DAFTAR PUSTAKA - Diponegoro University | Institutional ...eprints.undip.ac.id/44210/9/Yurizal_G2A009137_BabVIIIKTI.pdf · DAFTAR PUSTAKA 1. Oktaviani WD, ... dan Orang Tua dengan

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Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in

Adults (Adult Treatment Panel III). Executive

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11. Asmadi. Teknik Prosedural Keperawatan: Konsep dan Aplikasi Kebutuhan

Dasar Klien. Jakarta: Penerbit Salemba Medika; 2008.

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13. Pilar S, Tamara S, Pablo V, Maria E. Antioxidant Properties of Brassica

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[internet]. 1995 [cited 2013 Jan 17]; 62(6):1462S-1476S. Available from:

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19. Sya’bani M. Buku Ajar Ilmu Penyakit Dalam Jilid II Edisi ketiga. Balai

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LAMPIRAN

Lampiran 1. Ethical Clearane

Lampiran 2. Hasil analisis mikroskopik organ ginjalLampiran 2. Hasil analisis mikroskopik organ ginjal

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51

Lampiran 3. Metode baku histologik pemeriksaan jaringan

A. Cara pengambilan dan fiksasi jaringan

1) Mengambil jaringan hepar sesegera mungkin setelah tikus

didekapitasi (kurang dari 2 jam) dengan ukuran 1x1x1 cm3.

2) Memasukkan jaringan ke dalam larutan fiksasi dengan urutan

sebagai berikut:

a. Fiksasi dalam larutan buffer formalin 10%.

b. Dehidrasi dengan larutan alkohol 30% selama 20 menit I,

20 menit II, dan 20 menit III.

Lalu dilanjutkan alkohol 40% 1 jam

alkohol 50% 1 jam

alkohol 60% 1 jam

alkohol 70% 1 jam

alkohol 80% 1 jam

alkohol 90% 1 jam

alkohol 96% 1 jam

c. Larutan xylol alkohol 1:1 dengan waktu ± 24 jam.

d. Clearing dengan larutan xylol 1, 2, 3 dengan waktu

masing-masing 20 menit, sehingga jaringan terlihat tembus

pandang.

e. Xylol parafin 1:1 selama 20 menit/24jam dengan

dipanaskan dalam oven 60oC.

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f. Embeding dan blocking: parafin 1, 2, 3 selama 20 menit,

lalu jaringan dicetak blok parafin, kemudian didinginkan,

sehingga cetakan dapat dibuka.

g. Trimming: memotong balok-balok parafin sehingga

jaringan mudah dipotong.

B. Cara pemotongan blok

1) Menyiapkan kaca objek bersih.

2) Kaca objek diberi albumin di tengahnya.

3) Blok yang sudah disiapkan dipotong dengan ketebalan 5 mikron,

lalu dimasukkan air panas ±60oC. Setelah jaringan mengembang,

jaringan diambil menggunakan kaca objek yang sudah diberi

albumin.

4) Keringkan.

5) Paraffin yang ada pada kaca objek atau jaringan dihilangkan

dengan dipanaskan dalam oven ± 60oC atau dengan tungku.

C. Pewarnaan

Slide jaringan dimasukkan dalam:

1) Xylol 1, xylol 2, dan xylol 3 masing-masing 10 menit.

2) Rehidrasi dengan alkohol xylol selama 5 menit.

3) Bilas dengan alkohol 30%-96%, masing-masing ±30 menit.

4) Bilas aquadest 1x ± 10 menit.

5) Rendam dalam hematoksilin ± 10 menit.

6) Bilas dengan air mengalir sampai bersih.

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7) Bilas dengan aquadest lalu alkohol asam (alkohol+NaCl 0,9%)

8) Bilas alkohol 50%-96%.

9) Eosin ±2-5 menit.

10) Bilas alkohol 96% dua kali.

11) Bilas alkohol xylol.

12) Keringkan dengan kertas saring, langsung dibersihkan kotoran-

kotoran yang ada di sekitar jaringan.

13) Xylol 1 (5 menit), xylol 2 (5 menit), tetesi basam Canada, langsung

ditutup kaca penutup.

14) Maka jadilah preparat.

