Curriculum Vitae Jean-Denis Docquier · 2013-06-05 · Curriculum Vitae – Jean-Denis Docquier pagina 3 di 31 Institut di Biologie Structurale "Jean-Pierre Ebel" (Pr. Otto Dideberg),

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Curriculum Vitae

Jean-Denis Docquier

Dati personali

Data e luogo di nascita 15 gennaio 1974 - Liegi, Belgio

Cittadinanza Belga

Stato civile Celibe

Domicilio 15, Via De Gasperi, I-53018, Volte Basse - Sovicille (SI)

Telefono 349 6148419

Formazione

2002 Dottorato di Ricerca in Scienze (Ph.D. Biochemical Sciences), Università di

Liegi, Belgio.

Titolo conseguito il 19 novembre 2002, dopo discussione pubblica di una tesi

intitolata "Metallo--lactamases, from clinical aspects to post-genomics: a

genetic and biochemical study".

Tesi eseguita sotto la direzione scientifica dei Prof. Jean-Marie Frère

(Università di Liegi, Belgio) e Prof. Gian Maria Rossolini (Università degli

Studi di Siena).

Dottorato finanziato dall’Unione Europea (programma “Training and

Mobility of Researchers”) nell’ambito del “European Network on the Study of

Metallo-β-lactamases: Integrated Approach to the Catalytic Mechanism and

Synthesis of Inhibitors of Metallo-β-lactamases” (contratto n° ERB-FMRX-

CT98-0232).

1998 Laurea (M. Sc.) in Scienze Biochimiche, Università di Liegi, Belgio.

1995 Diploma ("Candidat") in Scienze Chimiche, Università di Liegi, Belgio.

Curriculum Vitae – Jean-Denis Docquier

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Attività scientifica e competenze

Incarico attuale

Ricercatore confermato, Facoltà di Farmacia (fino a ottobre 2012) e Dipartimento di

Biotecnologie Mediche (da novembre 2012), Area Scientifica Microbiologica, Settore

scientifico disciplinare “Microbiologia e microbiologia clinica” (MED/07) (dal 1 marzo 2008).

Attività scientifica svolta presso il Laboratorio di Fisiologia e Biotecnologie dei Microrganismi

(Direttore, Prof. G. M. Rossolini), Dipartimento di Biotecnologie Mediche.

Linee di ricerca

Aspetti clinici della resistenza batterica agli antibiotici ed epidemiologia molecolare.

Caratterizzazione funzionale e strutturale delle β-lattamasi, in particolare la

caratterizzazione molecolare delle metallo-β-lattamasi, come effettori di resistenza agli

antibiotici β-lattamici in batteri patogeni, e come possibile bersaglio di inibitori

enzimatici.

Studio di metallo-β-lattamasi e di proteine strutturalmente correlate, individuate per via

post-genomica, come paradigma dell’evoluzione molecolare.

Indirizzo di lavoro

Laboratorio di Fisiologia e Biotecnologia dei Microrganismi,

Dipartimento di Biotecnologie Mediche,

Università degli Studi di Siena,

Policlinico “Le Scotte”, II piano, V lotto,

Viale Bracci,

I-53100 Siena

Telefono: +39 0577 233134 (int. 3134)

Fax: +39 9577 233870 (int. 3870)

E-mail: [email protected]

Altri laboratori frequentati

Centre d’Ingénierie des Protéines & Laboratoire d’Enzymologie (Pr. Jean-Marie Frère),

Università di Liegi, Liegi, Belgio.


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Institut di Biologie Structurale "Jean-Pierre Ebel" (Pr. Otto Dideberg), CNRS-CEA,

Grenoble, Francia.

Competenze

Tecniche di biologia molecolare, inclusa espressione eterologa.

Microbiologia di base, molecolare ed applicata.

Purificazione, analisi e caratterizzazione funzionale e strutturale delle proteine.

Lingue

Francese (lingua madre), inglese e italiano.

Attività di referaggio per riviste scientifiche

Membro dell’ “Editorial Board” (2009-2011) di Antimicrobial Agents and Chemotherapy

(ASM Press, Washington DC, USA)

Membro dell’ “Editorial Advisory Board” di Biochemical Pharmacology (Elsevier,

Oxford, UK)

Membro dell’ “Editorial Board” di The European Journal of Microbiology and

Immunology (Akademiai Kiado, Budapest, Hungary)

Membro dell’ “Editorial Board” di ISRN Microbiology (Hindawi Publishing

Corporation, New York, U.S.A.)

Journal of Antimicrobial Chemotherapy (Oxford University Press, UK)

Biochemistry (American Chemical Society, Washingtion DC, USA)

Microbiology (Society for General Microbiology, Reading, UK)

Clinical Microbiology and Infection (Blackwell Publishing, Oxford, UK)

Diagnostic Microbiology and Infectious Diseases (Elsevier, Amsterdam, The

Netherlands)

Journal of Infection (Elsevier, Amsterdam, The Netherlands)

Journal of Basic Microbiology (Wiley-VCH, Weinheim, Germany)

Journal of Medical Microbiology (Society for General Microbiology, Reading, UK)

Chemotherapy (Karger AG, Basel, Switzerland)

BMC Structural Biology (BioMed Central Ltd, London, UK)

PLOS ONE

Chemistry and Biology (Cell Press, Cambridge, USA)

Accounts of Chemical Research (American Chemical Society, Washingtion DC, USA)


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ACS Medicinal Chemistry Letters (American Chemical Society, Washingtion DC, USA)

Miscellaneo

Membro della “American Society for Microbiology” (ASM).

Membro della “Alliance for the Prudent Use of Antibiotics” (APUA).

Membro della “European Society of Clinical Microbiology and Infectious Diseases”

(ESCMID).

Membro del “ESCMID Study Group for Antimicrobial Resistance Surveillance”

(ESGARS).

Membro della Società Italiana di Microbiolgia (SIM).

Membro della Società Italiana di Microbiologia Medica, Odontoiatrica e Clinica

(SIMMOC)


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Attività didattica

Università degli Studi di Siena

2002 Professore a contratto della Facoltà di Farmacia, Insegnamento di

Microbiologia (Prof.ssa G. Coratza), modulo “Laboratorio di Microbiologia”

(MED/07), Laurea Specialistica in Chimica e Tecnologie Farmaceutiche (20

ore).

2002-2006 Collaboratore alla didattica, Microbiologia Applicata (Prof.ssa G. Coratza),

Corso Integrato di Biotecnologie Cellulari e Molecolari, Laurea Specialistica

in Biotecnologie per la Salute Umana, Facoltà di Farmacia (20 ore).

2003 Collaboratore alla didattica, Insegnamento di Microbiologia (Prof. G. M.

Rossolini), modulo “Microbiologia II”, Laurea Specialistica in Chimica e

Tecnologia Farmaceutiche, Facoltà di Farmacia (20 ore).

2004-2007 Professore a contratto della Facoltà di Farmacia, Insegnamento di

Microbiologia, modulo II “Laboratorio di Microbiologia” (MED/07), Laurea

Specialistica in Chimica e Tecnologia Farmaceutiche (24 ore).

dal 2006 Professore a Contratto della Facoltà di Medicina e Chirurgia, Insegnamento

“Proteine – DNA”, area propedeutica, Scuola di Specializzazione in

Microbiologia e Virologia (15 ore).

2007-2008 Professore a contratto della Facoltà di Farmacia, Insegnamento di

Microbiologia Applicata, Corso Integrato di Biotecnologie Cellulari e

Molecolari, Laurea Specialistica in Biotecnologie per la Salute Umana,

Facoltà di Farmacia (24 ore).

2008 Insegnamento di Microbiologia, modulo I “Microbiologia” e modulo II

“Laboratorio di Microbiologia” (MED/07), Laurea Specialistica in Chimica e

Tecnologia Farmaceutiche (44 ore).

2008 Insegnamento di Microbiologia Applicata, Corso Integrato di Biotecnologie

Cellulari e Molecolari, Laurea Specialistica in Biotecnologie per la Salute

Umana, Facoltà di Farmacia (24 ore).

2008 Insegnamento (corso a scelta studente) di Microbiologia Farmaceutica e

Applicata, Laurea Specialistica in Chimica e Tecnologia Farmaceutiche,

Facoltà di Farmacia (24 ore).


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2009-2011 Insegnamento di “Molecular Microbiology”, Modulo I “Molecular

microbiology for drug discovery (formulation and delivery)” e modulo II

“Vaccines Biotechnology”, Laurea Magistrale in “Pharmaceutical

Biotechonology” (in lingua inglese) (60 ore).

2010-2012 Insegnamento di “Analysis of Biotechnological Drugs”, Modulo II “Applied

and Pharmaceutical Microbiology”, Laurea Magistrale in “Pharmaceutical

Biotechonology” (in lingua inglese) (30 ore).

dal 2010 Insegnamento di “Analisi Microbiologiche”, Scuola di Specializzazione in

Farmacia Ospedaliera (8 ore).

dal 2010 Insegnamento di “Microbiologia ed Elementi di Epidemiologia”, Laurea

Magistrale in Farmacia (72 ore).

dal 2010 Insegnamento di Patologia Generale e Terminologia Medica e Microbiologia”

- Modulo II “Microbiologia”, Laurea Magistrale in Chimica e Tecnologia

Framnaceutiche (60 ore).

