IDENTIFICATION OFIDENTIFICATION OFENTEROBACTERIACEAEENTEROBACTERIACEAE

Prepared byPrepared by::Abdullah AliAbdullah Ali

Supervised by:Dr.Abdellraof el-ManamaDr.Nedal Fayyad

بغزه االسالمية الجامعةالعلوم كلية

الطبية التحاليل قسم

Islamic University of GazaFaculty of scienceMedical technology department

Characteristics of EnterobacteriaceaeMembers of the Enterobacteriaceae are Gram-negative, straight rods, some of which are motile. Most species grow well at 37°C,

although some species grow better at 25 - 30°C. They are facultativelyanaerobic, oxidase-negative and catalase-positive (except Shigella dysenteriae type 1). They are distributed worldwide and may be

found in soil, water, plants and animals.

Common genera of the family Enterobacteriaceae

1) Citrobacter species2 )Enterobacter species

3 )Escherichia species4 )Hafnia alvei

5 )Klebsiella species6 )Shigella species

7) Morganella morganii

8 )Proteus species9 )Providencia

species10 )Salmonella

species11 )Serratia species12 )Yersinia species

Incidence of Enterobacteriaceae Associated with Bacteremia

EscherichiaEscherichia

Escherichia coliEscherichia coli

SalmonellaSalmonella

EnterobacterEnterobacter

Enterobacter aurogeouns Enterobacter sakazakii

ShigellaShigella

CitrobacterCitrobacter

Can they ferment lactose?

Can they use citric acid as their sole carbon source?

Can they use citric acid as their sole carbon source?

Do they produce acetoin?

EscherichiaSalmonellaShigella

(Yes)

(Yes)

(Yes)

(Yes)

(No)

(No)(No)

(No)

Citrobacter Enterobacter

Can they ferment lactose?

Can they use citric acid as their sole carbon source?

Tests:

From the previous slide we observe that we need to do the following tests:

1)Lactose fermentation

2)Citrate test

3)Voges-proskauer test

Citratetest

Some disease causing by Some disease causing by Enterobacteriaceae speciesEnterobacteriaceae species

speciesdisease

E. coliE. coli.diarrhea, invasive disease

SalmonellaSalmonella•diarrhea, septicemia, pneumonia

ShigellaShigella .dysentery

ConclusionFamily Enterobacteriaceae have the followingcharacteristic:

1)Gram-negative rod2)Non-sporeforming bacteria

3)Facultative anaerobe4)Motile by peritritchous flagella

5)Oxidase negative6)Non acid fast bacteria

7)Produce nitrate reductase

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