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Gambar lampiran 1. Jenis leukosit. a. Monosit, b. Basofil, c.
Eosinofil, d. Netrofil, e. Limfosit dengan
perbesaran mikroskop 1600X
a
b d
e
c
57
Alur Kerja
A. Perhitungan Jumlah Total Leukosit
Tahap I Tahap II
(Pengambilan sampel darah) (Sampel dengan Pipet Leukosit)
Tahap III Tahap IV
(Pencampuran Sampel dengan (Homogenisasi Darah dan Turk)
Larutan Turk)
58
Tahap V Tahap VI
(Peletakan Sampel (Pengamatan dengan Mikroskop)
pada Haemocitometer)
Gambar lampiran 2. Alur kerja pembuatan preparat untuk
menghitung jumlah total leukosit
B. Jenis Leukosit
Tahap I Tahap II
(Pengambilan Sampel Darah) (Sampel darah Pada Kaca Preparat)
59
Tahap III Tahap IV
(Pembuatan Darah Apus) (Sampel Preparat)
Tahap V Tahap VI
(Pewarnssn) (Pemberia Larutan Buffer)
Tahap VII Tahap VIII
(Pembilasan dengan Aquadest) (PengamatanMikroskop
Perbesaran 1600x)
Gambar lampiran 3. Alur kerja pembuatan preparat untuk
menghitung jenis-jenis leukosit
60
Lampiran 4. Jumlah Total Leukosit
Tests of Normality
Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statisti
c df Sig.
Statisti
c df Sig.
Jumlah Total
Leukosit
Vitamin E
tokotrienol .269 4 . .823 4 .150
Vitamin E
tokotrienol +
Asam
ascorbat
.273 4 . .864 4 .277
Kontrol .263 4 . .866 4 .283
a. Lilliefors Significance
Correction
Uji RAL menggunakan Uji OneWay Anova
Between-Subjects Factors
Value Label N
Perlakuan 1 Vitamin E
tokotrienol 4
2 Vitamin E
tokotrienol
+ Asam
ascorbat
4
3 Kontrol 4
61
Descriptive Statistics
Dependent Variable:Jumlah Total Leukosit
Perlakuan Mean
Std.
Deviation N
Vitamin E tokotrienol 6783.25 2484.012 4
Vitamin E tokotrienol +
Asam ascorbat 7916.25 3725.932 4
Kontrol 5045.00 16.793 4
Total 6581.50 2643.893 12
Levene's Test of Equality of Error
Variancesa
Dependent Variable:Jumlah Total
Leukosit
F df1 df2 Sig.
4.736 2 9 .039
Tests the null hypothesis that the error
variance of the dependent variable is
equal across groups.
a. Design: Perlakuan
Tests of Between-Subjects Effects
Dependent Variable:Jumlah Total Leukosit
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 5.365E8a 3 1.788E8 26.755 .000
Perlakuan 5.365E8 3 1.788E8 26.755 .000
Error 6.016E7 9 6684388.389
Total 5.967E8 12
a. R Squared = .899 (Adjusted R Squared = .866)
62
Post Hoc Tests
Multiple Comparisons
Jumlah Total
Leukosit
Games-Howell
(I) Perlakuan
(J)
Perlakuan
Mean
Difference
(I-J)
Std.
Error Sig.
95% Confidence
Interval
Lower
Bound
Upper
Bound
Vitamin E
tokotrienol
Vitamin E
tokotrienol +
Asam
ascorbat
-1133.00 2239.022 .872 -8306.89 6040.89
Kontrol 1738.25 1242.034 .444 -3451.55 6928.05
Vitamin E
tokotrienol +
Asam ascorbat
Vitamin E
tokotrienol 1133.00 2239.022 .872 -6040.89 8306.89
Kontrol 2871.25 1862.985 .391 -4913.43 10655.93
Kontrol Vitamin E
tokotrienol -1738.25 1242.034 .444 -6928.05 3451.55
Vitamin E
tokotrienol +
Asam
ascorbat
-2871.25 1862.985 .391 -10655.93 4913.43
Based on observed means.
The error term is Mean Square(Error)
= 6684388.389.
