MagSi Beads - AMSBIO · optical, surface and binding properties. MagSi beads are specifically designed for separation, concentration and ... Magnetic bead-based kit for PCR clean-up,

MagSi Beads Magnetic Silica Beads for Research in Life Science and In vitro Diagnostics

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Contents Page

Introduction: AMSBIO MagSi Magnetic Beads……….……….……….……………………

MagSi STA……………....……….….……….……….……….……….……….……….………..…

MagSi products for Genomic applications……………………….……….……….………..

MagSi-gDNA Blood Kit……….……….……….………….……….……….………….………...

MagSi DNA Clean Kit………………………………….……….……….……….………….…....

MagSi-NGSPREP Size Selection…………………………….……….……….……………………

MagSi-Proteomics……….……….……….……….……….……….……….……….……….....

MagSi-WCX and MagSi-WAX……….……….……….………….……….……….…………....

MagSi-protein A and G……….……….……….……….……….……….……….………........

MagSi-Tools……….……….……….……….……….……….……….……….……….……….….

MagCustom……….………….………….………….………….………….………….….............

Separation Tools…….………….………….………….………….………….……………………

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In partnership with MagnaMedics

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MagSi Magnetic Beads

AMSBIO supplies a unique range of magnetic and non-magnetic silica beads for biological applications. Our MagSi

brand magnetic beads range in size from 150nm to 5µm. They can have a wide range of surface properties,

functional groups, and magnetic properties.

Our fast and flexible production process facilitates unrestricted customization possibilities with regard to sizes, kinetics,

optical, surface and binding properties. MagSi beads are specifically designed for separation, concentration and

purification of bio-molecules and specific cells or cell compartments.

Figure 1: Encapsulation and surface possibilities for MagSi beads

MagSi Products

MagSi magnetic silica beads can be coated with

streptavidin, protein A, protein G, carboxyl groups,

or various other chemical groups, proteins, or

oligonucleotides. This enables the specific capture

of desired target molecules, cell types, or

organelles from large or small volumes of solution.

Our beads can also incorporate fluorescent dyes or

quantum dots. This allows for both manual and

imaging and quantitative detection in automated

systems. The MagSi-Tools are ideal for your own

experiments and coupling procedures.

MagSi magnetic beads are designed to meet the

demanding needs of biological applications. We

know that scientists need state of the art,

innovative products in order to remain at the

forefront of their fields of interest.

High customization potential (e.g. sizes, surfaces,

colours)

Speed and robustness

Flexibility and scalability

Ideal for automated systems

High binding capacity

nm & µm sized magnetic beads

Proven for downstream applications like PCR etc.

Minimal non-specific absorption

MagSi ADVANTAGES:

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Customer Development

Tailored Products

Silica,

COOH,

NH2

SH

CHO

MagSi-Tools

Streptavidin

covalently

bound to silica

Ferro fluid (30-50%)

Fluorescent dye

Regular Dye

Isolation of biotinylated target

molecules

Conjugation to biotin-tagged

antibodies for protein capture or

immunoassay experiments

Cell/bacteria isolation MagSi-STA

Antibody purification

Conjugation to IgG

antibodies for protein

capture or immunoassay

experiments

Protein A or G

covalently

bound to silica


Fluorescent dye

Regular Dye

MagSi-protein A

MagSi-protein G

Protein Purification,

Concentration and/or

desalting of peptide and

protein digests

C4, C8,or C18

Hydrophobic

groups


Fluorescent dye

Regular Dye

MagSi-Proteomics


Fluorescent dye

Regular Dye

Isolation of genomic DNA,

PCR clean up,

DNA Sanger clean up

NGS size selection

Plain silica

COOH

MagSi beads for

DNA isolation

Sample preparation, Pre-

fractionation prior to mass

spectrometry, SDS-PAGE analysis,

Protein and peptide analysis,

Serum/plasma profiling

Ferro fluid

(30-50%)

Fluorescent dye

Regular Dye

MagSi –WAX/WCX

Anion exchangers

Cation exchangers


Fluorescent dye

Regular Dye

Summary of MagSi Magnetic Beads

Applications

Surface Modification Options

Magnetic Properties and Optical Options

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MagSi STA

Magnetic silica particles with high quality streptavidin covalently attached to the

bead surface. Applications include immunoassays using biotin-tagged antibodies

and capture or purification of biotinylated molecules. Various types of this product

are available, with different size, streptavidin coating chemistry (“Type”), and

binding capacity. All parameters can be customized on request.