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Lampiran 4. Hasil analisis

1. Uji normalitas data Shapiro-wilk gambaran mikrokopis ginjal

Tests of Normality

Kelompok Kolmogorov-Smirnov(a) Shapiro-Wilk

Statistic df Sig. Statistic df Sig.Skor Rerata Kelompok Kontrol

(tikus+pakan standar dan minum ad libitum+diet kuning telur)

,334 5 ,071 ,884 5 ,329

Kelompok Perlakuan 1 (tikus+pakan standar dan minum ad libitum+diet kuning telur+jus kubis 2,5 ml/hari)

,147 5 ,200(*) ,995 5 ,994


,159 5 ,200(*) ,990 5 ,980

Kelompok Perlakuan 3 (tikus+pakan standar dan minum ad libitum+diet kuning telur+jus kubis 5 ml/hari)

,222 5 ,200(*) ,923 5 ,553

* This is a lower bound of the true significance.a Lilliefors Significance Correction

55

Descriptives

Kelompok Statistic Std. ErrorSkor Rerata Kelompok Kontrol (tikus+pakan

standar dan minum ad libitum+diet kuning telur)

Mean5,9200 ,42237

95% Confidence Interval for Mean

Lower Bound4,7473

Upper Bound7,0927

5% Trimmed Mean 5,9444Median 6,0000Variance ,892Std. Deviation ,94446Minimum 4,40Maximum 7,00Range 2,60Interquartile Range 1,40Skewness -1,102 ,913Kurtosis 2,533 2,000


Mean

3,8800 ,30067


Lower Bound3,0452

Upper Bound4,7148

5% Trimmed Mean 3,8778Median 3,8000

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Variance ,452Std. Deviation ,67231Minimum 3,00Maximum 4,80Range 1,80Interquartile Range 1,20Skewness ,147 ,913Kurtosis ,154 2,000


Mean

3,5200 ,24166


Lower Bound2,8490

Upper Bound4,1910

5% Trimmed Mean 3,5222Median 3,6000Variance ,292Std. Deviation ,54037Minimum 2,80Maximum 4,20Range 1,40Interquartile Range 1,00Skewness -,183 ,913Kurtosis -,681 2,000


Mean

4,6800 ,68877

57


Lower Bound2,7677

Upper Bound6,5923

5% Trimmed Mean 4,7000Median 4,2000Variance 2,372Std. Deviation 1,54013Minimum 2,60Maximum 6,40Range 3,80Interquartile Range 2,80Skewness -,209 ,913Kurtosis -1,199 2,000

2. Uji homogenitas varian Levene test gambaran mikrokopis ginjal

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.Skor Rerata Based on Mean 2,249 3 16 ,122

Based on Median 1,140 3 16 ,363Based on Median and with adjusted df

1,140 3 9,407 ,382

Based on trimmed mean 2,266 3 16 ,120

58

3. Uji beda Oneway ANOVA gambaran mikrokopis ginjal

ANOVA

Skor Rerata

Sum of Squares df Mean Square F Sig.Between Groups 16,968 3 5,656 5,645 ,008Within Groups 16,032 16 1,002Total 33,000 19

4. Uji Post Hoc-LSD gambaran mikrokopis ginjal

Multiple Comparisons

Dependent Variable: Skor Rerata LSD

(I) Kelompok (J) Kelompok

Mean Difference (I-

J) Std. Error Sig. 95% Confidence Interval

Lower BoundUpper Bound Lower Bound Upper Bound Lower Bound

Kelompok Kontrol (tikus+pakan standar dan minum ad libitum+diet kuning telur)


2,04000(*) ,63309 ,005 ,6979 3,3821

59


2,40000(*) ,63309 ,002 1,0579 3,7421


1,24000 ,63309 ,068 -,1021 2,5821



-2,04000(*) ,63309 ,005 -3,3821 -,6979


,36000 ,63309 ,577 -,9821 1,7021


-,80000 ,63309 ,224 -2,1421 ,5421



-2,40000(*) ,63309 ,002 -3,7421 -1,0579

60


-,36000 ,63309 ,577 -1,7021 ,9821


-1,16000 ,63309 ,086 -2,5021 ,1821



-1,24000 ,63309 ,068 -2,5821 ,1021


,80000 ,63309 ,224 -,5421 2,1421


1,16000 ,63309 ,086 -,1821 2,5021

* The mean difference is significant at the .05 level.

