Effects of UV-B on the growth of Vigna radiata ( 綠豆 ), Vigna angularis ( 紅豆 ) and Glycine max ( 大豆 ) ST. PAUL’S CO-EDUCATIONAL COLLEGE CHUI HO YIN PAUL

Effects of UV-B on the growth of Vigna radiata ( 綠豆 ), Vigna

angularis ( 紅豆 ) and Glycine max ( 大豆 )

ST. PAUL’S CO-EDUCATIONAL COLLEGECHUI HO YIN PAUL

CHAN YUN YIN CYRUSLEE WAI LAM BRYAN

Hey! Dear, I have a fabulous yield this year!!!

Dad! We may get a poor yield next

ten years!!!

WHY!?!?!

光禿禿?

Observations and facts

Ozone concentration decreases

– Higher amount of ultraviolet-B (UVB) radiation reaching the earth

– cause harmful effects to the biosphere

Literature ReviewThe effect of UVB on plants

Negative impacts of UVB on Glycine max:– Decrease the areas and the number of leaves

– Decrease height of the stems

– Decrease the photosynthetic rates and the evaporation rate

– Delay the growth process

Problem??

Will the yield of the crops be reduced???

Objectives

1. To find out the effect of UVB radiation on 3 types of plants (Vigna radiata 綠豆 , Vigna angularis 紅豆 and Glycine max 大豆 )

2. To find out which of these three plants has stronger resistance to the damaging effect of UVB radiation

Hypothesis

Under extra UVB:

The growth processes would be delayed

Fewer leaves would be developed

Shorter stems would be developed

Set-up 1BVigna angularis

Set-up 1CGlycine max

Set-up 1AVigna radiata

Set-up 2BVigna angularis

Set-up 2CGlycine max

Set-up 2AVigna radiata

Methodology

Set-up Type of seed Volume ofwater added

daily (cm³)

Number of seeds

Set-up 1 (control set-

up)

1 A Vigna radiata

綠豆84 40

1 B Vigna angularis

紅豆84 40

1 C Glycine max

大豆84 40

Set-up 2 2 A Vigna radiata

綠豆84 40

2 B Vigna angularis

紅豆84 40

2 C Glycine max

大豆84 40

Variable TableControlled variables Independent variables Dependent variables

Number of seeds used

Number of cotton layers used

Volume of water poured everyday

Types of plants

Illumination treatment

Number of leaves

Length of stems

Colour of leaves

Illumination treatment

Setup 1

7:00 18:00Sunlight

Setup 2

7:00 18:0013:00 16:00Sunlight

Sunlight + UV (300nm-400nm)

Sunlight

Result

Setup 1B Setup 1C Setup 1A

Setup 2B Setup 2C Setup 2A

The average number of leaves of Vigna radiata 綠豆with or without extra UVB

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

0 5 10 15 20 25

Day

Ave

rage

num

ber o

f lea

ves

withoutextra UVB

with extraUVB

The growth of Vigna radiata 綠豆with or without extraUVB

0

5

10

15

20

25

0 5 10 15 20 25 Day

The

leng

th o

f Vig

na r

adia

ta (

cm)

withoutextra UVB

with extraUVB

The average number of leaves of Vigna angularis 紅豆with and without extra UVB

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

0 5 10 15 20 25 Day

Ave

rage

num

ber

of le

aves

withoutextraUVB

with extraUVB

The growth of Vigna angularis 紅豆 with or withoutextra UVB

0

5

10

15

20

25

30

35

0 5 10 15 20 25 Day

The

leng

th o

f Vig

na a

ngul

aris

(cm

)

withoutextra UVB

with extraUVB

The average number of leaves of Glycine max 大豆with or without extra UVB

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0 5 10 15 20 25 Day

Ave

rage

num

ber o

f lea

ves without

extraUVB

withextraUVB

The growth of Glycine max 大豆 with or withoutextra UVB

0

5

10

15

20

25

30

0 5 10 15 20 25 Day

The

leng

th o

f Gly

cine

max

(cm

)

withoutextraUVB

withextraUVB

Colour of leaves

• Without extra UVB (Set-up 1):– green

• With extra UVB (Set-up 2):– green → yellow → brown →black (died)

CONCLUSION 1• Under extra UVB:

– Delayed germination

– Delayed foliation

– Fewer leaves developed

– Shorter stems developed

– Colour of the leaves changed from green to black

WHY??

Analysis

• Set-up 2– Green → Yellow → Brown →Black

• Maybe UVB blocks some nutrients from being absorbed by plants

– Slow down chlorophyll formation

– Cause the deficiency and poor growth

Further investigation• Measure the amounts of photodimer and photolyase

before and after UVB illumination

• Investigate the relationship between the UVB dosage and the amounts of photodimer formed

• Investigate the effect of UVB on the photosynthetic rate of plants

Which plant has the best resistance to

UVB???