Cat # Description Conc. Size* Amount Binding capacity (pmol

biotin/mg)

Type*

MD16001 MagSi-STA 600 10mg/ml 600nm 2ml 3500-5000 C



MD21001 MagSi-STA 600 BI 10mg/ml 600nm 2ml 6000-6800 C



MD01001 MagSi-STA 1.0 10mg/ml 1μm 2ml 3500-5000 C



MD06001 MagSi-STA 1.0 L 10mg/ml 1μm 2ml 1200-2000 C



MD25001 MagSi-STA 1.0 TL 10mg/ml 1μm 2ml 1200-2000 T



MD29001 MagSi-STA 1.0 TS 10mg/ml 1μm 2ml 3500-5000 T



MD33001 MagSi-STA 3.0 L 10mg/ml 3 μm 2ml 700-1200 T



MD37001 MagSi-STA 3.0 TL 10mg/ml 3 μm 2ml 500-900 T



* Type refers to the applied streptavidin coupling chemistry. C (Carboxyl): This type is intended for applications which require a relatively hydrophilic

surface and also includes a spacer. T (Tosyl): This type is intended for applications which require beads which are more hydrophobic.

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MagSi-IVD Trial kit

This kit includes 1 ml of the 8 different MagSi-STA products listed above and is intended for evaluation purposes during

the trial phase of developing new assays, or bead replacement in existing assays.

Cat # Description Conc. Size* Amount

MD50001 MagSi-IVD Trial kit 10mg/ml 600nm, 1μm, 3.0 μm 8 x 1ml

MagSIGNAL-STA 300

MagSIGNAL beads are intended for use in magnetic immunoassays (MIAs). These assays use paramagnetic beads as

detection labels instead of conventional enzymes, fluorophores, or luminescent molecules. The presence of

paramagnetic beads is detected by a magnetometer, and the signal thus measured is proportional to the

concentration of the analyte. MagSIGNAL beads are superparamagnetic beads with a mean diameter of 300 nm

which are ideal for magnetic immunoassays in a variety of formats including as conventional lateral flow tests,

microfluidic applications and biochips.


MD01101 MagSIGNAL-STA 300 10mg/ml 300nm 2ml



MagSIGNAL-STA 300 beads have high quality streptavidin covalently attached to the

surface. They are intended for use with biotinylated antibodies as a detection label in

magnetic immunoassays, or as solid support phase in immunoassays with other readout

techniques.

MagSIGNAL-COOH 300

MagSIGNAL-STA 300 beads have a carboxy-modified surface, intended for immobilization of

antibodies or proteins with carbodiimide coupling chemistry with NH2 -containing molecules.

These particles can be used as detection label in magnetic immunoassays or as solid support

phase in immunoassays with other readout techniques.





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MagSi-DNA

Ultra-fast magnetic silica particles with instant magnetic separation and short suspension time. Intended for nucleic

acid isolation from various sources (blood, cells, bacteria etc.) for manual and automated work-flow.

MagSi products for Genomic applications

Magnetic beads can be used as solid support phase in DNA extraction and purification protocols by a

simple bind/wash/elute principle. The products below are intended for own development of protocols

and are suitable for various sample sources and buffer systems. MagSi-beads for genomic applications

are available with a range of physical properties and a silica or carboxyl modified surface.


MD01017 MagSi-DNA 300mg/ml 300nm 2ml




MD01018 MagSi-DNA allround 20mg/ml 1.2µm 2ml



MagSi-DNA allround

Ultra-fast magnetic silica beads with optimized magnetic content for fast separation and long suspension time without

mixing. Intended for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for manual and automated

work-flow.

MagSi-DNA COOH

Magnetic silica particles with instant magnetic separation and short suspension time. Intended for nucleic acid

isolation from various sources (blood, cells, bacteria etc.) for manual and automated work-flow. Under specific

conditions, the carboxylated surface enables higher yield and purity from samples.

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MD01020 MagSi-DNA allround COOH 20mg/ml 1.2µm 2ml



MagSi-DNA allround COOH

Magnetic silica beads with optimized magnetic content for fast separation and long suspension time without mixing.

Intended for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for manual and automated

workflow. Under specific conditions, the carboxylated surface enables higher yield and purity from samples.