Altre università o istituzioni

1998 Collaboratore alla didattica, Laboratorio di Enzimologia (30 ore), Laurea in

Scienze Biochimiche, Facoltà di Scienze, Università di Liegi, Belgio.

2002 “Tutorial session, Detection of metallo--lactamases: kinetic aspects and

ancillary tests” (2 ore), 18th ESCMID Postgraduate Education Course

"Diagnostics, Characterization and Epidemiology of -lactamases", 22-23

aprile, 2002, Orta San Giulio, Italia.

2004 Collaboratore alla didattica, Laboratorio di Enzimologia (30 ore), Laurea in

Scienze Biochimiche, Facoltà di Scienze, Università di Liegi, Belgio.

2004 “Post-Graduate Course in Biochemistry, Topics in Biochemistry: Bacterial

Resistance to Antibiotics” (4 ore), Scuola di Dottorato in Scienze

Biochimiche, Università di Liegi, Belgio.

2004 Seminario di aggiornamento, “Problemi emergenti di antibiotico-resistenza

in ambiente ospedaliero e comunitario” (3 ore), Scuola di Specializzazione in

Microbiologia e Virologia, 28 maggio, 2004, Università degli Studi di Pavia.

2004 “Genomics, Proteomics and Resistance” (1 ora), Master in Microbiologia,

Università di Parigi V, VI and VII, Francia.


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2006 “36th ESCMID Post-Graduate Educational Course, “Detection and

Characterisation of Metallo-β-Lactamases”, 26-28 maggio 2006, Verona,

Italia

2008 “Post-Graduate Course in Biochemistry, Topics in Biochemistry: Bacterial

Resistance to Antibiotics” (12 ore), Scuola di Dottorato in Scienze

Biochimiche, Università di Liegi, Belgio.

Dal 2009 “Bacterial Resistance” (6.5 ore), Master in Biochimica e Biologia Moleculare

e Cellulare (corso opzionale), Università di Liegi, Belgio.


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Borse di studio, riconoscimenti e finanziamenti

Borsa di Dottorato dell’Unione Europea (Training and Mobility of Researchers

Program, 1998-2001) within the "European Network on the Study of Metallo--

Lactamases: Integrated approach to the catalytic mechanism and synthesis of inhibitors of

metallo--lactamases" (contract n° ERB-FMRX-CT98-0232).

“Collaborateur Scientifique et Chargé de Recherches”, Fonds National de la Recherche

Scientifique, Belgio (ottobre 2003 – settembre 2007).

Assegnista di Ricerca, Università di Siena (gennaio 2003 – dicembre 2006)

Student and Post Doctoral Travel Grant, American Society for Microbiology. 43rd

Interscience Conference on Antimicrobial Agents and Chemotherapy, September 14-17,

2003, Chicago, U.S.A. Comunicazioni premiate: (1) Biochemical characterization of

IMP-13, a new IMP variant produced by a Pseudomomas aeruginosa clinical isolate

from Italy, abstract n° C1-660. (2) Functional heterogeneity of VIM-type metallo--

lactamases: identification of residues critical to substrate binding, abstract n° C1-

662.

Coautore di un riassunto premiato dal “Programme Committee Award”, 44th

Interscience Conference on Antimicrobial Agents and Chemotherapy, 30 ottobre - 2

novembre, 2004, Washington, U.S.A. (Iannelli F., Santagati M., Docquier J.D.,

Cassone M., Oggioni M.R., Rossolini G.M., Stefani S., Pozzi G. Type M resistance

to macrolides in Streptococci is not due to the mefA gene, but to matA encoding an

ATP-dependent efflux pump, abstract n° C1-1188).

ESCMID Research Fellowship 2004, European Society of Clinical Microbiology and

Infectious Diseases (Award Committee President, Dr. Roger Finch).

ICAAC Young Investigator Award 2005, Interscience Conference on Antimicrobial

Agents and Chemotherapy, American Society for Microbiology.

ECCMID 2006 Travel Grant, European Society of Clinical Microbiology and Infectious

Diseases.

Premio “Agathon De Potter 2006” per la Microbiologia e la Biologia Vegetale, Académie

Royale des Sciences, Lettres et Beaux-Arts de Belgique.

Finanziamento “Grandi Attrezzature” – Fondazione Monte dei Paschi di Siena (importo

155.000 €), 2008.

Finanziamento “Regione Toscana” - Programma per la Ricerca Regionale in Materia di

Salute 2009 (importo 391.650 €), 2010 (Responsabile Unità di Ricerca).


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Attività istituzionale

Membro eletto del Consiglio del Dipartimento di Biologia Molecolare, Università di

Siena (rappresentante degli assegnisti di ricerca dal 2003 al 2006).


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Partecipazione a seminari, corsi di formazione e

congressi nazionali o internazionali

1. 2nd International Meeting on the Therapy of Infections, 18-21 novembre, 1998, Firenze,

Italia.

2. European Union Metallo--Lactamase Network Annual Meeting, 12-14 marzo, 1999,

Saarbrücken, Germania.

3. International EU/ESF Practical Training Course on "Chemistry of Metals in Biological

Systems", 15-25 maggio, 1999, Louvain-la-Neuve, Belgio.

4. International Congress on -Lactamases. "-lactamase Mediated Resistance: Molecular

Aspects and Clinical Implications", 17-20 giugno, 1999, L'Aquila, Italia.

5. 9th ESCMID Postgraduate Education Course: "The role of antibiotic resistance

surveillance for effective antibiotic policies", 9-11 dicembre, 1999, Verona, Italia.

6. European Union Metallo--Lactamase Network Annual Meeting, 7-9 aprile, 2000,

Copenhagen, Danimarca.

7. International Seminar: “Protein purification: What to do and how?”, 5-9 giugno, 2000,

Liège, Belgio.

8. European Union Metallo--Lactamase Network Mid-Term Review Meeting, 6-7 ottobre,

2000, Liegi, Belgio.

9. 3rd International Meeting on the Therapy of Infections, 4-6 dicembre, 2000, Firenze,

Italia.

10. European Union Metallo--Lactamase Network Annual Meeting, 30 novembre-2

dicembre, 2001, Siena, Italia.

11. 1st International Meeting on Penems, Carbapenems, and Related Compounds, NEMS'

2001, 13-15 dicembre, 2001, Venezia, Italia.

12. 41st Interscience Conference on Antimicrobial Agents and Chemotherapy, ICAAC, 16-19

dicembre, 2001, Chicago, U. S. A.

13. 8th -Lactamase Workshop, 24-28 marzo, 2002, Holy Island, Northumberland, Regno

Unito.

14. 18th ESCMID Postgraduate Education Course "Diagnostics, Characterization and

Epidemiology of -lactamases", 22-23 aprile, 2002, Orta San Giulio, Italia.

15. 10° Congresso Nazionale SIMMOC, 19-21 giugno, 2002, Siena, Italia.

16. European Union Metallo--Lactamase Network Annual Meeting, 8-9 febbraio, 2003,

Copenhagen, Danimarca.

17. 13th European Congress of Clinical Microbiology and Infectious Diseases, ECCMID, 10-

13 maggio, 2003, Glasgow, Regno Unito.


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18. 43rd Interscience Conference on Antimicrobial Agents and Chemotherapy, 14-17

settembre, 2003, Chicago, U.S.A.

19. 31°Congresso Nazionale della Società Italiana di Microbiologia (SIM), 19-22 ottobre,

2003, Roma, Italia.

20. ESCMID-EU European Conference on “The Role of Research in Combating Antibiotic

Resistance”, 28-30 novembre, 2003, Roma, Italia.

21. European Union COBRA Network Kick-off Meeting, 16-17 aprile, 2004, Parigi, Francia.

22. 14th European Congress of Clinical Microbiology and Infectious Diseases, ECCMID, 1-4

maggio, 2004, Praga, Republica Ceca.

23. European Union Metallo--Lactamase Network Annual Meeting, 5-7 giugno, 2004,

Siena, Italia.

24. 12° Congresso Nazionale SIMMOC, 20-22 giugno, 2004, Genova, Italia.

25. 3rd Symposium on Protein Folding and Stability, 27 agosto 2004, Liège, Belgio.

26. European Union Metallo--Lactamase Network Mid-Term Review Meeting, 25-27

settembre, 2004, Liège, Belgio.

27. 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, 30 ottobre– 2

novembre, 2004, Washington D.C., U.S.A.

28. European Union COBRA Network Annual Meeting, 26-27 novembre, 2004, Madrid,

Spagna.

29. European Union COBRA Network Annual Meeting, 3-4 giugno, 2005, Amsterdam,

Olanda.

30. 9th -Lactamase Workshop, 11-15 giugno, 2005, Leonessa, Italia.

31. ESCMID-APUA European Metallo-β-lactamase Expert Meeting, 14-15 novembre, 2005,

Siena, Italia.

32. 45th Interscience Conference on Antimicrobial Agents and Chemotherapy, 16-19

dicembre, 2005, Washington, U.S.A.

33. European Union COBRA Network Annual Meeting, 24-25 febbraio, 2006, Parigi,

Francia.

