the MRSA strains isolated from patients with mastitis carried the TSST-1 genes.

The SmaI-digested PFGE analysis of the 16 MRSA isolates associated with NF identified 8 PFGE patterns, 11 MRSA isolates associated with mastitis identified 6 PFGE patterns. Pulsotypes A, C, and D accounted for 68.8% of NF isolates and 72.7% of mastitis isolates.

Conclusions: MRSA is a significant emerging cause of NF and mastitis in Taiwan. In endemic areas, treatment of NF and mastitis patients should target MRSA with initial empiric antibiotics, exclusive erythromycin and clindamycin.

廖明輝 教授兼系主任 (Ming-Huei Liao)

屏東科技大學獸醫系 生物科技研究所

最高學歷：國立臺灣大學碩士

期刊論文：

1.

Chiu SY, et al., Appearance of arthropod Taiwanese prior to the 331 earthquack in Taipei city. A Environ Microbiol 11: 51-57, 2003.

2.

Chiu SY, et al., Investigations of merchandize, electric instruments, house safety, refrigerators and electronic calculators during the 331 earthquack in Taipei city. A Environ Microbiol 11: 59-70, 2003.

3.

Chiu SY, Liao MH, Su YC, Mar PH. Pseudofood without nutritional value found in readily eatable package or canes, drinks or tap water of this isolated island of west pacific ocean. A Environ Microbiol 11: 71-81, 2003.

4.

Liu HJ, et al., Detection of infectious bronchitis virus by multiple polymerase chain reaction and nucleotide sequence analysis. J Virol Methods 109: 31-37, 2003. (SCI)

5.

Chiu SY, et al., Chronological curves of some legal infectious diseases including hemorrhagic fever, rabies and plague of this country in the 20th century. A Environ Microbiol 12: 1-8, 2003.

6.

Chiu SY, et al., Minimal pupil size and rotation of eye ball of Siamese cat. A Environ Microbiol 12: 15-19, 2003.

7.

Yin CC, et al., Identification of Ehrlichia canis in cats by nested polymerase chain reaction and nucleotide sequence analysis. Taiwan Vet J 29: 122-128, 2003.

8.

Chiu SY, et al., Comparative analysisof changing of pupil shape upon light shinning on siamese cat(s) and pursingcat(s). A Environ Microbiol 12: 23-29, 2003.

9.

Chiu SY, et al., Some plants grown in the garden with indoor decorational value without roots. A Environ Microbiol 12: 37-41, 2003.

10.

Liu HJ, et al., Molecular evolution of avian reovirus: evidence for genetic diversity and reassortment of the S-class genome segments and multiple cocirculating lineages. Virology 314: 336-349, 2003. (SCI)

11.

Chiu SY, et al., The external structures in some detail of a moth found in a high building. A Environ Microbiol 12: 51-55, 2003.

12.

Chiu SY, et al., Antibiotics with moderate percentages of sensitive isolates during 1998-2001. A Environ Microbiol 12: 57-64, 2003.

13.

Yu FL, et al., Induction of hepatoma cells migration by phosphoglucose isomerase/autocrine motility factor through the upregulation of matrix metalloproteinase-3. Biochem Biophy Res Comm 314: 76-82, 2004. (SCI)

14.

Chang CJ, et al., Apoptosis induced by bovine ephemeral fever virus. J Virol Methods 122: 165-170, 2004. (SCI)

15.

Yu FL, et al., Hepatitis B virus X protein promotes cell migration by inducing matrix metalloproteinase-3. J Hepatol 42: 520-527, 2005. (SCI)

O6 犬隻沙氏桿菌分子流行病學之調查

1劉紋紋 2陳玄峰 1盧明志 1廖明輝

1國立屏東科技大學獸醫學系 2國立屏東科技大學生物科技研究所

摘要

沙氏桿菌為革蘭氏陰性菌，是人畜共通傳染病，造成人類嚴重的胃腸炎、菌血症及傷寒等疾病與經濟損失。其感染途徑，主要是吃到污染病原的食物，感染後病原亦可從糞便排出；但某些無臨床症狀的感染動物成為潛在性的帶菌者，由糞便中排出沙氏桿菌而污染環境造成動物及人類的感染。

隨著生活型態的改變，圈養寵物的人口逐年攀升，由家庭寵物來源感染的沙氏桿菌症成為新的關注焦點；本調查係針對南部地區隨機採檢犬隻糞便，並參照世界動物衛生組織（International Office of Epizootics；OIE）分離步驟，進行沙氏桿菌的分離鑑定。共採集2,000件糞便檢體進行鑑定，共分離出295隻沙氏桿菌，其中自動物醫院分離出的陽性株64件（21.7%；64/295）、寵物店21件（7.1%；21/295），動物收容中心210（71.2%；210/295）件；分離率以動物收容中心為最高。

進一步以脈衝式膠體電泳（pulsed-field gel electrophoresis, PFGE）基因圖譜與傳統血清型試驗鑑定分析沙氏桿菌分離株，共鑑定出12種血清型，其中以S. Albany（75%）及S. Newport（13.2%）居多；藥物敏感性試驗中顯示，藥物抗藥性比例以tetracycline（86.8%）、ampicillin（78.5%）及sulfamethoxazole- trimethoprim（75.2%）三種藥物最高；從上述結果顯示與人們一起生活的寵物，有可能成為人類沙氏桿菌症的潛在感染來源，藉由以上資料，可提供民眾於飼養寵物的衛教資訊及臨床獸醫師對犬隻施用抗生素之參考依據，並提供未來進行沙氏桿菌感染之風險評估資料之依據。