MagSi-DNA & MagSi-DNA COOH MagSi-DNA allround & MagSi-DNA allround COOH

Mean Size 300 nm 1.2 μm

Concentration 300 mg/ml 20 mg/ml

Particle count/ml 4,6 * 1012 1,7 * 1010

DNA binding capacity/

isolation (20 μl bead solution)

up to 8 μg up to 6 μg

DNA yield/mg bead up to 1,3 μg/mg up to 3.2 μg/mg

Stability pH 3-11 pH 3-11

Specifications of the MagSi Beads for DNA extraction.


MD01019 MagSi-DNA COOH 300mg/ml 300nm 2ml



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MagSi-gDNA Blood Kit for isolation of gDNA from human and mammalian whole blood or buffy coat, non mammalian

blood samples, cerebrospinal fluids, bone marrow and swabs .

Cat # Description Voume

MD60001 MagSi-gDNA Blood Kit 1,000 isolations

MD60002 MagSi-gDNA Blood Kit 10,000 isolations

MagSi DNA CleanFIX Kit

Magnetic bead-based kit for PCR clean-up, removal of DyeDeoxy™ terminators from DNA cycle sequencing reactions,

and purification and concentration of DNA fragments from enzymatic reactions like cDNA synthesis or enzyme

restriction digestions. MagSi-DNA cleanFIX offers one single product for efficient PCR clean-up and Dye terminator

removal from DNA Sanger sequencing reactions, enabling researchers to address various genomic clean-up steps with

the same product. MagSi-DNA cleanFIX uses flexible protocols and is easy to automate for high-throughput processing.

Cat # Description Volume

MD60013 MagSi-DNA CleanFIX 400 / 800 purifications



MagSi gDNA Blood Kit

Figure 2: Gel electrophoresis of PCR samples. Lanes A: unpurified PCR sample showing primer dimers, lanes B:

samples purified with MagSi-DNA cleanFIX, lanes C: sample purified with AMPure® XP; lanes M: DNA ladder.

Figure 3: Sequence electropherogram after purification of pGEM Plasmid Template DNA with MagSi-DNA

CleanFIX

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MagSi-NGSPREP

MagSi-NGSPREP is designed for an optimized purification of next generation libraries preparation through efficient clean

up of the successive enzymatic reactions (ie. End repair, dA-Tailing, Adaptor ligation). MagSi-NGSPREP is used for clean-

up of libraries targeting sequencers such as Life Technologies (ABI SOLiD, Ion Torrent); Illumina’s (HiSeq 2000, MiSeq,

GAIIx); and Roche’s 454). Magnetic bead based MagSi-NGSPREP offers an efficient solution for clean-up and size

selection in library preparation of NGS sequencing reactions. The simple and flexible protocol can be adjusted to your

specific application and NGS platform.

Cat # Description Volume

MD60021 MagSi-NGSPREP 5 mL



High quality results

• High recovery of DNA fragments, more than 70%

• Excellent removal of enzymes, primers, oligonucleotides, polymerase and other contaminants

• Fragment size selection adjustable between 100 and 1000 base pairs

• Guarantees consistent sequencing results

Simplify your routine

• One product for all clean-up and size selection steps in the library preparation workflow

• Simple bind-wash-elute procedure

• Protocol easily adjustable for clean-up or size selection using specific reagent-to-sample ratios

• Manual and automated use

• Meets the requirements of common library preparation kits

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MagSi-Proteomics beads Zip tips

Product types available C4, C8, C18, WCX, WAX C4, C18, SCX

Binding capacity >10µg/isolation 5µg/isolation

Samples volume scalability Yes No

Robotic work flow usage Yes, no back-pressure Limited, back-pressure

Price comparison of product 2 x Less expensive as ZipTips®

MagSi Proteomics

Magnetic silica particles with C4, C8 or C18 modified surface for sample preparation prior to mass

spectrometry analysis. The relatively low hydrophobicity of MagSi-proteomics C4 allows the

purification and fractionation of larger biomolecules like proteins. MagSi-proteomics C8 have an

intermediate hydrophobicity and are suitable for sample preparation in for proteomic profiling

and biomarker research. MagSi-proteomics C18 are ideal for the purification, concentration and

desalting of peptides and protein digests.

desalting of proteins, peptides and proteolytical digests prior to mass spectrometry

concentration of low concentrated proteins or peptides

fractionation of a complex peptide or protein mixture

Use of magnetic beads is an economical, safe and easy-to-use alternative to commonly used pipette or column

based methods.

