Designer : 徐安涛、徐滔、钊超

张慧慧、吴瑕、吴珍

A 19-year-old woman presents for the evaluation of pelvic pain. The pain has progressively worsened over the past week. She has also sexually active, has had 4 lifetime partners, takes oral contraceptive pills, and occasionally uses condoms. On examination, she appears in no acute distress and does not have a fever. Her abdomen is soft with moderate lower abdominal tenderness. On pelvic examination, she is noted to have a yellow cervical discharge and significant cervical motion tenderness. No uterine or adnexal masses are palpated, but mild tenderness is also noted.

A Gram stain of the cervical discharge reveals only multiple polymorphonuclear leukocytes. A direct DNA probe test subsequently comes back positive for Chlamydia trachomatis.

Inclusion body

Rabies virus （ Negri body ）

Cytomegalovirus

•

• TE8

• TE55

•

1 、 filterable 120—200nm

2 、 obligate intracellular parasite

3 、 a unique life cycle

Elementary body （ EB) ：extracellular ； nondividing 300nm infectious particle;

stain –gymsa blue ,macchiavello red

Initial body (reticulate body ，RB): intracellular ;0.5-1.2µm , binary fission , non-infectious ， gymsa and macchiavello blue , no densible nuclear substance ,but has reticular structure

Join the bacteria in 1973

1 、 contain DNA and RNA

2 、 cell wall G- ， similar to G- bacteria LPS ： contain genus specific epitopeMOMP: Species specific & Type specific epitopes encoding gene and sequence 、 vaccine HSP60Species : C.trachomatis C. pneumoniae C. psittaci C.pecorum subspecies :Biovar trachoma LGV Biovar mouseTypes :C T (A-K, 14) LGV(L1,L2a,L2b L3;4)

No peptidoglycan but sensible to penicillin

Resistance :sensible to heat 60 5-10mi℃n,-70 several years; antibiotics ℃

Endotoxoid substance

MOMP : avoid the fusion of lysosome and vacuole of elementary body ; HSP60

Inflammation :

necrosis

exudation

proliferation

Acute inflammation : weeping,viscous supp

urative secretion , conjunctival conjestion

Chronic inflammation : scarring , entropion trichiasis etc.

1)Trachoma （ A 、 B 、 Ba 、 C ）

2)urogenital-tract infection, It is the main causative agent of nongonococcal urethritis(NGU)

In men , urethritis ,epididymitis ,

In women , urethritis ,cervicitis , pelvic inflammatory disease (PID)

3)Inclusion Conjunctivitis newborn,adult

4)Lymphogranuloma Venereum(LGV)

Main invade lymph tissue A painless papule on the genitali

a that heals

spontaneously

Designer : 徐安涛、徐滔、钊超

张慧慧、吴瑕、吴珍

Polymorphonuclear leukocyte

Extracelluar gram-negative diplococci

Intracelluar gram-negative diplococci

EB

Heparan sulfate ;Major outer membrane protein (MOMP)

RBBinary fission

Phagosome

AAs &ATP etc

Exocytosis

16—24 per ER

Primary differentiation

EB→RB

Secondary differentiation

RB→EB