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FUSION SOLO S

S A L E S M A N U A L

VILBER is pleased to announce the release of a new compact personal imager to enhance our current Western Blot and fluorescent documentation product portfolio, Fusion Solo S. This Sales Manual is intended to provide a thorough collection of product information for sales and training support.

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FUSION SOLO S SALES MANUAL

Agenda

Section 1: Product Description ............................................................................................... 3

1.1 General Overview ............................................................................................................. 3

1.1 Press Release .................................................................................................................... 4

1.2 Key features and benefits ................................................................................................ 5

1.3 Who are Solo S’ target customers? .................................................................................. 6

1.4 Product Positioning Overview .......................................................................................... 6

1.5 Performance data – Compared to film ............................................................................ 7

1.6 Performance data – Compared to competitors ............................................................. 12

1.7 Applications .................................................................................................................... 14

Section 2: Fusion Solo S in details ........................................................................................ 15

2.1 Product specifications .................................................................................................... 15

2.2 Options and accessories ................................................................................................. 19

2.2 Ordering information ..................................................................................................... 20

2.3 Computer system requirement ...................................................................................... 22

2.4 Qualification and CFR21 ................................................................................................. 22

Section 3: Frequently asked question .................................................................................. 24

About VILBER LOURMAT ...................................................................................................... 26

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Section 1: Product Description

1.1 General Overview

Vilber Lourmat is pleased to announce the release of a new compact personal imager to enhance our current Western Blot documentation and fluorescent product portfolio, Fusion Solo S. Fusion Solo S is designed to address the constraints of a precious lab commodity, available bench space. While the dimensions are compact, the specifications are not. Solo S boasts a Sony unrivalled high 74% Quantum Efficiency sensor, 3.2 megapixels camera with our custom made unrivalled F:0.84 lens, 4 position filter wheel, LED epi white lights, a built‐in innovative Applications Pad container which can welcome any kind of illumination from UV to IR. The Solo S is impressively housed in a design and size that is reminiscent of a high-tech medical design. The current imaging market provides unique challenges. Solo S was designed with the consumer in mind, offering a fresh and innovative design while maintaining the high standards in image quality that customers have come to expect from Vilber Lourmat. This Sales Manual is intended to provide a thorough collection of product information generated from the course of product conception, development, implementation and testing.

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1.1 Press Release

SOLO S™ PERSONAL BLOT and GEL IMAGER NOW AVAILABLE FROM VILBER LOURMAT September 15, 2014- Vilber Lourmat, Paris, France. Vilber Lourmat, a leading provider of innovative bioimaging solutions for the life science research market, announced today that it has begun shipment of SOLO S™, its newest Western blot and gel imaging system. Designed to take advantage of the burgeoning global market for personal laboratory instruments, the SOLO S personal gel imager combines extremely high sensitivity camera with advanced imaging technology and an innovative Application Pad container, all in a uniquely compact footprint. SOLO S’s scientific-grade sensor and innovative optical design provides the flexibility critical to imaging chemiluminescent Western blot and the growing list of fluorescent labels found in a typical laboratory. SOLO S’s integrated transillumination and epi illumination sources combined with its four position filter wheel are designed for exciting and imaging a wide variety of UV, visible and IR fluorescent labels commonly used in blot and stained DNA and protein gels. SOLO S also excels at imaging white light applications such as coomassie and silver stained protein gels. SOLO S was designed for maximum ease of use. From its simple installation to its intuitive user interface, this system is plug-and-play. “Vilber Lourmat has long been a market leader in high end chemiluminescence and fluorescence imaging workstations,” stated Pierre Oleggini, C.E.O. “With SOLO S, we have taken our advanced technology and made it simple and intuitive to use and integrated all system components into a compact footprint. SOLO S provides a powerful yet cost effective solution that we believe will enable more labs worldwide to adopt digital blot documentation systems.” About Vilber Lourmat Founded in 1954 and with over 20,000 systems sold worldwide, Vilber Lourmat is the leading European provider of molecular imaging system, analysis software and UV fluorescence equipment for the life science research and the drug discovery sector. VILBER has pioneered the post electrophoresis market and introduced breakthrough products such as stand-alone gel-documentation, Bio-1D imaging software, Super-Bright UV technology, dedicated chemiluminescence imaging system and 3D approach to 1D gel analysis. Through a network of owned subsidiary offices and local distributors located in over 60 countries around the world, VILBER offers a broad range of imaging products for the research. Our customers include pharmaceutical and biotechnology companies as well as universities, medical centers, government research institutes and agencies worldwide. More information on Vilber Lourmat can be found at the Company’s website www.vilber.de