Figure 4: Schematic representation of peptide/protein cleanup using MagSi-DNA beads

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MagSi-Proteomics C4 Magsi-Proteomics

C8

MagSi-Proteomics

C18

MagSi-WCX MagSi-WAX

Peptide profiling serum - ++ + + +

Peptide profiling urine - ++ + + +

Peptide profiling saliva - ++ + + +

Biomarker profiling from tissues - - - ++ ++


MD01014 MagSi-proteomics C4 10mg/ml 1.2µm 2ml









MagSi-WAX and MagSi-WCX

MagSi-WAX (weak anion exchange) and MagSi-WCX (weak cation exchange) beads are an ideal tool for the

reduction of protein or peptide complexity (e.g. cell lysates).

Main applications include:

• Sample preparation and pre-fractionation prior to mass spectrometry (e.g. MALDI-TOF analysis)

• SDS-PAGE analysis

• Protein and peptide separation for multiple downstream applications

• Biomarker analysis and serum/plasma profiling

The high magnetic strength makes them applicable for both manual and automated/robotic fractionation, because

the beads will typically collect in less than 1 minute when magnetic force is applied. This quick and complete

separation gives very good reproducibility since no beads will be lost during washing steps. The quick protein

adsorption, desorption and magnetic collection significantly shortens the protocol time over conventional column

based ion exchange chromatography.

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MagSi Protein A and G

Protein A and Protein G bind to Fc regions of immunoglobulins. After binding onto a

magnetic beads with a coating Protein A or Protein G, immobilized immunoglobulins

can be used for immunoprecipitation of various biomolecules, or can be eluted in a

native or denatured state.


MD01025 MagSi-WAX 20mg/ml 1.2µm 2ml

MD02025 MagSi-WAX 20mg/ml 1.2µm 10ml

MagSi-WAX


MD01023 MagSi-WCX 20mg/ml 1.2µm 2ml

MD04023 MagSi-WCX 20mg/ml 1.2µm 10ml

MagSi-WCX


MD10011 MagSi-protein A 600 10mg/ml 600nm 1ml

MD11011 MagSi-protein A 600 10mg/ml 600nm 5ml

MD01011 MagSi-protein A 1.0 10mg/ml 1µm 1ml

MD02011 MagSi-protein A 1.0 10mg/ml 1µm 5ml


MD10012 MagSi-protein G 600 10mg/ml 600nm 1ml

MD11012 MagSi-protein G 600 10mg/ml 600nm 5ml

MD01012 MagSi-protein G 1.0 10mg/ml 1µm 1ml

MD02012 MagSi-protein G 1.0 10mg/ml 1µm 5ml

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MD16003 MagSi-S 600 10mg/ml 600nm 2ml



MD01003 MagSi-S 1.0 10mg/ml 1µm 2ml



MD41003 MagSi-S 3.0 10mg/ml 3μm 2ml



MagSi Tools

MagSi-Tools are surface activated magnetic beads for immobilization of proteins (e.g. antibodies,

enzymes), peptides, nucleic acids or other molecules of interest. Different surface modifications allow for

choosing the optimal product for the right molecule to be coupled, and for the intended application.

The MagSi-S platform has a broad range of functionalisation possibilities such as COOH, NH2, SH, CHO,

tosyl, hydrazide and epoxy. MagSi-Tools products are available with 600nm, 1.0 µm or 3.0 µm mean

diameter.


MD16004 MagSi-S-COOH 600 10mg/ml 600nm 2ml



MD01004 MagSi-S-COOH 1.0 10mg/ml 1µm 2ml



MD41004 MagSi-S COOH 3.0 10mg/ml 3μm 2ml



MagSi-S COOH

Superparamagnetic silica particles with a carboxyl modified surface. Intended for carbodiimide coupling chemistry with

NH2-containing molecules.

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MagSi-S NH2

Super paramagnetic silica particles with NH2 modified surface. Intended for carbodiimide coupling chemistry with

COOH-containing molecules or aldehyde coupling chemistry.


MD16005 MagSi-S NH2 600 10mg/ml 600nm 2ml



MD01005 MagSi-S NH2 1.0 10mg/ml 1µm 2ml






MagSi-S SH

Super paramagnetic silica particles with modified surface for SH coupling chemistry.