63
Estimated Marginal Means
Perlakuan
Dependent Variable:Jumlah Total Leukosit
Perlakuan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
Vitamin E tokotrienol 6.783E3 1292.709 3858.939 9707.561
Vitamin E tokotrienol +
Asam ascorbat 7.916E3 1292.709 4991.939 10840.561
Kontrol 5.045E3 1292.709 2120.689 7969.311
64
Lampiran 5. Jenis-Jenis Leukosit
NEUTROFIL
Tests of Normality
Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statisti
c df Sig.
Statisti
c df Sig.
Neutrof
il
Vitamin E
tokotrienol .318 4 . .843 4 .205
Vitamin E
tokotrienol + Asam
ascorbat
.355 4 . .773 4 .062
Kontrol .333 4 . .783 4 .075
a. Lilliefors Significance Correction
Uji RAL menggunakan Uji OneWay Anova
Between-Subjects Factors
Value Label N
Perlakuan 1 Vitamin E
tokotrienol 4
2 Vitamin E
tokotrienol
+ Asam
ascorbat
4
3 Kontrol 4
Descriptive Statistics
65
Dependent Variable:Neutrofil
Perlakuan Mean
Std.
Deviation N
Vitamin E tokotrienol 48.2500 14.16905 4
Vitamin E tokotrienol +
Asam ascorbat 50.8325 15.66604 4
Kontrol 34.0850 4.36535 4
Total 44.3892 13.63859 12
Levene's Test of Equality of Error
Variancesa
Dependent Variable:Neutrofil
F df1 df2 Sig.
1.855 2 9 .212
Tests the null hypothesis that the error
variance of the dependent variable is
equal across groups.
a. Design: Perlakuan
Tests of Between-Subjects Effects
Dependent Variable:Neutrofil
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 24295.171a 3 8098.390 52.220 .000
Perlakuan 24295.171 3 8098.390 52.220 .000
Error 1395.729 9 155.081
Total 25690.900 12
a. R Squared = .946 (Adjusted R Squared = .928)
66
Estimated Marginal Means
Perlakuan
Dependent Variable:Neutrofil
Perlakuan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
Vitamin E tokotrienol 48.250 6.227 34.165 62.335
Vitamin E tokotrienol +
Asam ascorbat 50.832 6.227 36.747 64.918
Kontrol 34.085 6.227 20.000 48.170
Post Hoc Tests
Multiple Comparisons
Dependent
Variable:Neutrofil
(I)
Perlakuan (J) Perlakuan
Mean
Differe
nce (I-
J)
Std.
Error Sig.
95% Confidence
Interval
Lower
Bound
Upper
Bound
LSD Vitamin E
tokotrienol
Vitamin E
tokotrienol +
Asam
ascorbat
-
2.582
5
8.805
71 .776 -22.5024 17.3374
Kontrol 14.16
50
8.805
71 .142 -5.7549 34.0849
Vitamin E
tokotrienol
+ Asam
ascorbat
Vitamin E
tokotrienol
2.582
5
8.805
71 .776 -17.3374 22.5024
Kontrol 16.74
75
8.805
71 .090 -3.1724 36.6674
67
Kontrol Vitamin E
tokotrienol
-
14.16
50
8.805
71 .142 -34.0849 5.7549
Vitamin E
tokotrienol +
Asam
ascorbat
-
16.74
75
8.805
71 .090 -36.6674 3.1724
Based on observed means.
The error term is Mean
Square(Error) = 155.081.
Homogeneous Subsets
Neutrofil
Perlakuan N
Subset
1
Duncana Kontrol 4 34.0850
Vitamin E tokotrienol 4 48.2500
Vitamin E tokotrienol +
Asam ascorbat 4 50.8325
Sig. .102
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 155.081.
a. Uses Harmonic Mean Sample Size = 4.000.
68
EOSINOFIL
Tests of Normality
Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statist
ic df Sig.
Statist
ic df Sig.