關鍵詞：沙氏桿菌、脈衝式膠體電泳、藥物敏感性試驗

金 立 德副教授

國立嘉義大學微生物與免疫學系

學歷

瑞 典Lund 大學 免疫技術學所 博 士

國防醫學院 微生物免疫學所 碩 士

輔仁大學 生物學系 學 士

經歷

源一生物科技 執行長

五鼎生物技術 研發部經理

普生公司 研發處處長

輔仁大學 生物學系副教授

成功大學 醫事技術學系助理教授

中華血液基金會 研究處處長

國防醫學院 預醫所助理研究員

A.期刊論文

1. Wu, S.-Y., et al., (2008) Direct visualization of fluorescent signals in protein gels using a backlit blue light plate. Proteomics 8:3382-3388.

2. Chin, L.-T., et al., (2008) Immune intervention with monoclonal antibody targeting to CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J 31:1-15.

3. Meng, C.-L., et al., (2007) Preventing and Ameliorating 7,12-Dimethylbenz[a]anthracene (DMBA)-induced DNA adducts formation by garlic in cultured human endometrial cells. J Biomed Lab Sci 19:131-139.

4. Chin, L.-T., et al., (2007) Site-directed in vitro immunization leads to a complete human monoclonal IgG4λ that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands. BMC Biotechnology 7:51.

5. Leu, I., et al., (2004) Evaluation of a Device for Self-Monitoring of Blood Glucose. J Biomed Lab Sci 16:57-66.

6. Chin, L.-T., et al., (2001) Establishment and evaluation of mouse-human heteromyeloma cell lines obtained by electrofusion for immortalizing human immunoglobulins. J Biomed Lab Sci 13:117-123.

7. Lu, S.-C., et al., (2001) Seroprevalence and demographic characteristics of HTLV-I among blood donors in Taiwan: 1996-1999. Int J Hematol 74:333-7.

8. Lu, S.-C., et al., (2001) Intrafamilial transmission and risk assessment of HTLV-I among blood donors in southern Taiwan. Kaohsiung J Med Sci 17:126-32.

B. 專利

1. 金立德、許淑菁 可攜式經皮傳導裝置，中華民國M308755

2. 金立德、許淑菁 製造具有生物受體協同劑、拮抗劑與/或反向協同劑性質之人

類抗體製造方法 中華民國I 267517

3. 傅貽平、許淑菁、李棟銘、金立德 由冷凍血漿產製血纖維原、原血纖維酵素複體、第三抗凝血酵素、免疫球蛋白與白蛋白之流程 中華民國 I 226333

4. 金立德、許淑菁、傅貽平 由冷凍血漿產製病毒去活化之免疫球蛋白與白蛋白之流程 中華民國577924

5. 沈家琳、劉宜中、林岳暉、黃英哲、沈承禹、金立德、沈燕士 具有樣品前處理功能之電化學感測器 中華民國5 73122

6. 金立德、林岳暉、沈承禹、沈燕士 預存裝置 中華民國 I 182892

7. 金立德 體外免疫誘製人類單株型抗體方法及應用此法所誘製之抗人類免疫缺陷病毒之中和抗體 中華民國I 062901

8. Li-Te Chin; Shu-Ching Hsu, Method for producing human antibodies to human CD152 with properties of agonist, antagonist, or inverse agonist. 美國7,494,77

9. 金立德、許淑菁 製造具有生物受體協同劑、拮抗劑與/或反向協同劑性質之人類抗體製造方法中國ZL200410079889.3

10. Li-Te Chin; Shu-Ching Hsu, Zusammensetzung und Reagenz für CTLA-4 und Verwendungen derselbe, 德國Nr. 20 2008 000 834.4

11. 金立德、許淑菁。可攜式經皮傳導電子裝置。中國ZL 2006 2 0118081.6.

12. Li-Te Chin; Shu-Ching Hsu, Tragbare transdermale Vorrichtung. 德國Nr.20 2007 001 908.

13. Li-Te Chin; Shu-Ching Hsu, Nieuwe uit memselijke kiemlijnsequenties gewonnen antilichaamstructuren. 荷蘭1029605.

14. Li-Te Chin; Shu-Ching Hsu; Chishih Chu, Aus humanen keimbahnsequenzen gewonnene antikörperstrukturen. 德國Nr. 20 2005 020 828.0.

O7 High mobility group 1 (HMGB1) protein as the therapeutic target for severe sepsis and septic shock

Li-Te Chin

Department of Microbiology and Immunology, National Chiayi University

Severe sepsis and septic shock represent the major cause of the overall mortality among infected patients. The pathogenesis of sepsis is mediated in part by bacterial endotoxin like lipopolysaccharide (LPS), which stimulates macrophages/monocytes to sequentially release early (e.g., TNF-α, IL-1β) and late pro-inflammatory cytokines. Recently, it has been found that levels of high mobility group 1 (HMGB1) protein remain high in the majority of patients suffering severe sepsis and septic shock, indicating that the cytokine indeed is a downstream and late mediator of inflammation and thus sepsis. HMGB1 is actually a nuclear factor and simultaneous a secreted protein. It was originally identified as an architectural chromatin-binding factor that bends DNA, promotes protein assembly on specific DNA targets and thus facilitating chromosome construction in the nucleus. After secretion from activated monocytes and macrophages, it binds with high affinity to RAGE (the receptor for advanced glycation end products) and TLR (Toll-like receptors), becoming a potent mediator of inflammation extracellularly. In humans, serum HMGB1 concentrations were elevated in adult patients with severe sepsis and septic shock compared with healthy controls. Furthermore, anti-HMGB1 antibodies were capable of rescuing mice from lethal experimental sepsis, even when the first doses are given 24 h after the onset of sepsis, indicating a wider window for HMGB1-targeted therapeutic strategies. Therefore, agents capable of attenuating released HMGB1 may have potential in the prevention and treatment of inflammatory diseases. As a platform technology for induction of complete human monoclonal antibodies has been established, additional studies and collaboration are required to fully define the relationship of HMGB1 to therapeutics of septic disease.

