Lake Mondsee

Kammerlander Barbara1, Nachbaur Laura1, Posch Thomas2, Sonntag Bettina1, Stoeck Thorsten3 and D-A-CH Consortium*1Research Department for Limnology, Mondsee, University of Innsbruck, Austria. 2Limnological Station, University of Zürich, Switzerland. 3Department of Ecology, University of Kaiserslautern, Germany.

*D-A-CH Consortium: Deng Li4, Höllerer Hannes1, Jersabek Christian5, Ring Natalja1, Scheffel Ulrike1, Schmidt Johanna1, Schmidt-Halewicz Sabine6, Teubner Katrin7.. 4Institute of Virology, Helmholtz Zentrum München, Germany 5Division of Animal Structure & Function, University of Salzburg, Austria. 6 LimSa [Gewässerbüro], Konstanz, Germany. 7Department of Limnology & Biological Oceanography, Faculty of Life Sciences, University of Vienna, Austria

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Acknowledgements: We thank M. Aigner, V. Bergkemper, A. Cieplinski, E. Doubrava, L. Frühe, F. Leitner, X. Lu, K. Mayrhofer, and M. Zeilinger for help during sampling.

Selected references: Dokulil, 2005; Ficker et al., 2017; Griebler & Posch, 2001; Šimek et al., 2014; Sommer et al., 2012; Steele et al., 2011; Zingel et al., 2012; Xia etal., 2011.Photos: Mondsee: B. Kammerlander, Ciliates: B. Kammerlander, B. Sonntag; Zurichsee: T. Posch; Graph ‘Blackbox’ modified after Griebler & Posch, 2001, Streble & Krauter,2002.

Basis for experimental hypotheses

Specific networks of dominant ciliatesNetwork analyses

in 1 mL water of Lake Mondsee

Example: July 2016

D-A-CH project

Phytoplankton

Abiotic dataPredatory

zooplankton

Salzkammergut area inAustria

481 m a.s.l.

Max. depth 68.3 m

Surface area 14.2 km2

oligo-mesotrophic

holomictic-dimictic

HNF

Oligohymenophorea

Litostomatea

Prostomatea

Spirotrichea

Zooplankton (Crustacea+Rotifera+Dreissena larvae)

Chlorophyll a

Bacteria

(mean abundance data)

Abiotic parameterse.g., nutrients, oxygen,

temperature

SamplingBiweekly

June 2016 to

May 2017

Vertical depth profiles

Lake Mondsee Lake Zurich

Lake biotaCiliates, bacteria, HNF,

phyto-, zooplankton, viruses+

environmental parameters

+nutrients

Identification+

Cultivation

+Sequencing

(single cell PCR)

Next Generation Sequencing (Illumina)

�V9 region of the 18S SSU

Morphology &Limnology

Isolation & Cultivation

Molecular Sequencing

Network analyses & Experiments

Co-occurrence networks in aquatic food webs: ciliates as models

Key question: which factors explain the variability in ciliateassemblages in time (seasonality) and space (spatial distribution)?

Abiotic factors

Phytoplankton

Predatory zooplankton

Bacteria & HNF

Predatory ciliates

Bacteria106 Ind. mL-1

Ciliates1-35 Ind. mL-1

Heterotrophic nanoflagellates (HNF)

102-103 Ind. mL-1

Rotifers0-1 Ind. mL-1

Crustaceans0-1 Ind. mL-1Algae

104-106 Ind. mL-1

coccoid Cyanobacteria103 -105 Ind. mL-1

Aquatic food web

Bacteria

small

large

e

mid.

Bac a

small

Ciliates

mixotrophic

Algae 1 Algae 2 Algae

abiotic + biotic

parameters

Rotifera

CrustaceaDreissena larvae

Ciliates

omnivore

Ciliates

unknown

Ciliates

bacterivore

Ciliates

algivore

Ciliates

predatory

Project: I2238-B25

Viruses107 Ind. mL-1

Inventory of the ‘blackbox’0 20 40 60 80 100

Balanion planctonicumHistiobalantium bodamicum

Urotricha spp. <20 µmTintinnidium/Tintinnopsis

Halteria/Pelagohalteria heterotrophicRimostrombidium brachykinetum

Askenasia acrostomiaPelagohalteria cirrifera

Rimostrombidium humileColeps spp. mixotrophic

Rimostrombidium spp.Urotricha castalia

Urotricha spp. >20 µmRhabdoaskenasia minimaLimnostrombidium spp.

Rimostrombidium '15/16'Urotricha 'grüni'

Pelagostrombidium spp.Lagynophrya spp.

Urotricha macrostomaAskenasia chlorelligeraPelagovorticella natans

Scutico spp.Rimostrombidium lacustris

Cinetochilum margaritaceumHalteria/Pelagohalteria mixotrophic

Pelagohalteria viridisVorticella aquadulcis complex

Didinium/Monodinium chlorelligerumAskenasia spp. heterotrophic

Rimostrombidium hyalinumVorticella vernalis

Epistylis anastaticaMembranicola tamari

Askenasia volvoxCyclidium cf. heptatrichum

Urotricha furcataEpistylis procumbens

Peritrich swarmersAskenasia 'mini'Mesodinium spp.

Urotricha pseudofurcataCodonella cratera

Cothurnia sp.Hypotrichia sp.

Urotricha globosaVorticella chlorellataUrotricha platystoma

Monodinium spp. Paradileptus elephantinus

Urotricha venatrixUrotricha farctaCyrtolophosis sp.

A B

C D

E F