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1.2 Key features and benefits

Compact Design : industry leading dimension, requires minimal bench space

Aesthetically pleasing. High tech design. Unique look

Scientific Grade camera. Super sensitivity and extremely high level of resolution

Sony based high QE camera sensor: extremely high sensitivity with the necessary dynamic range to distinguish between subtle signal variations. Ability to image low intensity samples

High Dynamic range. Allows for quantitative analysis

Pre-calibrated focus for all defined sample height. Easy and convenient adjustment of lens settings

White LED lights / Bright illumination: allows for Western blot molecular weight imaging and other visible samples that require lighting from above

Intuitive software workflow

Great system for personal use or a core lab

4‐position filter wheel: allows for dye flexibility of different fluorescent stains. Easy filter selection

Light safety switch override for safety and for preparative work when the door is open

Cost effective: ability to purchase an high end gel and blot imaging system with analysis quality images

No assembly required : Out of the box, ready to go

Casing Light-tight high tech design

Innovative Application Pad container. Allows a wide variety of UV, visible and IR fluorescent imaging capabilities.

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1.3 Who are Solo S’ target customers?

Current Imaging customers

Ones that share a Core system with other lab(s)

Ones that do not have space for a full system

Ones that have limited budget for blot imaging Key Customer Profile:

Individual labs that want a simple gel documentation system that is compact enough to fit on their bench top.

These individuals want a blot and gel imager with performance superior to film and to any other gel imagers.

Ease of set up and operation and time to get an image are key.

1.4 Product Positioning Overview

When FX, when Solo S? The FX system is a high-end fully motorized system. The Fusion Solo S has a manual

filter wheel and manual lens and is designed to be a very cost effective options for the laboratory with budget constraint.

When DARQ 5S and when DARQ 7? The DARQ 7 camera is an high-end camera ideal for any type of application, any type

of exposure time The DARQ 5S camera is ideal for most Western blot and fluorescence application for

which exposure time does not exceed 15 minutes.

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1.5 Performance data – Compared to film

Film is the main Fusion competitor. People like it because of its sensitivity.

Despite its sensitivity, film has several disadvantages:

Multiple film exposure could be required to get appropriate signal

Film dynamic range is not sufficient for quantification

Film required to be scanned to get a digitalized image ready for quantification software

Film is a costly consumable

Film requires a separate dark room and a film developer

Films requires chemicals for film development that need to be disposed separately and monitored

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In comparisons to films, several aspects need to be considered:

Sensitivity

Signal to noise ratio

Linearity

Dynamic range

Quantification of the proteins Sensitivity will affect the amount of light that the system picks up and converts into your photo. Sensitivity is about the quantity of signal that can be collected by the system within a defined period of time.

The noise is the background baseline of the image. The imaging system can only collect the signal above this noise base. It should then be maintained to the lowest possible level.

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The linearity describes the relationship between the detected signal and the sample quantity. The film has a reputation not to be linear. As a consequence, signal cannot be measured from a film.

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Dynamic range refers to the possibility to have on the same image both the very faint bands and the very bright band without saturation of the bright bands. If the dynamic is poor, the system cannot detect the faint band without oversaturated the bright bands.

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Performance compared to film: Sensitivity

The Fusion systems sensitivity is at least equal as the sensitivity of film.

Film Fusion Solo S

Sensitivity

Performance compared to film: Noise / Signal to noise

The film could be highly noisy. On the opposite, the Fusion has a supreme stable low noise floor level.

Film Fusion Solo S

Noise / Signal to noise

Performance compared to film: linearity

The Fusion system use a scientific CCD camera designed to provide the best possible linearity.

Film Fusion Solo S

Linearity None

Performance compared to film: Dynamic rane

The Fusion system can easily detect the faintest bands without signal oversaturation of the highest bands.