嚴重的敗血症及敗血性休克是目前感染病患中最主要的死因，敗血症之致病機轉部分肇因於如脂多醣的細菌性內毒素，其會刺激巨噬細胞或單核球狀、使其繼而釋出如TNF-α與IL-1β等早期細胞激素及晚期發炎反應性細胞激素。近年來之研究指出第一高游動性組蛋白質(high mobility group 1; HMGB1)在絕大多數嚴重敗血症及敗血性休克患者體內濃度皆有升高，暗示了HMGB1即為位於下游負責敗血症發生的晚期發炎反應性細胞激素。HMGB1因此事實上不但是一種細胞核因子、也同時是種分泌性蛋白質，HMGB1最早是因其具有染色質結合和扭曲DNA之能力而被鑑別出來，在細胞核中促使蛋白質在特定的DNA上組合的結構蛋白，故與染色體的組裝有關。一旦當它被活化的單核細胞或巨噬細胞釋放，則會與RAGE (the receptor for advanced glycation end products)以及 TLR (Toll-like receptors)等受體以高親合力結合、在細胞外也能因此成為強力的發炎反應媒介物。嚴重的敗血症及敗血性休克病患其血清HMGB1濃度遠超過健康正常人，此外在動物試驗中HMGB1抗體甚至還能於實驗動物發展出敗血症徵候24小時後再施打第一劑來拯救動物、使其不致發生致死性敗血症；這些結果給HMGB1作為治療標的開了一扇窗，因此能夠中和釋出HMGB1的藥物對預防及治療全身性的發炎疾病將極有潛力；由於吾人已建立了完全人類單株抗體之載台技術。因此，進一步的研究與合作對於成功地治療敗血病相當重要。

余章游教授

國立嘉義大學農學院  獸醫學系

學　　歷：中興大學獸醫學博士

　　　　　臺灣大學獸醫學學士

簡要經歷：

　　嘉義農專助教、嘉義農專講師、嘉義技術學院講師、嘉義大學副教授

　

(A)期刊論文

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

11.

12.

13. 余章游、張文興、王俊秀。2006。提升Leucocytozoon caulleryi及荒川庫蠔(Culicoides arakawae)培養效率。台灣獸醫誌32:129-135。陳綵慈、蔡秀敏、余章游、翁博群、陳國隆。2006。飼糧中添加β-聚葡萄糖對來航小公雞抵抗盲腸型球蟲病及免疫力之影響。中畜會誌 35:101-108。

14. Maw-Rong Chao et al., 2007. Assessing the prevalence of Salmonella enterica in poultry hatcheries using hatched eggshell membranes. Poultry Science 86:1651-1655. (Impact factor: 1.747; 15.9% top, 7/44)

15. 吳錫勳、 余章游、陳綵慈、葉瑞涵、蔡銘洋、陳國隆。2007。紐西蘭白兔與雷克斯兔於懷孕及哺乳期間血液生化成分之比較。中畜會誌36(2)：93-101。

16. Chang-You Yu et al., 2008. Prevalence and Characterization of Multidrug-Resistant (Type ACSSuT) Salmonella enterica serovar Typhimurium Strains in Isolates from Four Gosling Farms and a Hatchery Farm. Journal of Clinical Microbiology 46:522-526. (Impact factor: 3.445; 23.9% top, 21/89)

17. Shih-Jen Chou, Yao-Ching Shieh, Chang-You Yu. 2008. Changes of Hematological and Biochemical Values during Botulism Afflicted and Recovered Periods in Black-faced Spoonbills (Platalea minor). Journal of Wildlife Diseases. (SCI, impact factor: 0.987; 32.56% top, 42/129; in press)

18. 謝志宏、朱紀實、蘇耀期、王誠明、余章游。2008。家禽種禽及孵化場之沙門氏桿菌疫情與抗藥性分析。嘉大農林學報5:70-86。

19. Chang-You Yu et al., 2008. Genomic diversity and molecular differentiation of Riemerella anatipestifer. Avian Pathology. (SCI, impact factor: 1.809; 9.3% top, 12/129; accepted)

20. Chang-You Yu et al., 2008. Comparison of the association of age with the infection of Salmonella and Salmonella enterica serovar Typhimurium in Pekin ducks and Roman geese. Poultry Science. (SCI, impact factor: 1.747; 15.9% top, 7/44; accepted)

21. Chang-You Yu et al., 2008. The valuation of blood and tissue enzymes for clinical diagnosis in New Zealand White and Rex rabbits. Taiwan Vet. J. (revised)

O8 嘉義地區沙門氏菌在水禽幼雛及蛋膜感染

Salmonella infection in young birds and hatched egg membranes in waterfowls in Chiayi area

Chang-You Yu,*§ Jiang-Liang Lin,* Chishih. Chu,* Yao-Chi Su,§ Shu-Wun Chen, * Pei-Chun Tu,

