CURRICULUM VITAE MINGEOT Marie-Paule, épouse Leclercq.

DONNEES PERSONNELLES: Naissance : 3 août 1957, Jemappes (Belgique) Statut : belge, mariée, 3 enfants ADRESSE PRIVEE: 64, Avenue Vander Biest 1150 Bruxelles tél: 02/7630862 ADRESSE PROFESSIONNELLE: Université Catholique de Louvain Faculté de Médecine - Ecole de Pharmacie Unité de Pharmacologie Cellulaire et Moléculaire 73, Avenue Emmanuel Mounier Bt7370 1200 Bruxelles tél: 02/7647374 fax: 02/7647373 courrier électronique: mingeot@facm.ucl.ac.be ETUDES ET DIPLOMES UNIVERSITAIRES Licence en Sciences Chimiques

1975-1979 (Grande Distinction) Agrégation de l'enseignement moyen supérieur en Sciences Chimiques

Septembre 1979 (Grande Distinction) Pharmacie

1979-1982 (Distinction) Agrégation de l'enseignement moyen supérieur en Pharmacie

Juin 1982 (Grande Distinction) Doctorat en Sciences Pharmaceutiques

Août 1984- mars 1988 (La Plus Grande Distinction et les félicitations du jury). Agrégation de l'enseignement supérieur

Juillet 2000 EXPERIENCE PROFESSIONNELLE ET STATUT 1982-1984 Université de Mostaganem (Algérie) - Faculté de Médecine - Chargée de l'enseignement des cours de Biochimie et de Pharmacologie - Chargée des travaux dirigés de Chimie organique - Chargée des travaux pratiques de microbiologie - Travaux de recherches portant sur la pollution chimique et microbiologique des eaux de la baie de Mostaganem.

1984- Université Catholique de Louvain (Belgique) - Chercheur sous contrats (1984-1987). - Chargé de Recherche FNRS (1987-1991). - Chercheur Qualifié du FNRS (1991-2001). - Maître de Recherches FNRS (2001-2002). - Chargé de cours invité - UCL (1994-1997). - Chargé de cours à temps partiel - UCL (1997-2002). - Chargé de cours à temps plein - UCL (2002-2003). - Professeur à temps plein � UCL (2003 �XXXX).

ACTIVITES DE RECHERCHE Thème principal de recherche : étude des interactions médicaments/membrane Publications (cf annexe) Reviews: 6 Book chapters: 4 Papers published in refereed scientific journals: 53 Abstracts published in proceedings of conferences: 112 Expertise aupres de firmes pharmaceutiques Bayer (Wuppertal) Combinatur Biopharm AG (Berlin) Collaborations scientifiques

- Université catholique de Louvain-UCL: o Unité de Biologie cellulaire (CELL): Prof. PJ Courtoy o Unité de Chimie des interfaces (CIFA) : Prof Y. Dufrêne o Unité de Chimie structurale et des mécanismes réactionnels (CSTR): Prof A.

Schanck o Unité de Chimie analytique, analyse des médicaments et pharmacognosie (CHAM):

Professeur J. Quétin-Leclercq o Unité de pharmacie galénique, industrielle et officinale (FARG): Professeur V. Préat

- Facultés agronomiques de Gembloux: o Centre de Biophysique moléculaire numérique (Prof. R. Brasseur) o Unité de Chimie biologique industrielle (Dr M. Deleu)

- Université libre de Bruxelles � ULB Unité de Structure et fonction des membranes biologiques (SFMB): Prof. E. Goormaghtigh

- Katholieke Universiteit Leuven

Laboratorium voor Farmaceutische Chemie, Rega Instituut (Prof. P.J. Claes, Prof. H.J. Vanderhaeghe, Dr A. Van Schepdael)

- School of Health and Caring professions, Technological Educational institutions of Athens, Greece (Dr E. Charvalos)

- Laboratory of Toxicology and Clinical Pathology, Department of Medicine, University of Crete, Greece (Prof M.M. Tzatzarakis, Prof. A.M. Tsatsakis et Prof. G.N. Tzanakakis)

- Agricultural University of Athens, Greece (Prof MP Gergiadis, Prof. V. Constantinou-Kokotou et Dr. S. Kotretsou)

- Center of molecular immunology, La Havane, Cuba (Prof. R. Perez) Financements actuels 1998- Industrie

UCB Etude de l'influence du piracetam sur la déstabilisation de phospholipides d'un modèle de membrane par le peptide de la protéine amyloïde - Etudes biophysiques

M.P. Mingeot

2000- Industrie Cheminova

Negative staining and electron microscopy photos of liposome formulations

M.P. Mingeot P. Courtoy

2002-2005 FSR Transport of peptides and protein across membranes: mechanisms of transport and optimisation.

O. Feron M.P. Mingeot V. Préat Y.J. Schneider P. Tulkens F.van BambekeR. Van Bever

2002-2006 FRSM Etude des interactions entre des molécules d�intérêt pharmacologique et les lipides. Caractérisation effets sur les propriétés biophysiques des mem- branes et conséquences au niveau cellulaire (endocytose, apoptose).

M.P. Mingeot Y. Dufrêne R. Brasseur

2002-2007 Région Wallonne

Développement d�un modèle membranaire nano- biomimétique à usage pharmacologique

Y. Dufrêne M.P. Mingeot P. Courtoy M. Paquot R. Brasseur E.Goormagtigh

2003-2005 FNRS Télévie

Etude des mécanismes impliqués dans le passage transmembranaire d'antibiotiques (fluoroquinolones) et depeptides (ghréline et désoctanoyl-ghréline). Implications d'un point de vue biophysique et pharmacologique

M.P Mingeot

2006-2010 FRSC Fabrication et caractérisation à l�échelle nanométrique de membrane biomimétiques : application à l�étude des interactions entre médicaments et membranes

Y. Dufrêne M.P. Mingeot

Encadrement de travaux de recherche de troisième cycle

- Promoteur ou copromoteur de 5 thèses de Docteur en Sciences Pharmaceutiques - Promoteur ou copromoteur de 6 mémoires de DEA en Sciences pharmaceutiques hors

Ecole doctorale) - Accueil de 3 chercheurs étrangers en séjour sabbatique ou postdoctorants - Membre du jury de 29 thèses (25 UCL et 4 hors UCL) en vue de l'obtention du grade de

Ph D - Membre du Comité d�encadrement de 17 thèses de doctorat en cours

Participation à des congrès nationaux et internationaux (1 ou 2 par an) Participation aux Commissions de Recherche de l�Ecole de Pharmacie

- Comité de gestion du DEA en Sciences pharmaceutiques: Présidente depuis 1995 - Commission d'étudiants chercheurs: Secrétaire de 1991-1995

Présidente depuis 1995. - Ecole Doctorale en Sciences pharmaceutiques: membre depuis 1999.

ENSEIGNEMENT

Cours assurés en 2005-2006 (62.5-72.5)

- Biochimie et biologie moléculaire (FARM 1221) (25-12.5) - Biophysique appliquée aux médicaments (FARM 1219) (10-5) - Pharmacologie spéciale (FARM 2146) (10-0) - Pharmacochimie et pharmacologie des nouveaux médicaments (FARM 2220) (10-5) - Séminaire intégré de Sciences pharmaceutiques (FARM 2301) (0-40) - Pharmacologie moléculaire (FARM 3346) (2.5-5) - Notions approfondies de physiopathologie, de biologie cellulaire et de biophysique en

rapport avec le médicament (FARM3502) (5-5) Promoteur ou copromoteur de 18 mémoires de second cycle Accueil de 3 stagiaires Eramus pour des durées égales ou supérieures à 6 mois Lecteur d�une vingtaine de mémoire de second cycle Participation à la Commission d�enseignement de l�Ecole de Pharmacie Secrétaire de 1992 à 1997. Membre de 2003- Initiatives et projets pédagogiques 1998-1999: Traduction française de la 3°° édition anglaise du livre de Neal �Pharmacology at a glance� (revision scientifique) et mise à disposition du livre aux étudiants. 2001-2002: Projet FDP (Promoteurs: V. Préat, P.M. Tulkens et M.P. Mingeot-Leclercq �Apprentissage de la Communication scientifique orale� 2003-2004: Traduction française de la 4° édition anglaise du livre de Neal �Pharmacology at a glance� (revision scientifique) et mise à disposition du livre aux étudiants. Conseiller d�ouvrages scientifiques Conseiller Scientifique chez De Boeck Université (Demandes d'avis + Revision scientifique de la traduction de la 3° et 4° édition anglaise du livre: M. Neal, En bref, Pharmacologie médicale, De Boeck Université)