Film Fusion Solo S

Dynamic range

Performance compared to film: Ability to produce quantification ready images

Film Fusion Solo S

Image quantification None applicable

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1.6 Performance data – Compared to competitors

Main competitors for chemiluminescence imaging are:

Biorad – ChemiDoc Touch / ChemiDoc MP / ChemiDoc XR systems

GE – Amersham 600, LAS 500 Outsiders:

UVP (ChemiDoc-It)

Alpha Innotech / Protein Simple, now Bio Techne (FluorChem)

Syngene (Gbox) Biorad is the first name to come into the mind of the customers regarding Western blot imaging. The Vilber’s Fusion range plays in the high end and high performance part of the market. No other system provides higher performance as the Fusion systems.

Fig 2: Comparative low light images taken with Fusion versus competitive system with comparable imaging settings. The images are displayed with same relative intensity scaling.

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Fig5: Competitive system. The faint signal cannot stand out from the surrounding noise level

Fig6: The Fusion combined with the High Sensitivity Reading (HSR) technology provide a better signal to noise and more bands are detected. DARQ camera, cooling at -42°c.

Sensitivity / Noise / Image dynamic

Competitor Fusion Solo S

Sensitivity

Signal to noise

Dynamic range

Application coverage / Ease of use

Competitor Fusion Solo S

Application coverage

Ease of use

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1.7 Applications

Chemiluminescence

Bioluminescence

Visible imaging

UV fluorescence

Multiplexing fluorescence Choice of visible RGB, NIR or IR excitation source Fluorescent Gels • Built‐in 4 position filter wheel allows for easy changing of filters. • F590 interferential filter, optimal for Ethidium Bromide • Additional filters available Colorimetric Protein Gels • White-light Pad or White light conversion screen allows for colorimetric samples imaging • Both UV or white light can be used as light source

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Section 2: Fusion Solo S in details

2.1 Product specifications

Darkroom

SmartCab intelligent darkroom technology:

Software control of the lighting

Automatic white light intensity adjustment according to the sample position

Automatic filter wheel position reconnaissance

Precalibrated focus for all defined sample height

Fig7: Fusion Solo S cabinet: empty PadBox configuration.

PadBox multimodal container

Roll-out multimodal pad container

Could accommodate our UV, blue, white light or Spectra pad or your own hardware such as heater, cooler, electrophoresis tank, special light source etc.

Power socket inside the darkroom, switched on and off through the software

Fig2: The Fusion Solo S PadBox can easily integrate one of the several available Application Pad.

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PadConnect technology

Automatic detection of the application-specific pad

Imaging and software options adjusted accordingly

Integration with the AppsStudio application library

Fig8: The PadConnect technology allows an automatic recognition of the installed Application Pad.

Darkroom features

5 trays positions for Western blot samples

4 positions filter wheel

Led based white light EPI

Aluminum, steel & stainless steel cabinet

Solid light proof cabinet with security locker

Light safety switch override for safety and to allow for preparative work when the door is open

Small size to fit on any bench space

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Detector

Choice between the DARQ 7 and the DARQ 5S camera

DARQ 7

Scientific grade CCD camera Grade 0, zero defect KAI4022sensor Image resolution: 10 megapixels Native resolution: 2048x2048 1.3 inch sensor -67° C maximum cooling differential from ambient -42°C absolute and regulated cooling via four stages Peltier thermoelectric cooler. High Sensitivity reading (HSR) technology USB connection

DARQ 5S

Scientific grade CCD camera Grade 0, zero defect ICX694 sensor Image resolution: 9 megapixels Native resolution: 2100x1548 1 inch sensor -55° C maximum cooling differential from ambient -30°C absolute and regulated cooling via three stages Peltier thermoelectric cooler. High Sensitivity reading (HSR) technology USB connection

Fig9: The Darq7 sensor has a supersized CCD format which allows extremely dim light collection.

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Optical lens

Proprietary V.084 lens Aperture F: 0.84 Minimum focusing distance: 24cm Aspheric elements to deliver consistently sharp images out of the field of view Manual adjustment

Fig10: Our V.O84 lens has unrivalled sensitivity with an aperture of f/0.84 and allows the sample to be at 25cm distance from the camera.