§Department of Veterinary Medicine, *Department of Microbiology and Immunology, National Chiayi University

ABSTRACT Salmonella enterica causes a number of significant poultry diseases and is also a major pathogen in humans. Most poultry infected by Salmonella become carriers; infection may also be fatal, depending on the particular serovar and the age of the bird at infection. Younger birds are more susceptible to infection by Salmonella, so it is critical that hatcheries monitor birds. We used cloacal swabs and hatched eggshell membranes (HEMs) to evaluate Salmonella prevalence in waterfowl hatcheries and young geese. Six serogroups (A, B, C1, C2, D, and E) of Salmonella were detected in the hatcheries. S. Typhimurium was dominant in one goose hatchery and also in the geese from this hatchery that had been transferred to a farm. However, Salmonella serogroup C1 was the major serogroup found in goose and duck hatcheries. Salmonella Potsdam and Montevideo belonged to C1 were the major serotypes in hatcheries and young goslings. Genetic study by plasmid and PFGE analysis showed that isolates from duck hatchery were more diverse than those from goose. In S. Potsdam, isolates with genotype 3, 4 and 5 were isolated from goose hatcheries; in contrast, isolates with genotype 7, 8 and 9 were only identified in duck’s. We suggest that S. Potsdam may evolve into genotypic isolates to infect goose and duck separately.

O9 The change of Salmonella genomic island and R plasmid-mediating survival in THP-1 cells for Salmonella enterica serovar Virchow

Yung-Lu Lee1, Chien-Shun Chiou2, Chishih Chu1,

1Graduate Institute and Department of Microbiology and Immunology, National Chiayi University, 2The Central Branch Office, Center for Disease Control

Salmonella genomic island (SGI1) is a 43-kb DNA mobile element region found firstly in S. Typhimurium and consist of genes blaPSE, floR, aadA2, sulI, and tetG responsible for resistance to ACSSuT resistance. Complex class 1 integron In104 of most SGI1 types is inserted between res (or tnpR) and ORF44. However, In104-related InSGI1-J is located within ORF S023. Previously, we reported SGI1-J in human S. Virchow isolates from 1994. We investigated the evolution of SGI1-J and genetic variations of 98 S. Virchow human isolates from 2004 to 2006 by PCR amplification and PFGE as well as plasmid analysis. Antimicrobial resistance was associated mostly with the deletions in the InSGI1-J. Genetic adaptation of S. Virchow adaptation occurs in reduction of PFGE types and increase of plasmid complexity. Further, the deletion results in dramatic change in InSGI1-J. Two ampicillin resistance plasmids of two strains confirmed the resistance to bacteriocidal effect of THP-1 cells.

李永茹

國立陽明大學 微免所博士班學生

國立嘉義大學 微免所碩士

朱紀實(Chishih Chu), Ph.D.

Associate Professor , Department of Microbiology and Immunology, National Chiayi University

學歷

Michigan State University, Plant Breeding and Genetics – Forestry, PhD

National Taiwan University, Graduate Institute of Forestry, MS

National Chung Hsing University, Deaprtment of Forestry, Bachelor

經歷

Company/ University

Title

Genetic Engineering Center

NationalChung-Hsing University

Postdoctor

General Biologicals Corp

Associate Researcher

Apex Biotechnology Corp.

Advisor researcher

Humorigin Biotechnology Corp

Director of Scientific

Research

Publications (Recent 3 years)

1.

Chiu CH, et al., 2007. Variant Salmonella genomic island 1 antibiotic resistance gene clusters in Salmonella enterica serovar Derby isolates from humans in Taiwan. J Antimicrob Chemother. 59(2):325-6. [SCI, Impact factor = 4.328, 30/216 in PHARMACOLOGY & PHARMACY ]

2.

Lee CH, et al., 2007. Collateral damage of flomoxef therapy: in vivo development of porin deficiency and acquisition of blaDHA-1 leading to ertapenem resistance in a clinical isolate of Klebsiella pneumoniae producing CTX-M-3 and SHV-5 {beta}-lactamases. J Antimicrob Chemother. 60(2):410-3. [SCI, Impact factor = 4.328, 30/205 in PHARMACOLOGY & PHARMACY ]

4.

Lin CL, et al., 2007. A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi. J Microbiol Immunol Infect. 40:222-6.

5.

Chen KL, et al., 2007. Effect of caponization and testosterone implantation on hepatic lipids and lipogenic enzymes in male chickens. Poult Sci. 86(8):1754-9. [SCI, Impact factor = 1.668, 8/45 in AGRICULTURE, DAIRY & ANIMAL SCIENCE]

6.

Chin LT, et al.,2007. Site-directed in vitro immunization leads to a complete human monoclonal IgG4lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands. BMC Biotechnol. 7:51 [SCI, Impact factor = 2.747, 41/138 in BIOTECHNOLOGY & APPLIED MICROBIOLOGY]

7.

Chao MR, et al., 2007. Assessing the prevalence of Salmonella enterica in poultry hatcheries by using hatched eggshell membranes. Poult Sci. 86:1651-5. [SCI, Impact factor = 1.668, 8/45 in AGRICULTURE, DAIRY & ANIMAL SCIENCE]

8.

Huang KY, et al., 2007. Molecular epidemiology and antimicrobial susceptibility of Salmonella enterica serotype Stanley isolates in Taiwan. J Microbiol Immunol Infect. 40:411-8

9.

