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Master of Science in Parasitology

اتـــــــليماجستري الطفي

Program Code: PAR600

Program Guide and

Logbook

2

C a n d i d a t e C u r r i c u l u m v i t a e

[Name]

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recent photo

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[telephone No]

[mobile No]

[mailing address]

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Experience

[Organization]

[your present job title]

[start date]

[location]

[responsibilities]

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[previous job title]

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[responsibilities]

3

Education

[certificates]

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[school or college]

Training

[any other training that will be useful in your job]

4

Filled by post graduate authorities

Date of Registration

First semester _______________________

Second semester _______________________

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5

TABLE OF CONTENTS:

Page

I – Welcome Statement 7

II - Mission Statement 7

III – Senior supervisor and affiliated departments and Hospitals 7

IV – Program specifications 7

A) Basic Information 7

B) Professional Information 8

1. Program Aim 8

2. Intended Learning outcomes 8

3. Academic standards 10

4. Curriculum structure and contents 10

5. Program courses: 11

6. Program admission requirements 12

7. Regulation for progression and program completion 12

8. Assessment Schedule and Weighing of Assessments 13

Course specification for Microbiology and Immunology 15

a - Basic information 15

b - Professional information 15

Course specification for Biochemistry 19

a - Basic information 19

b - Professional information 19

Course specification for Public Health 23

a - Basic information 23

b - Professional information 23

Course specification for Parasitology 26

a - Basic information 26

b - Professional information 26

Course specification for Molecular Biology 41

a - Basic information 41

b - Professional information 41

Course specification for Immunology 45

a - Basic information 45

b - Professional information 45

Course specification for Vector Biology 48

a - Basic information 48

b - Professional information 48

Course specification for Biostatistics 52

a - Basic information 52

b - Professional information 52

6

V - General information 55

VI – Your log book 55

1 - Introduction 55

2 - Supervisors 56

3 - Tables for training records 56

4 – Log book preview 73

VII - Head of department approval for the exam entry 73

Detachable forms:

VIII – Thesis follow up 74

IX - Evaluation forms 75

7

I. Welcome Statement:

The Department of Parasitology welcomes you to the Master of Science

in Parasitology. As a department we are committed to medical student education

and continuously strive to improve your educational experience.

This handbook presents information guide and logbook activity of the Master of

Science in Parasitology administered by the Parasitology department, Faculty of

Medicine, Ain Shams University.

II. Mission Statement:

The mission of the Faculty of Medicine, Ain Shams University is

“Preparation of a trained physician, researcher and life long tutor capable of

following standards of medical care and ethics, with managerial and technical

skills in his specialty. Furthermore, promotion outstanding programs of health

care to serve the society, environmental development and targeted scientific

research for continual improvement of health”.

The mission of this degree is” to prepare trained parasitologists, who have

detailed knowledge and understanding of the biology, life cycles, pathogenesis,

clinical manifestations, immune response, and basic and advanced diagnosis of

parasitic infections in humans and their relevance for human health. The

graduates of this program should also be able to work with other health authorities

to prevent and control the parasitic infection in the society”. The student should

Carry out and write up a research project (MSc. Thesis).

III. Senior Supervisor and Affiliated Departments and Hospitals

Senior Supervisor

Prof.

-------------------------------

-------------------------------

-------------------------------

E-mail:

Affiliated Departments and Hospitals

-------------------------------

-------------------------------

-------------------------------

IV – Program Specifications

A- Basic Information

1. Program title: Master of Science in Parasitology

2. Program type: Single Double Multiple

8

3. Faculty: Faculty of Medicine, Ain Shams University

4. Departments: Parasitology Department

5. Assistant coordinator

Prof. Dr.

6. Coordinator

Prof. Dr.

Head of the Parasitology Department

7. Last date of program approval: July 2009

B- Professional Information:

1. Program aims:

a) Providing broad-based knowledge and understanding of Medical

Parasitology including Helminthology, Malacology, Protozoology and

Medical Entomology.

b) Providing practical skills and experience in the laboratory diagnosis of

parasitic infections.

c) Providing the candidates with the basics of microbiology and

immunology.

d) Providing the students with basics of protein and nucleic acids structure,

functions and manipulations.

e) Providing knowledge and understanding in research methodology and

ethics, epidemiology, study planning, and data reporting, analysis and

presentation.

f) Motivating skills of working in team, personal communication, colleagues

and staff respect, and continuous self learning and improvement.

g) Providing the candidates with the basics of quality assurance in

parasitology laboratory

h) Providing knowledge and understanding of the ethics of medical

parasitology profession.

i) Providing knowledge and understanding of the research methodology and

ethics.

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the program the candidate will be able to:

a1) Show knowledge and understanding of the basics of microbiology and

immunology.

a2) Show knowledge and understanding of the basics of biochemistry

especially those of energy pathways, cell cycle, proteins and nucleic

acid structures, functions and manipulations.

a3) Show knowledge and understanding of the basic concepts epidemiology.

a4) List the geographical distribution, final habitat, definitive, intermediate,

and reservoir (host)s of parasites.

9

a5) Recognize the parasites according to their morphology and microscopic

features.

a6) Understand the biology of parasites.

a7) Understand the pathogenesis and clinical manifestations of parasitic

infections.

a8) Describe the methods used for the diagnosis of parasitic infections.

a9) Prescribe drugs for treating different parasites, including dosage and side

effects.

a10) Show awareness of epidemiological factors responsible for the

prevalence of parasitic infections.

a11) Understand the basics of quality assurance in parasitology laboratories.

a12) Understand the ethics of the medical parasitology profession.

a13) Understand the the basis of research methodology and ethics.

b. Intellectual capabilities:

By the end of the program the candidate will be able to:

b1) Classify microorganisms according to their morphologic features,

chemical and genomic structures.

b2) Correlate the type of the immune response to the type of the

microorganism.

b3) Formulate protocols for purification of proteins and DNA from

miccorganisms.

b4) Differentiate between and compare the diagnostic and infective stages of

parasites.

b5) Predict and correlate the clinical manifestations with pathogenesis and

pathology.

b6) Analyze a case history of a suspected parasitic infection to reach a

provisional diagnose, give differential diagnosis, and recommend further

investigations to reach the final diagnosis.

b7) Combine different drugs for treating parasitic infections according the

infectious status, presence of complications and drug resistance.

b8) Design plans for prevention and control of parasitic infections and control

of medically important arthropods and snails.

b9) Plan for continous self improvement.

b11) Carry out, critically evaluate, and write up an original research project.