Eosino
fil
Vitamin E
tokotrienol .298 4 . .927 4 .574
Vitamin E
tokotrienol +
Asam ascorbat
.185 4 . .973 4 .857
Kontrol .298 4 . .849 4 .222
a. Lilliefors Significance
Correction
RAL menggunakan Uji OneWay Anova
Between-Subjects Factors
Value Label N
Perlakuan 1 Vitamin E
tokotrienol 4
2 Vitamin E
tokotrienol
+ Asam
ascorbat
4
3 Kontrol 4
69
Descriptive Statistics
Dependent Variable:Eosinofil
Perlakuan Mean
Std.
Deviation N
Vitamin E tokotrienol 2.9175 .68840 4
Vitamin E tokotrienol +
Asam ascorbat 2.5000 1.03888 4
Kontrol 3.2525 .50056 4
Total 2.8900 .77155 12
Levene's Test of Equality of Error
Variancesa
Dependent Variable:Eosinofil
F df1 df2 Sig.
1.759 2 9 .227
Tests the null hypothesis that the error
variance of the dependent variable is
equal across groups.
a. Design: Perlakuan
Tests of Between-Subjects Effects
Dependent Variable:Eosinofil
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 101.362a 3 33.787 56.196 .000
Perlakuan 101.362 3 33.787 56.196 .000
Error 5.411 9 .601
Total 106.773 12
a. R Squared = .949 (Adjusted R Squared = .932)
70
Estimated Marginal Means
Perlakuan
Dependent Variable:Eosinofil
Perlakuan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
Vitamin E tokotrienol 2.918 .388 2.040 3.795
Vitamin E tokotrienol +
Asam ascorbat 2.500 .388 1.623 3.377
Kontrol 3.252 .388 2.375 4.130
Post Hoc Tests
Multiple Comparisons
Dependent
Variable:Eosinofil
(I) Perlakuan (J) Perlakuan
Mean
Differe
nce (I-
J)
Std.
Error Sig.
95% Confidence
Interval
Lower
Bound
Upper
Bound
LSD Vitamin E
tokotrienol
Vitamin E
tokotrienol +
Asam
ascorbat
.4175 .5482
9 .466 -.8228 1.6578
Kontrol -.3350
.5482
9 .556 -1.5753 .9053
Vitamin E
tokotrienol +
Asam
ascorbat
Vitamin E
tokotrienol -.4175
.5482
9 .466 -1.6578 .8228
Kontrol -.7525
.5482
9 .203 -1.9928 .4878
71
Kontrol Vitamin E
tokotrienol .3350
.5482
9 .556 -.9053 1.5753
Vitamin E
tokotrienol +
Asam
ascorbat
.7525 .5482
9 .203 -.4878 1.9928
Based on observed means.
The error term is Mean
Square(Error) = .601.
Homogeneous Subsets
Eosinofil
Perlakuan N
Subset
1
Duncana Vitamin E tokotrienol +
Asam ascorbat 4 2.5000
Vitamin E tokotrienol 4 2.9175
Kontrol 4 3.2525
Sig. .222
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = .601.
a. Uses Harmonic Mean Sample Size = 4.000.
72
BASOFIL
Tests of Normality
Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statisti
c df Sig.
Statisti
c df Sig.
Basofi
l
Vitamin E
tokotrienol .282 4 . .862 4 .268
Vitamin E
tokotrienol + Asam
ascorbat
.326 4 . .899 4 .424
Kontrol .297 4 . .851 4 .229
a. Lilliefors Significance Correction
Uji RAL menggunakan Uji OneWay Anova
Between-Subjects Factors
Value Label N
Perlakuan 1 Vitamin E
tokotrienol 4
2 Vitamin E
tokotrienol
+ Asam
ascorbat
4
3 Kontrol 4
73
Descriptive Statistics
Dependent Variable:Basofil
Perlakuan Mean
Std.
Deviation N
Vitamin E tokotrienol 1.2075 .15756 4
Vitamin E tokotrienol +
Asam ascorbat 1.2900 .20928 4
Kontrol .9150 .49890 4
Total 1.1375 .33888 12
Levene's Test of Equality of Error
Variancesa
Dependent Variable:Basofil
F df1 df2 Sig.