34. 16th European Congress of Clinical Microbiology and Infectious Diseases, ECCMID, 1-4

aprile, 2006, Nice, Francia.

35. 3° Congresso Nazionale della Società Italiana di Microbiologia Farmaceutica (SIMIF),

25-27 maggio, 2006, Cagliari, Italia.

36. 36th ESCMID Post-graduate Educational Course, “Detection and Characterisation of

Metallo-β-lactamases”, 26-28 maggio, 2006, Verona, Italia.

37. ESCMID Conference on Extended-Spectrum β-Lactamases, 29-31 maggio, 2006,

Venezia, Italia.


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settembre, 2006, San Francisco, U.S.A.

39. European Union Metallo--Lactamase Network Annual Meeting, 1 dicembre, 2006,

Liegi, Belgio.

40. European Union COBRA Network Annual Meeting, 13-14 marzo, 2007, Lione, Francia.



42. 18th European Congress of Clinical Microbiology and Infectious Diseases, 19-22 aprile,

2008, Barcellona, Spagna.

43. 10th β-lactamase meeting, 31 maggio – 4 giugno, 2008, Eretria, Grecia.

44. 36° Congresso Nazionale della Società Italiana di Microbiologia (SIM), 12-15 ottobre,

2008, Roma, Italia.

45. 7th International Symposium on Antimicrobial Agents and Resistance, 18-20 marzo,

2009, Bangkok, Thailandia.

46. Symposium in honour of Professor Jean-Marie Frère, “Penicillin-recognizing enzymes,

from enzyme kinetics to protein folding”, 1-3 luglio, 2009, Liegi, Belgio.


settembre, 2009, San Francisco, U.S.A.


settembre, 2010, Boston, U.S.A.

49. 21st European Congress of Clinical Microbiology and Infectious Diseases – 27th

International Conference on Chemotherapy, 7-10 maggio, 2011, Milano, Italy.

50. 1st European Conference of Microbiology and Immunology, 12-14 maggio, 2011,

Budapest, Hungary.

51. 11th β-lactamase meeting, 10-14 giugno, 2011, Leonessa, Italia.

52. 51st Interscience Conference on Antimicrobial Agents and Chemotherapy, 17-20


53. 40° congresso della Società Italiana di Microbiologia, 7-10 ottobre, Riccione, Italia.


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Comunicazioni scientifiche (atti di convegni)

1. Docquier J.D., Rossolini G.M., Frère J.M. Overproduction of two metallo--

lactamases, the Chryseobacterium meningosepticum BlaB and the Pseudomonas

aeruginosa VIM-1. International EU-ESF Practical Training Course. "Chemistry of

metals in biological systems", 15-25 maggio, 1999, Louvain-la-Neuve, Belgio.

Abstract book.

2. Docquier J.D., Riccio M.L., Frère J.M., Rossolini G.M. Overproduction of the

Pseudomonas aeruginosa VIM-1 metallo--lactamase in Escherichia coli. International

congress on -lactamases. "-lactamase mediated resistance: molecular aspects

and clinical implications", 17-20 giugno, 1999, L'Aquila, Italia. Abstract book,

abstract A9.

3. Vessilier S., Docquier J.D., Rival S., Frère J.M., Galleni M., Amicosante G.,

Rossolini G.M., Franceschini N. Overproduction and kinetic characterisation of

the Chryseobacterium meningosepticum BlaB metallo--lactamase. 46° Congresso

Nazionale della Società Italiana di Biochimica e Biologia Molecolare, 26-29

settembre, 2001, Siena, Italia. Pubblicato in Italian Biochemical Society Transactions

(2001) 17:72.

4. Pallecchi L., Castagnini C., Riccio M.L., Docquier J.D., Fontana R. Rossolini G.M.

Resistenza ai carbapenemi in Pseudomonas aeruginosa: ruolo delle metallo-β-

lattamasi acquisite. 29° Congresso Nazionale della Società Italiana di

Microbiologia, 7-10 novembre, 2001, Genova, Italia. Abstract book, p. 48.

5. Docquier J.D., Lamotte-Brasseur J., Amicosante G., Galleni M., Frère J.M.,

Rossolini G.M. Comparative kinetic characterization and modelling of the VIM-1

and VIM-2 metallo--lactamases. 1st International Meeting on Penems,

Carbapenems, and Related Compounds, 13-15 dicembre, 2001, Venezia, Italia.

Abstract book, abstract n° 33.

6. Docquier J.D., Luzzaro F., Lombardi G., Amicosante G., Toniolo A., Rossolini

G.M. Integron-borne VIM and IMP metallo--lactamases in Pseudomonas putida

clinical isolates from Northern Italy. 1st International Meeting on Penems,

Carbapenems, and Related Compounds, 13-15 dicembre, 2001, Venezia, Italia.


7. Migliavacca R., Docquier J.D., Amicosante G., Rossolini G.M., Pagani L.

Discriminant test for routine detection of metallo--lactamase producers. 1st

International Meeting on Penems, Carbapenems, and Related Compounds, 13-15

dicembre, 2001, Venezia, Italia. Abstract book, abstract n° 35.


pagina 14 di 31


Rossolini G.M., Franceschini N. Chryseobacterium meningosepticum BlaB

carbapenemase: interaction with -lactams and suicide inhibitors. 1st International

Meeting on Penems, Carbapenems, and Related Compounds, 13-15 dicembre,

2001, Venezia, Italia. Abstract book, abstract n° 39.

9. Mazzariol A., Docquier J.D., Xiaokang L., Cornaglia G. Activities of carbapenems

against porin-deficient strains of Escherichia coli producing a VIM-1 or IMP-1

-lactamase. 1st International Meeting on Penems, Carbapenems, and Related

Compounds, 13-15 dicembre, 2001, Venezia, Italia. Abstract book, abstract n° 41.

10. Docquier J.D., Lamotte-Brasseur J., Amicosante G., Galleni M., Frère J.M.,

Rossolini G.M. Overproduction and comparative kinetics of the VIM-1 and VIM-2

metallo--lactamases. 41st Interscience Conference on Antimicrobial Agents and

Chemotherapy, 16-19 dicembre, 2001, Chicago, U.S.A. Abstract book, abstract n°

1502.

11. Docquier J.D., Thaller M.C., Giulani F., Pantanella F., Garcia-Saez I., Frère J.M.,

Bush K., Rossolini G.M. Post-genomic identification and characterization of a

metallo--lactamase from Caulobacter crescentus. 41st Interscience Conference on

Antimicrobial Agents and Chemotherapy, 16-19 dicembre, 2001, Chicago, U.S.A.


12. Migliavacca R., Docquier J.D., Amicosante G., Rossolini G.M., Pagani L. New test

for phenotypic detection of metallo--lactamase producers. 41st Interscience

Conference on Antimicrobial Agents and Chemotherapy, 16-19 dicembre, 2001,

Chicago, U.S.A. Abstract book, abstract n° 181.

13. Docquier J.D., Rossolini G.M. Emergence and spread of metallo--lactamases.

Lecture. 18th ESCMID Postgraduate Education Course "Diagnostics,

Characterization and Epidemiology of -lactamases", 22-23 aprile, 2002, Orta San

Giulio, Italia.

14. Docquier J.D., Castagnini M., Lamotte-Brasseur J., Rossolini G.M. Eterogeneità

funzionale della metallo--lattamasi di tipo VIM: identificazione dei residui

importanti per la specificità di substrato. 10° Congresso Nazionale SIMMOC, 19-

21 giugno, 2002, Siena, Italia. Pubblicato in GIMMOC (2002) 6:122.

15. Luzzaro F., Docquier J.D., Mugnaioli C., Endimiani A., Amicosante G., Rossolini

G.M., Toniolo A. Prevalence of metallo--lactamases in clinical isolates of

Pseudomonas aeruginosa: a one-year survey. 42nd Interscience Conference on

Antimicrobial Agents and Chemotherapy, 27-30 settembre, 2002, San Diego,

U.S.A. Abstract book, abstract n° C2-1892.


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16. Docquier J.D., Riccio M.L., Mugnaioli C., Luzzaro F., Toniolo A., Amicosante G.,

Rossolini G.M. IMP-12: a new plasmid-encoded IMP variant highly divergent

from IMP-1. 42nd Interscience Conference on Antimicrobial Agents and

Chemotherapy, 27-30 settembre, 2002, San Diego, U.S.A. Abstract book, abstract

n° C1-1847.

17. Docquier J.D., Mugnaioli C., Luzzaro F., Amicosante G., Toniolo A., Rossolini G.

M.. Diversity of IMP-type metallo--lactamases in carbapenem-resistant clinical

isolates of Pseudomonas spp. from Italy. 13th European Congress of Clinical

Microbioliogy and Infectious Diseases, 10-13 maggio, 2003, Glasgow, Regno

Unito. Abstract book, abstract P583. Pubblicato in Clinical Microbiology and

Infection (2003) 9 (Suppl. 1):121.

18. Luzzaro F., Docquier J.D., Colinon C., Endimiani A., Amicosante G., Rossolini

G.M., Toniolo A. First detection of Klebsiella pneumoniae and Enterobacter cloacae

clinical isolates producing the VIM-1 metallo--lactamase. 13th European

Congress of Clinical Microbioliogy and Infectious Diseases, 10-13 maggio, 2003,

Glasgow, Regno Unito. Abstract book, abstract P1052. Pubblicato in Clinical

Microbiology and Infection (2003) 9 (Suppl. 1):246.