IMPLICATION DANS LE FONCTIONNEMENT DE L�INSTITUTION Membre du CORA: Suppléant (1998-2000) et effectif (2000-2006) Membre du Bureau de l�Ecole de pharmacie: 1999- Membre du groupe de Réflexion de l�Ecole de Pharmacie: 2001- Secrétaire académique de l�Ecole de Pharmacie: 2002-2006 Chef d�Unité (FACM : Unité de pharmacologie cellulaire et moléculaire) : 2003-2008

ANNEXE Marie-Paule MINGEOT-LECLERCQ List of publications Janvier 2006 Contents

1. Reviews 2. Book chapters 3. Ph. D and aggregation thesis 4. Papers published in refereed international scientific journals 5. Papers published in proceeding of conferences

Reviews [R.6] Carryn S., H. Chanteux, C. Seral, M.-P. Mingeot-Leclercq, F. Van Bambeke & P.M. Tulkens. 2003. Intracellular Pharmacodynamics of Antibiotics. Infect. Dis. Clin. N. Am. 17: 615-634. (I.F. 1.781) [R.5] Mingeot-Leclercq, M.P. & P.M. Tulkens. 1999. Aminoglycosides: Nephrotoxicity. Antimicrob. Agents Chemother. 43:1003-1012 (I.F.1998: 3.761). [R.4] Mingeot-Leclercq, M.P., Y. Glupczynski & P.M. Tulkens. 1999. Aminoglycosides: Activity and resistance. Antimicrob. Agents Chemother. 43:727-737 (I.F. 1998: 3.761). [R.3] Mingeot-Leclercq, M.-P., A. Schanck, F. Van Bambeke, P.M. Tulkens, L. Lins & R. Brasseur. 1995. Molecular description of the interactions of aminoglycoside antibiotics with negatively-charged phospholipids. Theoretical molecular modelling and experimental results. Life Science/Advances in Pharmacology 14:71-98 (I.F. 1995: 1.148). [R.2] Mingeot-Leclercq, M.-P., R. Brasseur & A. Schanck. 1995. Molecular parameters involved in aminoglycosides nephrotoxicity. J. Toxicol. Environ. Health 44:263-300 (I.F. 1995: 1.417). [R1] Mingeot-Leclercq, M.P., G. Laurent, B.K. Kishore & P.M. Tulkens. 1991. Aminoglycosides nephrotoxicity. Life Sc. Adv. (Biochem. Pharmacol.) 10:113-141 (I.F. 1995: 1.148). Books chapters [B.4] Servais H., M.P. Mingeot-Leclercq & P.M. Tulkens. 2005. Antibiotic-induced nephrotoxicity. In Toxicology of the kidney. Ed. : J.B. Tarloff and L.H. Lash. Edit. CRC Press. Chapter 16: 635-685 [B.3] Van Bambeke, F., D. Lambert, M.P. Mingeot-Leclercq, & P. M. Tulkens. 2003. Anti-infective therapy: Mechanisms of action. In: Infectious Diseases. Ed. : J. Cohen et W.J. Powderly. Edit. Mosby, London, UK.

[B.2] Van Bambeke, F., D. Lambert, M.P. Mingeot-Leclercq & P.M. Tulkens. 1999. Antiinfective therapy: Mechanisms of action. In Infectious Diseases vol.section 7. Ed. D. Armstrong and J. Cohen, edit., Mosby, London, United Kingdom. 1.1-1.14. [B.1] Tulkens, P.M., M.P. Mingeot-Leclercq, G. Laurent & R. Brasseur. 1990. Conformational and biochemical analysis of the interactions phospholipids-aminoglycoside antibiotics in relation with their toxicity. In Molecular Description of Biological Membrane Components by Computer-aided Conformational Analysis vol.II. R. Brasseur, edit., CRC Press, Boca Raton, Fla. 63-93. Thesis [T.2] Mingeot-Leclercq, M.P.. 2000. �Biochemical and biophysical studies of the interactions beween aminoglycoside antibiotics and lipid layers: a molecular approach to the understanding of the cellular toxcicity induced by aminoglycosides�. Thèse d�agrégation pour le grade d�agrégé de l�enseignement supérieur. pp 163. [T.1] Mingeot-Leclercq, M.P. 1988. Mécanisme moléculaire de la néphrotoxicité induite par les aminoglycosides: études biochimiques et biophysiques. Thèse de Doctorat en Sciences Pharmaceutiques (Université Catholique de Louvain). pp 141. Papers published in referred international scientific journals [P.53] Servais H., Y. Jossin, F. Van Bambeke, P.M. Tulkens & M.-P. Mingeot-Leclercq (2006). Gentamicin causes apoptosis at low concentrations in renal LLC-PK1 cells subjected to electroporation. Antimicrob. Agents Chemother. In press. [P.52] Barcia-Macay M., C. Seral, M.-P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke (2006). Pharmacodynamic evaluation of the intracellular activity of antibiotics against Staphylococcus aureus in a model of THP-1 macrophages. Antimicrob. Agents Chemother. [P.51] Charvalos E., M.N. Tzatzarakis, F. Van Bambeke, P.M. Tulkens, A. M. Tsatsakis, G.N. Tzanakakis, & M.-P. Mingeot-Leclercq (2006). Water-soluble amphotericin B-polyvinylpyrrolidone complexes with maintained antifungal activity against Candida spp. and Aspergillus spp. and reduced haemolytic and cytotoxic effects. J. Antimicrob. Chemother. 57:236-44 [P.50] Berquand A., N. Fa, Y.F. Dufrêne & M.-P. Mingeot-Leclercq (2005). Interaction of the macrolide antibiotic, azithromycin, with lipid bilayers: effect on membrane organisation, fluidity and permeability. Pharm Res. 22: 465-474 (I.F. 2004: 2.940) [P.49] Block S., P. Gerkens, O. Peulen, O. Jolois, M.P. Mingeot-Leclercq, M.Cl. De Pauw-Gillet & J. Quetin-Leclercq (2005). Induction of apoptosis in Human promyelocytic leukemia cells by a natural trachylobane diterpene. Anticancer Res. 25: 363-368 (I.F. 2004: 1.395) [P.48] Chanteux H., F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens (2005). Accumulation and oriented transport of ampicillin in Caco-2 cells from its pivaloyloxymethylester prodrug of ampicillin (pivampicillin). Antimicrob. Agents Chemother. 49: 1279-1288 (I.F. 2004: 4.216) [P.47] Lemaire S., F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tukens (2005). Activity of three β-lactams (ertapenem, meropenem and ampicillin) against intraphagocytic Listeria monocytogenes and Staphylococcus aureus. J. Antimicrob. Chemother. 55: 897-904 (I.F. 2004: 3.611)