7.978 2 9 .010
Tests the null hypothesis that the error
variance of the dependent variable is
equal across groups.
a. Design: Perlakuan
74
Tests of Between-Subjects Effects
Dependent
Variable:Basofil
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 15.838a 3 5.279 49.878 .000
Perlakuan 15.838 3 5.279 49.878 .000
Error .953 9 .106
Total 16.790 12
a. R Squared = .943 (Adjusted R Squared = .924)
Estimated Marginal Means
Perlakuan
Dependent Variable:Basofil
Perlakuan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
Vitamin E tokotrienol 1.208 .163 .840 1.575
Vitamin E tokotrienol +
Asam ascorbat 1.290 .163 .922 1.658
Kontrol .915 .163 .547 1.283
75
Post Hoc Tests
Multiple Comparisons
Basofil
Games-Howell
(I) Perlakuan (J) Perlakuan
Mean
Differe
nce (I-
J)
Std.
Error Sig.
95% Confidence
Interval
Lower
Bound
Upper
Bound
Vitamin E
tokotrienol
Vitamin E
tokotrienol +
Asam ascorbat
-.0825 .13098 .81
0 -.4934 .3284
Kontrol .2925 .26159
.55
9 -.6907 1.2757
Vitamin E
tokotrienol +
Asam ascorbat
Vitamin E
tokotrienol .0825 .13098
.81
0 -.3284 .4934
Kontrol .3750 .27051
.42
8 -.5864 1.3364
Kontrol Vitamin E
tokotrienol -.2925 .26159
.55
9 -1.2757 .6907
Vitamin E
tokotrienol +
Asam ascorbat
-.3750 .27051 .42
8 -1.3364 .5864
Based on observed means.
The error term is Mean
Square(Error) = .106.
76
LIMFOSIT
Tests of Normality
Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statist
ic df Sig.
Statist
ic df Sig.
Limfo
sit
Vitamin E
tokotrienol .351 4 . .814 4 .129
Vitamin E
tokotrienol +
Asam ascorbat
.378 4 . .765 4 .053
Kontrol .384 4 . .775 4 .064
a. Lilliefors Significance
Correction
Uji RAL menggunakan Uji OneWay Anova
Between-Subjects Factors
Value Label N
Perlakuan 1 Vitamin E
tokotrienol 4
2 Vitamin E
tokotrienol
+ Asam
ascorbat
4
3 Kontrol 4
77
Descriptive Statistics
Dependent Variable:Limfosit
Perlakuan Mean
Std.
Deviation N
Vitamin E tokotrienol 38.7500 11.97728 4
Vitamin E tokotrienol +
Asam ascorbat 36.3750 13.45010 4
Kontrol 49.8325 4.39069 4
Total 41.6525 11.45613 12
Levene's Test of Equality of Error
Variancesa
Dependent Variable:Limfosit
F df1 df2 Sig.
1.650 2 9 .245
Tests the null hypothesis that the error
variance of the dependent variable is
equal across groups.
a. Design: Perlakuan
Tests of Between-Subjects Effects
Dependent Variable:Limfosit
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 21231.925a 3 7077.308 61.786 .000
Perlakuan 21231.925 3 7077.308 61.786 .000
Error 1030.915 9 114.546
Total 22262.840 12
a. R Squared = .954 (Adjusted R Squared = .938)
78
Estimated Marginal Means
Perlakuan
Dependent Variable:Limfosit
Perlakuan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
Vitamin E tokotrienol 38.750 5.351 26.644 50.856
Vitamin E tokotrienol +
Asam ascorbat 36.375 5.351 24.269 48.481
Kontrol 49.832 5.351 37.727 61.938
Post Hoc Tests
Multiple Comparisons
Dependent
Variable:Limfosit
(I) Perlakuan (J) Perlakuan
Mean
Differe
nce (I-
J)
Std.
Error Sig.
95% Confidence
Interval
Lower
Bound
Upper
Bound
LSD Vitamin E
tokotrienol
Vitamin E
tokotrienol +
Asam
ascorbat
2.3750 7.5679
0 .761 -14.7448 19.4948
Kontrol -
11.082
5
7.5679
0 .177 -28.2023 6.0373
Vitamin E
tokotrienol +
Asam
ascorbat
Vitamin E
tokotrienol
-
2.3750
7.5679
0 .761 -19.4948 14.7448
Kontrol -
13.457
5
7.5679
0 .109 -30.5773 3.6623
79
Kontrol Vitamin E
tokotrienol
11.082
5
7.5679
0 .177 -6.0373 28.2023
Vitamin E
tokotrienol +
Asam
ascorbat
13.457
5
7.5679
0 .109 -3.6623 30.5773
Based on observed means.