19. Migliavacca R., Docquier J.D., Spalla M., Pallecchi L., Nucleo E., Giacobone E.,

Zara F., Brerra R., Rossolini G.M., Pagani L. Nosocomial infections caused by

Pseudomonas aeruginosa with acquired blaVIM metallo--lactamase genes in a

hospital from Northern Italy. 13th European Congress of Clinical Microbioliogy

and Infectious Diseases, 10-13 maggio, 2003, Glasgow, Regno Unito. Abstract

book, abstract P1211. Pubblicato in Clinical Microbiology and Infection (2003) 9

(Suppl. 1):287.

20. Docquier J.D., Mugnaioli C., Perilli M., Amicosante G., Rossolini G.M.

Biochemical characterization of IMP-13, a new IMP variant produced by a

Pseudomomas aeruginosa clinical isolate from Italy. 43rd Interscience Conference on

Antimicrobial Agents and Chemotherapy, 14-17 settembre, 2003, Chicago, U.S.A.

Abstract book, abstract n° C1-660.

21. Docquier J.D., Lamotte-Brasseur J., Amicosante G., Frère J.M., Rossolini G.M.

Functional heterogeneity of VIM-type metallo--lactamases: identification of

residues critical to substrate binding. 43rd Interscience Conference on



22. Lopizzo T., D'Andrea M., Thaller M.C., Amicosante G., Rossolini G.M., Docquier

J.D. Purification and biochemical characterization of the THIN-B metallo--

lactamase from the environmental bacterium Janthinobacterium lividum. 43rd

Interscience Conference on Antimicrobial Agents and Chemotherapy, 14-17

settembre, 2003, Chicago, U.S.A. Abstract book, abstract n° C1-670.


pagina 16 di 31

23. Giuliani F., Docquier J.D., Riccio M.L., Pagani L., Rossolini G.M. Identification

and biochemical characterization of OXA-46, a new integron-encoded OXA-type

-lactamase from a Pseudomonas aeruginosa clinical isolate. 43rd Interscience

Conference on Antimicrobial Agents and Chemotherapy, 14-17 settembre, 2003,

Chicago, U.S.A. Abstract book, abstract n° C1-1183.

24. Riccio M.L., Pallecchi L., Docquier J.D., Cresti S., Fontana R., Pagani L., Monti-

Bragadin C., Rossolini G. M. Clonal relatedness of Pseudomonas aeruginosa

producing the VIM-1 metallo--lactamase from different Italian hospitals. 43rd



25. Docquier J.D., Mugnaioli C., Amicosante G., Rossolini G.M. Diversity of IMP-

type metallo--lactamases in Italy: characterization of IMP-13, a new highly

divergent variant. 31° Congresso Nazionale della Società Italiana di

Microbiologia, 19-22 ottobre, 2003, Roma, Italia.

26. Luzzaro F., Docquier J.D., Endimiani A., Colinon C., Lombardi G., Amicosante

G., Rossolini G.M., Toniolo A. First detection of Klebsiella pneumoniae and

Enterobacter cloacae clinical isolates producing the VIM-1 metallo--lactamase. 31°

Congresso Nazionale della Società Italiana di Microbiologia, 19-22 ottobre, 2003,

Roma, Italia.

27. Docquier J.D., Stoczko M., Amicosante G., Frère J.M., Rossolini G.M. Post-

genomic identification of a new subclasss B3 metallo--lactamase (NOV-1) from

Novosphingobium aromaticivorans. 14th European Congress of Clinical

Microbioliogy and Infectious Diseases, 1-4 maggio, 2004, Praga, Republica Ceca.

Abstract book, abstract P1460. Pubblicato in Clinical Microbiology and Infection

(2004) 10 (Suppl. 3):410.

28. Docquier J.D., Mugnaioli C., Luzzaro F., Musmanno R.A., Rossolini G.M. New

aminoglycoside and chloramphenicol acetyltransferase-encoding gene cassettes

in class 1 integrons from multiresistant Pseudomonas aeruginosa clinical isolates.

14th European Congress of Clinical Microbioliogy and Infectious Diseases, 1-4

maggio, 2004, Praga, Republica Ceca. Abstract book, abstract P1461. Pubblicato in

Clinical Microbiology and Infection (2004) 10 (Suppl. 3):410.

29. Migliavacca R., Colinon C., Docquier J.D., Spalla M., Nucleo E., Li Bergoli M.,

Labonia M., Rossolini G.M., Pagani L. Outbreak of multiresistant Pseudomonas

aeruginosa producing the metallo--lactamase IMP-13 in a general intensive care

unit in southern Italy. 14th European Congress of Clinical Microbioliogy and

Infectious Diseases, 1-4 maggio, 2004, Praga, Republica Ceca. Abstract book,

abstract P1876. Pubblicato in Clinical Microbiology and Infection (2004) 10 (Suppl.

3):537.


pagina 17 di 31

30. Labonia M., Li Bergoli M., Migliavacca R., Colinon C., Docquier J.D., Spalla M.,

Nucleo E., Rossolini G.M., Pagani L. Epidemia di Pseudomonas aeruginosa

multiresistenti, produttori di metallo--lattamasi IMP-13, in una unità di terapia

intensiva dell’IRCSS “Casa Sollievo della Sofferenza” di San Giovanni Rotondo

(FG). 33° Congresso Nazionale dell’Associazione dei Microbiologi Clinici Italiani

(AMCLI), 8-11 giugno, 2004, Padova, Italia. Pubblicato in Microbiologia Medica

(2004) 19:193.

31. Docquier J.D., Borgianni L., Frère J.M., Rossolini G.M. Analisi della relazione

struttura-funzione della metallo--lattamasi VIM-2 tramite mutagenesi a

saturazione: ruolo della tasca idrofobica del sito catalitico nell’attività enzimatica.

12° Congresso Nazionale SIMMOC, 20-22 giugno, 2004, Genova, Italia.

32. Iannelli F., Santagati M., Docquier J.D., Cassone M., Oggioni M.R., Rossolini

G.M., Stefani S., Pozzi G. La resistenza di tipo M ai macrolidi negli Streptococchi

non è dovuto al gene mefA ma al gene matA che codifica una pompa ad efflusso

ATP-dipendente. 12° Congresso Nazionale SIMMOC, 20-22 giugno, 2004,

Genova, Italia.


G.M., Stefani S., Pozzi G. La resistenza di tipo M ai macrolidi negli streptococchi è

dovuta ad un sistema di efflusso della superfamiglia dei trasportatori ABC (ATP-

binding cassette). 32° Congresso Nazionale Società Italiana di Microbiologia

(SIM), Milano, 26-29 settembre 2004.

34. Docquier J.D., Longatti A., Amicosante G., Frère J.M., Rossolini G.M. Post-

genomic identification and characterization of a new subclass B2 metallo--

lactamase encoded by the chromosome of Chromobacterium violaceum. 44th

Interscience Conference on Antimicrobial Agents and Chemotherapy, 30 ottobre-

2 novembre, 2004, Washington, U.S.A. Abstract book, abstract n° C1-289.

35. Docquier J.D., Borgianni L., Frère J.M., Rossolini G.M. Structure-function

relationship analysis of the VIM-2 metallo--lactamase by saturation mutagenesis:

role of the active site hydrophobic pocket in enzyme activity. 44th Interscience

Conference on Antimicrobial Agents and Chemotherapy, 30 ottobre- 2 novembre,

2004, Washington, U.S.A. Abstract book, abstract n° C1-963.


G.M., Stefani S., Pozzi G. Type M resistance to macrolides in Streptococci is not

due to the mefA gene, but to matA encoding an ATP-dependent efflux pump. 44th

Interscience Conference on Antimicrobial Agents and Chemotherapy, 30 ottobre-

2 novembre, 2004, Washington, U.S.A. Abstract book, abstract n° C1-1188.

37. Stoczko M, Frère J.M., Rossolini G.M., Docquier J.D. JAP-1, a new member of

subclass B3 metallo-β-lactamase from Bradyrhizobium japonicum. 15th European

Congress of Clinical Microbiology and Infectious Diseases, 2-5 aprile, 2005,

Copenhagen, Danimarca. Abstract book, abstract P411. Pubblicato in Clinical



pagina 18 di 31

38. De Santis F., Caporale B., Celenza G., Fiore M., Pellegrini C., Perilli M., Docquier

J.D., Rossolini G.M., Amicosante G. Distribution of β-lactamases in clinical

isolates of Enterobacteriaceae and nonfermenters from a region of central Italy. 15th

European Congress of Clinical Microbiology and Infectious Diseases, 2-5 aprile,

2005, Copenhagen, Danimarca. Abstract book, abstract P790. Pubblicato in Clinical


39. Rossolini G.M., Docquier J.D. Metallo-β-lactamases: a last frontier for β-lactams.

15th European Congress of Clinical Microbiology and Infectious Diseases, 2-5

aprile, 2005, Copenhagen, Danimarca. Abstract book, abstract K371. Pubblicato in

Clinical Microbiology and Infection (2005) 11 (Suppl. 2):88-89.