[P.46] Michot J.-M., C. Seral, F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens (2005). Influence of efflux transporters on the accumulation and efflux of four quinolones (ciprofloxacin, levofloxacin, garenofloxacin, and moxifloxacin) in J774 macrophages. Antimicrob. Agents Chemother. 49: 2429-2437 (I.F. 2004: 4.216) [P.45] Piret J., A. Schanck, S. Delfosse, F. Van Bambeke, B.K. Kishore, P.M. Tulkens & M.-P. Mingeot-Leclercq (2005). Modulation of the In vitro activity of lysosomal phospholipase A& by membrane lipids. Chem. Phys. Lipids. 133: 1-15 (I.F. 2004: 1.971) [P.44] Seral C., M. Barcia-Makay, M.-P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke (2005). Comparative activity of quinolones (ciprofloxacin, levofloxacin, moxifloxacin, and garenofloxacin) against extracellular and intracellular infection by Listeria monocytogenes and Staphylococcus aureus in J774 macrophages. J. Antimicrob. Chemother. 55: 511-517 (I.F. 2004: 3.611) [P.43] Servais H., P. Van Der Smissen, G. Thirion, G. Van der Essen, F. Van Bambeke, P.M. Tulkens & M.-P. Mingeot-Leclercq (2005). Gentamicin-induced apoptosis in LLC-PK1 cells: involvement of lysosomes and mitochondrial. Toxicol. Appl. Pharmacol. 206 : 321-333 (I.F. 2004: 2.618) [P.42] Van Bambeke F., J. Saffran, M.-P. Mingeot-Leclercq & P.M. Tulkens (2005). Mixed lipid storage disorder induced in macrophages and fibroblasts by oritavancin (LY333328), a new glycopeptide antibiotic with exceptional cellular accumulation. Antimicrob. Agents Chemother. 49: 1695-1700 (I.F. 2003: 4.216) [P.41] Ben Abdelaziz H., S. Lemaire, S. Carryn, F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens (2004). Inhibition of TNF-α production in THP-1 macrophages by glatiramer acetate does not alter their susceptibility to infection by Listeria monocytogenes and does not impair the efficacy of ampicillin or moxifloxacin against intracellular bacteria. J. Antimicrob. Chemother. 288-289. (I.F. 2002: 3.080). [P.40] Berquand A., M.-P. Mingeot-Leclercq & Y. Dufrêne. (2004). Real-time imaging of drug-membrane interactions by atomic force microscopy. Biochem. Biophys. Acta (Biomembranes). 1664: 198-205. (I.F. 2003: 2.665) [P.39] Carryn S., S. Vandevelde, F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens (2004). Impairment of Growth of Listeria monocytogenes in THP-1 Macrophages by Granulocyte-Macrophage Colony Stimulating Factor. Release of Tumor Necrosis Factor-α and Nitric Oxide. J.Inf.Dis. 189: 2101-2109 (I.F. 2003: 4.481). [P.38] Nassogne MC, Lizarraga C, N'Kuli F, Van Bambeke F, Van Binst R, Wallemacq P, Tulkens PM, Mingeot-Leclercq MP, Levade T, Courtoy PJ. (2004). Cocaine induces a mixed lysosomal lipidosis in cultured fibroblasts, by inactivation of acid sphingomyelinase and inhibition of phospholipase A1. Toxicol Appl Pharmacol. 194101-110 (I.F.2003: 2.851). [P.37] Michot J.-M., F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens. (2004). Active efflux of ciprofloxacin from J774 macrophages through MRP-like transporter. Antimicrob. Agents Chemother. 48: 2673-2682. (I.F. 2003: 4.246) [P.36] Van Bambeke F., S. Carryn, C. Seral, H. Chanteux, D. Tyteca, M.-P. Mingeot-Leclercq & P.M. Tulkens. (2004). Cellular pharmacokinetics and pharmacodynamics of the glycopeptide antibiotic oritavancin (LY333328) in a model of J774 mouse macrophages. Antimicrob. Agents Chemother. 48: 2853-2860. (I.F. 2003: 4.246)

[P.35] Carryn S., F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens. 2003. Activity of β-lactams (ampicillin, meropenem), gentamicin, azithromycin and moxifloxacin against intracellular Listeria monocytogenes in a 24 h THP-1 human macrophages model. J. Antimicrob. Chemother. 51: 1051-1052 (I.F. 2002: 3.329) [P.34] Chanteux H., M.-P. Mingeot-Leclercq, E. Sonveaux, F. Van Bambeke & P.M. Tulkens. (2003). Intracellular accumulation and activity of ampicillin used as free drug and as its phthalimidomethylester or pivaloyloxymethyl ester (pivampicillin) against Listeria monocytogenes in J774 macrophages. J. Antimicrob. Chemother. 52: 610-615 (I.F. 2002: 3.329). [P.33] Chanteux H., I. Paternotte, M.-P. Mingeot-Leclercq, R. Brasseur, E. Sonveaux & P.M. Tulkens. 2003. Cell handling, membrane-binding properties and membrane-penetration modeling approaches of pivampicillin and phthalimidomethylampicillin, two basic esters of ampicillin, in comparison with chloroquine and azithromycin. Pharm. Res. 20: 624-631 (I.F. 2002:2.354). [P.32] Dom G., C. Shaw-Jackson, C. Matis, O. Bouffioux, J. Picard, A. Prochiantz, M.-P. Mingeot-Leclercq, R. Brasseur and R. Rezsohazy. 2003. Cellular uptake of Antennapedia penetratin peptides is a two-step process in which phase transfer precedes a tryptophan-dependent translocation. Nucleic Ac. Res. 31: 556-561 (I.F. 2002:7.051). [P.31] Mingeot-Leclercq M.-P., L. Lins, M. Bensliman, A. Thomas, F. Van Bambeke, J. Peuvot, A. Schanck and R. Brasseur. 2003. Piracetam inhibits the lipid-destabilizing properties of the Aβ c-terminal fragment. Biochemica Biophysica Acta - Biomembranes. 1609:28-38 (I.F. 2002: 3.011). [P.30] Seral C., J.-M. Michot, H. Chanteux, M.-P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke. 2003. Influence of P-glycoprotein inhibitors on accumulation of macrolides in J774 murine macrophages. Antimicrob. Agents Chemother. 47: 1047-1051 (I.F. 2002:4.215). [P.29] Tyteca D., A. Schanck, Y. Dufrêne, M. Deleu, P.J. Courtoy, P.M. Tulkens & M.-P. Mingeot-Leclercq. 2003. The macrolide antibiotic azihromycin interacts with lipids and affects membrane organisation and fluidity: studies on Langmuir-Blodgett monolayers, liposomes and J774 macrophages. J. Memb. Biol. 192: 203-215 (I.F. 2002: 2.440). [P.28] Carryn S., F. Van Bambeke, M.-P. Mingeot-Leclercq & P.M. Tulkens. 2002. Comparative intracellular (THP-1 macrophages) and extracellular activities of β-lactams, azithromycin, gentamicin and fluoroquinolones against Listeria monocytogenes at clinically-relevant concentrations. Antimicrob. Agents Chemother. 46:2095-2103 (I.F. 2001: 4.562) [P.27] Mingeot-Leclercq M.-P., L. Lins, M. Bensliman, F. Van Bambeke, J. Peuvot, A. Schanck & R. Brasseur. 2002. Membrane destabilization induced by β-amyloid peptide 29-42: importance of the amino-terminus. Chem. Phys. Lipids. 120:57-74 (I.F. 2001: 2.164) [P.26] Tyteca D., P. Van Der Smissen, M. Mettlen, F. Van Bambeke, P.M. Tulkens, M.-P. Mingeot-Leclercq & P.J. Courtoy. 2002. Azithromycin, a lysosomotropic antibiotic, has distinct effects on fluid-phase and receptor-mediated endocytosis, but does not impair phagocytosis in J774 macrophages. Exp. Cell Res. 281:86-100 (I.F. 2001: 5.096) [P.25] Viaene E., H. Chanteux, H. Servais, M.-P. Mingeot-Leclercq & P.M. Tulkens. 2002. Comparative stability studies of antipseudomonal β-lactams for potential administration through portable elastomeric pumps (home therapy for cystic fibrosis patients) and motor-operated syringes (intensive care units). Antimicrob. Agents Chemother. 46:2327-2332 (I.F. 2001: 4.562)

[P.24] M.-P. Mingeot-Leclercq, X. Gallet, C. Flore, F. Van Bambeke, J. Peuvot & R. Brasseur. 2001 Experimental and conformational analyses of interactions between butenafine and lipids. Antimicrob. Agents Chemother. 45:3347-3354 (I.F. 2000: 3.954) [P.23] Tyteca D., P. Van Der Smissen, F. Van Bambeke, K. Leys, P.M. Tulkens, P.J. Courtoy, & M.-P. Mingeot-Leclercq. 2001. Azithromycin, a lysosomotropic antibiotic, impairs fluid-phase pinocytosis in cultured fibroblasts. Eur.J.Cell Biol. 80:466-478 (I.F.1999: 3.034). [P.22] El Mouedden M., Laurent G., M.-P. Mingeot-Leclercq & P.M. Tulkens. 2000. Apoptosis in renal proximal tubules of rats treated with low doses of aminoglycosides. Antimicrob. Agents Chemother. 44: 665-675 (I.F. 1999: 3.969). [P.21] El Mouedden M., G. Laurent, M.-P. Mingeot-Leclercq & P.M. Tulkens. 2000. Gentamicin-induced apoptosis in renal cell lines and embryonic rat fibroblasts. Toxicol. Sciences. 56:229-239 (I.F. 1999: 1.778). [P.20.] Tyteca D., F. Van Bambeke, P.M. Tulkens, & M.-P. Mingeot-Leclercq. 2000. Azithromycin, a lysosomotropic antibiotic impairs endocytosis in cultured macrophages. Chem.Phys. Lipids. 107:58-59 (I.F. 2000: 2.328). [P.19] Van Bambeke F., A. Kerkhofs, A. Schanck, C. Remacle, E. Sonveaux, P.M. Tulkens & M.P. Mingeot-Leclercq. 2000. Biophysical studies and intracellular destabilization of pH-sensitive liposomes. Lipids 35:213-223 (I.F. 1999: 2.004). [P.18] Montenez, J.P., F. Van Bambeke, J. Piret, R. Brasseur, P.M. Tulkens & M.P. Mingeot-Leclercq. 1999. Interactions of macrolide antibiotics (erythromycin A, roxithromycin, erythromycylamine and azithromycin) with phospholipids: comparative studies with cultured cells, a-cellular systems, and computer-aided conformational approaches. Toxicol. Appl. Pharm. 156:129-140 (I.F. 1998: 2.654). [P.17] Van Bambeke, F., J.P. Montenez, J. Piret, P.M. Tulkens, P.J. Courtoy & M.P. Mingeot-Leclercq. 1996. Interaction of the macrolide azithromycin with phospholipid membranes: I. Inhibi-tion of lysosomal phospholipase A1 activity. Eur. J. Pharmacol. 314:203-214 (I.F. 1996: 2.339). [P.16] Montenez, J.P., F. Van Bambeke, J. Piret, A. Schanck, P.M. Tulkens, R. Brasseur & M.P. Mingeot-Leclercq. 1996. Interaction of the macrolide azithromycin with phospholipids. II. Biophysical and computer-aided conformational studies. Eur. J. Pharmacol. 314:215-227 (I.F. 1996: 2.339). [P.15] Van Bambeke, F., M.-P., Mingeot-Leclercq, R. Brasseur, P.M. Tulkens & A. Schanck. 1996. Aminoglycoside antibiotics prevent the formation of non bilayer structures in negatively-charged membranes. Comparative studies using fusogenic [bis-(beta-diethylaminoethylether)hexestrol] and aggregating (spermine) agents. Chem. Phys. Lipids 79:123-135 (I.F. 1996: 2.118). [P.14] Kotretsou, S., M.-P. Mingeot-Leclercq, V. Constantinou-Kokotou, R. Brasseur, M.P. Georgiadis & P.M. Tulkens. 1995. Synthesis and antimicrobial and toxicological studies of amino acid and peptide derivatives of kanamycin A and netilmicin. J. Med. Chem. 38:4710-4719 (I.F. 1994: 4.453). [P.13] Van Bambeke, F., P.M. Tulkens, R. Brasseur & M.-P. Mingeot-Leclercq. 1995. Aminoglycoside antibiotics induce aggregation but not fusion of negatively-charged liposomes. Eur. J. Pharmacol. 289:321-333 (I.F. 1995: 2.637).