Yu CY, et al., 2008. Prevalence and Characterization of Multi-Drug Resistant (ACSSuT) Salmonella enterica serovar Typhimurium isolated from four gosling’s farms and a hatchery farm. J Clinic Microbiology 46(2):522-6. [SCI, Impact factor = 3.945, 18/91 in MICROBIOLOGY ]

10.

Yu CY, et al., 2008. The genome diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms. Avian Pathology 37:273-279. [ SCI, Impact factor = 1.700, 18/134 in VETERINARY SCIENCES]

11.

Su LH, et al., 2008. An epidemic of plasmids? Dissemination of extended-spectrum cephalosporinases among Salmonella and other Enterobacteriaceae. FEMS Immunol Med Microbiol. 52(2):155-68. [SCI, Impact factor = 1.928, 86/119 in IMMUNOLOGY]

12.

Chin LT, et al., 2008. Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant disease. Chang Gung Med J. 30:1-15.

13.

Hong SF, et al., 2008. Complete nucleotide sequence of a virulence plasmid of Salmonella enterica serovar Dublin and its phylogenetic relationship to the virulence plasmids of serovars Choleraesuis, Enteritidis and Typhimurium. FEMS Microbiology Letters 282:39-43. [SCI, Impact factor = 2.021, 52/91 in MICROBIOLOGY]

14.

Chen, RS., et al., 2008. Differentiation of two powdery mildews of sunflower (Helianthus annuus L.) by PCR-mediated method based on ITS sequences. Eur. J. Plant Pathol. 121:1–8. [SCI, Impact factor = 1.482, 59/152 in PLANT SCIENCES]

15.

Yu CY, et al., 2008.Comparison of the association of age with the infection of Salmonella and Salmonella enterica serovar Typhimurium in Pekin ducks and Roman geese. Poult Sci. 87:1544-9. [SCI, Impact factor = 1.668, 8/45 in AGRICULTURE, DAIRY & ANIMAL SCIENCE]

16.

Scaria J., et al., 2008. Microarray for molecular typing of Salmonella enterica serovars. Molecular & Cellular Probes. 2008 22(4):238-43. [SCI, Impact factor = 2.364, 26/60 in BIOCHEMICAL RESEARCH METHODS.]

17.

Chu C, et al., 2008. Evolution of genes on the Salmonella Virulence plasmid phylogeny revealed from sequencing of the virulence plasmids of S. enterica serotype Dublin and comparative analysis. Genomics. 92: 339-343. [SCI impact factor = 3.075 35/144 in BIOTECHNOLOGY & APPLIED MICROBIOLOGY]

18.

Chen KL, et al., 2008. (-1,3-1,6-glucan indirectly enhances the phagocytic and bactericidal capability of peritoneal macrophage in chicks to Salmonella enterica serovar Enteritidis. Poult. Sci. 87: 2242-2249. [SCI, Impact factor = 1.668, 8/45 in AGRICULTURE, DAIRY & ANIMAL SCIENCE]

19.

Doublet B, et al., 2009. Truncated tni module adjacent to the complex integron of salmonella genomic island 1 in Salmonella enterica serovar Virchow. Antimicrob Agents Chemother. 53:824-7. [SCI, IF=4.716, 21/216 in PHARMACOLOGY & PHARMACY]

20.

Feng Y, et al., 2009. Apoptosis-like cell death induced by Salmonella in Acanthamoeba rhysodes. Genomics. 2009 May 12. [Epub ahead of print]

O10 Immunity of Chicken to Salmonella enterica serovar Enteritidis infection

Chishih Chu1, Kuo-Lung Chen2, Chang-You Yu3

Department of Microbiology and Immunology1, Department of Animal Sciences2, Department of Veterinary Medicines3, National Chiayi University

S. Enteritidis is major pathogen to cause outbreak of foodborne salmonellosis through contaminated poultry eggs, meats and their products. The amounts and functions of peritoneal macrophages were investigated. Maximal number of peritoneal macrophages was found at age of 31-33 days. There were no difference in phagocytic ability for peritoneal macrophages collected from 18-42-days-old chicks, However, intracellular bacteria were completely removed for peritoneal macrophages from 29-days-old chicks. To enhance of immunity of chicks younger than 29-day-old, (-1,3-1,6-glucan of Schizophyllum commune and peritoneal macrophages from 22-day-old chicks were used. Oral feeding of 0.1% of (-1,3-1,6-glucan was enough to protect the S. Enteritidis infection in liver and spleen. The protected mechanism of (-1,3-1,6-glucan was directly interaction with peritoneal macrophages to enhance phagocytic and bactericidal effect. Further, we investigated the route of S. Enteritidis infection on eggs. 2-year-old female chickens were challenged with S. Enteritidis by the following methods: cloacal, oral, intraperitoneal (IP) and intravenous (IV) injection. Salmonella contamination was examined in egg membrane, albumen, yolk membrane, and yolk. Salmonella was found on eggs treated with all methods. However, IP injection showed maximal infection in all egg parts. Major infection was found on egg membrane, suggesting that Salmonella infects mainly oviduct. Although highest antibody titer to S. Enteritidis was found in chicks treated by IP method, Salmonella was still found in eggs of IP groups. It implicate that antibody might protect not enough for S. Enteritidis infection through macrophage transmission.