G.M., Stefani S., Pozzi G. Type M resistance to macrolides is due to an efflux

transport system of the ATP-binding cassette (ABC) superfamily.

EUROPNEUMO, 8-11 maggio 2005, Braunschweig, Germania. Abstract book,

abstract n° O4.2.

41. Voha C., Docquier J.D., Rossolini G.M., Fosse T. Biochemical characterization of

FUS-1, a narrow-spectrum class D β-lactamase from a Fusobacterium nucleatum

polymorphum clinical isolate. 45th Interscience Conference on Antimicrobial Agents

and Chemotherapy, 16-19 dicembre, 2005, Washington, U.S.A. Abstract book,

abstract n° C1-73.

42. Calderone V., Giuliani F., Benvenuti M., Docquier J.D., Nordmann P., Mangani

S., Rossolini G.M. Structural and functional characterization of OXA-48 β-

lactamase: first structure of a class D carbapenemase. 45th Interscience Conference

on Antimicrobial Agents and Chemotherapy, 16-19 dicembre, 2005, Washington,


43. Zéba B., Simporé J., Nacoulma O.G., Rossolini G.M., Frère J.M., Docquier J.D.

Characterization of a new IND variant from Chryseobacterium indologenes: first

report of a metallo-β-lactamase-producing clinical isolate from Africa. 45th


dicembre, 2005, Washington, U.S.A. Abstract book, abstract n° C1-85.

44. Docquier J.D., Bundone L., Frère J.M., Streit W.R., Rossolini G.M. Biochenical

characterization of ILM-1, a new IMP-like metallo-β-lactamase from a soil

metagenome. 45th Interscience Conference on Antimicrobial Agents and

Chemotherapy, 16-19 dicembre, 2005, Washington, U.S.A. Abstract book, abstract

n° C1-86.

45. Stoczko M., Frère J.M., Rossolini G.M., Docquier J.D. Metallo-β-lactamase

homologues in the Rhizobacteria: Identification of new subclass B3 metallo-β-

lactamases. 45th Interscience Conference on Antimicrobial Agents and

Chemotherapy, 16-19 dicembre, 2005, Washington, U.S.A. Abstract book, abstract

n° C1-87.


pagina 19 di 31

46. Docquier J.D., Prandi S., Cresti S., Rossolini G.M. Biochemical characterization of

the plasmid-encoded class C β-lactamase FOX-7. 16th European Congress of

Clinical Microbioliogy and Infectious Diseases, 1-4 aprile, 2006, Nizza, Francia.


(2006) 12 (Suppl. 4):137.

47. Stoczko M., Frère J.M., Rossolini G.M., Docquier J.D. Post-genomic detection of

CAR-1, a new subclass B3 metallo-β-lactamase from the important plant pathogen

Erwinia carotovora. 16th European Congress of Clinical Microbiology and Infectious

Diseases, 1-4 aprile, 2006, Nizza, Francia. Abstract book, abstract P926. Pubblicato

in Clinical Microbiology and Infection (2006) 12 (Suppl. 4):285-286.

48. Docquier J.D., D’Andrea M.M., Christiaens G., Boreux R., Giani T., Frère J.M., De

Mol P., Rossolini G.M. Genetic structure of a VIM-2-encoding integron from a

Pseudomonas aeruginosa clinical isolate from Belgium. 16th European Congress of

Clinical Microbiology and Infectious Diseases, 1-4 aprile, 2006, Nizza, Francia.


(2006) 12 (Suppl. 4):287.

49. Perilli M., Segatore B., Caporale B., Pellegrini C., Celenza G., De Santis F., Fiore

M., Luzi C., Docquier J.D., Rossolini G.M., Amicosante G. Biochemical

characterisation of IND-NF16 metallo-β-lactamase. 16th European Congress of

Clinical Microbiology and Infectious Diseases, 1-4 aprile, 2006, Nizza, Francia.


(2006) 12 (Suppl. 4):288-289.

50. Borgianni L., Frère J.M., Coratza G., Rossolini G.M., Docquier J.D. Analisi della

relazione struttura-funzione della metallo-β-lattamasi VIM-2 tramite mutagenesi

a saturazione: ruolo della tasca idrofobica del sito catalitico nell’attività

enzimatica. III° Congresso Nazionale della Società Italiana di Microbiologia

Farmaceutica (SIMIF), 25-27 maggio, 2006, Cagliari, Italy. Abstract book.

51. Docquier J.D., Prandi S., Cresti S., Rossolini G.M. Caratterizzazione biochimica

della β-lattamasi di classe C di origine plasmidica FOX-7. III° Congresso

Nazionale della Società Italiana di Microbiologia Farmaceutica (SIMIF), 25-27

maggio, 2006, Cagliari, Italy. Abstract book.

52. Oggioni M.R., Pettini E., Camilli R., Paterson G. K., Del Grosso M., Docquier J.D.,

Athwal J., Mitchell T.J., Kilian M., Pantosti A., Pozzi G. Pneumococcal zinc

metalloproteinases: epidemiology, virulence, and processing. ASM Conference on

Streptococcal Genetics. 18-21 giugno 2006, Saint-Malo, France.

53. Calderone V., Giuliani F., Benvenuti M., Docquier J.D., Nordmann P., Rossolini

G.M., Mangani S. Structural and functional characterization of two new β-

lactamases: OXA-46 and OXA-48. 23rd European Crystallographic Meeting, 6-11

agosto, 2006, Leuven, Belgium. Abstract book, abstract m11.p02. Pubblicato in

Acta Cryst. (2006) A62:s161.


pagina 20 di 31

54. Calderone V., Giuliani F., Benvenuti M., Docquier J.D., Rossolini G.M., Mangani

S. The uneasy task to correlate structure to function: the case of class D β-

lactamases. XXXV Annual Meeting of the Italian Association of Crystallography,

18-21 settembre, 2006, Ferrara, Italy. Abstract book.

55. Voha C., Calderone V., Benvenuti M., Mangani S., Rossolini G.M., Fosse T.,

Docquier J.D. Crystal structure and mutational analysis of FUS-1 (OXA-85), a

class D β-lactamase from Fusobacterium nucleatum subsp. polymorphum. 46th


settembre, 2006, San Francisco, U.S.A. Abstract book, abstract n° C1-26.

56. Borgianni L., Frère J.M., Rossolini G.M., Docquier J.D. Mutational analysis of the

VIM-2 active site: role of positions 64 and 87 in enzyme activity and stability. 46th



57. Docquier J.D., De Luca F., Lee K., Chong Y., Rossolini G.M. Biochemical

characterization of SIM-1, a new integron-borne subclass B1 metallo-β-lactamase

from Acinetobacter baumannii isolates from Korea. 46th Interscience Conference on

Antimicrobial Agents and Chemotherapy, 27-30 settembre, 2006, San Francisco,


58. Christiaens G., Damas P., Docquier J.D., Hayette M., Melin P., De Mol P.

Molecular epidemiology of extended-spectrum β-lactamases-producing

Enterobacteriaceae colonizing the digestive tract of patients admitted to intensive

care units in a Belgian university hospital. 46th Interscience Conference on

Antimicrobial Agents and Chemotherapy, 27-30 settembre, 2006, San Francisco,


59. Helfand M.S., Taracila M.A., Borgianni L., Docquier J.D., Venkatesan A.M.,

Mansour T.S. Inhibition of Class A, B and C β-lactamases (βLs) by Bicyclic 6-

Methylidene Penems via Michaelis Complexes and Cyclic Enamines. 47th



60. Giraud-Morin C., De Luca F., Rossolini G.M., Docquier J.D., Fosse T. Genetic and

Biochemical Characterization of TRU-1, the Endogenous Class C β-lactamase

from Aeromonas trota. 47th Interscience Conference on Antimicrobial Agents and

Chemotherapy, 17-20 settembre, 2007, Chicago, U.S.A. Abstract book, abstract n°

C1-592.

61. Mereuta A.I., Buiuc, D.T., Rossolini G.M, Docquier J.D. Simultaneous Detection

of VIM-2 and IMP-13-Producing Carbapenem-Resistant Pseudomonas aeruginosa

Clinical Isolates from Romania. 47th Interscience Conference on Antimicrobial

Agents and Chemotherapy, 17-20 settembre, 2007, Chicago, U.S.A. Abstract book,

abstract n° C2-1498.


pagina 21 di 31

62. Docquier J.D., Xerri L. Interaction of PZ-601 (SMP-601) and other β-lactams with

chromosome- and plasmid-encoded class C β-lactamases. 18th European Congress

of Clinical Microbiology and Infectious Diseases, 19-22 aprile, 2008, Barcelona,

Spagna. Abstract book, P566.

63. Docquier J.D., Xerri L. Stability of PZ-601 (SMP-601) to different clinically-

relevant extended-spectrum β-lactamases. 18th European Congress of Clinical

Microbiology and Infectious Diseases, 19-22 aprile, 2008, Barcelona, Spagna.

Abstract book, P565.

64. Borgianni L., Matagne A., Frère J.M., Rossolini G.M., Docquier J.D. Structure-

function relationship analysis of the VIM-2 metallo-β-lactamase: identification of

a residue critical to enzyme structure. 10th β-lactamase meeting, 31 maggio – 4

giugno, 2008, Eretria, Grecia.