Estimated field of view

DARQ 7 (mm)

Tray 1: 100x100 Tray 2: 110x110 Tray 3: 140x140 Tray 4: 160x160 Pad level: 190x190 PadBox level: 210x210

DARQ 5S (mm)

Tray 1: 85x70 Tray 2: 100x80 Tray 3: 120x95 Tray 4: 135x110 Pad level: 160x120 PadBox level: 180x140

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Software

Fusion-Capt v16.10 software for image acquisition and image analysis for the Solo 4S:

Autoexposure

Apps Studio

3D Dynamic scan Vision-Capt v16.10 software for image acquisition and image analysis for the Solo 3S:

Autoexposure

Apps Studio

3D Dynamic scan

Fig11: The Apps Studio contains more than 40 different imaging protocols for your blot, gel and other bioluminescence sample.

2.2 Options and accessories

Application-Pad

UV-Pad

Super-Bright-Pad

Sky-Pad: LED blue light transilluminator, 470nm

White-Light- Pad: LED white light transilluminator

Spectra-Pad RGB: Red, Green and blue EPI light module

Spectra-Pad IR-RG: IR, NIR and Green EPI light module [To be confirmed] All Application Pad except Spectra Pad can be used as a standalone illumination device outside the Fusion Solo S system, with an optional plug adaptor.

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Fig12: Several different Application-Pad can be easily inserted or removed inside the Fusion Solo S Filters

F-590 interference filter, suitable for documentation of Ethidium Bromide and most

stained DNA/RNA agarose and acrylamide gels

F-535 Y

F-565

F595 Y

F655

F695

F740 Y

F820 Conversion screen

FC-26WL

FC-26Blue

2.2 Ordering information

Fusion Solo S

Proprietary V.0.84 lens. F: 0.84 lens CN-Solo S darkroom with LED EPI white light No Application Pad / No emission filter included FUSION-SOLO.4S.WL # 1211 7600 1 > DARQ 7 camera Fusion-Capt software FUSION-SOLO.3S.WL # 1211 7100 1 > DARQ 5S camera Vision-Capt software

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Options

TFS-20.M UV-Pad 20M EU # 2871 2002 1 > UV 312nm – Filter size 20x20cm TFS-26.M UV-Pad 26M EU # 2871 2602 1 > UV 312nm – Filter size 21x26cm TFS-26.MX Super-Bright-Pad 26M 230V EU # 2871 2600 1 > Super-Bright UV 312nm – Filter size 21x26cm TFS-20.Sky-Pad EU # 2871 2008 1 > Blue 470nm - LED - Filter size 20x20cm TFS-20.W White-Pad EU # 2871 2009 1 > White light (visible light) - LED - Filter size 20x20cm FC-26.WL UV/WL conversion screen # 9120 2002 1 > UV to white light conversion screen FC-26.BLEU UV/BLUE conversion screen # 9120 2003 1 > UV to blue light conversion screen FC-25.WL SkyLight/WL conversion screen # 9120 2004 1 > Blue to white light conversion screen F-535Y M58 Narrow band filter Y2 # 1710 4180 1 F-565 M58 Narrow band filter Qdot # 1710 4160 1 F-590 M58 Large band filter # 1710 4113 1 F-595Y M58 Narrow band filter Y3 # 1710 4181 1 F-655 M58 Narrow band filter Qdot # 1710 4161 1 F-695 M58 Narrow band filter Y5 # 1710 4182 1 F-710 M58 Narrow band filter Qdot # 1710 4162 1 F-740NIR M58 Narrow band filter # 1710 4184 1 F-820IR M58 Narrow band filter # 1710 4183 1

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2.3 Computer system requirement

Minimum requirement

Main board Desktop computer with minimum Intel 5 series chipset board. Intel processor Do not use chipset Z68

Processor Intel Core I5 minimum – Intel CoreI7 recommended Cache size L3: 6 MB

Ram

8 Gb and above (DDR2-800 recommended)

Hard disk

250 Gb and above At least 10Gb free disk space least in order to allow software installation and image storage

Monitor / Video card

1280x1024/ 16 million color mode (24-bit). Upper resolutions supported.

Operating system

Microsoft Windows 8 (32 or 64-bit) Microsoft Windows 7 SP1 (and upper) (32 or 64-bit)

USB Port At least two USB2 ports available in the computer rear side

The DARQ 5S camera is not compatible with USB3 port.

