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1 Abhishek Kumar Mishra (PhD) (1/6/1982) Corresponding address: 4040, Department of Pharmacology, AIIMS, New Delhi-29, India Email and LinkedIn: [email protected] Contact No. and Skype address: +91-8447197715, 9958298072 [email protected] Objective: To promote the goodwill of the institute for better output through a disciplined, organized and progressive ways with my sincere hard work and utmost endeavours in the task entrusted to me and keep growing at every step of my life. Academia Year Degree/Result University/ Institution Marks (%) February 2015 PhD All India institute of medical sciences, New Delhi Awarded July 2006 M.Sc. (Biotechnology) Punjabi university, Patiala (Punjab) 68.45 July 2000 BSc. (Life Science) St. Andrews P G college, Gorakhpur 59.94 PhD thesis and Research projects PhD Thesis Title: Development of small interfering RNAs (siRNAs) against Coxsackievirus A24 causing eye infections Senior Research Fellow in an AYUSH, Ministry of Health and family welfare funded project “Preliminary pharmacological and safety studies of Homeopathic drugs” in the department of Pharmacology, AIIMS, New Delhi (9/2/2015 to 12/06/2015). Senior Research Fellow in an Indian Council of Medical Research funded and awarded research project Microsporidial keratoconjunctivitis in Delhi: A prospective study using rapid molecular diagnostics and cytologyin the Department of Ocular Microbiology, All India Institute of Medical Sciences, New Delhi, (February, 2013 to February, 2015) Senior Research Fellow in Department of Biotechnology funded research project Development of small interfering RNA (siRNA) against Enterovirus70 and CoxsackievirusA24 causing acute haemorrhagic conjunctivitisin the Department of

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Abhishek Kumar Mishra (PhD)

(1/6/1982)

Corresponding address: 4040, Department of Pharmacology, AIIMS, New Delhi-29, India

Email and LinkedIn: [email protected]

Contact No. and Skype address: +91-8447197715, 9958298072 [email protected]

Objective: To promote the goodwill of the institute for better output through a disciplined,

organized and progressive ways with my sincere hard work and utmost endeavours in the task

entrusted to me and keep growing at every step of my life.

Academia

Year Degree/Result University/ Institution Marks (%)

February

2015 PhD

All India institute of medical

sciences, New Delhi Awarded

July 2006 M.Sc.

(Biotechnology) Punjabi university, Patiala (Punjab) 68.45

July 2000 BSc. (Life Science)

St. Andrews P G college, Gorakhpur 59.94

PhD thesis and Research projects

PhD Thesis Title: Development of small interfering RNAs (siRNAs) against

Coxsackievirus A24 causing eye infections

Senior Research Fellow in an AYUSH, Ministry of Health and family welfare funded

project “Preliminary pharmacological and safety studies of Homeopathic drugs” in the

department of Pharmacology, AIIMS, New Delhi (9/2/2015 to 12/06/2015).

Senior Research Fellow in an Indian Council of Medical Research funded and awarded

research project “Microsporidial keratoconjunctivitis in Delhi: A prospective study

using rapid molecular diagnostics and cytology” in the Department of Ocular

Microbiology, All India Institute of Medical Sciences, New Delhi, (February, 2013 to

February, 2015)

Senior Research Fellow in Department of Biotechnology funded research project

“Development of small interfering RNA (siRNA) against Enterovirus70 and

CoxsackievirusA24 causing acute haemorrhagic conjunctivitis” in the Department of

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Ocular Microbiology, All India Institute of Medical Sciences, New Delhi (March, 2010 to

July ,2012)

Junior Research Fellow in an Indian Council of Medical Research funded research project

“Evaluation of polymerase chain reaction assay for Herpes Simplex Virus-I (HSV)

detection in tear specimen of suspected viral keratitis, particularly stromal and

atypical epithelial keratitis.” in the Department of Ocular Microbiology, All India

Institute of Medical Sciences, New Delhi (July,2007 to March,2010)

CSIR-UGC NET(Life Science) Qualified (310296/23/12/2007)

Trainee in Biotech Consortium India Limited sponsored training programme in the area of

“Human Papillomavirus Detection assay” (29.11.06 -28.05.07)

Masters project “Cytotoxicity assay of lung cancer cell line -A549 by using m-PEG-

DAAO conjugates” at Punjabi University, Patiala, India (01.12.05 - 01.05.06)

Trainee in CSIR sponsored CIMAP summer school on “Molecular techniques in Bio

prospection and biodiversity analysis” at Central Institute of Medicinal and Aromatic

Plants, Lucknow, India (15.06.05 -30.07.05)

Scientific and technical skills

Working micro-organisms: Mycobacterium tuberculosis, Acanthamoeba, Coxsackie

virus A24, Human Enterovirus 70, Human Adenovirus, Microsporidia, Herpes simplex

virus-I, Human Cytomegalovirus and Chlamydia trachomatis.