(MSc. Thesis).

c. Professional and practical skills:

By the end of the program the candidate will be able to:

c1) Apply safe laboratory protocols

c2) Prepare sterile media, buffers and reagents.

10

c3) Isolate, maintain and culture microorganisms including parasites in the

laboratory.

c4) Prepare standardized antigens of micoorgansms including parasites.

c5) Identify microogansis based on their biochemical and molecular structurs.

c6) Examine urine, stool, blood and other body fluids for parasites using

direct and concentration techniques efficiently and correctly and within

appropriate time.

c7) Apply the proper immunologic assays for the diagnosis of parasitic

infection.

c8) Report the result without delay.

c9) Recommend further investigation.

c10) Collect and analyze data using appropriate statistical and diagrammatic

methods.

c11) Design suitable questionnaires to investigate the epidemiological factors

influencing the prevalence of parasitic diseases, and apply proper

preventive and control measures.

d. General and transferable skills:

By the end of the program the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

d3) Show willing and motivations for self learning and improvement of the

department and faculty.

d4) Participate in community services via medical and social campaigns.

d5) Search and retrieve required scientific data via the world wide web and

other electronic resources.

3. Academic standards: (Benchmarks)

Benchmarks (MSc of Medical Parasitology-London School of Hygiene and

Tropical Medicine-London University).

4. Curriculum structure and contents:

4a- Program duration: 4 Semesters (2 years)

4b- Program structure

Curricula

Course Code Credit

hours

Faculty

Requirements

Basics of Research Tools

First Part

Microbiology and Immunology PAR6001 2

Biochemistry PAR6002 2.5

Public Health PAR6003 1.5

11

MSc. Thesis 6

Second Part

Medical

Parasitology

Helminthology PAR6004 8

Protozoology and Entomology PAR6005 9

Elective Courses

(Only one course

of)

Molecular biology E6002

1 Immunology E6031

Vector biology E6032

Biostatistics E6009

Log Book 6

Total 36

5. Program courses:

Code

No.

Course Title Level/year of

program

No. of hours/week

Lect. Lab.

PAR6001 Microbiology and

Immunology

Semester 1

2

(10 weeks)

2

(10 weeks)

PAR6002 Biochemistry 2

(15 weeks)

2

(8 weeks)

PAR6003 Public health 1

(15 weeks)

1

(15 weeks)

PAR6004

Helminthology:

Trematoda and

Cestoda

Semester 2

3

(15 weeks)

4

(15 weeks)

One of

E6002

E6031

E6032

E6009

Molecular biology

Immunology

Vector biology

Biostatistics

Semester 2

1

(10 weeks)

1

(10 weeks)

PAR6004

Helminthology:

Nematoda

Semester 3 3

(15 weeks)

2

(15 weeks)

PAR6005 Protozoology

- Sarcomastigo

phora

- Ciliophora

Semester 3 2

(15 weeks)

PAR6005

Protozoology

- Apicomplexa

- Microspora

Medical

Entomology

Semester 4 2

(15 weeks)

2

(15 weeks)

2

(15 weeks)

2

(15 weeks)

6. Program admission requirements:

12

-يد الطالب للحصول على درجة الماجستير:( : يشترط لق٦) المادة عمى درجة البكالوريوس في الطب والجراحة مف إحدى جامعات جمهورية مصر - 1 أف يكوف حاصال

.العربية أو عمى درجة معادلة

.أف يكوف قد أمضى سنة التدريب )االمتياز( -2 .موافقة جهة العمؿ -3 ( 1الؾ األجهزة واستيفاء المستندات المطموبة في الممحؽ )تسديد الرسوـ ومصاريؼ التدريب واسته -4 .التفرغ لمدراسة لمدة فصميف دراسييف قبؿ دخوؿ امتحاف الجزء الثاني -5

-الماجستير األوراؽ التالية :ة يقدـ طالب اإللتحاؽ لدرج ( :1ملحق ) .طمب إلتحاؽ. 1 .شهادة البكالوريوس. 2

.شهادة اإلمتياز. 3

.ميالد أو مستخرج رسميشهادة ال. 4

.الموقؼ مف التجنيد. 5

.موافقة جهة العمؿ عمى التسجيؿ والتفرغ المطموب. 6

.صور فوتوغرافية جديدة 6عدد . 7

.بالنسبة لموافديف يقدـ الطالب موافقة السفارة ويحدد جهة تحمؿ النفقات كما يقدـ شهادة صحية. 8

7. Regulation for progression and program completion

يتـ التسجيؿ لمماجستير مرة واحدة في السػنة تبػدأ مػف أوؿ يوليػو حتػى أخػر أغسػطس عمػى أف تبػدأ (: 8مادة )الدراسػػة فػػي شػػهر أكتػػػوبر مػػف كػػؿ عػػػاـ ويجػػوز قبػػوؿ تسػػػجيؿ النػػواب والمعيػػديف والوافػػػديف فػػي الفتػػرة مػػػف أوؿ

لجػػػزء األوؿ إال بعػػػد إنقضػػػاء فتػػػرة الدراسػػػػة نػػػوفمبرحتى أخػػػر ديسػػػمبر عمػػػى أال يسػػػمح لهػػػـ بػػػػدخوؿ إمتحػػػاف ا .المطموبة