2.4 Qualification and CFR21

IQ-OQ template can be supplied with the Fusion Solo S system; CFR21Part 11 module can be supplied with the Fusion Solo S system 2.5 Technical Requirements (electrical, safety, approvals, etc.) Electrical: • 100v or 220v • 50 / 60 Hz Space • Solo S should be placed on a stable benchtop that can support 40kg safely.

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• Temperature range: 10- 30°C • Relative Humidity Range: 50‐70% Weight: 27 kg to be confirmed. Dimensions: to be confirmed cm (WxDxH) Power consumption: 150 watt Certifications: CE Warranty Policy: Two year warranty on parts and labor

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Section 3: Frequently asked questions This list of questions are compiled from beta testing sites and demos performed. Short answers are also provided: • What kind of camera is in Solo S? A scientific grade cooled CCD camera designed for extreme low light application. The Darq7 camera has a Kodak sensor while the Darq 5S camera has a Sony sensor. • How do I control the camera? Solo S is controlled through the Capt software. Fusion-Capt for the Solo 4S and Vision-Capt for the Solo 3S. Both the camera and the darkroom are connected through the USB port to the computer. • If I use the same size blot, will the system remember my settings? The lens has a precalibrated focus for all defined sample height. Camera settings will return to / stay at last known configurations unless they are changed even after rebooting the system. You can save your own settings in a separate application protocol. • What’s the field of view? The full of view depends on the Solo S camera. It could reach 21x21 cm at the Pad container level. • Does the UV box turn off for safety? Yes, the UV light shuts off after each acquisition, and there is also a UV safety switch that turns the UV light when the door is opened. The user has the ability to override this switch for preparative work while door is open. In this case, eyes and skin should be protected from the UV. • Can we image gels stained with dyes other than EtBr? Yes, you should be able to image any dye that is UV 312 / 365 / Blue / Green / NIR or IR excitable. Solo S has a 4‐position filter wheel that allows for easy changing of filters. Filters are not included and can be purchased from Vilber Lourmat. • I typically use SYBR Green and SYBR Safe, can I image that in Solo S? Yes. Solo S comes optionally with a UV or a Blue Pad for the excitation of the SYBER Green and of the SYBR Safe. • Can we image protein gels? Yes, we have either a White Pad with LED uniform light or a white light conversion screen that can be used for imaging visible dyes. • Can we image stained free gels? Yes, we have either a UV-Pad designed for Stained Free as well as for the most common DNA/RNA dyes. Our Apps Studio provide imaging application protocol for the stain free applications, including tryptophan activation.

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• Can I image a silver‐stained gel? Yes, the optional white light conversion plate sits on the UV transilluminator. The sample tray holding your gel can be placed on top of the plate. Both the UV and white lights are suitable light sources. • Can I image molecular weight marker from my Western blot? The white LED lights will provide bright illumination for molecular weight blot marker. • What file format is my image saved in? The software will automatically save the image in a Compatibility Plus 16-bit file format for easy sharing with other Microsoft Windows applications. • Are the images 300 DPI for publication? Yes, our 16-bit format is 300dpi. • Is the transilluminator removable? Yes, the optional UV-Pad is easily removable without any tool. It could then be used next to your cabinet as a stand-alone transilluminator. • Does the system come with Bio-1D Analysis Software? No, this software is optional and can be purchased from Vilber Lourmat • What software comes with Solo S? The imager comes with Capt Software with easy to adjust exposure time, light selection, image acquisition, saving and printing. This software is designed for fast and easy image capture.

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About VILBER LOURMAT Founded in 1954 and with over 20,000 systems sold worldwide, Vilber Lourmat is the leading European provider of molecular imaging system, analysis software and UV fluorescence equipment for the life science research and the drug discovery sector. VILBER has pioneered the post electrophoresis market and introduced breakthrough products such as stand-alone gel-documentation, Bio-1D imaging software, Super-Bright UV technology, dedicated chemiluminescence imaging system and 3D approach to 1D gel analysis. Through a network of owned subsidiary offices and local distributors located in over 60 countries around the world, VILBER offers a broad range of imaging products for the research. Our customers include pharmaceutical and biotechnology companies as well as universities, medical centers, government research institutes and agencies worldwide. More information on Vilber Lourmat can be found at the Company’s website www.vilber.de