61

5. Uji normalitas data pada semua kelompok kolesterol total dan trigliserida sebelum dan sesudah perlakuan

Tests of Normality

,318 5 ,109 ,776 5 ,051

,376 5 ,020 ,815 5 ,107

,212 5 ,200* ,905 5 ,439

,327 5 ,086 ,779 5 ,054

,291 5 ,193 ,841 5 ,169

,164 5 ,200* ,991 5 ,982

,275 5 ,200* ,825 5 ,128

,275 5 ,200* ,921 5 ,533

,226 5 ,200* ,858 5 ,221

,294 5 ,181 ,881 5 ,315

,218 5 ,200* ,963 5 ,832

,344 5 ,054 ,753 5 ,032

,275 5 ,200* ,869 5 ,261

,165 5 ,200* ,972 5 ,886

,296 5 ,175 ,764 5 ,040

,220 5 ,200* ,874 5 ,285

KelompokKontrol

Perlakuan1

Perlakuan2

Perlakuan3

Kontrol

Perlakuan1

Perlakuan2

Perlakuan3

Kontrol

Perlakuan1

Perlakuan2

Perlakuan3

Kontrol

Perlakuan1

Perlakuan2

Perlakuan3

PreKolesterolTotal

PreTrigliserida

PostKolesterolTotal

PostTrigliserida

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

62

6. Uji homogenitas data kolesterol total dan trigliserida sebelum dan sesudah perlakuan

Test of Homogeneity of Variance

12,149 3 16 ,000

1,189 3 16 ,345

1,189 3 8,102 ,373

10,712 3 16 ,000

2,736 3 16 ,078

1,518 3 16 ,248

1,518 3 9,908 ,270

2,666 3 16 ,083

3,190 3 16 ,052

,923 3 16 ,452

,923 3 11,315 ,461

3,052 3 16 ,059

1,525 3 16 ,246

,783 3 16 ,521

,783 3 10,040 ,530

1,421 3 16 ,273

Based on Mean

Based on Median

Based on Median andwith adjusted df

Based on trimmed mean

Based on Mean

Based on Median



Based on Mean

Based on Median



Based on Mean

Based on Median



PreKolesterolTotal

PreTrigliserida

PostKolesterolTotal

PostTrigliserida

LeveneStatistic df1 df2 Sig.

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7. Uji normalitas data post kolesterol total setelah transformasi data

Tests of Normality

,232 5 ,200* ,859 5 ,224

,286 5 ,200* ,890 5 ,355

,248 5 ,200* ,905 5 ,440

,336 5 ,067 ,757 5 ,034

KelompokKontrol

Perlakuan1

Perlakuan2

Perlakuan3

postkoltransStatistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

64

8. Paired t test kelompok kontrol prepost kolesterol total dan trigliserida

Paired Samples Statistics

36,9100 5 8,19799 3,66625

55,7980 5 16,82419 7,52401

98,6800 5 20,70633 9,26015

77,7200 5 15,45322 6,91089

PreKolesterolTotal

PostKolesterolTotal

Pair1

PreTrigliserida

PostTrigliserida

Pair2

Mean N Std. DeviationStd. Error

Mean

Paired Samples Correlations

5 -,090 ,885

5 ,060 ,924

PreKolesterolTotal &PostKolesterolTotal

Pair1

PreTrigliserida &PostTrigliserida

Pair2

N Correlation Sig.

Paired Samples Test

-18,88800 19,36923 8,66218 -42,93807 5,16207 -2,181 4 ,095

20,96000 25,08302 11,21747 -10,18469 52,10469 1,869 4 ,135

PreKolesterolTotal -PostKolesterolTotal

Pair1

PreTrigliserida -PostTrigliserida

Pair2

Mean Std. DeviationStd. Error

Mean Lower Upper

95% ConfidenceInterval of the

Difference

Paired Differences

t df Sig. (2-tailed)

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9. Paired t test kelompok perlakuan 1 prepost kolesterol total dan trigliserida


30,0080 5 5,43752 2,43173

39,7120 5 5,43791 2,43191

93,5200 5 37,30539 16,68348

74,8800 5 9,92809 4,43998

PreKolesterolTotal

PostKolesterolTotal

Pair1

PreTrigliserida

PostTrigliserida

Pair2


Mean


5 -,547 ,340

5 ,184 ,766


Pair1


Pair2

N Correlation Sig.