The error term is Mean
Square(Error) = 114.546.
Homogeneous Subsets
Limfosit
Perlakuan N
Subset
1
Duncana Vitamin E tokotrienol +
Asam ascorbat 4 36.3750
Vitamin E tokotrienol 4 38.7500
Kontrol 4 49.8325
Sig. .123
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 114.546.
a. Uses Harmonic Mean Sample Size = 4.000.
80
MONOSIT
Tests of Normality
Perlakuan
Kolmogorov-Smirnova Shapiro-Wilk
Statisti
c df Sig.
Statisti
c df Sig.
Monosi
t
Vitamin E
tokotrienol .173 4 . .981 4 .907
Vitamin E
tokotrienol + Asam
ascorbat
.253 4 . .908 4 .471
Kontrol .304 4 . .790 4 .085
a. Lilliefors Significance Correction
Uji RAL menggunakan Uji OneWay Anova
Between-Subjects Factors
Value Label N
Perlakuan 1 Vitamin E
tokotrienol 4
2 Vitamin E
tokotrienol
+ Asam
ascorbat
4
3 Kontrol 4
81
Descriptive Statistics
Dependent Variable:Monosit
Perlakuan Mean
Std.
Deviation N
Vitamin E tokotrienol 8.9175 2.13225 4
Vitamin E tokotrienol +
Asam ascorbat 9.2525 1.83409 4
Kontrol 11.5850 .62952 4
Total 9.9183 1.94962 12
Levene's Test of Equality of Error
Variancesa
Dependent Variable:Monosit
F df1 df2 Sig.
2.204 2 9 .166
Tests the null hypothesis that the error
variance of the dependent variable is
equal across groups.
a. Design: Perlakuan
Tests of Between-Subjects Effects
Dependent Variable:Monosit
Source
Type III Sum
of Squares df Mean Square F Sig.
Model 1197.371a 3 399.124 144.146 .000
Perlakuan 1197.371 3 399.124 144.146 .000
Error 24.920 9 2.769
Total 1222.291 12
a. R Squared = .980 (Adjusted R Squared = .973)
82
Estimated Marginal Means
Perlakuan
Dependent Variable:Monosit
Perlakuan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
Vitamin E tokotrienol 8.918 .832 7.035 10.800
Vitamin E tokotrienol +
Asam ascorbat 9.253 .832 7.370 11.135
Kontrol 11.585 .832 9.703 13.467
Post Hoc Tests
Multiple Comparisons
Dependent
Variable:Monosit
(I) Perlakuan (J) Perlakuan
Mean
Differe
nce (I-
J)
Std.
Error Sig.
95% Confidence
Interval
Lower
Bound
Upper
Bound
LSD Vitamin E
tokotrienol
Vitamin E
tokotrienol +
Asam
ascorbat
-.3350 1.1766
3
.78
2 -2.9967 2.3267
Kontrol -
2.6675
*
1.1766
3
.05
0 -5.3292 -.0058
Vitamin E
tokotrienol +
Asam
ascorbat
Vitamin E
tokotrienol .3350
1.1766
3
.78
2 -2.3267 2.9967
Kontrol -
2.3325
1.1766
3
.07
9 -4.9942 .3292
83
Kontrol Vitamin E
tokotrienol
2.6675
*
1.1766
3
.05
0 .0058 5.3292
Vitamin E
tokotrienol +
Asam
ascorbat
2.3325 1.1766
3
.07
9 -.3292 4.9942
Based on observed means.
The error term is Mean
Square(Error) = 2.769.
*. The mean difference is
significant at the .05 level.
Homogeneous Subsets
Monosit
Perlakuan N
Subset
1
Duncana Vitamin E tokotrienol 4 8.9175
Vitamin E tokotrienol +
Asam ascorbat 4 9.2525
Kontrol 4 11.5850
Sig. .058
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 2.769.
a. Uses Harmonic Mean Sample Size = 4.000.