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SCI论文写作

陈如平 2013.8.13

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Title

Abstract

Introduction

M&M

Results

Discussion

References

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Materials and Methods

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1. Give the species of the organism, or the cell lines you are studying.

-mice：Twenty 12-week old female BALB/c mice were purchased from Jackson Laboratories. Mice were raised under standard conditions of…and fed on …with access to free access to water.

品系，数目，性别，体重，周龄，来源及饲养条件； (approved by the Institutional Animal Care and Use Committee of…).

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HCT116 cells (a gift of B. Vogelstein) were grown in McCoy's medium plus 10% FBS, P/S. All cells were maintained at 37°C in 5% CO2.

- Cell line: 来源，培养基及培养条件

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2. Describe the source of chemicals and reagents.

-from a company, give the name and location of the company.

-gifted by some person, give the name, institute and location.

平时整理一份常用试剂的公司

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3. Give details such as temperature, pH, amount of reagent and sampling times.

4. Use a method that is common in your field, you can reference the paper that describes this method.

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5. Use scientific and specific language. Don’t use vague or imprecise words

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实验方法的描述就是一系列专业科学动词的运用

cultivate; culture; decant; destain; determine; develop; dialyze; digest; disperse; dissolve;divide; elute; express; extract; filter; freeze;fuse; grow; hybridize; immobilize; immunoprecipitate; incorporate; incubate; inject; inoculate; resuspend; separate; stain; transfer; transfect and etc.

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常用方法总结

Western blot; qRT-PCR; MTT; BrdU; Tunel staining; ELISA; Immunocytochemistry; Immunohistochemistry; Scan flow cytometer; RNAi; Overexpression etc.

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Results

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结果的表现形式

Table Graph Figure

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To tell a story: in step by step style

Initial hypothesis

Experiments to test

Supported or not

Second question/hypothesis

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首先整理出一套清晰的思路，按照思路来作图。

写一个以 subheading为主的框架，然后在具体写作。

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To/in order to find/determine/discover/ measure/study/analyze/test/better understand …

Begin with

-To determine whether the defect affects transcription of the gene, we isolated RNA from the cells and quantified the RNA by RT-PCR.

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Results description

The results/data showed/exhibited/demonstrated/…, or we found/… that….reduced/increased/decreased/up or down-regulated….(Fig or Table).

-qualitative description and quantitative description

-significant statistics （ variable over time ；comparison among groups ； relationship between variables ）

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-To explain all the symbols and different groups shown in the figure.

-Brief description of the experiment.

Figure legend

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Discussion

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Significance

Explain what is new in your work and why this matters to research focus.

-To explain the importance of the discoveries

-To spread the implications of the study

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Begin with

-The main findings of this study… /In the present study, we demonstrate/describe/find/…

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-Whether get the expected results or not ， and explain.

-Compare with other studies: in line with or not, and give the possible reason.

-Present an idea and list related studies to support.

根据结果内容，总结 3-4个点，分别进行讨论。

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End with

-limitation

-implication

-need for more research

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Abstract

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1. Background

2. Purpose

3. Methods

4. Results

5. Conclusions

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Background -Briefly describe the background and context to the study.

Purpose -Identify the particular research problem, objectives or hypotheses.-Sometimes, point to a need for research/more research.

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Methods-what you did or measured, and how you did it.

Results -List important data specifically and briefly.

Conclusions-State the contribution of the work to the area of research and its implications.

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熟读唐诗三百首不会写诗也会吟

读破万卷书 , 下笔如有神

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Thanks for your attention!