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PLANTY- Objectives and Current Progress Dr. Sanjay Nene, Scientist, National Chemical Laboratory, Pune , INDIA Prof. Dr. Chlodwig Franz Head, Institute for Applied Botany & Pharmacognosy Vet.- med. University Vienna, AUSTRIA PLANTY Team members Prof. Dr. Chlodwig FRANZ, Austria Dr. Sanjay NENE, India Prof. Dr. Reinhold CARLE, Germany Dr. Petra BECKER, Netherland Dr. Massimo CASTELLARI, Spain Prof. Dr. Hüsnü Can BAŞER, Turkey Homepage: www.planty-project.net

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PLANTY- Objectives and Current Progress

Dr. Sanjay Nene, Scientist, National

Chemical Laboratory, Pune , INDIA

Prof. Dr. Chlodwig FranzHead, Institute for Applied Botany & Pharmacognosy Vet.-med. UniversityVienna, AUSTRIA

PLANTY Team members

• Prof. Dr. Chlodwig FRANZ, Austria• Dr. Sanjay NENE, India• Prof. Dr. Reinhold CARLE, Germany• Dr. Petra BECKER, Netherland• Dr. Massimo CASTELLARI, Spain• Prof. Dr. Hüsnü Can BAŞER, Turkey

Homepage: www.planty-project.net

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Project Objectives (I)

• To discuss about and to identify the most promising research fields concerning feed and food from plant-derived products and by-products in animal and human health.

• To bridge the gap between European and Indian research establishments and to find new and actual research topics and tendencies for the future

Project Objectives (II)

Establishing expert working groups to exchange their research experiences, to communicate, to build new networks in the field of feed security, feed additives, antimicrobials, functional compounds, dietary supplements all derived from plant origin. The planned NPP will support the Indian partner of the consortium in formulating suggestions for future research. The expert working groups will identify new research topics in this field also in Europe.

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The Kick-Off-Workshop was organised in order to:• Discuss findings of the existing projects• Disseminate and update research results of existing /

completed projects (e.g. SAFEWASTES, FEED-SEG, NAMASTE, PlantLIBRA)

• Define priorities for future research & innovation and of management strategies

• Include partners from the existing FP6/7 projects and representatives from other research projects in Europe and India, other experts and stakeholders and representatives from e.g. the European Food Safety Authority (EFSA)

The other 4 workshops will be organised in order to:

• present the future R&D expert groups outcomes• Identify new project ideas for FP7 and relevant

partnerships concerning the use of feed additives/antimicrobials/functional compounds/dietary supplements/recycling of residues and so on, all derived from plants

• Award selected high-quality project ideas

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PLANTY meetings

Meetings already held:• Kick off Meeting in Vienna (12th-13th October 2010)• First Meeting in Girona, Spain (23rd-24th May 2011)• Second Meeting in Antalya, Turkey (9th-10th September

2011)Future Meetings:• Third Meeting in Goa, India (6th-7th February 2012)• Fourth Meeting in Stuttgart, Germany (1st week of June

2012)• Final Meeting in Istanbul, Turkey in September 2012

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Pomegranate production in the World

World production of Pomegranate fruit is 1 million tonnes/pa

India is one of the leading country in pomegranate production. It produces 500,000 tonnes/y with more than 100,000 hectare under cultivation presently. Current exports are 5,000 tonnes/y (approx € 66 million.)

Spain produces 100,000 tonnes/y but exports 75,000 tonnes.