The highest average number of leaves developed in theset-ups with and without extra UVB

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

Vigna radiata Vigna angularis Glycine max

Hig

hes

t av

erag

e n

um

ber

leav

es r

ecor

ded

without extraUVB

with extraUVB

10.7

0.6

Both Glycine max (大豆 ) & Vigna angularis(紅豆 ) had shown small

difference

The day of first foliation with and without extra UVB

0

2

4

6

8

10

12


Day

of

firs

t fo

liat

ion

without extra UVBwith extra UVB1

5

4

Vigna radiata綠豆 had the smallest difference

The day of germination with and without extra UVB

0

2

4

6

8

10

12


Day

of

germ

inat

ion

without extraUVBwith extra UVB1

53


The highest average height recorded in the set-ups with andwithout extra UVB

0

5

10

15

20

25

30

35

Vigna radiata Vignaangularis

Glycine max

Hig

hest

ave

rage

hei

ght r

ecor

ded

(cm

)

withoutextraUVBwith extraUVB

10.3

1311.6


The day at which the plants grown under extraUVB died

0

5

10

15

20

25


Day

that

the

seel

ding

die

d

with extra UVB

Vigna radiata ( 綠豆 ) in the set-up with UVB died latest among three set-ups.

CONCLUSION 2

• The growth of Vigna radiata(綠豆 )is least affected under extra UVB condition among the three plants

• Vigna radiata (綠豆 )has the best resistance to UVB among the three plants (Vigna radiata, Vigna angularis and Glycine max) studied

Further investigation• Investigate the effect of UVB dosage on the

growth of Vigna radiata (綠豆 )

• Investigate whether Vigna radiata (綠豆 )from different climatic regions show different resistance to UVB

In order to have a good harvest, is it more

beneficial for me to grow Vigna radiata?

I think…

THANK YOU!!!Please protect our

earth!!!!

REFERENCE1. 二氧化碳 , 臭氧 , 紫外輻射與農作物生產─氣象出版社

2. Elevated Ultraviolet (UV)-B Radiation and Agriculture. Springer Press.

3. Plants and UV-B─Responses to Environmental Change. Cambridge University Press.

4. Ozone crisis: the 15-year evolution of a sudden global emergency. John Wiley and Sons Press.

Websites5. http://earthobservatory.nasa.gov/Library/UVB/6.

http://www.bioon.com/biology/Class45/molecular/200406/53376.html

• 7. http://www.ciesin.org/docs/011-467/011-467.html8. http://www.mountcarmelhealth.com/healthinfo/Adult/skin/ultravio.shtml

9. http://uvb.nrel.colostate.edu/UVB/plant_impact/intro.html10. http://www.epa.gov/safewater/mdbp/pdf/alter/chapt_8.pdf11. http://www.people.com.cn/BIG5/huanbao/56/20010913/559617.html

12. http://www.npcc.gov.tw/nm-research/doctors/Concise_NM/%E7%AC%AC%E5%8D%81%E4%BA%8C%E7%AB%A0%20%20%20%E9%9B%BB%E9%9B%A2%E8%BC%BB%E5%B0%84%E7%9A%84%E7%94%9F%E7%89%A9%E5%AD%B8%E6%95%88%E6%87%89.htm

13. http://www.stut.edu.tw/epc/epc5/06(final).ppt

Comparison of the highest average number of leaves between the set-ups with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)

Plant used Set-ups The highest average number of

leaves recorded

Difference between two

set-ups

Vigna radiata 1A (without UVB)

1.7 1

2A (with UVB) 0.7

Vigna angularis

1B (without UVB)

1.5 0.7

2B (with UVB) 0.8

Glycine max 1C (without UVB)

1.3 0.6

2C (with UVB) 0.7

Difference of the day of first foliation between the set-up with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)

Plant used Set-ups Day of the first

foliation recorded

Difference between two set-ups

Vigna radiata - UVB 10 1+ UVB 11

Vigna angularis

- UVB 4 5

+ UVB 9

Glycine max - UVB 6 4

+ UVB 10

Difference of day of germination between the set-ups with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)

Plant used Set-ups Day of germination

Difference between two

set-ups


10 12A (with UVB) 11

Vigna angularis

1B (without UVB)

4 5

2B (with UVB) 9


6 3

2C (with UVB) 9

Comparison of the highest average height recorded between the set-ups with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)

Plant used Set-ups Highest average height

recorded

Difference between the two set-ups


21 10.32A (with UVB) 9.7

Vigna angularis

1B (without UVB)

29 13

2B (with UVB) 16


25 11.6

2C (with UVB) 13.4

The day of in which the plants grown under UVB died

Plant used Set-ups Day of death

Vigna radiata 2A (with UVB) 23

Vigna angularis 2B (with UVB) 22

Glycine max 2C (with UVB) 19

“Heating effect” of UVB is negligible……..

Initial temperature inside the acrylic box with switched-on UV lamp ( )℃

Temperature inside the acrylic box with switched on UV lamp after 3 hours ( )℃

26 26.5

The acrylic box

Documents

Effects of UV-B on the growth of Vigna radiata ( 綠豆 ), Vigna angularis ( 紅豆 ) and Glycine max ( 大豆 ) ST. PAUL’S CO-EDUCATIONAL COLLEGE CHUI HO YIN PAUL