توزع الدراسة فى كؿ عاـ جامعى عمى فصػميف دراسػييف مػدة كػؿ منهمػا خمسػة عشػر اسػبوعا . يبػدا (: 9مادة )االوؿ فى أوؿ أكتوبر ويبدا الثانى فى منصؼ فبراير. مع تنظيـ فصػؿ دراسػى صػيفى مكثػؼ لمػدة سػتة اسػابيع

فصػؿ الدراسػى قبػػؿ اسػبوعيف مػف بدايتػال عمػى االقػػؿ بعػد إسػتيفاء الشػروط حسػب المقػػررات . و يػتـ التسػجيؿ لمسػاعات معتمػدة. ويجػوز لمطالػب تعػديؿ ٦المسجمة. والينبغى أف يزيد العبء الدراسى فى الفصػؿ الواحػد عػف

اب خػػالؿ المقػػررات خػػالؿ اسػػبوعيف مػػف بدايػػة الفصػػؿ الدراسػػى )بالحػػمؼ او االضػػافة(. كمػػا يجػػوز لػػال االنسػػح ستال اسابيع مف احد المقرارت دوف احتسابال راسبا فيال.

: مدة الدراسة لمحصوؿ عمى درجة الدبمـو أو الماجستير هى أربعة وعشروف شهرا )أربعة ( 11المادة )

13

فصوؿ دراسية( عمى جزئيف يجتاز فيها الطالب برنامجا تدريبيا متكامال طبقا لمساعات المعتمدة الموضحة وال يسمح لال بدخوؿ اإلمتحاف قبؿ األنشطة اب الرابع ويستوفى خاللها المطموب منة فى كتيب متابعة بالب

إستيفاء ثالثة أرباع المطموب منة في كؿ جزء مف البرنامج. يجتاز بعدها الطالب واحد الدبموـ والماجستير الجزء األوؿ فصؿ دراسي فيمدة الدراسة ( :12المادة )

يشترط النجاح فيال بالكامؿ لإلنتقاؿ لمدراسة في الجزء الثاني. والجزء الثاني يتطمب تفرغ الطالب إمتحانا وال .بأحد المستشفيات أو المراكز المعتمدة مف الكمية لمدة فصميف دراسييف لمتدريبيقـو الدارس لدرجة الماجستير بتسجيؿ موضوع الرسالة عمى شكؿ بحث نظري أو عممي بعد ( : 13مادة )

عمى األقؿ عمى مف التسجيؿ ستيفاء فصؿ دراسي واحد عمى األقؿ ويجوز أف يناقش رسالتة بعد ستة شهور إلها صأف يكوف قد نجح فى مقررات الجزء األوؿ بالكامؿ وقبؿ دخوؿ إمتحاف الجزء الثاني وال يخص

ت. درجاالرسالة ومتطمبات الكمية قبؿ يقوـ دارس الماجستير بإستيفاء متطمبات الجامعة قبؿ تسجيؿ ( : 14مادة )

متطمبات الجامعة هي الحصوؿ عمى شهادة التويفؿ في المغة اإلنجميزية و .دخوؿ إمتحاف الجزء الثانيدرجة عمى األقؿ( ومتطمبات الكمية هي حضور دورات معتمدة مف لجنة الدراسات العميا 450بمجموع )

طبى أو بإجتياز إختبارات خاصة تحددها المجنة. واإلحصاء ال مناهج البحث العممىبالكمية في مجاؿ يجوز لمحاصميف عمى درجة الدبمـو إستكماؿ درجة الماجستير بتسجيؿ رسالة الماجستير خالؿ (: 15مادة )

أربع سنوات مف الحصوؿ عمى درجة الدبموـ ويحصؿ عمى الدرجة بعد إستيفاء متطمبات الجامعة والكمية ومناقشة الرسالة بنجاح.

الساعات المعتمدة لدراسة الدبمـو والماجستير ثالثوف ساعة معتمدة عمى األقؿ يخصص منها (: 16دة )ماوتشمؿ ست ساعات عمى األقؿ لمجزء األوؿ وثماني عشرة ساعة عمى متابعة األنشطة ست ساعات لكتيب

ويضاؼ إليها ست ساعات لمرسالة فى الماجستير. األقؿ لمجزء الثاني القيد لدرجة الماجستير أربع سنوات. مدة(: 22مادة )درجة لمجزء األوؿ. 300درجة منها 1200مجموع درجات االمتحاف النهائى لمماجستير (: 24مادة )

درجة لمماجستير. 300 مى التراكمى بما يوازىصويضاؼ اليها المعدؿ الفإمتحاف الدور الثاني فى أبريؿ يعقد إمتحاف الدور األوؿ في أكتوبر ونوفمبر مف كؿ عاـ ويعقد (: 25مادة )

ومايو مف كؿ عاـ. مف الدرجة % 60يكوف النجاح فى كؿ مادة مف الدبموـ العالى والماجستير بعد الحصوؿ عمى (: 26مادة )

.% 05عمى اف ال تقؿ درجة التحريرى عف الكمية لكؿ لجاف المادةعتػػػد (: فػػػى حالػػػة إسػػػتنفام مػػػدة القيػػػد يمكػػػف لطالػػػب الدراسػػػات العميػػػا إعػػػادة التسػػػجيؿ مػػػرة أخػػػرى وال ي29)ماااادة

بالنجاح فى الجزء األوؿ أو الرسالة ويجب إعادتهما.