[P.12] Van Bambeke, F., M.P. Mingeot-Leclercq, A. Schanck, R. Brasseur & P.M. Tulkens. 1993. Alterations in membrane permeability induced by aminoglycoside antibiotics: studies on liposomes and cultured cells. Eur. J. Pharmacol. 247:155-168 (I.F. 1994: 2.677). [P.11] Mingeot-Leclercq, M.-P., P.M. Tulkens & R. Brasseur. 1992. Accessibility of aminoglycosides, isolated and in interaction with phosphatidylinositol, to water: a conformational analysis using the concept of molecular hydrophobicity potential. Biochem. Pharmacol. 44:1967-1975 (I.F. 1995: 2.447). [P.10] Schanck, A., M.P. Mingeot-Leclercq, P.M. Tulkens, D. Carrier, I.C.P. Smith & H.C. Jarrel. 1992. Interactions of aminoglycosides with phospholipids. A deuterium Nuclear Magnetic Resonance study. Chem. Phys. Lipids 62:153-163 (I.F. 1996: 2.118). [P.9] Schanck, A., R. Brasseur, M.P. Mingeot-Leclercq & P.M. Tulkens. 1992. Interactions of aminoglycosides with phosphatidylinositol: a 15N Nuclear Magnetic Resonance study. Magn. Res. Chem. 30:11-15 (I.F. 1995: 1.192). [P.8] Mingeot-Leclercq, M.P., A. Van Schepdael, R. Brasseur, R. Busson, H.J. Vanderhaeghe, P.J. Claes & P.M. Tulkens. 1991. New derivatives of Kanamycin B obtained by modifications and substitutions in position 6". II. In vitro and computer-aided toxicological evaluation, with respect to interactions with phosphatidylinositol. J. Med. Chem. 34:1476-1482 (I.F.1995: 4.453). [P.7] Van Schepdael, A., J. Delcourt, M. Mulier, R. Busson, M.P. Mingeot-Leclercq, P.M. Tulkens & P.J. Claes. 1991. New derivatives of kanamycin B obtained by modifications and substitutions in position 6". I. Synthesis and microbiological evaluation. J. Med. Chem. 34:1468-1475 (I.F. 1995: 4.453). [P.6] Van Schepdael, A., R. Busson, L. Verbist, H.J. Vanderhaeghe, M.P. Mingeot-Leclercq, R. Brasseur & P.M. Tulkens. 1991. Synthesis, antibacterial activity and toxicological evaluation (in vitro and computer-aided) of 1-C-hydroxymethyl kanamycin B and 1-C-hydroxymethyl, 6"chlorokanamycin B. J. Med. Chem. 34:1483-1492 (I.F. 1995: 4.453). [P.5] Mingeot-Leclercq, M.P., J. Piret, P.M. Tulkens & R. Brasseur. 1990. Effect of acidic phospholipids on the activity of lysosomal phospholipases and on their inhibition by aminoglycoside antibiotics. II. Conformational analysis. Biochem. Pharmacol. 40:499-506 (I.F. 1995: 2.447). [P.4] Mingeot-Leclercq, M.P., J. Piret, R. Brasseur & P.M. Tulkens. 1990. Effect of acidic phospholipids on the activity of lysosomal phospholipases and on their inhibition by aminoglycoside antibiotics. I. Biochemical analysis. Biochem. Pharmacol. 40:489-497 (I.F. 1995: 2.447). [P.3] Mingeot-Leclercq, M.P., A. Schanck, M.F. Ronveaux-Dupal, M. Deleers, R. Brasseur, J.M. Ruysschaert & P.M. Tulkens. 1989. Ultrastructural, physico-chemical and conformational study of the interactions of gentamicin and bis(beta-diethylaminoethylether)hexestrol with negatively charged phospholipid bilayers. Biochem. Pharmacol. 38:729-741 (I.F. 1995: 2.447). [P.2] Mingeot-Leclercq, M.P., G. Laurent & P.M. Tulkens. 1988. Biochemical mechanism of aminoglycoside-induced inhibition of phosphatidylcholine hydrolysis by lysosomal phospholipases. Biochem. Pharmacol. 37:591-599 (I.F. 1995: 2.447). [P.1] Carlier M.B., R. Brasseur, G. Laurent, M.P. Mingeot-Leclercq, J.M. Ruysschaert & P.M. Tulkens. 1986. Use of an in vitro model to assess aminoglycoside-phospholipid interactions in relation with their nephrotoxicity. Fd Chem. Toxicol. 24:815-816 (I.F. 1996: 1.398).

Published abstracts or papers published in proceedings of conferences [A.112] S. Lemaire, F. Van Bambeke, P.M. Tulkens, M.P. Mingeot-Leclercq, J. Coyette, and Y. Glupczynski. Meropenem and cloxacillin are active against MRSA clinical isolates (including VISA) in acidic broth and in THP-1 macrophages. 16th European Congress of Clinical Microbiology and Infectious diseases. Nice, France. [A.111] N. Mesaros, Y. Glupczynski, D. Pierard, A. Dediste, Y. Van Laethem, P.M. Tulkens, M.P. Mingeot-Leclercq, & F. Van Bambeke. Detection of mexA and mexX efflux genes in P. aeruginosa: correlation between QC-RT-PCR and Real-Time PCR. 16th European Congress of Clinical Microbiology and Infectious diseases. Nice, France. [A.110] M. Barcia-Macay, M.P. Mingeot-Leclercq, P.M. Tulkens, & F. Van Bambeke. Pharmacodynamic evaluation of the bactericidal activity of telavancin (TLV) against extracellular and intracellular MSSA and MRSA. 16th European Congress of Clinical Microbiology and Infectious diseases. Nice, France. [A.109] Barcia-Macay M., M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke Pharmacological comparison of antibiotics against intracellular S. aureus. 15th European Congress of Clinical Microbiology and Infectious diseases. Copenhagen, Denmark. O120. [A.108] Barcia-Macay M., M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke Pharmacological comparison of antibiotics against intracellular S. aureus. 15th European Congress of Clinical Microbiology and Infectious diseases. Copenhagen, Denmark [A.107] Barcia-Macay M., P.M. Tulkens & F. Van Bambeke. 2005. Telavancin (TLV) accumulates in cultured macrophages (MPH) and is active against intracellular S. aureus. In 45th Interscience Conference on Antimicrobial Agents and Chemotherapy. New Orleans, La. Abstract A1831. [A.106] Caceres N., M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke. 2005. Development of a protocol for extraction and preparation of macrophage integral membrane proteins for proteomics analysis by the conventional 2DE-MS approach. ABRF2005 � Biomolecular technologies. Savannah, GA. P139-S [A.105] Fa N., A. Berquand, Y.F. Dufrêne & M.P. Mingeot-Leclercq. 2005. Interaction of the macrolide antibiotic azithromycin with model of membranes. Biophysical Society Annual Meeting. Long Beach, CA. [A.104] Fa N., I. Burton, M. Deleu, R. Brasseur, Y.F. Dufrêne & M.P. Mingeot-Leclercq. 2005. Probing fluoroquinolone-biomembrane interactions on the nanoscale. Lipids, Liposomes and Biomembranes. Vancouver, Canada. [A.103] Heremans M., M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke. 2005. Ciprofloxacin (CIP) accumulation and intracellular activity against L. monocytogenes are reduced in ciprofloxacin-resistant macrophages due to increased efflux. 15th European Congress of Clinical Microbiology and Infectious diseases. Copenhagen, Denmark [A.102] Lemaire S., F. Van Bambeke, M.P. Mingeot-Leclercq, Y. Glupczynski & P.M. Tulkens. 2005. Carbapenems (ETP,MEM) areactive against intraphagocytic MRSA in a THP-1 macrophage model. In 45th Interscience Conference on Antimicrobial Agents and Chemotherapy. New Orleans, La. Abstract