葉光勝副教授

臺北醫學大學 醫學系微生物與免疫學科

學歷

美國愛荷華大學 醫學院微生物學系 Ph.D. 1997

美國奧本大學 獸醫學院病理生物學系 M.S. 1992

國立中興大學 獸醫學系 B.S. 1970/ 09 至1974/06

經歷

台北醫學大學 醫學系微生物免疫學科 助理教授 2004-2008

國立中興大學 獸醫病理研究所 兼任助理教授 2001 / 08-2004/07

台灣動物科技研究所 動物醫學組 副研究員 1977 / 10-2003/08

　

國立臺灣大學 醫學院生理學科 研究助理 1989/ 06-1990/06

　

期刊論文 (2005-2009)

1. Hsuan SL, et al., 2009. Efficacy of a novel Pasteurella multocida vaccine against progressive atrophic rhinitis of swine. Vaccine. 27(22):2923-9.

2. Lin J, et al., 2009. Staphylococcus aureus isolated from pork and chicken carcasses in Taiwan: prevalence and antimicrobial susceptibility. J Food Prot. 72:608-11.

3. Lin CC, et al., 2009. Analysis of ciprofloxacin-resistant Salmonella strains from swine, chicken, and their carcasses in Taiwan and detection of parC resistance mutations by a mismatch amplification mutation assay PCR. J Food Prot. 72:14-20.

4. Chien MS, et al., 2009. Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages.Vet Microbiol. 135:327-33.

5. Chuang YC, et al., 2008. Primers specific for the fimbrial major subunit gene stdA can be used to detect Salmonella enterica serovars. J Food Prot. 2008 Jun;71(6):1108-13.

6. Chuang Y-C, et al., 2008. Identification of the genetic determinants of Salmonella enterica serotype Typhimurium that may regulate the expression of the type 1 fimbriae in response to solid agar and static broth culture conditions. BMC Microbiol.; 8: 126.

1.

7. Su, J-J, et al., 2006. A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan. Current Microbiology 53: 77-81 (SCI).

　

8..

Lin, J.-H., et al., 2006. Mycoplasma hyorhinis in Taiwan: diagnosis and isolation of swine pneumonia pathogen. Veterinary Microbiology 115: 111-116 (SCI).

　

9.

Chen, T.-C., et al., 2006. Serotype occurrence and antimicrobial susceptibility of Salmonella isolates recovered from pork carcass in Taiwan (2000 through 2003). Journal of Food Protection 69: 674-678 (SCI).

　

10.

Wang, Y.-C., et al., 2006. Fluoroquinolone-resistant Salmonella sp. in carcasses. Emerging Infectious Diseases 12 (2): 351-352 (SCI).

　

11.

Lo, D.-Y., et al., 2006. Effects of sugar cane extract on Pseudorabies virus challenge of pigs. Journal of Veterinary Medical Science 68: 219-225 (SCI).

　

12..

C.-C. Chang, et al., 2005. Epidemiologic relationship between fluoroquinolone- resistant Salmonella enterica serovar Choleraesuis strains isolated from humans and pigs in Taiwan (1997-2002). Journal of Clinical Microbiology 43: 2798-2804 (SCI).

　

13..

Yeh, K.-S., et al., 2005. One-year (2003) nationwide pork carcass microbiological baseline data survey in Taiwan. Journal of Food Protection 68 (3): 458-461 (SCI).

O11 A constitutively mannose-sensitive agglutinating Salmonella enterica serovar Typhimurium LB5010 strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes

1吳冠勳, 2王克銓, 3許元勳, 3葉光勝

1台北市立萬芳醫院小兒科, 2臺北醫學大學醫學科學研究所,

3臺北醫學大學醫學系微免學科

Salmonella enterica serotype Typhimurium produces a variety of fimbrial appendages, among which the type 1 fimbriae is the most common type. In vitro, static broth culture favours S. Typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. Previous study indicated that a transposon inserted in the stbC gene, which would encode an usher protein for Stb fimbriae of a non-flagellar S. Typhimurium LB5010 strain, conferred it to agglutinate yeast cells on both static broth and solid agar media constitutively, and was mannose-sensitive. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that the expression of fimA, a major fimbrial subunit gene for type 1 fimbriae, and fimZ, a positive regulator gene of fimA, were increased in the stbC mutant strain more than in the parental strain when grown on LB agar; fimW, a repressor gene of fimA, exhibited lower expression. Nonetheless, fimbrial appendages were not observed on the stbC mutant with electron microscopy; instead, flagella structures were expressed. Flagella formation was correlated with the motile phenotype detected by MSRV agar medium and reaction with the flagellar antiserum. Microarray data and RT-PCR also indicated that the expression of three genes involved in chemotaxis/flagella mechanism, motA, motB, and cheM, was enhanced in the stbC mutant. In addition, the S. Typhimurium stbC mutant was resistant to a variety of antibiotics, consistent with the finding that expression of yhcQ and ramA, two genes involved in multidrug resistance, was enhanced. A complementation test revealed that transforming a recombinant plasmid possessing the coding sequence of stbC gene restored a stbC mutant to exhibit the mannose-sensitive agglutination phenotypes as the parental S. Typhimurium LB5010 strain, however, the flagellar formation and multidrug resistance phenotypes were not affected in the complemented strain. The present study indicates that a transposon inserted in the stbC gene of S. Typhimurium enables it to exhibit mannose-sensitive agglutination, flagellar structures, and resist to a variety of antibiotics. Complementing the stbC strain with the coding sequence of stbC restores the mannose-sensitive agglutination phenotypes as the parental strain, indicating the possibility of interplay of different fimbrial system to coordinate their expression.

Key word: Salmonella typhimurium, type 1 fimbriae, stbC, multidrug resistance, flagella

邱政洵教授 (Dr. Cheng-Hsun Chiu)

Office Address: Department of Pediatrics, Chang Gung Children's Hospital,

5, Fu-Shin St., Kweishan 333, Taoyuan, Taiwan, R.O.C.