14

8. Assessment Schedule and Weighing of Assessments

Item Mark

Po

ints

GP

A

sco

re

ةطان

اننح

حا

Stu

den

t

sta

te

During

semester

End of

semester Total

First semester (If present) 100 300 400

Second semester 100 50 150

Third semester 100 100

Fourth

semester

Final exam

Written

850 850

Oral

Practical

/Clinical

Total 300 1200 1500

عه انىجه انتان:يهحىظح: تعادل درجاخ انطانة طثقا نهقاط

قاط 4 : % فأكثز 00 -1 A

قاط 3663 : % 00% حت أقم ي 85ي -2 A-

قاط 3633 : %85% حت أقم ي 80ي - 3 B+

قاط 3600 : %80% حت أقم ي 35ي - 4 B

قاط 2663 : %35% حت أقم ي 30ي - 5 B-

قاط 2633 : %30% حت أقم ي 65ي - 6 C+

قاط 2600 : %65% حت أقم ي 62ي -3 C

قاط 1663 : %62% حت أقم ي 60ي - 8 C-

F صفز : %60أقم ي - 0

درجح نهجشء6 300درجح يها 1200يجىع درجاخ االيتحا انهائ نهدتهىو انعان واناجستيز

. 300ه انتزاك تا يىاسيصنعدل انفويضاف إنيها ا

( عه أساص يجىع حاصم ضزب قاط كم يقزر يضزوتا فى GPAويتى حساب انعدل انفصه )

عدد ساعاته انعتدج يقسىيا عه انساعاخ انعتدج نهقزراخ انت درسها انطانىة فى انفصىم اندراسى6 كىا

أساص يجىع حاصم ضىزب انقىاط انتى حصىم عهيهىا ( عهCGPAانعدل انتزاك نهطانة ) يتى حساب

انطانة ف كم يقزر يضزوتا ف عدد ساعاته انعتدج يقسىيا عه يجىع انساعاخ انعتدج انكهيح6

يتى اإلعادج ف انىادج أو واناجستيزف اندتهىو أقزراخ انف حانح انزسىب ف يادج أو يجىعح ي

انتقديز انفعه انذي يحصم عهيه ف أول إعىادج فقىظ أيىا إتا تكىزر رسىىتح فيحسىة ويتى حساب انجىعح فقظ6

قاط اي 1663)اي فقظ 60% نه عد انجاح تقديز-

C).

1. Course specification for Microbiology and Immunology

15

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: major

Department offering the program: Parasitology Department

Department offering the course: Medical Microbiology and Immunology

Academic year / Level: First year/ First semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Microbiology and Immunology Code: PAR6001

Credit Hours: 2

Lecture: 2hours/w (10w) Practical: 2hours/w (10w) SDL

Total: 20 theoretical and 20 practical hours.

Coordinator

Head of the department

B - Professional Information

1- Course Aims:

To introduce the candidate to the basics of microbiology and immunology

including:

a) The nature of viruses, bacteria, parasites and fungi and basic criteria used

in the classification/taxonomy of these micro-organisms.

b) Microbial genetics.

c) Microbial physiology including growth requirements and metabolism.

d) Methods of sterilization and disinfection.

e) Virulence factors of microorganisms.

f) Basics of the immunity to microorganisms including types of the

immunity; structure, function and organization of the immune system.

g) Types of the immune response and phases of the adaptive immune

response.

h) Basics of cellular and humoral immune response.

2- Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Identify eukaryotic and prokaryotic cell structure.

a2) Describe the basic structure and function of components of the bacterial

cell.

a3) Understand classification systems and typing of microorganisms.

a4) Understand the basics of bacterial growth, physiology, energy production.

a5) Understand the basics of microbial genetics including genomic

organization, DNA conjugation, transformation, and transduction.

16

a6) Describe mechanisms and applications of different methods used for

sterilization and disinfection.

a7) Understand the virulence factors responsible for microbial pathogenecity.

a8) Understand the nature and type of immunity.

a9) Understand the organization (anatomy) and function of the immune

system.

a10) Understand the phases and characteristics of the adaptive immune

response.

a11) Understand the basics of nosocomial and laboratory acquired infections.

b) Intellectual skills

By the end of the course the candidate will be able to:

b1) Differentiate between prokaryotes and eukaryotes.

b2) Compare the different types of microscopes.

b3) Interpret the tests for monitoring the efficiency of sterilization process.

b4) Differentiate between the types of immunity and immune response.

b5) Formulate a systematic approach for laboratory diagnosis of common

infectious clinical conditions using the most appropriate and cost-

effective tool leading to the identification of the causative organism.

c) Professional and practical skills:

By the end of the course, candidate will be able to:

c1) Apply safe laboratory protocols.

c2) Prepare slides for microbiological examination.

c3) Stain microorganisms using simple and differential techniques.

c4) Identify the microorganisms according to their shape, arrangement,

staining and biochemical and culture characteristics.

c5). Apply the proper method for sterilization of different media.

c6) Use properly aseptic techniques for the transfer and handling of

microorganisms and instruments.

c7) Apply the proper method of disinfection.

c8) Isolate microorganisms form different specimens.

c9) Estimate the number of microbes in a sample using serial dilution

techniques.

c10) Perform some biochemical tests for the identification of bacteria.

c11) Perform in vitro susceptibility and resistance of bacteria of antibiotics

using disc diffusion and dilution methods.

c12) Apply the proper methods of prevention of laboratory acquired

infections.

d) General and transferable skills:

By the end of the course the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

17

d3) Show willing and motivations for self learning and continuous

improvement.

d4) Represent data efficiently.

d5) Search and retrieve required scientific data via the world wide web

and other electronic resources.

3- Course contents:

Topics No of hours

Lecture Practical

2hours/w 2 hours/week

Bacterial cell structure 2

Classification and typing of

microorganisms.