[A.101] Mathot F., L. van Beijsterveldt, F. Van Bambeke, M.P. Mingeot-Leclercq, T. Arien, M. Brewster & V. Préat. 2005. [A.100] Mesaros N., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2005. Phenotypic and genotypic detection of 3 Mex efflux pumps in P. aeruginosa. 15th European Congress of Clinical Microbiology and Infectious diseases . Copenhagen, Denmark [A.99] Mesaros N., N. Caceres, F. Van Bambeke, M.P. Mingeot-Leclercq, Y. Glupczynski. & P.M. Tulkens. 2005. Genotypic method is more reliable than phenotypic characterization to detect Mex efflux pumps in clinical isolates of P. aeruginosa. In 45th Interscience Conference on Antimicrobial Agents and Chemotherapy. New Orleans, La. Abstract [A.98] Servais H., Y. Jossin, F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2005. Gentamicin (GEN) causes apoptosis (APO) at low concentrations in LLC-PK1 cells subjected to electroporation (EP). In 45th Interscience Conference on Antimicrobial Agents and Chemotherapy. New Orleans, La. Abstract [A.97] Barcia-Macay M., S. Carryn, S. Lemaire, C. Seral, M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke. 2004. Intracellular models of infection to evaluate antibiotic activity. Belgian Society for Microbiology. [A.96] Barcia-Macay, M., M.P. Mingeot-Leclercq, P.M. Tulkens, & F. Van Bambeke. 2004. Beta-Lactams are Bactericidal against Intracellular Forms of S. aureus in a 24 h Human Macrophages Model (THP-1 Cells). In 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC. Abstract A 1488. [A.95] Basma V., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. Stability and compatibility of temocillin for administration by continuous infusion in intensive care patients. In 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, Czech Republic. Abstract R 1919. [A.94] Basma V., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. Stability and compatibility of vancomycin for administration by continuous infusion in intensive care patients. In 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, Czech Republic. Abstract R 1920. [A.93] Charvalos E., M.N. Tzatzarakis, F. Van Bambeke, P.M. Tulkens, A.M. Tsatsakis & M.P. Mingeot-Leclercq. 2004. In vitro antifungal activity, cytotoxicity and cellular accumulation of new water soluble amphotericin B (AMB) � polyvinylpyrrolidone (PVP) formulations. 14th European Congress of Clinical Microbiology and Infectious diseases. Prague, Czech Republic. Abstract P 1785. [A.92] Fa N., A. Berquand, Y. Dufrêne & M.P. Mingeot-Leclercq. 2004. Interaction of the macrolide antibiotic azithromycin with model of membranes. 3rd Portugese-Spanish Biophysics Congress. Lisbon, Portugal. Oral communication � Abstract 009. [A.91] Heremans M., J.M. Michot, M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke. 2004. Ciprofloxacin-Resistant J774 Macrophages Show a Reduction in the Accumulation of Quinolones (Q) Through Increased Activity of an MRP-Like Transporter. In 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC. Abstract A 1304. [A.90] Fa N., A. Berquand & M.-P. Mingeot-Leclercq. 2004. Effet de l�azithromycin, un antibiotique de la classe sur des membranes modèles. 11° Congres GEIMM (Groupe d�Etudes des Interactions Molecules Membranes). Dinard, France. Abstract O10.

[A.89] Lemaire S., S. Van de Velde, S. Carryn, F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. Comparison of ertapenem, ampicillin, and meropenem against the intracellular forms of Listeria monocytogenes in human THP-1 macrophages. In 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, Czech Republic. Abstract P 1045. [A.88] Lemaire S., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. Contrasting Effects of Ertapenem (ETP) against intracellular L. monocytogenes (L.m.) and S. aureus (S.a.) in a model of THP-1 macrophages. In 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC. Abstract A 1487. [A.87] Mesaros N., N. Caceres, F. Van Bambeke, F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. Quantification of mexAB-oprM and mexXY-oprM gene expression level in Pseudomonas aeruginosa by QT-RT-PCR. Belgian Society for Microbiology. [A.86] Mesaros N., N. Caceres, F. Van Bambeke, F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. A rapid and versatile method to quantify the expression level of mexAB-oprM and mexXY-oprM genes in Pseudomonas aeruginosa. ECC and RICAI 2004 Congress. 564/91P [A.85] M.-P. Mingeot-Leclercq. (2004). Drug � Membrane interactions: analysis, drug distribution, Modelling. In Book reviews. Chem Bio Chem. 5:387-388. [A.84] Servais H., E. Delbecq, G. Van der Essen, F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 2004 Involvement of Mitochondrial Pathway and Bax in gentamicin-induced Apoptosis in renal LLC-PK1 Cells. Peptide-membrane Interaction, Namur. [A.83] Servais H., E. Delbecq, G. Van der Essen, G. Thirion, F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 2004 Involvement of Mitochondrial Pathway and Bax in Gentamicin (GEN)-induced Apoptosis in renal LLC-PK1 Cells. In 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC. Abstract A 1491. [A.82] Staes E., M.P. Mingeot-Leclercq & V. Préat. 2004. Ghrelin acylation strongly influences its binding to albumin and to lipid bilayers. Peptide-membrane Interaction, Namur. [A.81] Van de Velde S., S. Carryn, F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2004. PMA activation increases accumulation and activity against intracellular Listeria monocytogenes in human THP-1 macrophages. ISOPOL XV. [A.80] Barcia-Macay C., C. Seral, M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke F., 2003. Comparative activity of 12 antibiotics (ABs) used at clinically-meaningful extracellular concentration against S. aureus in broth and in a model of human THP-1 macrophages (M). In 43th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no.2357 [A.79] Carryn S., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2003. GM-CSF limits the intracellular growth of Listeria monocytogenes in THP-1 human macrophages. 13th European Congress of Clinical Microbiology and Infectious Diseases, Glasgow, UK. Abstract no.0153. [A.78] Chanteux H., M.-P. Mingeot-Leclercq, F. Van Bambeke & P.M. Tulkens. 2003. Pivampicillin enhances the activity of ampicillin against intracellular bacteria: studies in a model of J774 macrophages infected with Listeria monocytogenes, 13th European Congress of Clinical Microbiology and Infectious Diseases, Glasgow, UK. Abstract no.0393