65. De Luca F., Rossolini G.M., Fosse T., Docquier J.D. Biochemical characterization

of TRU-1, the endogenous class C β-lactamase from Aeromonas trota. 10th β-

lactamase meeting, 31 maggio – 4 giugno, 2008, Eretria, Grecia.

66. Oggioni M.R., Athwal J., Trappetti C., Pozzi G., Docquier J.D., Andrew P.W.

Enzyme kinetics and substrate specificity of neuraminidases A, B and C of

Streptococcus pneumoniae. 6th International Symposium on Pneumococci &

Pneumococcal Diseases, 8-12 giugno 2008, Reykjavik, Islanda.

67. Docquier J.D., De Luca F., Giuliani F., Calderone V., Benvenuti M., Nordmann P.,

Mangani S., Rossolini G.M. Structural and functional analysis of the OXA-48 class

D carbapenemase: implications for the catalytic mechanism. 36° Congresso

Nazionale della SIM, 12-15 ottobre, 2008, Roma, Italia.

68. Voha C., Docquier J. D., Bonnaure-Mallet M., Kononen E., Fosse T. Antibiotic

susceptibility and distribution of β-lactamases in Fusobacterium species. 48th

Interscience Conference on Antimicrobial Agents and Chemotherapy / ISDA 46th

Annual Meeting, 25-28 ottobre, 2008, Washington DC, U.S.A. Abstract book,


69. Borgianni L., De Luca F., Rossolini G.M., Docquier J.D. ME-1071, a novel metallo-

β-lactamase inhibitor: inhibition mechanism and in vitro synergistic activity

against MBL-producing gram-negative pathogens. 48th Interscience Conference

on Antimicrobial Agents and Chemotherapy / ISDA 46th Annual Meeting, 25-28

ottobre, 2008, Washington DC, U.S.A. Abstract book, abstract n° F1-1170.

70. Borgianni L., Prandi S., Salden L., Hanson N.D., Rossolini G.M., Docquier J.D.

Genetic and biochemical characterization of IMP-18, a MBL determinant

identified in a Pseudomonas aeruginosa isolate from the U.S.A. 7th International

Symposium on Antimicrobial Agents and Resistance, 18-20 marzo, 2009,

Bangkok, Thailandia. Abstract book, abstract n° GN 099.


pagina 22 di 31

71. Poirel L., Docquier J.D., De Luca F., Verlinde A., Ide L., Rossolini G.M.,

Nordmann P. BEL-2, an expanded-spectrum β-lactamase with increased activity

toward broad-spectrum cephalosporins in Pseudomonas aeruginosa. 19th European

Congress of Clinical Microbiology and Infectious Diseases, 16-19 maggio, 2009,

Helsinki, Finlandia. Abstract book, abstract P1485.

72. Docquier J.D. Structure and function of class D carbapenemases. Symposium in

honour of Professor Jean-Marie Frère, “Penicillin-recognizing enzymes, from

enzyme kinetics to protein folding”, 1-3 luglio, 2009, Liège, Belgio.

73. Borgianni L., Prandi S., Salden L., Santella G., Hanson N.D., Rossolini G.M.,

Docquier J.D. Genetic and biochemical characterization of IMP-18, a MBL

determinant identified in a Pseudomonas aeruginosa isolate from the U.S.A. 49th



74. Docquier J.D., Stachyra T., Benvenuti M., Rossolini G.M., Mangani S., Pechereau

M.C., Bruneau J.M., Claudon M., Barbosa F., Miossec C., Black M.T. High

resolution crystal structure of CTX-M-15 in complex with the new β-lactamase

inhibitor NXL104. 49th Interscience Conference on Antimicrobial Agents and

Chemotherapy, 12-15 settembre, 2009, San Francisco, U.S.A. Abstract book,


75. Borgianni L., Vandenameele J., Matagne A., Bonomo R.A., Frère J.M., Rossolini

G.M., Docquier J.D. Tryptophan 87 of the VIM-2 metallo-β-lactamase is essential

for stability, in vivo folding and β-lactam resistance. 49th Interscience Conference

on Antimicrobial Agents and Chemotherapy, 12-15 settembre, 2009, San

Francisco, U.S.A. Abstract book, abstract n° C1-1369.

76. Morinaka A., Ohkuma K., Matsumoto K., Mikuniya T., Sakakibara S., Maebashi

K., Suzuki H., Docquier J.D., Rossolini G.M. In vivo efficacy of ME1071, a novel

metallo-β-lactamase (MBL) inhibitor, in combination with carbapenems against

MBL-producing Pseudomonas aeruginosa in a murine pneumonia model. 49th


settembre, 2009, San Francisco, U.S.A. Abstract book, abstract n° F1-1491.

77. De Luca F., Benvenuti M., Carboni F., Rossolini G.M., Docquier J.D., Mangani S.

Evolution of a β-lactamase by rational protein design and loop grafting: changing

an oxacillinase in a carbapenemase. 2nd Meeting of the Italian and Spanish

Crystallographic Associations, 30 giugno – 3 luglio, 2010, Oviedo, Spain.

78. Docquier J.D., Benvenuti M., De Luca F., Rossolini G.M., Mangani S., Miossec C.,

Black T.M. X-ray crystal structure of the Klebsiella pneumoniae OXA-48 class D

carbapenemase inhibited by NXL104. 50th Interscience Conference on

Antimicrobial Agents and Chemotherapy, 12-15 settembre, 2010, Boston, U.S.A.



pagina 23 di 31

79. De Luca F., Benvenuti M., Carboni F., Rossolini G.M., Mangani S., Docquier J.D.

Evolution of a β-lactamase by rational protein design and loop grafting: changing

an oxacillinase in a carbapenemase. 50th Interscience Conference on

Antimicrobial Agents and Chemotherapy, 12-15 settembre, 2010, Boston, U.S.A.

Abstract book, abstract C1-646.


Black T.M. Structure of the TRU-1 class C and OXA-10 class D β-lactamases in

complex with NXL104: structural basis for broad-spectrum inhibition. 50th


settembre, 2010, Boston, U.S.A. Abstract book, abstract n° C1-1427.

81. Santela G., Pollini S., Docquier J.D., Mereuta A.I., Gutkind G., Rossolini G.M.,

Radice M. Global spread of IMP-13-producing Pseudomonas aeruginosa sequence

type 621. 50th Interscience Conference on Antimicrobial Agents and

Chemotherapy, 12-15 settembre, 2010, Boston, U.S.A. Abstract book, abstract n°

C2-1804.


Black T.M. Structure of the TRU-1 class C and OXA-10 class D β-lactamases in

complex with NXL104: structural basis for broad-spectrum inhibition. Advances

in Protein Crystallography, 9-10 novembre, 2010, Firenze, Italia.

83. Docquier J.D., Benvenuti M., Bruneau J.M., Rossolini G.M., Mangani S., Miossec

C., Black M.T. X-ray Crystal Structure of the Acinetobacter baumannii OXA-24/40

Class D Carbapenemase Inhibited by NXL104. 21st European Congress of Clinical

Microbiology and Infectious Diseases/27th International Congress of

Chemotherapy and Infection (Joint ECCMID-ICC meeting), 7-10 maggio 2011,

Milano, Italia.

84. Docquier J.D. Clinically-relevant β-lactamases: Structure-function relationships

and inhibition. 1st European Conference of Microbiology and Immunology, 12-14

maggio, 2011, Budapest, Hungary.

85. Borgianni L., Giacobini G., Rossolini G.M., Docquier J.D. Structure-fucntion

relationship of the NDM-1 metallo-beta-lactamase: Importance of Trp-87 in

enzyme structure, function and stability. 51st Interscience Conference on



86. Cejas D., De Luca F., Gutkind G., Rossolini G.M., Radice M., Docquier J.D.

Probing the role of R2-loop deletions and substituions on the functional

properties of the acquired AmpC-type β-lactamase CMY-2. 51st Interscience

Conference on Antimicrobial Agents and Chemotherapy, 17-20 settembre, 2011,

Chicago, U.S.A. Abstract book, abstract n° C1-607.

87. Docquier J.D. Structure and function of metallo-β-lactamases. Meet-the-Experts

session “Structure, Function and Genetics of Metallo-β-Lactamases”. 51st


pagina 24 di 31


settembre, 2011, Chicago, U.S.A. Abstract book, abstract n° 1038.

88. Di Lorenzo V., Decorosi F., Gualdi L., Docquier J.D., Giovannetti L., Viti C.,

Oggioni M.R. Phenotypic profiling of Pseudomonas aeruginosa planktonic growth,

biofilm formation and fluorescence. SIMGBM 29th National Meeting, 21-23

settembre, 2011, Pisa, Italia.

89. Docquier J.D. Antibiotico-resistenza e Batteri ambientali. 40° congresso della

Società Italiana di Microbiologia, 7-10 ottobre, 2012, Riccione, Italia.


pagina 25 di 31

Pubblicazioni in extenso

1. Pierrard A., Ledent P., Docquier J.D., Feller G., Gerday C., Frère J.M. Inducible

class C -lactamases produced by psychrophilic bacteria. FEMS Microbiol. Lett.

(1998) 161:311-315.