1

Bacterial growth, physiology 2

Bacterial genetics 2

Control of microbial growth 4

Antimicrobials 2

Host parasite relationship 2

Nosocomial acquired infections 2

Tissues and cells of the immune

system

2

Types of immune response

(innate and adaptive)- cellular

immune response

2

Antigens and immunogenicity 1

Humoral immune response

(Immunoglobulins and

complement

2

Cell mediated immunity and

cytokines

2

Lab Safety 1

Types of microscopes 1

Collection of pathological

specimens

2

Isolation of microorganisms

from different specimens

2

Estimation of number of

microbes in samples

2

Biochemical tests for

identification of isolates

2

18

Antimicrobial susceptibility

tests

2

Basic immunological techniques

as agglutination, precipitation

and complement fixation.

2

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills.

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution:

Written: 60 Marks Practical: 30 Marks Oral: 10 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Jawetz, Melnick, and Adelberg’s medical microbiology, 24th

Edition,

2007, Brooks J.F., Carroll K.C., Butel J.S., and Morse S.A.

b) Medical Microbiology, 17th

edition, 2007, Greewood D., Slack R.,

Peutherer J., and Barer M.

c) Mackie and MaCatney Pratical Medical Microbiology,14th

Edition, 1996, Collee J.G., Fraser A.G., Marmion B.P., and Simmons

A.

d) District Laboratory Practice in Tropical Countries, part 2, 2000,

Cheesbrough M., Cambridge Press.

e) Microbiology: An Introduction (10th Edition) by Gerard J. Tortora,

Berdell R. Funke, and Christine L. Case.

f) Bailey & Scott's Diagnostic Microbiology.

5.3- Recommended Books

a) Microbiology: An Introduction (10th Edition) by Gerard J. Tortora,

Berdell R. Funke, and Christine L. Case.

b) District Laboratory Practice in Tropical Countries, part 2, 2000,

Cheesbrough M., Cambridge Press.

5.4- Periodicals, Web Sites, etc

a) Center for disease control and prevention:www.cdc.gov

b) Medscape:www.medscape.com/infectious diseases

c) Pubmed:www.ncbi.nlm.nih.gov/pubmed

d) World health organization: www.who.int/en

19

2. Course specification for Biochemistry

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: major

Department offering the program: Parasitology Department

Department offering the course: Biochemistry

Academic year / Level: First year/ First semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Biochemistry Code: PAR6002

Credit Hours: 2.5

Lecture: 2hours/w (15w) Practical: 2hours/w (15w) SDL

Total: 30 theoretical and 15 practical hours

Coordinator

Head of the department

B- Professional Information

1. Course Aims:

To introduce the candidate to the basics of proteinss and nucleic acids

structure, function and manipulations including

a) Basic structure and functions of proteins.

b) Basic structure, functions and organization of nucleic acids.

c) Cell cycle.

d) DNA replication, transcription and translation.

e) Basic structure and function of the biological membranes.

f) Proteins and nucleic acid manipulations.

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Describe the basic structure and functions of proteins.

a2) Describe the basic structure, function and organization of nucleic acids.

a3) Understand major biochemical pathways of energy production.

a4) Understand the structure, mechanism of action of enzymes and their

application in diagnosis of infections and diseases.

a5) Understand the cell cycle.

a6) Understand the basics of purification and separation of proteins.

a7) Understand the basics of chromatography and electrophoresis.

a8) Understand the basic of DNA manipulations as purification, synthesis and

sequencing.

a9) Understand the principle of spectrophotometry.

20

a10) Understand the basics of buffering and pH.

b) Intellectual skills

By the end of the course the candidate will be able to:

b1) Compare the structures of DNA and RNA.

b2) Predict the amino sequence of a given codons.

b3) Estimate the amount of ATP produced during aerobic and anaerobic

respiration.

b4) Formulate protocols for the purification of proteins and DNA from some

parasitic stages.

c) Professional and practical skills:

By the end of the course, candidate will be able to:

c1) Apply safe laboratory protocols.

c2) Prepare buffers of defined pH and molarities.

c3) Separate proteins by SDS polyacrylamide gel electrophoresis.

c4) Measure protein concentration by Lowery and Bradford techniques.

c5) Measure concentrations of some analytes by spectrophotometer.

c5) Extract DNA.

c6) Measure the concentration of DNA in samples.

d) General and transferable skills:

By the end of the course the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

d3) Show willing and motivations for self learning and continuous

improvement.

d4) Represent data efficiently.

d5) Search and retrieve required scientific data via the World Wide Web

and other electronic resources.

3- Course contents:

Topics No of hours

Lecture Practical

Bio-molecules (Carbohydrates,

lipids and proteins)

2

Structure and Functions of Proteins 4

- Structure and Functions of

Nucleic acids

- DNA replication and transcription

- Genes to proteins

6

Nucleic acid manipulation:

amplification, sequencing,

restriction analysis, blotting, etc.

4

21

Cell cycle 2

Genomic organization, genetic code 4

Enzymes: structure, function,

isoenzymes, etc.

4

Bioenergetics 2

Biological membranes 2

Chromatography 2

Electrophoresis 2

Protein purification and separation 4

Principles of spectrophotometry 2

Molarity, molality, hydrogen ion

concentration, pH, buffering,

preparation of buffers of certain pH

and molarity

4

Measuring of protein and DNA

concentration

2

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills

Marks distribution:

Written: 75 Marks Practical: 37.5 Marks Oral: 12.5 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Biochemistry (3rd Edition) (Biochemistry (Mathews/ Addison-

Wesley).

b) Principles of Biochemistry (4th Edition) by Robert Horton, Laurence

A Moran, Gray Scrimgeour, and Marc Perry.

c) Harper's Illustrated Biochemistry, 28th Edition (LANGE Basic

Science) by Robert Murray, Victor Rodwell, David Bender, and

Kathleen M. Botham.

d) Molecular Biology of the Cell by Bruce Alberts, Alexander Johnson,

Julian Lewis, and Martin Raff.

e) Molecular Cell Biology by Harvey Lodish, Arnold Berk, Chris A.

Kaiser, and Monty Krieger.