[A.77] Seral C., E. Viaene, F. Van Bambeke, M.P Mingeot-Leclercq & P.M. Tulkens. 2003. Influence of inhibitors of efflux pumps on the accumulation of quinolones in a model of Calu-3 human airways epithelial cells. In 43th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no.2329 [A.76] Servais H., P. Van Der Smissen, G. Thirion, G. Van der Essen, P. Jacquemin, F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 2003. Molecular pathway(s) involved in gentamicin-induced apoptosis in a renal cell line: role played by lysosomes? In Apoptosis 2003. From signaling pathways to therapeutic tools. Luxembourg, Luxembourg. Abstract V.50. [A.75] Van Bambeke F., H. Chanteux, D. Tyteca, M.P. Mingeot-Leclercq & P.M. Tulkens. 2003. Oritavancine accumulates to high levels in the lysosomes of macrophages by endocytosis. In 43th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no.2063 [A.74] Carryn S., F. J. Zanon, Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2002. β-Lactams like fluoroquinolones are bactericidal against intracellular (THP-1 macrophages) Listeria monocytogenes after 24h. In 17th symposium de la société belge d�infectiologie et de microbiologie clinique, Bruxelles, Belgium. [A.73] Chanteux H., F. Van Bambeke, M.P. Mingeot-Leclercq, E. Sonveaux & P.M. Tulkens. 2002. Cellular accumulation and membrane-binding properties of ester prodrugs of ampicillin (pivaloylampicillin, phthalimidomethylampicillin). In 12th European Congress of Clinical Microbiology and Infectious Diseases. Milan, Italy. Abstract P 1349. [A.72] Chanteux H., J. Michot, C. Seral, F. Van Bambeke, M.P. Mingeot-Leclercq, P. M. Tulkens. 2002. Ampicillin [AMPI] and Ester Prodrugs of AMPI are not Substrates of MRP and P-Gp in J774 Macrophages In 42nd Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego, Cal. A 488 [A.71] Chanteux H., F. Van Bambeke, M.P. Mingeot-Leclercq, E. Sonveaux & P.M. Tulkens. 2002. Oriented Transport of Ampicillin Through Caco-2 Intestinal Cells Exposed to Pivaloyl and Phtalimidomethyl Esters of Ampicillin. In 42th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego, Cal. Abstract no. A 487. [A.70] Michot J-M. F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2002. Development of a Ciprofloxacin-Resistant J774 Macrophage (MØ) Clone [RS80] and Characterization of Ciprofloxacin (CIP) and Moxifloxacin (MXF) Accumulation. In 42th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego, Cal. Abstract no.A 486. [A.69] Seral C., J.-M. Michot, H. Chanteux, M.P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke,. 2002. Inhibitors of P-glycoprotein increase the accumulation of azithromycin and other macrolides in macrophages. In 12th European Congress of Clinical Microbiology and Infectious Diseases. Milan, Italy. Abstract P 1385. [A.68] Servais H., P.M. Tulkens & M.P. Mingeot-Leclercq. 2002. Detection of apoptosis induced by gentamicin in a renal cell line (LLC-PK1 cells). Cell signaling transcription and translation as therapeutic targets. Luxembourg. Abstract n°IX.14. [A.67] Van Bambeke F., J. Saffran, M.P. Mingeot-Leclercq & P.M. Tulkens. 2002. Oritavancin Glycopeptide Causes a Lipid Storage Disorder and Mixed Morphological Alterations of the Vacuolar System in Cultured Rat Embryo Fibroblasts (FB) and J774 Mouse Macrophages (MΦ). In 42th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego. Abstract no. A 508.

[A.66] Carryn S., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2001. Pharmacodynamics of two beta-lactams and two fluoroquinolones against extracellular and intracellular Listeria monocytogenes. In 41th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no. A 2099. [A.65] Carryn S., F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2001. Pharmacodynamics of two beta-lactams and two fluoroquinolones against extracellular and intracellular Listeria monocytogenes. In 9th ISAP International symposium PK/PD at unusual site of infection, Chicago, Ill. [A.64] Chanteux H., F. Van Bambeke, M.P. Mingeot-Leclercq, E. Sonveaux & P.M. Tulkens. 2001. Pharmacological evaluation of an ester prodrug of ampicillin tailored for the treatment of intracellular infection in comparison with pivaloylampicillin. In 10th Forum of Pharmaceutical Sciences of the Belgian Society of Pharmaceutical Sciences. Montreal, Canada. (Oral communication). [A.63] Chanteux H., F. Van Bambeke, M.P. Mingeot-Leclercq, E. Sonveaux & P.M. Tulkens. 2001. Accumulation of ester prodrugs of ampicilin (AMPI) in J774 macrophages: mechanism and activity against intracellular Listeria monocytogenes. In 41th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no. A 2070. [A.62] J.M. Michot, M.P. Mingeot-Leclercq, F Van Bambeke & P.M. Tulkens. 2001. Ciprofloxacin (CIP), but not moxifloxacin (MOX), is a substrate of both MRP1 and MDR1 transporters in murine J774 Macriophages. In 41th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no. A 2208. [A.61] Mingeot-Leclercq M.P., X. Gallet, F. Van Bambeke, C. Flore, J. Peuvot & R. Brasseur. 2001. Experimental and conformational analysis of the interactions between the antifungal butenafine and lipids. In 11th European Congress of Clinical Microbiology and Infectious Diseases, Istanbul, Turkey. Abstract P427. [A.60] Tyteca D., F. Van Bambeke, P.M. Tulkens, P.J. Courtoy & M.P. Mingeot-Leclercq. 2001. O Azithromycin (AZ), a lysosomotropic antibiotic, differently impairs various types of endocytosis in cultured macrophages. In 673rd Biochemical Society Meetings, Bristol, United Kingdom. Abstract 85. [A.59] Van Bambeke F., A.S. Snoeck, H. Chanteux, M.P. Mingeot-Leclercq & PM. Tulkens. 2001. Is LY333328 glycopeptide a new cell-associated antibiotic? Comparative studies with vancomycin and azithromycin in a model of J774 mouse macrophages. In 11th European Congress of Clinical Microbiology and Infectious Diseases, Istanbul, Turkey. Abstract P1245. [A.58] Van Bambeke F., S. Carryn, A.S. Snoeck, M.P. Mingeot-Leclercq & P.M. Tulkens. 2001. LY333328 (Oritavancin) glycopeptide accumulates in cultured macrophages but is only bacteriostatic towards intracellular Listeria monocytogenes. In 41th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, Ill. Abstract no. A 2069. [A.57] Carryn S., Y. Ouadhriri, F. Van Bambeke, M.P. Mingeot-Leclercq & P.M. Tulkens. 2000. Pharmacodynamics of two β-lactams (ampicillin [AMPI] and meropenem [MERO]) and 2 fluoroquinolones (sparfloxacin [SPAR] and moxifloxacin (MOXI)] against extracellular and intracellular Listeria monocytogenes. In 15th symposium de la société belge d�infectiologie et de microbiologie clinique, Bruxelles, Belgium. [A.56] Michot J.-M., F. Van Bambeke, M.-P. Mingeot & P.M. Tulkens. 2000. Identification and characterization of a ciprofloxacin (CPFX) efflux transporter in murine J774 macrophages. In 40th Interscience Conference on Antimicrobial Agents and Chemotherapy, Toronto, Ontario, Canada. Abstract no 662.

[A.55] Uwamahoro M.J., S. Carryn, J.-M. Michot, M.-P. Mingeot-Leclercq, P.M. Tulkens & F. Van Bambeke. 2000. Modulation of the cellular accumulation of ciprofloxacin and moxifloxacin in J774 macrophages. In 40th Interscience Conference on Antimicrobial Agents and Chemotherapy, Toronto, Ontario, Canada. Abstract no 2268. [A.54] Tyteca, D., F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 2000. Azithromycin, a lysosomotropic antibiotic, impairs endocytosis in cultured macrophages. In 41st International Conference on the Biochemistry of Lipids, Haale (Saale), Germany. Abstract T3/28. [A.53] Quetin-Leclercq J., G. Schomer, F. Van Bambeke, M.-C. De Pauw-Gillet, M. Frederich, M. Tits, L. Angenot & M.-P. Mingeot-Leclercq. 1999. Lysosomal phospholipases inhibition by emetine, strychnopentamine and usambarensine. In 2000 Years of natural products research � past, present and future, Amsterdam, The Netherlands. [A.52] Tyteca, D., K. Leys, F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 1999. Macrolide antibiotics (azithromycin and erythromycylamine) perturb the early stages of endocytosis in phagocytic and non-phagocytic cells. In 39th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, Cal. Abstract no. bs 1756. [A.51] Tyteca, D., F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 1999. Azithromycin, a lysosomotropic antibiotic, perturbs the early stages of endocytosis in phagocytic and non-phagocytic cells. In European Congress of Cell Biology, Bologna, Italy. D-147. [A.50] El Mouedden, M., G. Laurent, M.P. Mingeot-Leclercq & P.M. Tulkens. 1998. Apoptose induite par la gentamicine, un antibiotique lysosomotrope, en relation avec la surcharge lysosomale. In 2eme Congrès Scientifique VML (Vaincre les Maladies Lysosomales), Dijon, France. [A.49] El Mouedden, M., G. Laurent, M.-P. Mingeot-Leclercq & P.M. Tulkens. 1998. Comparative studies of aminoglycoside-induced apoptosis in rat kidneys: study with gentamicin, netimicin, amikacin, and isepamicin. In 38th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego, Abstract no A-88 [A.48] Tyteca, D., F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 1998. Perturbation of membrane traffic induced in cultured cells by azithromycin, a lysosomotropic antibiotic. Arch. Int. Physiol. Biochim. Biophys. 106:B28 [A.47] Tyteca, D., F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 1998. Perturbation of endocytosis induced by azithromycin in a model of cultured cells. In Satellite symposium on pharmacodynamics of antimicrobials? International Society of Antiinfective Pharmacology, San Diego, Cal. [A.46] El Mouedden, M., G. Laurent, M.P. Mingeot-Leclercq & P.M. Tulkens. 1997. Understanding the mechanism of aminoglycoside-induced apoptosis and establishing its significance in relation with clinical toxicity of these drugs. In 9ème Réunion scientifique de la Société Belge d'Infectiologie et de Microbiologie Clinique. 11. [A.45] Kerkhofs, A., M.P. Mingeot-Leclercq & E. Sonveaux. 1997. Pénétration intracellulaire d'oligonucléotides et de peptides par encapsulation dans des liposomes. In Semaine scientifique des sciences de la vie, Bruxelles, Belgium. [A.44] Kerkhofs, A., F. Van Bambeke, E. Sonveaux, P.M. Tulkens & M.P. Mingeot-Leclercq. 1997. pH-sensitivity, leakage efficiency and endocytosis pathway of pH-sensitive liposomes DOPE/CHEMS AND DOPE/OA. In 7th Forum of pharmaceutical sciences, Blankenberg, Belgium.