2. Franceschini N., Caravelli B., Docquier J.D., Galleni M., Frère J.M., Amicosante

G., Rossolini G.M. Purification and biochemical characterization of the VIM-1

metallo--lactamase. Antimicrob. Agents Chemother. (2000) 44:3003-7.

3. Pallecchi L., Riccio M.L., Docquier J.D., Fontana R., Rossolini G.M. Molecular

heterogeneity of blaVIM-2-containing integrons from Pseudomonas aeruginosa

plasmids encoding the VIM-2 metallo--lactamase. FEMS Microbiol. Lett. (2001)

195:145-50.

4. Rossolini G.M., Condemi M.A., Pantanella F., Docquier J.D., Amicosante G.,

Thaller M.C. Metallo--lactamase producers in environmental microbiota: new

molecular class B enzyme in Janthinobacterium lividum. Antimicrob. Agents

Chemother. (2001) 45:837-44.

5. Duez C., Vanhove M., Gallet X., Bouillenne F., Docquier J.D., Brans A., Frère J.-

M. Purification and characterization of PBP4a, a new low-molecular-weight

penicillin-binding protein from Bacillus subtilis. J. Bacteriol. (2001) 183:1595-9.

6. Bebrone C., Moali C., Mahy F., Rival S., Docquier J.D., Rossolini G.M., Fastrez J.,

Pratt R.F., Frère J.M., Galleni M. CENTA as a chromogenic substrate for studying

-lactamases. Antimicrob. Agents Chemother. (2001) 45:1868-71.

7. Docquier J.D., Luzzaro F., Amicosante G., Toniolo A., Rossolini G.M. Emergence

of multidrug-resistant Pseudomonas aeruginosa producing the PER-1 extended-

spectrum serine--lactamase and the VIM-2 metallo--lactamase. Emerg. Infect.

Dis. (2001) 7:910-911.

8. Docquier J.D., Pantanella F., Giuliani F., Thaller M.C., Amicosante G., Galleni M.,

Frère J.M., Bush K., Rossolini G.M. CAU-1, a subclass B3 metallo--lactamase of

low substrate affinity encoded by an ortholog present in the Caulobacter crescentus

chromosome. Antimicrob. Agents Chemother. (2002) 46:1823-30.


Rossolini G.M., Franceschini N. Overproduction and biochemical characterization

of the Chryseobacterium meningosepticum BlaB metallo--lactamase. Antimicrob.

Agents Chemother. (2002) 46:1921-27.

10. Lombardi G., Luzzaro F., Docquier J.D., Riccio M.L., Perilli M., Coli A.,

Amicosante G., Rossolini G.M., Toniolo A. Nosocomial infections caused by

multidrug-resistant isolates of Pseudomonas putida producing the VIM-1 metallo-

-lactamase. J. Clin. Microbiol. (2002) 40:4051-4055.


pagina 26 di 31

11. Migliavacca R., Docquier J.D., Mugnaioli C., Amicosante G., Daturi R., Lee K.,

Rossolini G.M.. Pagani, L. Simple microdilution test for detection of metallo--

lactamase production in Pseudomonas aeruginosa. J. Clin. Microbiol. (2002) 40:4388-

4390.

12. Pallecchi L., Docquier J.D., Riccio M.L., Giuliani F., Cresti S., Musmanno R.A.,

Coratza G., Rossolini G.M. Carbapenemases in microbial drug resistance. L'Igiene

Moderna. (2003) 120:285-298.

13. Docquier J.D., Lamottte-Brasseur J., Galleni M., Amicosante G., Frère J.M.,

Rossolini G.M. On functional and structural heterogeneity of VIM-type metallo--

lactamases. J. Antimicrob. Chemother. (2003) 51:257-266.

14. Docquier J.D., Riccio M.L., Mugnaioli C., Luzzaro F., Endimiani A., Toniolo, A.,

Amicosante G., Rossolini G.M. IMP-12, a new plasmid-encoded metallo--

lactamase of the IMP-type from a Pseudomonas putida isolate. Antimicrob. Agents

Chemother. (2003) 47:1522-1528.

15. Riccio M.L., Docquier J.D., Dell’Amico E., Luzzaro F., Amicosante G., Rossolini

G.M. Characterization of a novel 3-N-aminoglycoside acetyltransferase gene,

aac(3)-Ic, from a Pseudomonas aeruginosa integron. Antimicrob. Agents Chemother.

(2003) 47:1746-1748.

16. Forleo C., Benvenuti M., Calderone V., Schippa S., Docquier J.D., Thaller M.C.,

Rossolini G.M., Mangani S. Expression, purification, crystallization and

preliminary X-ray characterization of the class B acid phosphatase (AphA) from

Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. (2003) 59:1058-1060.

17. Luzzaro F., Endimiani A., Docquier J.D., Mugnaioli C., Bonsignori M.,

Amicosante G., Rossolini G.M. Prevalence and characterization of metallo--

lactamases in clinical isolates of Pseudomonas aeruginosa. Diagn. Microbiol. Infect.

Dis. (2004) 48:131-135.

18. Luzzaro F., Docquier J.D., Colinon C., Endimiani A., Lombardi G., Amicosante

G., Rossolini G.M., Toniolo A. Emergence in Klebsiella pneumoniae and Enterobacter

cloacae clinical isolates of the VIM-4 metallo--lactamase encoded by a conjugative

plasmid. Antimicrob. Agents Chemother. (2004) 48:648-650.

19. Docquier J.D., Lopizzo T., Liberatori S., Prenna M., Thaller M.C., Frère J.M.,

Rossolini G.M. Biochemical characterization of the THIN-B metallo--lactamase

of Janthinobacterium lividum. Antimicrob. Agents Chemother. (2004) 48:4778-4783.

20. Riccio M.L., Pallecchi L., Docquier J.D., Cresti S., Catania M.R., Pagani L.,

Lagatolla C., Cornaglia G., Fontana R., Rossolini G.M. Clonal relatedness and

conserved integron structures in epidemiologically unrelated Pseudomonas

aeruginosa producing the VIM-1 metallo--lactamase from different Italian

hospitals. Antimicrob. Agents Chemother. (2005) 49:104-110.


pagina 27 di 31

21. Giuliani F., Docquier J.D., Riccio M.L., Pagani L., Rossolini G.M. OXA-46, a new

class D β-lactamase of narrow substrate specificity, encoded by a blaVIM-1-

containing integron from a Pseudomonas aeruginosa clinical isolate. Antimicrob.


22. Pagani L., Colinon C., Migliavacca R., Labonia M., Docquier J.D., Nucleo E.,

Spalla M., Li Bergoli M., Rossolini G.M. Nosocomial outbreak caused by

multidrug-resistant Pseudomonas aeruginosa producing the IMP-13 metallo-β-

lactamase. J. Clin. Microbiol. (2005) 43:3824-3828.

23. Lee K., Yum J.H., Yong D., Lee H.M., Kim H.D., Docquier J.D., Rossolini G.M.,

Chong Y. A novel acquired Metallo-β-lactamase gene, blaSIM-1, in a class 1 integron

from Acinetobacter baumannii clinical isolates from Korea. Antimicrob. Agents

Chemother. (2005) 49:4485-4491.

24. Stoczko M., Frère J.M., Rossolini G.M., Docquier J.D. Post-genomic scan of

metallo-β-lactamase homologues in Rhizobacteria: identification and

characterization of BJP-1, a subclass B3 ortholog from Bradyrhizobium japonicum.

Antimicrob. Agents Chemother. (2006) 50:1973-1981.

25. Voha C., Docquier J.D., Rossolini G.M., Fosse T. Genetic and biochemical

characterization of FUS-1 (OXA-85), a narrow-spectrum class D β-lactamase from

Fusobacterium nucleatum subsp. polymorphum. Antimicrob. Agents Chemother. (2006)

50:2673-2679.

26. Rossolini G.M., Docquier J.D. New β-lactamases: a paradigm for the rapid

response of bacterial evolution in the clinical setting. Future Microbiology (2006)

1:295-308.

27. Zeba B., Kiendrébeogo M., Lamien A., Docquier J.D., Simporé J., Nacoulma O.G.

Major enzymatic factors involved in bacterial penicillin resistance in Burkina

Faso. Pak. J. Biol. Sci. (2007) 10:506-510.

28. Cornaglia G., Akova M., Amicosante G., Canton R., Cauda R., Docquier J.D.,

Edelstein M., Frère J.M., Fuzi M., Galleni M., Giamarellou H., Gniadkowski M.,

Koncan R., Libisch B., Luzzaro F., Miriagou V., Navarro F., Nordmann P., Pagani

L., Peixe L., Poirel L., Souli M., Tacconelli E., Vatopoulos A., Rossolini G.M.

Metallo-β-lactamases as emerging resistance determinants in Gram-negative

pathogens: open issues. Int. J. Antimicrob. Agents (2007) 29:380-388.

29. Rossolini G.M., Mantengoli E., Docquier J.D., Musmanno R., Coratza G.

Epidemiology of infections caused by multiresistant gram-negatives: ESBLs,

MBLs, panresistant strains. New Microbiol. (2007) 30:332-339.

