DAFTAR ISI

DAFTAR ISI.............................................................................................................i

DAFTAR TABEL..................................................................................................iii

DAFTAR GAMBAR..............................................................................................iv

DAFTAR GRAFIK.................................................................................................iv

BAB I PENDAHULUAN

1.1 Latar belakang.....................................................................................1

1.2 Rumusan Masalah...............................................................................3

1.3 Tujuan Penelitian................................................................................3

BAB II TINJAUAN PUSTAKA

2.1 Jerami Padi..........................................................................................4

2.2 Lignoselulosa......................................................................................5

2.3 Selulosa...............................................................................................6

2.4 Hemiselulosa.......................................................................................7

2.5 Lignin..................................................................................................8

2.6 Pretreatment Lignoselulosa................................................................9

2.7 Delignifikasi pada Bejana Bertekanan 1 bar.....................................11

2.8 Bacillus subtilis.................................................................................12

2.9 Bacillus circulans...................................................................................13

2.10 Enzim selulase.................................................................................14

2.11 Glukosa...........................................................................................15

2.12 Avicel..............................................................................................16

2.13 Selobiosa.........................................................................................17

BAB III METODE PENELITIAN

3.1 Metode Penelitian..............................................................................18

3.2 Alat dan bahan..................................................................................18

3.2.1 Alat.................................................................................................18

3.2.2 Bahan..............................................................................................18

3.3 Skema Kerja Penelitian.....................................................................19

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BAB IV HASIL DAN PEMBAHASAN

4.1 Pretreatment Media..........................................................................20

4.1.1 Delignifikasi pada Bejana Bertekanan 1 bar..................................21

4.2 Uji Lignin dengan Metode Chesson dan Van Soest.........................21

4.2.1 Metode Van Soest...........................................................................23

4.3 Analisa Aktivitas Enzim...................................................................25

BAB V PENUTUP

5.1 Simpulan...........................................................................................34

5.2 Saran..................................................................................................34

DAFTAR PUSTAKA............................................................................................35

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DAFTAR TABEL

Tabel 2.1 Komponen jerami padi.....................................................................5

Tabel 2.2 Komposisi lignoselulosa dalam beberapa sumber............................6

Tabel 2.3 Metode perlakuan awal...................................................................10

Tabel 4.1 Uji kandungan hot water soluble, selulosa, hemiselulosa dan

lignin pada masing- masing ukuran jerami padi sebelum proses

pretreatment....................................................................................22

Tabel 4.2 Uji kandungan hot water soluble, selulosa, hemiselulosa dan

lignin pada masing- masing ukuran jerami padi setelah proses

pretreatment....................................................................................22

Tabel 4.3 Hasil analisa ADF jerami padi metode Van Soest..........................24

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DAFTAR GAMBAR

Gambar 2.1 Jerami padi.....................................................................................4

Gambar 2.2 Struktur biomassa lignoselulosa.....................................................5

Gambar 2.3 Konfigurasi dinding sel tanaman...................................................6

Gambar 2.4 Komponen pembentuk lignin.........................................................8

Gambar 2.5 Efek pretreatment.........................................................................11

Gambar 2.6 Bacillus subtilis.............................................................................13

Gambar 2.7 Karakteristik Bacillus circulans...................................................13

Gambar 2.8 Struktur glukosa...........................................................................15

Gambar 2.9 Struktur avicel..............................................................................16

Gambar 2.10 Struktur selobiosa.........................................................................17

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DAFTAR GRAFIK

Grafik 4.1 Aktifitas Selulase B. circulans dan B. subtilis substrat Avicel.......28

Grafik 4.2 Aktifitas Selulase B. circulans dan B. subtilis substrat Selobiosa..29

Grafik 4.3 Aktifitas Enzim Selulase B. Circulans dan B. subtilis dengan

substrat Avicel dengan aerasi (High, Low, dan Non Aerasi).........31

Grafik 4.4 Aktifitas Enzim Selulase B. Circulans dan B. subtilis dengan

substrat Selobiosa dengan aerasi (High, Low, dan Non Aerasi)....32

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