5.3- Recommended Books

22

a) Harper's Illustrated Biochemistry, 28th Edition (Lange Basic Science)

by Robert Murray, Victor Rodwell, David Bender, and Kathleen M.

Botham

b) Molecular Biology of the Cell by Bruce Alberts, Alexander Johnson,

Julian Lewis, and Martin Raff 5.4- Periodicals, Web Sites, etc

5.4- Periodicals, Web Sites, etc

a) Pubmed: http://www.ncbi.nlm.nih.gov/

b) DNA from the beginning: http://www.dnaftb.org/dnaftb/

23

3. Course specification for Public Health

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: major

Department offering the program: Parasitology Department

Department offering the course: Community, Environmental and Occupational

Medicine

Academic year / Level: First year/ First semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Public Health Code: PAR6003

Credit Hours: 1.5

Lecture: 1 hour/w (15w) Practical: 1 hour/w (15w) SDL

Total: 15 theoretical and 15 practical hours

Coordinator

Head of the department

B - Professional Information

1. Course Aims:

To provide an introduction to the basic concepts research methodology and

epidemiology.

Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Understand the basics of disease surveillance.

a2) Understand and apply measures of disease incidence (risk, rate and odds)

and prevalence, and measures of effect (e.g. relative and absolute risk).

a3) Demonstrate understanding of the basic principles underlying different

study designs, including cross-sectional, cohort, case-control and

intervention studies.

a4) Understand the concepts of estimation of sensitivity, specificity and

predictive values.

a5) Understand the basics of clinical trials.

a6) Know the epidemiological factor responsible for the prevalence of

parasitic diseases in Egypt.

a7) Understand the basics of questionnaire design.

b) Intellectual skills

By the end of the course the candidate will be able to:

b1) Assess strengths and limitations of different study designs.

b2) Identify problems caused by random errors, bias and confounding in

interpreting epidemiological data.

24

b3) Estimate sensitivity, specificity and predictive values.

c) Professional and practical skills:

By the end of the course, students will be able to:

c1) Measure disease incidence and prevalence of parasitic diseases.

c2) Design the proper study for investigation of parasitological problems.

c3) Estimate sensitivity, specificity and predictive values for

parasitological laboratory tests.

c4) Design suitable questionnaires to investigate the epidemiological

factors influencing the prevalence of parasitic diseases.

c4) Apply the proper measures to control and prevent parasitic infections.

d) General and transferable skills:

By the end of the program the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

d3) Show willing and motivations for self learning and continuous

improvement.

d4) Represent data efficiently.

d5) Search and retrieve required scientific data via the World Wide Web

and other electronic resources.

3- Course contents:

Topics No of hours

Lecture Practical

Aim of Research 1

Research Design

- Sampling

- Cohort studies

- Cross sectional studies

- Case control studies

- Intervention studies

2

1

1

1

1

4

Research tools

Sensitivity and specificity

Questionnaires

2

2

2

4

Measuring of disease frequency 2 2

Surveillance for disease

2 3

25

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills.

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution:

Written: 45 Marks Practical: 22.5 Marks Oral: 7.5 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Modern Epidemiology by Kenneth J Rothman, Sander Greenland,

and Timothy L Lash.

b) Epidemiology for Public Health Practice by Robert Friis and Thomas

Sellers.

c) Epidemiology: An Introduction by Kenneth J. Rothman

d) Essentials of Epidemiology in Public Health, Second Edition by Ann

Aschengrau; George R. Seage.

e) Introduction to Epidemiology by Ray M. Merril and Thomas C.

Timmreck.

5.3- Recommended Books (Text Books)

Handbook of Epidemiology. Springer 2005

5.4- Periodicals, Web Sites, etc

a) http//.www. Epidemiolog.net

26

4. Course specification for Parasitology

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: Major

Department offering the program: Parasitology Department

Department offering the course: Parasitology Department

Academic year / Level: First and second years/ second through fourth semesters.

Date of specification approval ……………………………………..

A- Basic Information

Title: Helminthology Code: PAR6004

Credit Hours: 8

Lecture: 3 hour/w (30w) Practical: 2 hour/w /(30w) SDL

Total: 90 theoretical and 60 practical hours

Title: Protozoology and Entomology Code: PAR6005

Credit Hours: 9

Lecture: 3 hour/w (30w) Practical: 3 hour/w (30w) SDL

Total: 90 theoretical and 90 practical hours

Coordinator

Head of the department

B - Professional Information

1. Course Aims:

a) Providing a broad-based knowledge and understanding of Medical

Parasitology including Helminthology, Malacology, Protozoology and

Medical Entomology.

b) Providing practical skills and experience in the laboratory diagnosis of

parasitic infections.

c) Providing knowledge and understanding in research methodology and

ethics, study planning, and data reporting, analysis and presentation.

d) Motivating skills of working in team, personal communication, colleagues

and staff respect, and continuous self learning and improvement.

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the program the candidate will be able to:

a1) Locate the geographical distribution of parasites in the world and in

Egypt.

a2) Name the final habitat, definitive, intermediate, and reservoir hosts of the

parasite.

a3) Recognize the parasites according to their morphology and microscopic

features.