Paired Samples Test

-9,70400 9,56479 4,27750 -21,58026 2,17226 -2,269 4 ,086

18,64000 36,79121 16,45353 -27,04232 64,32232 1,133 4 ,321


Pair1


Pair2


Mean Lower Upper


Difference

Paired Differences


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10. Paired t test kelompok perlakuan 2 prepost kolesterol total dan trigliserida


38,9800 5 2,91297 1,30272

39,8480 5 12,68609 5,67339

105,1800 5 12,37122 5,53258

88,2200 5 8,05090 3,60047

PreKolesterolTotal

PostKolesterolTotal

Pair1

PreTrigliserida

PostTrigliserida

Pair2


Mean


5 ,096 ,878

5 -,175 ,779


Pair1


Pair2

N Correlation Sig.

Paired Samples Test

-,86800 12,74118 5,69803 -16,68827 14,95227 -,152 4 ,886

16,96000 15,89475 7,10835 -2,77594 36,69594 2,386 4 ,076


Pair1


Pair2


Mean Lower Upper


Difference

Paired Differences


67

11. Uji Wilcoxon pada kelompok perlakuan 3 kolesterol total

12. Paired t test kelompok perlakuan 3 prepost trigliserida

Ranks

1a 5,00 5,00

4b 2,50 10,00

0c

5

Negative Ranks

Positive Ranks

Ties

Total

PostKolesterolTotal -PreKolesterolTotal

N Mean Rank Sum of Ranks

PostKolesterolTotal < PreKolesterolTotala.

PostKolesterolTotal > PreKolesterolTotalb.

PostKolesterolTotal = PreKolesterolTotalc.

Test Statisticsb

-,674a

,500

Z

Asymp. Sig. (2-tailed)

PostKolesterol

Total -Pre

KolesterolTotal

Based on negative ranks.a.

Wilcoxon Signed Ranks Testb.


112,7000 5 11,03540 4,93518

96,6000 5 22,88613 10,23499

PreTrigliserida

PostTrigliserida

Pair1


Mean

68

Lampiran 5. Dokumentasi penelitian

Gambar a. Makroskopis kelompok kontrol. Tampak Normal dengan permukaan halus dan rata

Gambar b. Makroskopis kelompok perlakuan 1. Tampak Normal dengan permukaan halus dan rata

Gambar c. Makroskopis kelompok perlakuan 2. Tampak Normal dengan permukaan halus dan rata

Gambar d. Makroskopis kelompok perlakuan 3. Tampak Normal dengan permukaan halus dan rata

69

Gambar f. Perbesaran 400x. Mikroskopis ginjal. Lumen menutup ( ). Brush border(-) ( ).

Gambar e. Perbesaran 400x. Mikroskopis ginjal. Glomerulus ( ). Tubulus proximal( ).

Gambar g. Perbesaran 400x. Mikroskopis ginjal. Lumen menyempit ( ). Fokus radang ( ).

Gambar h. Perbesaran 400x. Mikroskopis ginjal. Perdarahan ( ). Fokus radang ( ).

70

Lampiran 6. Biodata mahasiswa

Identitas

Nama : Yurisal Akhmad Damy

NIM : G2A009137

Tempat/tanggal lahir : Semarang, 23 Juli 1992

Jenis Kelamin : Laki-laki

Alamat : Jln. Pamularsih Raya No. 56, Kec. Semarang Barat,

Kel. Salamanmloyo, Rt 02 / Rw 05. Kode Pos 50143

Nomor telpun : (024) 762 16 14

Nomor HP : 085 641 248 555

e-mail : [email protected] / [email protected]

Riwayat Pendidikan Formal

1. SD : SD. Hj. Isriati Tahun lulus : 2004

2. SMP : SMP Semesta Tahun lulus : 2007

3. SMA : SMA Negeri 1 Semarang Tahun lulus : 2009

4. FK UNDIP : Masuk tahun 2009

Keanggotaan Organisasi

1. Koor Humas Diponegoro Care Center Tahun : 2012 s/d 2014

2. Ketua BEM FK KM UNDIP Tahun : 2011 s/d 2012

Pengalaman penelitian

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DAFTAR PUSTAKA - Diponegoro University | Institutional ...eprints.undip.ac.id/44210/9/Yurizal_G2A009137_BabVIIIKTI.pdf · DAFTAR PUSTAKA 1. Oktaviani WD, ... dan Orang Tua dengan