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KUMAR ORGANIC PRODUCTS LIMITED Ingredients for us BIO/002/2012-13 Dr. Lakshmi Sridhar 18 th Sep 2012

Cosmetic sodium hyaluronate

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Page 1: Cosmetic   sodium hyaluronate

KUMAR ORGANIC PRODUCTS LIMITED

Ingredients for us

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Page 2: Cosmetic   sodium hyaluronate

SODIUM HYALURONATE

(KOPURON)

Cosmetic grade

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Page 3: Cosmetic   sodium hyaluronate

Sodium Hyaluronate - Identification

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

• INCI name: Sodium hyaluronate

• CAS No. 9057-32-7

• Product name: Poly-(sodium-b-D-glucuronate-[1-3}- N-acetyl-D-glucosamine-[1-4]

•Other names: Hyaluronic acid, Hyaluron, Hyaluronan

• EINECS/ELNICS: 232-678-0

Page 4: Cosmetic   sodium hyaluronate

Sodium Hyaluronate - Structure

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Sodium hyaluronate –Is a linear chain muco-polysaccharide composed of disaccharide units formed from D-glucuronic acid & N acetylglucosamine held together by 1,3 linkages

Page 5: Cosmetic   sodium hyaluronate

• Fermentation: Streptococcus equi, subsp.

•Bacterial strain, non-haemolytic

• Non-GMO

•No animal products used during the manufacturing process

Source

BIO/001/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Page 6: Cosmetic   sodium hyaluronate

Process Flow Diagram

BIO/001/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Page 7: Cosmetic   sodium hyaluronate

Sodium Hyaluronate - specification

S.No. Tests Specification

1. Appearance White powder or granules

2.Transparency of 0.1% aqueous

solution

NLT 99.5%

3. Loss on drying NMT 20%

4. Residue on Ignition NMT 20%

5. pH (0.1% aqueous solution) 5.0 – 8.0

6. Protein content NMT 0.1%

7. Glucuronic acid 44 – 50 %

8. Sodium Hyaluronate 93 – 103 %

9. Molecular weight 1.0 – 1.6 MDa

10. Total bacterial count NMT 100 cfu/g

BIO/001/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Page 8: Cosmetic   sodium hyaluronate

Sodium hyaluronate in the body

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

• Hyaluronic acid – significant constituent of human skin, connective tissue, lubricant in joints, vitreous humor of the eye

Page 9: Cosmetic   sodium hyaluronate

Sodium Hyaluronate

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

• Skin has 10 to 20 % water• As we age, water is lost & the skin appears dry & wrinkled• Great ability to hold water – 1000 times its weight• Forms a film on the surface of skin to moisturize • Smoothens the skin – anti-wrinkle effects

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15 30 60 120 180 240 300 360

Hyd

rati

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ease

%

Time (mins)

0.025% Kopuron Max: Skin Moisturizing Effect

Page 10: Cosmetic   sodium hyaluronate

Sodium Hyaluronate

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Comparison between Sodium hyaluronate & other moisturizers under identical conditions

0 5 10 15 20 25 30 35 40

Hydration Increase %

Sodium pyrrolidonecyboxylate

Glycerine

Sorbitol

Kopuron Max

Page 11: Cosmetic   sodium hyaluronate

Sodium Hyaluronate

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

• Moisturizing property used in cosmetics for sun care, skin care, repair, lipsticks, pre & after shave, cleansers, hair care, etc.

Page 12: Cosmetic   sodium hyaluronate

Sodium hyaluronate

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Transparency – of 1 % aqueous solution of Sodium hyaluronate was measured at 660 nm – transparency of 99.99 % was measured – very transparent – ideal for cosmetic applications

0

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400 nm

600 nm

Ab

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Page 13: Cosmetic   sodium hyaluronate

Sodium Hyaluronate

Heat Stability – Aqueous solution of Sodium hyaluronate was incubated at different temperatures for different times. Corresponding changes in kinematic viscosity were measured. Sodium hyaluronate is stable at temperatures lower than 80 C

0

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0 15 30 45 60 75 90

Kin

em

ati

c V

isc

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Time (mins)

Kopuron Max: Heat Stability

60 deg C

80 deg C

90 deg C

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Page 14: Cosmetic   sodium hyaluronate

Sodium Hyaluronate

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

• Sodium hyaluronate’s moisturizing property demonstrated in lip sticks using corneum hydration measurements. Hydration increases with Kopuron Max

Page 15: Cosmetic   sodium hyaluronate

Sodium Hyaluronate

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

• Sodium hyaluronate in lipsticks decreases trans epidermal water loss (TEWL). Kopuron reduces TEWL.

Page 16: Cosmetic   sodium hyaluronate

SODIUM HYALURONATE

BIO/002/2012-13Dr. Lakshmi Sridhar

18th Sep 2012

Biodegradable & eco-friendly

Sodium hyaluronate is completely natural

Produced by bacterial fermentation

“Green technology”