MD18006 MagSi-S SH 600 10mg/ml 600nm 10ml

MD19006 MagSi-S SH 600 10mg/ml 600nm 100ml

MD03006 MagSi-S SH 1.0 10mg/ml 1µm 10ml




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MD18006 MagSi-S CHO 600 10mg/ml 600nm 10ml

MD19007 MagSi-S CHO 600 10mg/ml 600nm 100ml

MD03007 MagSi-S CHO 1.0 10mg/ml 1µm 10ml




MagSi-S CHO

Super paramagnetic silica particles with aldehyde modified surface. Intended for aldehyde coupling chemistry with

NH2 containing molecules.

MagSi-S Tosyl

Super paramagnetic silica particles with tosyl modified surface. Intended for tosyl coupling chemistry with antibodies

and proteins.


MD16008 MagSi-S Tosyl 600 10mg/ml 600nm 2ml



MD01008 MagSi-S Tosyl 1.0 10mg/ml 1µm 2ml






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MagSi-S Hydrazide

Super paramagnetic silica particles with hydrazide modified surface. Intended for immobilization of antibodies and

glycoproteins using aldehyde coupling chemistry with specific orientation.

MagSi-S Epoxy

Super paramagnetic silica particles with epoxy modified surface. Intended for epoxy coupling chemistry to enzymes

and other NH2 containing molecules.


MD16013 MagSi-S Hydrazide 600 10mg/ml 600nm 2ml



MD01013 MagSi-S Hydrazide1.0 10mg/ml 1µm 2ml



MD41013 MagSi-S Hydrazide 3.0 10mg/ml 3µm 2ml




MD16010 MagSi-S Epoxy 600 10mg/ml 600nm 2ml



MD01010 MagSi-S Epoxy 1.0 10mg/ml 1µm 2ml






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Magnetic separation products

Cat # Description Description and volume

MD90001 MM-Separator M12 + 12 Magnetic separator for manual processing in 12 x 1.5 ml and 12 x 2

ml tubes.

MD90002 MM-Separator M96 Magnetic Separator for isolation of MagSi beads in microtiterplate

format (96 wells)

MD90003 MM-Separator PCR strip

adapter

Adapter module for MM-Separator M96, for manual processing in

PCR tube strips

MD90005 MM-Separator 96 SBS Magnetic separator for automated processing in 96-well PCR

microplates

MD90006 MM-Separator 384 SBS Magnetic separator for automated processing in 384-well PCR

microplates

MM-Separators are intended for manual processing in tubes, tube-strips and microplates. MM-Separators SBS are

intended for automated processing of magnetic beads and kits in 96 and 384 well microplates. The separators include

a SBS standard registration base for easy placement on liquid handling instruments, and are suitable for separation in

PCR microplates and many other microplates.

Cat # Description Phase

MD80001 MagCustom Feasibility Study Phase 1

MD80002 MagCustom Project Define Phase Phase 2

MD80003 MagCustom Production Phase Phase 3

MagCustom

Tailor made magnetic and non magnetic beads – rapid prototyping and standardization.

MagSi is a registered trademark of MagnaMedics

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www.amsbio.com

Our Contact Details: AMS Biotechnology (Europe) Ltd - UK & Rest of World 184 Park Drive, Milton Park Abingdon OX14 4SE, U.K. Tel: +44 (0) 1235 828 200 Fax: +44 (0) 1235 820 482 AMS Biotechnology (Europe) Ltd – Switzerland Centro Nord-Sud 2E CH-6934 Bioggio-Lugano. Tel: +41 (0) 91 604 55 22 Fax: +41 (0) 91 605 17 85 AMS Biotechnology (Europe) Ltd – Germany Bockenheimer Landstrasse 17/19 60325 Frankfurt am Main Tel: +49 (0) 69 779099 Fax: +49 (0) 69 13376880 amsbio LLC – United States 1035 Cambridge Street, Cambridge, MA 02141. Tel: +1 (617) 945-5033 Tel: +1 (800) 987-0985 Fax: +1 (617) 945-8218

Documents

MagSi Beads - AMSBIO · optical, surface and binding properties. MagSi beads are specifically designed for separation, concentration and ... Magnetic bead-based kit for PCR clean-up,