Education:

1. 1.M.D. June, 1989, Chung Shan Medical and Dental College

2. Ph.D. September, 1997, Graduate Institute of Clinical Medicine, Chang Gung University College of Medicine (Supervisor: Prof. Jonathan T. Ou)

3. Postdoctoral Fellow, October 1997-March 1999, Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada (Supervisor: Prof. David P. Speert)

Employment Record:

1. Resident, June 28, 1989-June 30, 1992, Department of Pediatrics, Chang Gung Memorial Hospital.

2. Chief Resident, July 1, 1992-June 30, 1993, Department of Pediatrics, Chang Gung Memorial Hospital.

3. Attending Physician, since July 1, 1993, Department of Pediatrics, Chang Gung Children's Hospital.

4. Assistant Professor, July 1, 1995-June 30, 2000, Department of Pediatrics, Chang Gung Children's Hospital and Chang Gung University.

5. Associate Professor, since July 1, 2003, Department of Pediatrics, Chang Gung Children's Hospital and Chang Gung University.

6. Professor, since July 1, 2005, Department of Pediatrics, Chang Gung Children's Hospital and Chang Gung University.

Publications:

1. Chen CJ, et al., Seroprevalence of human papillomavirus types 16 and 18 in the general population in Taiwan: implications for optimal age of human papillomavirus vaccination. J Clin Virol 2007;38:126-30.

2. Tang P, Chiu CH, and Lee YS coauthored with other 62 authors. Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis. Science 2007;315:207-12.

3. Liu SY, et al., Characterization of plasmids encoding CTX-M-3 extended-spectrum (-lactamase from Enterobacteriaceae isolated at a university hospital in Taiwan. Int J Antimicrob Agents 2007;29:440-5.

4. Wu TL, et al., CMY-2 (-lactamase-carrying community-acquired urinary tract Escherichia coli: genetic correlation with Salmonella enterica serotypes Choleraesuis and Typhimurium. Int J Antimicrob Agents 2007;29:410-6.

5. Chiu CH, et al., Variant Salmonella genomic island 1 antibiotic resistance gene clusters in Salmonella enterica serovar Derby isolates from humans in Taiwan. J Antimicrob Chemother 2007;59:325-6.

6. Lin TY, et al., One-year post-primary antibody persistence and booster immune response to a fully liquid five-component acellular pertussis, diphtheria, tetanus, inactivated poliomyelitis, Haemophilus influenzae type b conjugate vaccine. Int J Infect Dis 2007; 11:488-95.

7. Chen SY, et al., Molecular epidemiology and clinical manifestations of viral gastroenteritis in hospitalized pediatric patients in northern Taiwan. J Clin Microbiol 2007; 45: 2054-7.

8. Chang YJ, et al., Fulminant Clostridium septicum infection mimicking appendicitis in a healthy child. Ann Trop Paediatr 2007;27:91-4.

9. Lin JJ, et al., Bullous impetigo: a rare presentation in fulminant streptococcal toxic shock syndrome. Pediatr Emerg Care 2007;23:318-20.

10. Chiu CH, et al., Increasing ceftriaxone resistance and multiple alterations of penicillin-binding proteins among penicillin-resistant Streptococcus pneumoniae in Taiwan. Antimicrob Agents Chemother 2007; 51:3404-6.

11. Lin CL, et al., A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi. J Microbiol Immunol Infect 2007;40:222-6.

12. Chen CJ, et al., Clinical features and molecular characteristics of invasive community-acquired methicillin-resistant Staphylococcus aureus infections in Taiwanese children. Diagn Microbiol Infect Dis 2007;59:287-93.

13. Lin CH, et al., A cluster of adenovirus serotype 3 infections in children in northern Taiwan: clinical features and laboratory findings. J Microbiol Immunol Infect 2007;40:302-9.

14. Su LH, Chiu CH (corresponding author). Salmonella: clinical importance and evolution of nomenclature. Chang Gung Med J 2007;30:210-9.

15. Tsai MH, et al., Nontyphoidal Salmonella bacteremia in previously healthy children: analysis of 199 episodes. Pediatr Infect Dis J 2007;26:909-13.

16. Huang KY, et al., Molecular epidemiology and antimicrobial susceptibility of Salmonella enterica serotype Stanley isolates in Taiwan. J Microbiol Immunol Infect 2007;40:411-8.

17. Chen CJ, et al., Experience with linezolid therapy in children with osteoarticular infections. Pediatr Infect Dis J 2007;26:985-8.

18. Ho CF, et al., Clinical analysis of computed tomography-staged orbital cellulitis in children. J Microbiol Immunol Infect 2007; 40:518-24.

19. Lee PI, et al., Taiwan Pediatric Working Group on the Management of CAP in Children. Guidelines for the management of community-acquired pneumonia in children. Acta Paed Taiwan 2007;48:167-80.

20. Feng Y, et al., Evolution and pathogenesis of Staphylococcus aureus: lessons learned from genotyping and comparative genomics. FEMS Microbiol Rev 2008;32:23-37.

21. Yu CY, et al., Prevalence and characterization of multidrug-resistant (type ACSSuT) Salmonella enterica serovar Typhimurium strains in isolates from four gosling farms and a hatchery farm. J Clin Microbiol 2008;46:522-6.

22. Su LH, et al., An epidemic of plasmids? Dissemination of extended-spectrum cephalosporinases among Salmonella and other Enterobacteriaceae. FEMS Immunol Med Microbiol. 2008;52:155-68.