27

a4) Recognize medically important arthropods according to their morphologic

features.

a5) Identify the snails acting as intermediate hosts of trematodes studied

according to their external features and morphology of the shells.

a6) Understand the physiology (reproduction, nutrition, respiration, secretion,

excretion, locomotion) of parasites and bionomics of medically

important arthropods.

a7) Understand the ecology of snails including physical factors (water level

and movement, nature of water body, vegetation in water, depth of water

and altitude), sunlight and shade, temperature, and chemical factors

(Oxygen, calcium, pollution).

a8) Describe and draw the life cycle of parasites.

a9) Mention the modes of transmission and infective stage(s).

a10) Draw labeled diagrams of diagnostic and infective stage(s).

a11) Understand the pathogenesis, pathology of parasitic infections.

a12) Tell the clinical manifestations of parasitic infections.

a13) Tell the medical importance of arthropods including the modes and ways

of transmission.

a14) Describe the methods used for the diagnosis of parasitic infections.

a15) Prescribe drugs for treating different parasites, including dosage and side

effects.

a16) Show awareness of epidemiological factors responsible for the

prevalence of parasitic infections.

a17) Understand methods of prevention and control parasitic infections.

a18) Understand methods of control of medically important arthropods.

a19) Understand methods of control of medically important snails.

b) Intellectual capabilities:

By the end of the program the candidate will be able to:

b1) Classify the parasites according to their morphologic and microscopic

feature.

b2) Classify medically important arthropod and snails according to their

morphologic features.

b3) Construct attractive explanatory drawing for life cycle, infective stage and

modes of infection.

b4) Differentiate between and compare the diagnostic and infective stages of

parasites.

b5) Predict and correlate the clinical manifestations with pathogenesis and

pathology.

b6) Explore the differential diagnosis on the basis of suspected clinical

manifestations and recommend the relevant further investigations.

28

b7) Evaluate the infectious status; acute, chronic, or latent, from the given

manifestations and the available diagnostic tests.

b8) Combine different drugs for treatment according the infectious status,

presence of complications and drug resistance.

b9) Advise people travelling endemic areas, concerning prophylactic health

measures and use of prophylactic drugs.

b10) Design plans for prevention and control of parasitic infections and

control of medically important arthropods and snails.

b11) Carry out, critically evaluate, and write up an original research project.

(MSc. Thesis).

c) Professional and practical skills:

By the end of the program the candidate will be able to:

c1) Apply safety measures in the parasitology laboratory.

c2) Maintain the equipments in the parasitology laboratory.

c3) Respect patients and their relatives and communicate with them

efficiently for full history taking.

c4) Examine urine, stool, blood and other body fluids for parasites using

direct and concentration techniques efficiently and correctly and within

appropriate time.

c5) Differentiate between parasitic stages and artifacts that might be present

in samples.

c6) Honestly reporting the result without delay.

c7) Report results for seniors if required.

c8) Recommend further investigation.

c9) Contact treating physician for inquiring information that might be helpful

for the laboratory diagnosis as well as to report urgent cases.

c10) Apply the appropriate stain needed for each specimen.

c11) Preserve and culture faecal and other parasitological specimens.

c12) Preserve worms, protozoa and insects and prepare microscopic slides.

c13) Identify, classify and draw helminthes, protozoa, and snails and

arthropod of medical importance.

c14) Apply and interpret the appropriate immunological test for diagnosis of

parasitic infections.

c15) Collect and analyze data using appropriate statistical and diagrammatic

methods.

d) General and transferable skills:

By the end of the program the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

29

d3) Help colleagues in pursuing their research activities.

d4) Show willing and motivations for self learning and continuous

improvement.

d5) Show willing and motivation for improvement of the department and

faculty.

d6) Show competence, efficiency and honesty in all assigned scientific and

social activity.

d7) Represent data efficiently.

d8) Work in challenging and competitive environment.

d9) Search and retrieve required scientific data via the World Wide Web and

other electronic resources.

d10) Show interest in local health problems.

d11) Participate in community services via medical and social campaigns.

3. Course content:

A) Theory

Course title Hours

1. INTRODUCTION AND CLASSIFICATION

OF PARASITES

- Historical Perspectives

- Host-Parasite relationship

- Clinical manifestations of parasitic diseases

- Prevalence and transmission of parasitic infections

- Taxonomy and classification

6

2. HELMINTHS

PHYLUM PLATYHELMINTHES

CLASS: TREMATODA

Introduction

Family: Fasciolidae

Family: Opisthorchiidae

Family: Heterophyidae

Family: Echinostomatidae

Family: Dicroceliidae

Family: Gastrodiscidae

Family: Schistosomatidae

Family: Troglotrematidae

MEDICAL MALACOLOGY

CLASS: CESTOIDEA

Introduction

Family: Diphyllobothriidae

2

4

2

1

1

1

1

6

2

4

2

3

6

30

Family: Taeniidae

Family: Dilepididae

Family: Hymenolipedidae

2

2

PHYLUM NEMATHELMINTHES

Introduction

CLASS: ADENOPHOREA

Family: Trichuridae

Family: Capillaridae

Family: Trichinellidae

Family: Dioctophymatidae

CLASS: RHABDITEA Family: Strongyloididae

Family: Ancylostomidae

Family: Trichstrongylidae

Family: Angiostrongylidae

Family: Ascarididae

Family: Oxyuridae

Family: Dracunculoidae

Family: Gnathostomatoidae

Family: Anisakidae

Family: Physalopteridae

Family: Gongylonematidae

Family: Filariidae

Family: Onchoceridae

Family: Thelaziidae

3

2

2

4

2

3

3

1

1

3

2

2

2

1

1

1

3

2

1

3. Immune Response to Helminthes 6

4. PROTOZOA

Introduction

PHYLUM SARCOMASTIGOPHORA

CLASS: RHIZOPODA

Family: Endamoebidae

Family: Acanthamoebidae

Family: Vahlkampfiidae

CLASS: ZOOMASTIGOPHORFA Family: Trypanosomatidae

Genus: Leishmania

Genus: Trypanosoma

Family: Bodonidae: Genus: Retortamonas

Family: Tetramitidae Genus: Enteromonas

3

6

15

31

Family: Hexamitidae Genus: Giardia

Family: Chilomastigidae Genus: Chilomastix

Family: Monocercomonadidae Genus: Dientamoeba

Family: Trichomonadidae Genus: Trichomonas

PHYLUM CILIOPHORA Family: Balantidiidae

PHYLUM APICOMPLEXA (SPOROZOA)

SUBCLASS: COCCIDIA

SUBORDER: EIMERIINA Family: Cryptosporidiidae

Family: Eimeriidae (Eimeria and Isospora)