Turkey is another major producer24.05.2011

SOME EXPERIMENTAL RESULTS ON POMEGRANATE

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Proximate composition of Pomegranate fruit (wet basis)

Weight of

Pomegranate

50 kg % Composition of

fruit

Weight of arils 27.2 kg 54.4

Weight of pomace 5.83 kg 11.66

Weight of peel 22.16 kg 44.32

Weight of juice 20.83 kg 41.66

Processing of Pomegranate

Pomace

Dried at 50 C

Ground to 200 mesh in a ball mill

Packaging Under

Nitrogen

Juice

Clarified through 0.45 µm ceramic

membrane

Storedaseptically

Rind

Dried in a tray dryer at 50 C with

moisture content

< 5% w/w

Packed under Nitrogen

Ground to 200 mesh in a ball

mill

Flow chart for Processing of Pomegranate

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Preliminary Analytical Findings (I)

• Total dissolved solids in juice (Refractometer value) : 14.5% w/v

• Concentration of total phenolic compounds in juice (Folin Ciocalteau) is 3.21 mg/ml.

• Concentration on total reducing sugar in juice (DNSA) is 135.96 mg/ml.

• Protein concentration (Bradford method) in juice is 0.701 mg/ml.

Preliminary Analytical Findings (II)

• Methanolic extract of pomegranate peel yielded approx 152.21 mg/gram phenolic residues (as tannic acid)

• Hexane extract of pomegranate pomace yielded 202.1 mg/gram of extract

24.05.2011

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24.05.2011

Method/ Variable Concentration

Soxlhet Extraction of lipid from Pomegranate seed

20.7 g/100g seed

Neutral lipids in oil 90.9% w/w

Glycolipids in oil 4.2% w/w

Phospholipids in oil 4.9% w/w

Analysis of total lipid in Pomegranate seed

24.05.2011

Analysis of lipid profile in Pomegranate seedFatty acid Weight (%) of the sample

12:0 ND

14:0 ND

16:0 (palmitic acid) 9.31

18:0 (stearic acid) 6.89

18:1 (oleic acid) 15.21

18:2 (linoleic acid) 14.98

18:3 (α linolenic acid)

20:0 (arachidic acid) 2.62

20:1 (eicosenoic acid) 3.64

20:5 ND

18:3 (punicic acid) 47.31

ND = Not Detected

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Antioxidant activity of Pomegranate sample

Extraction of the samples: A: with 50% methanol to extract free antioxidants

B: with methanol/HCL to extract free and bound antioxidant

Test systems used:

• Total Phenolics (TP) mg caffeic acid (equivalents)/ g dry mat.

• DPPH bleaching test (DPPH) mg Trolox (equivalents)/ g dry matter of sample

• Fe-reduction test with TPTZ (FRAP) mg Trolox (equivalents)/ g dry matter of sample

24.05.2011

24.05.2011

Sample Extraction TP(mg/g)

DPPH(mg/g)

FRAP (mg/g)

PEEL A: MeOH 126 385 272

B: MeOH/HCl 143 386 194

SEED A: MeOH 9,2 18,0 14,6

B: MeOH/HCl 14,4 76,2 17,6

Anti-oxidant analysis of Pomegranate Peel and Seed Powder

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Proposed Experimental work in the 2 nd year:

• Studies on stabilization and encapsulation of pomegranate peel extracts

• Some preliminary poultry feed trials using pomegraneate fruit pomace

• Further studies on the possible use of pomegranate peel and other plant derived by-products

Details of Full Proposal submitted in November 2011 under KBBE.2012.2.3-01:

Feed production from food wasteCreating Value from Food Waste

(CREATIVEWASTE)With CREATIVEWASTE it is intended to:• recuperate and valorise food waste • enhance the food waste received from plant sources or

dairy industries to create high performance products to be used locally as an animal feed at a lower cost.

• define the most suitable food waste streams that may be used as feed production source and that are guaranteed with regards to safety. A respective matrix of data will be established as first step.

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Further Activities

• Intensified Researcher’s Exchange• Intensified Contact to Stakeholders• Searching / Checking for New Joint

Projects

• ...but under current administrative restrictions?

Advantages / Disadvantages

• Improving International R & D Contacts• Disseminating R & D Results• Generating New R & D Ideas • Establishing New Project Consortia

• Non-coordinated National Funding and Administration

• Lack of Personnel Costs (Europe)

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THANK YOU

Looking into the Future,

IT LOOKS GOOD!