[A.43] Kerkhofs, A., F. Van Bambeke, P.M. Tulkens, C. Remacle & M.P. Mingeot-Leclercq. 1997. Could pH-sensitive liposomes be used as drug delivery systems into cells. [A.42] Montenez, J.P., F. Van Bambeke, J. Piret, R. Brasseur, P.M. Tulkens & M.P. Mingeot-Leclercq. 1997. Comparative studies of lysosomal phospholipidosis induced by four macrolides. In 37th Interscience Conference on Antimicrobial Agents and Chemotherapy, Toronto, Canada. Abstract F266. [A.41] El Mouedden, M., G. Laurent, J. Piret, M.P. Mingeot-Leclercq & P.M. Tulkens. 1996. Correlation between lysosomal phospholipidosis and apoptosis induced by aminoglycosides: in vitre comparative study with gentamicin, netilmicin, and isepamicin. In 36th Interscience Conference on Antimicrobial Agents and Chemotherapy, New Orleans, La. Abstract no. A86. [A.40] Kerkhofs, A., F. Van Bambeke, E. Sonveaux, P.M. Tulkens & M.P. Mingeot-Leclercq. 1996. pH-sensitive liposomes: pH-sensitivity, leakage efficiency, endocytosis, and natural targeting to the acidic compartments. In Liposome Advances (Progress in drug and vaccine delivery), London, England. A 42. [A.39] Mingeot-Leclercq, M.P., J.P. Montenez, F. Van Bambeke, A. Schanck, R. Brasseur & P.M. Tulkens. 1996. Interaction of cationic lysosomotropic antibiotics with negatively-charged membranes. II. Molecular characterization by biophysical andcomputer-aided conformational studies. In 24th Meeting of the Federation of European Biochemical Societies, Barcelona, Spain. PS23-48. [A.38] Montenez, J.P., F. Van Bambeke, M.P. Mingeot-Leclercq, J. Piret, R. Brasseur & P.M. Tulkens. 1996. Azithromycin causes an accumulation of lipids in lysosomes of cultured cells: Biochemical mechanism and potential relationship with its long-time retention in cells. In 36th Interscience Conference on Antimicrobial Agents and Chemotherapy, New Orleans, La. Abstract no. A85. [A.37] Tyteca, D., L. Belaabidia, J.P. Montenez, F. Van Bambeke, M.P. Mingeot-Leclercq, J. Piret & P.M. Tulkens. 1996. In vitro comparative studies of the interactions of gentamicin and azithromycin with membrane lipids: contribution to the understanding of the molecular mechanism by which they cause a kysosomal phospholipidosis in cultured cells. Arch. Physiol. Biochem. 104:B22. [A36] Van Bambeke, F., J.P. Montenez, M.P. Mingeot-Leclerq, L. Belaabidia, D. Tyteca & P.M. Tulkens. 1996. Interaction of cationic lysosomotropic antibiotics with negatively-charged membranes. I. Binding, inhibition of lysosomal phospholipase A1 and influence on bilayer aggregation/fusion. In 24th Meeting of the Federation of European Biochemical Societies, Barcelona, Spain. PS23-47. [A.35] Van Bambeke, F., J.P. Montenez, M.P. Mingeot-Leclercq, J. Nachega, P.J. Courtoy & P.M. Tulkens. 1996. Perturbation of endosome-lysosome fusion induced in cultured cells by two lysosomotropic antibiotics (azithromycin, gentamicin) causing a lysosomal phospholipidosis. In Page-Wood International Symposium on Membrane Traffic in Health and Disease, Cleveland, Ohio. Abstract no. 42. [A.34] Schanck, A., F. Van Bambeke, P.M. Tulkens & M.P. Mingeot-Leclercq. 1995. 31P NMR of negatively-charged liposomes interacting with aminoglycosides. In Comptes-rendus du GERM XIV, Anglet-Biarritz. 60. [A33] Van Bambeke, F., M.P. Mingeot-Leclercq, A. Schanck, R. Brasseur & P.M. Tulkens. 1995. Aminoglycosides aggregate membranes and prevent phospholipid reorganization: comparative

studies with gentamicin (G), amikacin (A), and isepamicin (I) and correlation with nephrotoxicity. In 35th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, California. Abstract no. 913. [A.32] Van Bambeke, F., P.M. Tulkens, A. Schanck & M.P. Mingeot-Leclercq. 1995. Aminoglycoside antibiotics cause aggregation of negatively-charged membranes but inhibit the lipid reorganization necessary for their fusion. In 19th International Congress of Chemotherapy, Montreal, Quebec, Canada/Can. J. Infect. Dis. 6 suppl. C. Abstract no. 3009. [A31] Van Bambeke, F., P.M. Tulkens, A. Schanck & M.P. Mingeot-Leclercq. 1995. Deciphering the toxicological parameters of aminoglycoside nephrotoxicity: a combined biophysical and biochemical study of gentamicin, amikacin and isepamicin in interaction with phosphatidylinositol-containing lipid vesicles. In Satellite symposium on pharmacodynamics of antimicrobials, Quebec city, Quebec, Canada. Abstract no. 27. [A.30] Kishore, B.K., P. Maldague, G. Laurent, M.-P. Mingeot-Leclercq, S. Ibrahim & P.M. Tulkens. 1994. Acute renal failure induced by aminoglycoside antibiotics: pathophysiology, clinical implications and development of protective measures. In Asian Nephrology - Proceedings of Fifth Asian Pacific Congress of Nephrology (1992). Sakhuja V. and Malhotra H.S., edit., Oxford University Press, New Delhi. 401-409. [A.29] Mingeot-Leclercq, M.P., L. Depienne, F. Van Bambeke, J.P. Montenez & P.M. Tulkens. 1994. Interaction of aminoglycoside and macrolide antibiotics with membranes. effect on Ca2+ permeability.. Cell Biol. Int. Rep. 17/2:--. [A.28] Mingeot-Leclercq, M.P., S. Kotretsou, S. Constantinou-Kokotou, M.P. Georgiadis, R. Brasseur & P.M. Tulkens. 1994. Synthesis, microbiological and in vitro toxicological evaluation of amino acid and peptide derivatives in position 6 prime of kanamycin A and netilmicin. In 34th Interscience Conference on Antimicrobial Agents and Chemotherapy, Orlando, Fl. Abstract no. F140. [A.27] Mingeot-Leclercq, M.P., F. Van Bambeke, A. Schanck, L. Depienne, J.P. Montenez & P.M. Tulkens. 1994. Effect of aminoglycoside and macrolide antibiotics on membrane permeability. In FEBS Special Meeting Biological Membranes, Helsinki, Finland. ---. [A.26] Mingeot-Leclercq, M.P., F. Van Bambeke, A. Schanck, L. Depienne, J.P. Montenez & P.M. Tulkens. 1994. Effect of aminoglycoside and macrolide antibiotics on membrane permeability. In FEBS Special Meeting Biological Membranes, Helsinki, Finland. 248. [A.25] Van Bambeke, F., M.P. Mingeot-Leclercq, L. Depienne & P.M. Tulkens. 1994. Evaluation of the capacity of aminoglycoside antibiotics to induce aggregation or fusion of liposomes, measured by the dequenching of the fluorescence of octadecylrhodamine B chloride (R18). Cell Biol. Int. Rep. 17/2:--. [A.24] Van Bambeke, F., M.P. Mingeot-Leclercq, L. Depienne & P.M. Tulkens. 1994. Evaluation of aminoglycoside (AG) antibiotics capacity to induce aggregation or fusion of liposomes, by using 3 complementary fluorescence techniques. In FEBS Special Meeting Biological Membranes, Helsinki, Finland. 247. [A.23] Van Bambeke, F., M.P. Mingeot-Leclercq, L. Depienne & P.M. Tulkens. 1994. Aminoglycosides induce aggregation but not fusion of liposomes, as evidenced by biophysical and morphological approaches. In 34th Interscience Conference on Antimicrobial Agents and Chemotherapy, Orlando, Fl. Abstract no. A108a.