30. Perilli M., Caporale B., Celenza G., Pellegrini C., Docquier J.D., Mezzatesta M.,

Rossolini G.M., Stefani S., Amicosante G. Identification and characterization of a

new metallo-β-lactamase, IND-5, from a clinical isolate of Chryseobacterium

indologenes. Antimicrob. Agents Chemother. (2007) 51:2988-2990.


pagina 28 di 31

31. Garcia-Saez I., Docquier J.D., Rossolini G.M., Dideberg O. The three-dimensional

structure of VIM-2, a Zn-β-lactamase from Pseudomonas aeruginosa in its reduced

and oxidised form. J. Mol. Biol. (2008) 375:604-611.

32. Cagnacci S., Gualco L., Roveta S., Mannelli S., Borgianni L., Docquier J.D., Dodi

F., Centanaro M., Debbia E., Marchese A., Rossolini G.M. Bloodstream infections

caused by multidrug-resistant Klebsiella pneumoniae producing the carbapenem-

hydrolysing VIM-1 metallo-β-lactamase: first Italian outbreak. J. Antimicrob.

Chemother. (2008) 61:296-300.

33. Michaux C., Massant J., Kerff F., Frère J.M., Docquier J.D., Vandenberghe I.,

Samyn B., Pierrard A., Feller G., Charlier P., Van Beeumen J., Wouters J. Crystal

structure of a cold-adapted class C β-lactamase. FEBS J. (2008) 275:1687-1697.

34. Gori Savellini G., Di Genova G., Terrosi C., Di Bonito P., Giorgi C., Valentini M.,

Docquier J.D., Cusi M.G. Immunization with Toscana virus N-Gc proteins

protects mice against virus challenge. Virology (2008) 375:521-528.

35. Stoczko M., Frère J.M., Rossolini G.M., Docquier J.D. Functional diversity among

metallo-β-lactamases: characterization of the CAR-1 enzyme of Erwinia carotovora.

Antimicrob. Agents Chemother. (2008) 52:2473-2479.

36. Docquier J.D., Calderone V., De Luca F., Benvenuti M., Giuliani F., Bellucci L.,

Tafi A., Nordmann P., Botta M., Rossolini G.M., Mangani S. The crystal structure

of the OXA-48 β-lactamase reveals mechanistic diversity among class D

carbapenemases. Chem. Biol. (2009) 16:540-547.

37. Zeba B., De Luca F., Dubus A., Delmarcelle M., Simporé J., Nacoulma O.J.,

Rossolini G.M., Frère J.M., Docquier J.D. Characterization of IND-6, a highly

divergent IND-type metallo-β-lactamase from Chryseobacterium indologenes strain

597 isolated in Burkina Faso. Antimicrob. Agents Chemother. (2009) 53:4320-4326.

38. Poirel L., Docquier J.D., De Luca F., Verlinde A., Ide L., Rossolini G.M.,

Nordmann P. BEL-2, an expanded-spectrum β-lactamase with increased activity

toward expanded cephalosporins in Pseudomonas aeruginosa. Antimicrob. Agents

Chemother. (2010) 54:533-535.

39. De Luca F., Giraud-Morin C., Rossolini G.M., Docquier J.D., Fosse T. Genetic and

biochemical characterization of TRU-1, the endogenous class C β-lactamase from

Aeromonas enteropelogenes. Antimicrob. Agents Chemother. (2010) 54:1547-1554.

40. Docquier J.D., Benvenuti M., Calderone V., Giuliani F., Kapetis D., De Luca F.,

Rossolini G.M., Mangani S. Crystal structure of the narrow-spectrum OXA-46

class D β-lactamase: relationship between active site lysine carbamylation and

inhibition by polycarboxylates. Antimicrob. Agents Chemother. (2010) 54:2167-74.

41. Borgianni L., Vandenameele J., Matagne A., Bini L., Bonomo R.A., Frère J.M.,

Rossolini G.M., Docquier J.D. Mutational analysis of VIM-2 reveals an essential


pagina 29 di 31

determinant for metallo-β-lactamase stability and folding. Antimicrob. Agents

Chemother. (2010) 54:3197-3204.

42. Lee K., Kim C.K., Hong S.G., Choi J., Song S., Koh E., Yong D., Jeong S.H., Yum

J.H., Docquier J.D., Rossolini G.M., Chong Y. Characteristics of clinical isolates of

Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases. Int. J.

Antimicrob. Agents (2010) 36:259-63.

43. Docquier J.D., Benvenuti M., Calderone V., Stoczko M., Menciassi N., Rossolini

G.M., Mangani S. High-resolution crystal structure of the subclass B3 BJP-1

metallo-β-lactamase: rational basis for substrate specificity and interaction with

sulfonamides. Antimicrob. Agents Chemother. (2010) 54:4343-4351.

44. Lee K., Kim C.K., Yong D., Jeong S.H., Yum J.H., Seo Y.H., Docquier J.D., Chong

Y. Improved performance of the modified Hodge test with MacConkey agar for

screening carbapenemase-producing Gram-negative bacilli. J. Microbiol. Methods

(2010) 83:149-152.

45. Santella G., Pollini S., Docquier J.D., Mereuta A. I., Gutkind G., Rossolini G.M.,

Radice M. Intercontinental dissemination of IMP-13-producing Pseudomonas

aeruginosa belonging in Sequence Type 621. J. Clin. Microbiol. (2010) 48:4342-4343.

46. Borgianni L., Prandi S., Salden L., Santella G., Hanson N.D., Rossolini G.M.,

Docquier J.D. Genetic context and biochemical characterization of the IMP-18

metallo-β-lactamase identified in a Pseudomonas aeruginosa isolate from the United

States. Antimicrob. Agents Chemother. (2011) 55:140-145.

47. Santella G., Docquier J.D., Gutkind G., Rossolini G.M., Radice M. Purification

and biochemical characterization of IMP-13 metallo-β-lactamase. Antimicrob.


48. Lassaux P., Traoré A.D.K., Loisel E., Favier A., Docquier J.D., Sohier J.S., Laurent

C., Bebrone C., Frère J.M., Ferrer J.L., Galleni M. Structural and biochemical

characterization of the subclass B1 metallo-β-lactamase VIM-4. Antimicrob.


49. Docquier J.D., Benvenuti M., Calderone V., Rossolini G.M., Mangani S. The

crystal structure of the extended spectrum β-lactamase TEM-72 inhibited by

citrate. Acta Crystallogr. F Struct. Biol. Cryst. Commun. (2011) F67:303-306.

50. Lee K., Yum J.H., Yong D., Jeong S.H., Rossolini G.M., Docquier J.D., Chong Y.

Biochemical characterization of the TEM-107 extended-spectrum β-lactamase in a

Klebsiella pneumoniae isolate from Korea. Antimicrob. Agents Chemother. (2011)

55:5930-5932.

51. De Luca F., Benvenuti M., Carboni F., Pozzi C., Rossolini G.M., Mangani S.,

Docquier J.D. Evolution to carbapenem-hydrolyzing activity in

noncarbapenemase class D β-lactamase OXA-10 by rational protein design. Proc.

Natl. Acad. Sci. U S A. (2011) 108:18424-18429.


pagina 30 di 31

52. Gualdi L., Kaur Hayre J., Gerlini A., Bidossi A., Colomba L., Trappetti C., Pozzi

G., Docquier J.D., Andrew P.W., Ricci S., Oggioni M.R.. Regulation of

neuraminidase expression in Streptococcus pneumoniae. BMC Microbiol. (2012)

12:200.

53. Kaur Hayre J., Xu G., Borgianni L., Taylor G.L., Andrew P.W., Docquier J.D.,

Oggioni M.R. Optimization of a direct spectrophotometric method to investigate

the kinetics and inhibition of sialidases. BMC Biochem. (2012) 13:19.

54. Pollini S., Maradei S., Pecile P., Olivo G., Luzzaro F., Docquier, J.D., Rossolini

G.M. FIM-1, a new acquired metallo-β-lactamase from a Pseudomonas

aeruginosa clinical isolate from Italy. Antimicrob. Agents Chemother. (2013)

57:410-416.

55. Lahiri S., Mangani S., Durand-Reville T., Benvenuti M., De Luca F., Sanyal G.,

Docquier J.D. Structural insight into potent broad-spectrum inhibition with

reversible recyclization mechanism: Avibactam in complex with CTX-M-15 and

Pseudomonas aeruginosa AmpC β-lactamases. Antimicrob. Agents Chemother.

(2013) 57:2496-2505.


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Capitoli di libri scientifici

1. Rossolini G.M., Docquier J.D. Class B -lactamases. p. 115-144. In “Resistance to

Antibiotics : Enzyme-Mediated Mechanisms and Prospects for Inhibition”, by M.

Tolmasky et R.A. Bonomo, editors. ASM Press, Washington, U.S.A. (2007).

2. Docquier J.D., Rossolini G.M., Nikaido H., Frère J.M. Interplay between beta-

lactamase activity, outer membrane and active efflux system. Chapter 16. In “β-

lactamases”, by J.M. Frère, editor. Nova Science Publishers, Hauppauge, USA

(2011).

Documents

Curriculum Vitae Jean-Denis Docquier · 2013-06-05 · Curriculum Vitae – Jean-Denis Docquier pagina 3 di 31 Institut di Biologie Structurale "Jean-Pierre Ebel" (Pr. Otto Dideberg),