鉄欠乏耐性イネ説明資料

資料 ２

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Distribution of CALCISOLS Based on WRB and the FAO/Unesco Soil Map of the World

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Fe

Fe

Fe

Fe

Fe

Fe

Fe

Fe

Fe

FeFe

Fe

Fe

Fe

FeFe

Fe

Fe

FeFe

Fe

FeFe

Fe

Fe

Fe

Fe

Fe

Fe

Fe

Fe

Fe

FeFe

FeFeFe

Fe

FeFe

Fe

Fe

FeFeFeFe

Fe

FeFe

Fe Fe

Fe

Fe

Fe

Fe

CO2H

OH

CO2HCO2H

OH

Mugineic acidN N

Fe

H

YS1

Strategy-II

㊪

NH

NH2

COOH COOH COOH

N

(NAS)

S- -L- (SAM)

SAM(SAMS)COOH

H3C S NH2

㌹(NAAT)

NH

O

COOH COOH COOH

N

3”-oxo acid

(DMAS)NH

OH

COOH COOH COOH

N

2’-

NH

OH

COOH COOH COOH

N

NH

OH

COOH COOH COOH

N

3-

NH

OH

COOH COOH COOH

N

NH

OH

COOH COOH COOH

N

3- -2’-

NH

OH

COOH COOH COOH

N

3-

OH

OH OHHO HO

HO

IDS3

IDS3IDS2

IDS2

HO

naat-B

-Fe+Fe

naat-A

Shoot

Root

Shoot

Root

naat-A

naat-B

B C

A

NP NPT II NT MCSLacZ 35P iGUS NT 35P HPT NT

RB LB

Ri ori RK2 ori

Hin dIII, Spe I, Not I (multicloning site)

NAAT genomic fragment 11kbNot I Not I

naat-Anaat-B

0

10

20

0

2

4

6

8

10(g) (g)Shoot Grain

C1 C6 C8 N1 N3 N8 N9 N17

Daysaftertransfertoanalkalinesoil(days)

(cm)

N1N3N8N9N17C1C6C8

85756555453520

40

60

80

100

A

B

C1 C6 C8 N1 N3 N8 N9 N17

NAAT(Takahashi et al., Nature Biotechnology 19:466-469, 2001)

A B

NAAT(Takahashi et al., Nature Biotechnology 19:466-469, 2001)

NAAT

NAATcontrol

control

NH OH

COOH COOHCOOH

N

DMA

OH

NH OH

COOH COOHCOOH

N

MAepiHMA

OH

NH OH

COOH COOHCOOH

NHO

NH OH

COOH COOHCOOH

NHO

epiHDMA

MAs

AdeAMP

ATPPRPPPPi

CH3 S CH2 CH2 COO-

CH2CHNH3+

Ade

R COO-CHNH3+

OHCH2

O

OH

CH2 AdeS

OPO3H-

CH2CH3 S

ATP

R COO-CO

ATP

ADP

H2O

HCOO-

O2

HPO42-O

OH OH

O

OH OH

O

OH OH

methionine

SAM

OH

CO2

NAD+NADH

NH NH2

COOH COOHCOOH

N

NA

NH O

COOH COOHCOOH

N

keto-form

IDI1

NAS

APRT

NAAT

IDS3 IDS3

Ids2

Ids2

SAMsynthetase

S CH2 OPO3H-CH2CHCH3 CH COOH

OH

Yang Cycle(Methionine Cycle)

CH3 S

CH3CH3 S CH2 CH2 COO-CO

S CH2 C=CH-O-CH2CH3 CO COOH

CH3 S CH2 CH2 COO-CHNH3+

OHS CH2 OPO3H-CHCH2CH3 CO C=

S CH2 OPO3H-CH2CH2CH3 CO CO

FDH

ムギネ酸類の生合成経路２

Ids3 genome 20.0 kb

HvNAS1 cDNANaat-A cDNAHvAprt cDNA

Ids3 5' 2.2kIds3 5' 2.2kIds3 5' 2.2k

Ids3 5' Ids3 5'

Ids3 5'Ids3 5'Ids3 5'

HvNAS1 cDNA Naat-A cDNA

Naat-A cDNAHvNAS1 cDNA HvAprt cDNA

HvNaat genome 11.0 kbHvNAS1 genome 13.0 kb1

6

5

4

3

2

7

8

Ids3 cDNANaat-A cDNA

HvNAS1 cDNA9

11

10

35Spro 0.8k

Ids6 genome 20.0 kb

12

HvNAS genome 13 kb HvNaat genome 11 kb14

鉄欠乏耐性イネ作成に使用したコンストラクト

（赤字は今回申請したもの）

35Spro 0.8k35Spro 0.8k

gHvNAS1-gHvNNAT

I3pNas-Naat-Aprt

gHvIDS3

I3pAPRT

gHvNAS1 gHvNAATvector control

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0

1

2

3

4

5

0

10

20

30

40

50

60

70

80

90

5月24日 6月21日 7月19日 8月16日

gHvNAS1

gHvNAS1-gHvNAAT

gHvIDS3

gHvNAAT

I3pAPRTI3pNasNaatAprt

vector control

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