Molecular Biology: PCR, Molecular cloning, Western, Northern and Southern blotting,

in vitro transcription, Interferon assay, RNA interference experiments and other

techniques involved in molecular biology experiments, Flow cytometry

Animal cell culture: Media and reagents preparation, Maintenance of cell lines,

Propagation of virus in cell lines, Transfection and cytotoxicity assays.

Microscopy: Bright field, Phase contrast and Immunofluorescence microscopy

Bioinformatics and Biostatistical tools: shRNA and siRNA designing, Primer express,

Bio Edit, Gene runner, Rasmol, ClustalW, MEGA 6.0, IBM SPSS, Graph pad PRISM

Scientific papers

Satpathy G, Mishra AK, Tandon R, Sharma MK, Sharma A, Nayak N, Titiyal JS,

Sharma N. Evaluation of tear samples for Herpes Simplex Virus 1 (HSV) detection in

suspected cases of viral keratitis using PCR assay and conventional laboratory diagnostic

Br J Ophthalmology. 2011 Mar; 95(3):415-8

Development of effective small interfering RNAs (siRNAs) to treat acute hemorrhagic

conjunctivitis in Coxsackie virus A24 infections (Paper communicated)

Outbreak of Adenovirus in a tertiary care hospital of North India-Emergence of a new

ocular strain? (Paper communicated)

Scientific abstracts and presentations

Satpathy G, Patnayak D, Mishra AK and Panda SK. siRNA mediated inhibition of

Entero Virus 70 (EV 70) causing acute haemorrhagic conjunctivitis in tissue culture

system. Presented in 110th General meeting of American Society of Microbiology, May

23rd- 27th, 2010, San Deigo, CA, USA. pp F 2147 (ABSTRACT)

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Satpathy G, Patnayak D, Mishra AK, Panda SK. In vitro inhibition of conjunctivitis

causing Enteroviruses (EV 70 & Coxsackievirus A 24) by RNAi using shRNAs.

Presented in 9th International Symposium on Positive – strand RNA viruses. May 17th –

23rd, 2010, Atlanta, GA, USA. pp 61. (ABSTRACT)

Mishra AK, Satpathy G. Inhibition of Coxsackievirus A24 by sequence- specific small

Interfering RNAs (siRNAs). Presented in ASIA ARVO 2013, October 28-31. New Delhi,

India, pp 92. (POSTER)

Mishra AK, Satpathy G. Development of effective small interfering RNAs (siRNAs) to

treat acute hemorrhagic conjunctivitis by Coxsackie virus A24 infections Presented in

SYSCON on Recent Advances in biological sciences held on 10th December 2014 at All

India Institute of Medical Sciences, New Delhi, India (POSTER)

Other Scientific involvements

Organizing committee member of SIHAM ( March 2010) at All India Institute of

Medical Sciences , New Delhi , India

Organizing committee member of CME (April,2009) at All India Institute of Medical

Sciences , New Delhi , India

Participated in mini international symposium on “Drug discovery and development :

case studies”organised by Department of Pharmacology, All India Institute of Medical

Sciences held on 30th October 2007at AIIMS,New Delhi,India.

Participated in National Symposium on Immunity to Infection organised by SYS-

AIIMS, held on 8th October 2007at All India Institute of Medical Sciences,New

Delhi,India.

Participaed in National Symposium on Nanotechnology in medicine organized and

conducted by SYS- All India Institute of Medical Science ,held on 13 Jan 2007, New

Delhi,India.

Successfully completed a Flow Cytometry BD FACSOrient (BD FACS Calibur) training

programme from 27th-29th May, 2015 at BD-JH FACS Academy, New Delhi.

References

Dr Gita Satpathy

Professor and Head,

Department of Microbiology

All India Institute of Medical Sciences

Ansari Nagar, New Delhi-110029

Email:[email protected]

Phone: 011-26593171

Dr S K Panda

Professor and Head

Department of Pathology

All India institute of Medical Sciences

Ansari Nagar, New Delhi-110029

Email:[email protected]

Phone:011-26594924

Dr Radhika Tandon

Professor of Ophthalmology

Dr R P Centre for Ophthalmic Sciences

All India Institute of Medical Sciences

Ansari Nagar, New Delhi-110029

Email: [email protected]

Phone: 011-26593194

Dr Neelam Verma

Professor of Biotechnology

Department of Biotechnology

Punjabi University, Patiala,

Punjab-147002

Email:[email protected]

Phone: +98-9815710298