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Cortical plaques
Lambotin et al, J Cell Science 2005
EzrinActine
(nid d’abeille)
Lambotin et al, J Cell Science 2005
Doulet et al, J Cell Biol 2006
Nécessaire à l’adhésion "forte" en condition de flux(G. Mikaty, G. Dumenil & coll)
PI3K
GTPases
β-arrestins
Lan Ma and Gang Pei, Journal of Cell Science 120, 213-218 (2007)
•MapK•Src•Akt
A
B C
β-arrestins - SiRNA
βarrestin 1/2
Actin
Con
trol
siR
NA
βar
rest
in 1
/2 s
iRN
A
2,5 fold
Exp
ress
ion
is
redu
ced
by
Beta arrestine 2GFP ezrin
Ezrin recruitment
0%
20%
40%
60%
80%
100%
ControlsiRNA
Arrestin 1+2sirRNA
FLNA sirRNA GRK2 sirRNA
Ezrin
β-arrestins - SiRNA
βarrestin 1/2
Filamin A
GRK2
Actin
Con
trol
siR
NA
βar
rest
in 1
/2 s
iRN
A
Fila
min
A s
iRN
A
GR
K2
siR
NA
2,5 fold
Exp
ress
ion
is
redu
ced
by
7,5 fold
3 fold
Beta arrestine 2GFP ezrin
Ezrin recruitment
0%
20%
40%
60%
80%
100%
ControlsiRNA
Arrestin 1+2sirRNA
FLNA sirRNA GRK2 sirRNA
Ezrin
Recepteur adrénergique
Beta2 AR - YFP ezrin
0
20
40
60
80
100
% o
f re
cru
itin
g c
olo
ny
Ezrin recruitment
Control Phentolamine10 uM
Propranolol10 uM
αα--antagonistantagonist ββ--antagonistantagonist
Recepteur adrénergique
Beta2 AR - YFP ezrin
+
ββ--agonistagonist
0
20
40
60
80
100
Ct 0,0001 0,01 1 10
Ezrin recruitment
% o
f re
cru
itin
g c
olo
ny
Isoproterenol (μM)
Recepteur adrénergique
Beta2 AR - YFP ezrin
ββ--agonistagonist
0
20
40
60
80
100
Ct 0,0001 0,01 1 10
Ezrin recruitment
% o
f re
cru
itin
g c
olo
ny
Isoproterenol (μM)
+
Recepteur adrénergique
Ezrin recruitment
0%
20%
40%
60%
80%
100%
cont
rol
Isopr
oter
enol
1uM
Isopr
oter
enol
100n
M
SDF1M
IP IL8
AT2
5 à10 x Kd
Ezrin recruitment
0%
20%
40%
60%
80%
100%
IsoproterenolPropranolol
- 10µM 10µM 1µM 1µM - - + - +
Signaling des protéines G
0
20
40
60
80
100
Control PKA-PS
20uM 10uM
Forkolin10-5 M
PKA-PS Forkolin10-6 M
Ezrin recruitment
% o
f re
cru
itin
g c
olo
ny
Activation partielle
Billes coatées, cellules hCMEC/D3
Phenylephrineα1 agoniste
Isoproterenolβ1 agoniste
contraste actin ezrin
BSA ou Metoprolol
Effet des agonistes adrénergiques
Nm - + - + P-Ezrin
P-Moesin
CtIsoproterenol
0
1
2
3
4
5
Ezr
in p
hosp
hory
latio
n fo
ld in
crea
se
CtIsoproterenol
0
20
40
60
80
100
Ct 0,0001 0,01 1 10
Recrutment d’Ezrin%
re
cru
itin
g c
olo
nie
s
Isoproterenol (μM)
Ct 0,0001 0,01 1 100 Iso +
Phe
10:10
0
20
40
60
80
100
% r
ecr
uiti
ng
co
lon
ies
Recrutment d’Ezrin
Phenylephrine (μM)