[A.22] Van Bambeke, F., M.P. Mingeot-Leclercq, A. Schanck, R. Brasseur & P. Tulkens. 1994. Aminoglycoside antibiotics induced alterations in membrane permeability of liposomes to increasing molecular weight markers. Cell Biol. Int. Rep. 17/2:--. [A.21] Mingeot-Leclercq, M.P., F. Van Bambeke, R. Brasseur & P.M. Tulkens. 1993. Aminoglycoside-induced increase in membrane permeability. II. Studies with cultured cells. In 11th International Biophysics Congress, Budapest, Hungary. In press. Abstract no. C5.32. [A.20] Van Bambeke, F., M.P. Mingeot-Leclercq & P.M. Tulkens. 1993. Alterations in membrane permeability induced by aminoglycoside antibiotics. Studies on liposomes and cells. Arch. Int. Physiol. Biochim. Biophys. 101:B21. [A.18] Van Bambeke, F., M.P. Mingeot-Leclercq, L. Depienne & P.M. Tulkens. 1993. Aminoglycoside (AG) induced increase of membrane permeability in liposomes and cultured cells. In 33rd Interscience Conference on Antimicrobial Agents and Chemotherapy, New Orleans, Louisiana. Abstract no. 218B. [A.17] Van Bambeke, F., M.P. Mingeot-Leclercq, L. Depienne, A. Schanck & P.M. Tulkens. 1993. Aminoglycoside-induced increase in membrane permeability. I. Studies with large unilamellar vesicles. In 11th International Biophysics Congress, Budapest, Hungary. Abstract no. C5.31. [A.16] Busson, R., J. Delcourt, P. Claes, H. Vanderhaeghe, P.M. Tulkens & M.P. Mingeot-Leclercq. 1990. Chemical modification of kanamycin B in the 6"-position. In Intern. Symposium Carbohydrate Chemistry, Yokoama, Japan. Abstract no. D032. [A.15] Claes, P., A. Van Schepdael, R. Busson, H. Vanderhaeghe, P.M. Tulkens & M.P. Mingeot-Leclercq. 1990. Synthesis of 6"-modified 1-C-CH 2 OH-kanamycin B analogues. In Intern. Sympium on Carbohydrate Chemistry, Yokoama, Japan. D031. [A.14] Piret, J., M.P. Mingeot-Leclercq, S. Delfosse & P.M. Tulkens. 1990. Relationship between binding of gentamicin to negatively-charged lipids and inhibition of lysosomal phospholipase A1 towards phosphatidylcholine. J. Pharm. Belg. 45:82. [A13] Van Schepdael, A., H.J. Vanderhaeghe, R. Busson, P. Claes, L. Verbist, M.P. Mingeot-Leclercq & P.M. Tulkens. 1990. Synthesis, microbiological and in vitro toxicological evaluation of kanamycin B analogues modified in 6" and in 6" and C1 positions. In 30th Interscience Conference Antimicrobial Agents and Chemotherapy, Atlanta, Georgia. Abstract no. 435. [A12] Delfosse, S., M.P. Mingeot-Leclercq, J. Piret & P.M. Tulkens. 1989. Influence of gangliosides on the activity of lysosomal phospholipases and their inhibition by aminoglycoside antibiotics. Arch. Intern. Physiol. Biochim. 97:B138. [A11] Mingeot-Leclercq, M.P. & P.M. Tulkens. 1989. Influence of different negatively-charged phospholipids on the activity of lysosomal phospholipases towards phosphatidylcholine. In 19th Meeting of the Federation of the European Biochemical Societies, Rome, Italy. Abstract no. MO125. [A.10] Mingeot-Leclercq, M.P., R. Brasseur, R. Wagner, J. Piret & P.M. Tulkens. 1989. Inhibition of phospholipase A1 activity towards phosphatidylcholine induced by gentamicin: effect of different negatively-charged phospholipids.. In 19th Meeting of the Federation of European Biochemical Societies, Rome, Italy. Abstract no. FR167. [A.9] Kishore, B.K., M.P. Mingeot-Leclercq, Z. Ka,llay, D. Beauchamp & P.M. Tulkens. 1988. Mechanism of the protection afforded by poly-L-aspartic acid against gentamicin-induced

nephrotoxicity: poly-L-aspartic acid binds gentamicin in lysosomes. In 28th Interscience Conference Antimicrobial Agents and Chemotherapy, Los Angeles, CA. Abstract no. 293. [A.8] Mingeot-Leclercq, M.P., R. Brasseur, J.M. Ruysschaert, A. Schanck, M. Deleers & P.M. Tulkens. 1987. Conformational and biophysical analysis of the interactions between gentamicin or bis(betadiethylaminoethylether)hexestrol and negatively-charged phospholipid layers. In Structure and Function of Molecules Affecting the Properties of Cellular Membranes, Paris, France. Abstract no. P.19. [A.7] Mingeot-Leclercq, M.P., G. Laurent & P.M. Tulkens. 1987. Mechanism of inhibition of lysosomal phospholipases by gentamicin, an aminoglycoside antibiotic. Arch. Int. Physiol. Biochim. 95:B85. [A.8] Mingeot-Leclercq, M.P., M.F. Ronvaux-Dupal, M. Deleers, A. Schanck & P.M. Tulkens. 1987. Changes in the morphology of negatively-charged liposomes induced by gentamicin and bis(beta-diethylaminoethylether)hexestrol. In Structure and function of Molecules Affecting the Properties of Cellular Membranes, Paris, France. Abstract no. P.18. [A.7] Schanck, A., M.P. Mingeot-Leclercq & P.M. Tulkens. 1987. 31P NMR and fluorescence depolarization study of model membranes in the presence of drugs inducing phospholipidosis. In 9th International Biophysics Congress, Jerusalem, Israel. 175. [A.6] Tulkens, P.M. & M.P. Mingeot-Leclercq. 1987. Drug-induced lysosomal phospholipidosis: biophysical and biochemical studies with aminoglycoside antibiotics and cationic amphiphiles. In Lipid Storage disorders: biological and medical aspects (NATO Avanced Research Workshop) Toulouse. 50. [A.5] Mingeot-Leclercq, M.P., G. Laurent, A. Schanck, M.F. Ronveaux-Dupal, M. Deleers & P.M. Tulkens. 1986. Molecular mechanisms of aminoglycoside-induced lysosomal phospholipidosis. In IXth International Symposium on Drugs Affecting Lipid Metabolism, Florence, Italy. 195. [A.4] Tulkens, P.M., G. Laurent, M.B. Carlier, P. Lambricht, M.P. Mingeot-Leclercq & J.M. Ruysschaert. 1986. In vitro model and semi-empirical conformational analysis to study and compare the intrinsic nephrotoxicity of aminoglycoside antibiotics. In Recent advances in Chemotherapy. J. Ishigami, edit., University of Tokyo Press, Tokyo. 2634-2635. [A3] Carlier, M.B., R. Brasseur, G. Laurent, M.P. Mingeot-Leclercq, J.M. Ruysschaert & P.M. Tulkens. 1985. Use of an in vitro model to assess the aminoglycoside-phospholipid interactions, in relation to their nephrotoxicity. In Intern. Conference on Practical in vitro Toxicology, Reading, Berks., U.K. Abstract no. 6.21. [A2] Mingeot-Leclercq, M.P., G. Laurent & P.M. Tulkens. 1985. The mechanism of gentamicin-induced inhibition of phosphatidylcholine hydrolysis. In 13th Congress of the International Union of Biochemistry, Amsterdam,The Nederlands. Abstract no. TH/443. [A1] Tulkens, P.M., G. Laurent, M.B. Carlier, M.P. Mingeot-Leclercq & J.M. Ruysschaert. 1985. In vitro model and semi-empirical conformational analysis to study and to compare the intrinsic nephrotoxicity of aminoglycoside antibiotics. In 14th Intern. Congress of Chemotherapy, Kyoto. Abstract no. S24-4.