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Introduction of Arbutin
1. Overview
Arbutin is commercially used as a skin-whitening agent in cosmetic industry. It works by competitive
inhibition of the enzyme, tyrosinase, a key enzyme in the synthesis of melanin.
Depending on the spatial structure of the glycosidic linkage between glucose and hydroquinone, arbutin
forms two epimers: α-arbutin and β-arbutin.
Product name CAS Number Molecular formula Molecular structure
Beta-Arbutin 497-76-7 C12H16O7
Alpha-Arbutin 84380-01-8 C12H16O7
Tel +86-571-28521283 Fax +86-571-28521284
Hangzhou Mobel Biotechnology Co.,ltdAddress: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China
Hompage: www.mobelchem.com Email:[email protected]
2. Natural source and Industrial Process Method
Beta-Arbutin
Beta-Arbutin is often referred to as just Arbutin. It is can be naturally extracted from plants such as
bearberry and synthesized by chemical method. Synthesized Beta-Arbutin is currently one of the most
widely used hypopigmenting agents in cosmetics, while plant-derived drugs containing Beta-Arbutin
were initially used for the treatment of urinary tract infections, cystitis, kidney stones, and as diuretics in
the medical field.
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
Beta-Arbutin Flow chart
Alpha-Arbutin
Alpha-Arbutin is enzymatically synthesized from hydroquinone and saccharides through
transglycosylation reaction. Various carbohydrate-active enzymes such as α-amylase, α-glucosidase,
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
transglucosidase, sucrose phosphorylase, and dextransucrase have been employed to synthesize Alpha-
Arbutin. It is one of the most advanced skin lightening ingredients on the market.
Alpha-Arbutin Flow chart
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
3. Specification
Product name Specification
Beta-Arbutin 99.5% HPLC
Alpha-Arbutin 99.5% HPLC
Beta-Arbutin Specification
ANALYSIS SPECIFICATION
Assay ≥99.5%
Appearance White crystalline powder
Melting Point 199~201±0.5℃
PH(1%water solution) 5.0-7.0
Specific Optical Rotation -66±2°
Loss on drying ≤0.5%
Residue on ignition ≤0.5%
Hydrochinone ≤10ppm
Heavy metals ≤20ppm
Arsenic ≤2ppm
Total Plate Count ≤300cfu/g
Total Yeast Count ≤100cfu/g
Alpha-Arbutin Specification
ANALYSIS SPECIFICATION
Assay ≥99.5% HPLC
Appearance White crystalline powder
Hydroquinone Negative
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
Melting Point 203-206(±1)℃
Specific Optical Rotation [a]20
D= + 174.0°- +186.0°
Infrared peak value 1514cm-1
; 1229 cm-1
; 1215 cm-1
; 1059 cm-1
; 1034 cm-1
Solubility Soluble in water, slightly soluble in ethanol
Clarity Solution should be clarify, none suspended matters
PH(1% water solution) 5.0-7.0
Loss on drying ≤0.5%
Residue on Ignition ≤0.5%
Heavy metals ≤10ppm
Lead ≤2ppm
Arsenic ≤2ppm
Mercury ≤1ppm
Total Plate Count ≤100cfu/g
Total Yeast & Mold ≤50cfu/g
E.Coli Negative
Salmonella Negative
Staphylococcus Negative
4. Arbutin Working Mechanism
Skin whitening
Arbutin exhibits potent melanin-inhibiting properties and confers whitening effects on the skin. Sun
damage and injury or inflammation to the skin from acne, eczema or psoriasis are the two main factors
that cause melanin production on the skin. Arbutin can reduce the accumulation of pigment by inhibiting
the production of melanin, which accumulates on the skin when tyrosine is oxidized. An enzyme called
tyrosinase is the catalytic for this reaction. Scientists found arbutin has shown inhibitory actions against
tyrosinase. When topically apply skincare products containing Arbutin, the inhabitation of tyrosinase
results in the decrease of melanin, thus, a whiter complexion.
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
Hyperpigmentation in the epidermis is caused by excessive melanin synthesis due to UV irradiation,
wound or stress. Tyrosinase is one of the key enzymes involved in melanin synthesis. The enzyme
catalyses the first two steps in melanin synthesis: the hydroxylation of tyrosine to DOPA and the
oxidation of DOPA to dopaquinone. Arbutin has an direct inhibitory function against tyrosinase, which
leads to the prevention of melanin formation and a whitening effect on the skin.
5. Alpha-Arbutin V.S. Beta-Arbutin
Recent research showed that Alpha-Arbutin has a 10-fold stronger inhibitory effect on the activity of
tyrosinase from human malignant melanoma cells than that of Beta-Arbutin. The α-glucosidic bond found
in Alpha-Arbutin offers higher stability than the β form found in the related Beta-Arbutin. Moreover,
Alpha-Arbutin hardly inhibited cell growth at 1 mmol/L, while Beta-Arbutin inhibit it significantly at the
same concentration. It is, therefore, considered that Alpha-Arbutin is an effective and safe ingredient for
cosmetics. Alpha arbutin is very expensive. That is the one downside.
Differences between Alpha-Arbutin V.S. Beta-Arbutin
Production Tyrosinase
inhibitory efficacy Stability Safety Price
Alpha-
Arbutin
enzymatically
synthesized 10 times stronger higher safer
More
expensive
Beta-Arbutin
Extracted; or
chemically
synthesized
weaker lower inhibit cell
growth cheaper
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
Alpha-Arbutin Water Solubility
6. Feature of PLAMED Arbutin
1. Beta-Arbutin is produced by environment friendly new tech, annual productivity 100mts, table supply.
2. Loss on drying strictly controlled, less than 0.5%
3. Hydroquinone residue less than 10ppm, Hydroquinone is poisonous and is forbidden in cosmetics.
The residue control is very important.
7. Arbutin Application and Reference amount
Arbutin is used as a skin whitening agent, a sunscreen agent and an antioxidant in cosmetic formulations.
Arbutin soap, cream, serum and lotion are among the major lightening products you will found in the
market.
Product name Reference amount
Beta-Arbutin 1-7%
Alpha-Arbutin 0.2-5%
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
Arbutin’s advantage over traditional skin-whitening products:
Arbutin can inhibit melanogenesis without causing melanocyte toxicity, unlike traditional depigmenting
agents such as hydroquinone, corticosteroids, and kojic acid.
8. Note
1) Arbutin breaks down easily in acidic conditions, so the pH value of cosmetics containing Arbutin
should be greater than 6.0.
2) Please dissolve arbutin in 50℃ hot water before adding it to skin whitening products. (Arbutin is
soluble in hot water, methanol or alcohol. Arbutin is partially soluble in cold water. Arbutin is
insoluble in aether, chloroform or benzene.)
3) Antioxidants should be added to cosmetics containing Arbutin.
9. Storage
Please tightly sealed Arbutin in containers. Store it in a cool dry place and away from sunlight and
oxidizing agents.
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd
Packed in 25kg paper drum, 1kg aluminum foil package inside.
Address: 25 No.Bio-chemicals Technical Zoon,Yuhang District, Hangzhou city,Zhejiang P.R China 310001Homepage: www.mobelbio.com Email: [email protected]
Tel +86-571-28021283 Fax +86-571-28093522
Hangzhou Mobel Biotechnology Co.,ltd