23. Hong SF, et al., Complete nucleotide sequence of a virulence plasmid of Salmonella enterica serovar Dublin and its phylogenetic relationship to the virulence plasmids of serovars Choleraesuis, Enteritidis and Typhimurium. FEMS Microbiol Lett 2008;282:39-43.

24. Lin TY, et al., Booster vaccination at 6-8 years of age with a reduced antigen content dTpa-IPV vaccine is immunogenic and safe after priming with whole-cell pertussis vaccine. Hum Vaccin 2008; 4:50-3.

25. Chao HC, et al., The impact of constipation on growth in children. Pediatr Res 2008; 64:308-11.

26. Kuo CY, et al., Antimicrobial susceptibility of Shigella isolates in eight Asian countries, 2001-2004. J Microbiol Immunol Infect 2008; 41:107-11.

27. Chuang CH, et al., Surveillance of antimicrobial resistance of Salmonella enterica serotype Typhi in seven Asian countries. Epidemiol Infect 2008; 12:1-4.

28. Yu CY, et al., Genomic diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms. Avian Pathol 2008; 37:273-9.

29. Chao HC, et al., Cutoff volume of dietary fiber to ameliorate constipation in children. J Pediatr 2008; 153:45-9.

30. Huang KY, et al., Enterovirus 71 in Taiwan, 2004-2006: epidemiological and virological features. Scand J Infect Dis 2008; 40:571-4.

31. Nyambat B, et al., Survey of childhood empyema in Asia: implications for detecting the unmeasured burden of culture-negative bacterial disease. BMC Infect Dis 2008; 8:90.

32. Jaing TH, et al., Successful unrelated cord blood transplantation in a girl with malignant infantile osteopetrosis. Chinese Med J 2008; 121:1245-6.

33. Tsai MH, et al., Pneumococcal meningitis in Taiwanese children: emphasis on clinical outcomes and prognostic factors. J Trop Pediatr 2008; 54:390-4.

34. Chu C, et al., Evolution of genes on the Salmonella virulence plasmid phylogeny revealed from sequencing of the virulence plasmid of S. enterica serotype Dublin and comparative analysis. Genomics 2008; 92:339-43.

35. Cheng CH, et al., Renal abscess in children: a 10-year clinical and radiologic experience in a tertiary medical center. Pediatr Infect Dis J. 2008; 27:1025-7.

36. Tsai MH, et al., Seroprevalence of SARS coronavirus among residents near a hospital with a nosocomial outbreak. J Formos Med Assoc 2008; 107:885-91.

37. Cheng CH, et al., Antibiotic resistance patterns of community-acquired urinary tract infections in children with vesicoureteral reflux receiving prophylactic antibiotic therapy. Pediatrics 2008; 122:1212-7.

38. Lee HY, et al., Genotyping and antimicrobial susceptibility of Salmonella enterica serotype Panama isolated in Taiwan.J Microbiol Immunol Infect 2008;41:507-12.

39. Lee WI, et al., Chinese patients with defective IL-12/23-interferon-gamma circuit in Taiwan: partial dominant interferon-gamma receptor 1 mutation presenting as cutaneous granuloma and IL-12 receptor beta1 mutation as pneumatocele. J Clin Immunol 2009; 29:238-45.

40. Doublet B, et al., A truncated tni module adjacent to the complex integron of SGI1 in Salmonella enterica serovar Virchow. Antimicrob Agents Chemother 2009; 53:824-7.

41. Chen SY, et al., Acute gastroenteritis caused by multiple enteric pathogens in children. Epidemiol Infect 2009;137:932-35.

42. Chen CC, et al., Effect of probiotics L. acidophilus on C. rodentium colitis: the role of dendritic cells. Pediatr Res 2009;65:169-75.

43. Huang KY, et al., Prolonged fecal shedding of CTX-M-15-producing Escherichia coli and recurrent sepsis in a patient after cord blood stem-cell transplantation. Scand J Infect Dis 2009;41:224-7.

44. Chen CJ, et al., Host and microbiologic factors associated with mortality in Taiwanese children with invasive pneumococcal diseases, 2001 to 2006. Diagn Microbiol Infect Dis 2009; 63:194-200.

45. Lin JJ, et al., Community-acquired Pseudomonas meningitis causes acute obstructive hydrocephalus. Childs Nerv Syst 2009;25:723-5.

46. Chen SY, et al., Norovirus infection as a cause of diarrhea-associated benign infantile seizures. Clin Infect Dis 2009;48:849-55.

47. Lin YT, et al., Tigecycline and colistin susceptibility of Chryseobacterium meningosepticum isolated from blood in Taiwan. Int J Antimicrob Agents 2009;34:100-1.

48. Chen CC, et al., Effect of probiotics Lactobacillus acidophilus on Citrobacter rodentium colitis: the role of dendritic cells. Pediatr Res 2009;65:169-75.

49. Chen SY, et al., Two peculiar issues on pediatric enteric infections in Taiwan: Pseudomonas aeruginosa and polymicrobial infections. Pedictr Neonatol 2009;50:1-2.

50. Yang WC, et al., Salmonella septic arthritis involving multiple joints in a girl with acute lymphoblastic leukemia at diagnosis. Pediatr Neonatol 2009;50:33-5.

51. Lee HY, et al., High rate of reduced susceptibility to ciprofloxacin and ceftriaxone among nontyphoid Salmonella clinical isolates in Asia. Antimicrob Agents Chemother 2009; 53:2696-9.