Family: Sarcocystidae (Toxoplasma and Sarcocystis)

SUBORDER: HAEMOSPORINA Family: Plasmodiidae

SUBCLASS PIROPLASMEA Family: Babesiidae

PHYLUM MICROSPORA Family: Nosematidae

1

6

6

2

3

5. Immune Response to Protozoa 6

6. Nosocomial parasitic infections: 3

7. Parasitic infections of compromised host 3

8. Symptoms and sign sof parasitic infections

6

9. MEDICAL ENTOMOLOGY

Introduction

CLASS INSECTA

SUBCLASS: ENDOPTERYGOTE INSECTS (Holometabola) I. ORDER: DIPTERA [Mosquitoes and Flies]

A) SUBORDER: NEMATOCERA

Family: Psychodidae (Blood sucking sand flies and non-biting moth-

flies)

Family: Anisopodidae (Window-gnats) (Facultative accidental

intestinal myiasis)

Family: Culicidae (Mosquitoes)

2

4

32

Genus: Anopheles Genus: Culex Genus: Aedes

Family: Simuliidae (Black flies, Buffalo gnat)

Family: Ceratopogonidae (Biting midges)

Family: Tipulidae (Facultative accidental intestinal

myiasis)

B) SUBORDER: BRACHYCERA

1. Infraorder: Tabanomorpha

Family: Tabanidae

Genus: Tabanus (Horse-flies - Myiasis)

Genus: Chrysops (Deer-flies)

2. Infraorder: Muscomorpha (Cyclorrapha)

a) Section: Aschiza

Family: Syrphidae (Hover-flies, Drone-flies)

Genus: Erystalis

b) Section: Schizophora:

Family: Glossinidae

Family: Muscidae (Myiasis)

Family: Fanniidae (Myiasis)

Family: Oestridae (Head maggots-Myiasis)

Family: Calliphoridae (Blow flies-Myiasis)

Family: Sarcophagidae (Flesh flies- Myiasis)

Family: Gastrophilidae

Family: Hypodermatidae

Family: Cuterebrinae (Skin bot flies-Myiasis)

II. ORDER: SIPHONEPTERA [Fleas]

Family: Pullicidae

Family: Tungidae

SUBCLASS EXOPTERYGOTA (Hemimetabola)

I. ORDER : PHTHIRAPTERA

SUBORDER: ANOPLURA (Lice: biting lice and sucking lice)

Family: Pediculidae

Family: Phthiridae

II. ORDER: HEMIPTERA

SUBORDER: HETRROPTERA

Family: Cimicidae

Family: Cacodmidae

Family: Reduviidae

CLASS ARACHNIDA

3

2

3

6

33

SUBCLASS: ACARI

I. ORDER: PARASITIFORMES

A) SUBORDER: IXODIDA (Metastigmata: TICKS)

Family: Ixodidae (Hard Ticks)

Family: Argasidae (Soft Ticks)

B) SUBORDER: GAMASIDA (Mesostigmata: MITES)

Family: Dermanyssidae

Family: Macronyssidae

Family: Laelapidae

II.ORDER: ACARIFORMES A) SUBORDER: ACARIDIDA (Astigmata)

Family: Sarcoptidae (Sarcoptes scabiei)

Family: Pyroglyphidae (House dust mites)

Family: Acaridae

Family: Glycyphagidae

B) SUBORDER: ACTINEDIDA (Prostigmata)

FFaammiillyy: Demodicidae

Family: Trombiculidae

SUBCLASS ARANAE (SPIDERS)

SUBCLASS SCORPIONES (SCORPIONS)

CLASS CRUSTACEA

I. SUBCLASS COPEPODA

ORDER: EUCOPEPODA

II. MALACOSTRACA ORDER: DECAPODA (Cray fish, Lobster, Crabs)

Basics of vector biolog

2

4

34

B) Practical

Course title Hours

Unit 1

Safety measures in parasitology laboratory

Equipping of parasitology laboratory

Maintenance, cleaning of microscopes, balances

Preparation of buffers, stains and reagents

6

Unit 2

Stool Examination

1. Collection, preservation and transport of faecal specimens

2. Macroscopic examination

3. Microscopic examination

- Direct smear method

- Stool concentration techniques

- Reporting of the results

- Quality control

- Egg counting techniques (Kato-katz, Stoll's)

- Stool staining techniques (Trichrome, Iron hematoxylin,

Modified Trichrome, Modified Ziehl-Neelsen)

4. Culture of larval stage of Nematodes

5. Stool artifact

14

Unit 3

Examination of Blood for Parasites

1. Collection of blood specimens

2. Preparation of thin and thick blood films

3. Staining of blood films

4. Reporting of the results

10

Unit 4

Examination of other specimens

Urine, CSF, sputum, LN, skin biopsies, and vaginal washout

6

Unit 5

Preparation of permanent microscopic slides (helminths, protozoa,

arthropods)

4

Unit 6

Serodiagnosis of parasitic infections (IHA, ELISA, IB)

6

35

Unit 7

Examination of permanent microscopic slides and specimens

Helminths

CLASS: TREMATODA

Family: Fasciolidae (Fasciola gigantica, F. hepatica, Fasciolopesis

buski)

Family: Opisthorchiidae (Clonorchis sinensis, Opisthorchis felineus)

Family: Heterophyidae (Heterophyes heterophyes, Metagonimus

yokogawai

Family: Echinostomatidae (Echinostoma ilocanum)

Family: Dicroceliidae (Dicrocoelium dendriticum)

Family: Gastrodiscidae (Gastrodiscoides homonis)

Family: Schistosomatidae (Schistosoma haematobium,

S. mansoni, S. japonicum)

Family: Troglotrematidae (Paragonimus westermani)

CLASS: CESTOIDEA

Family: Diphyllobothriida (Diphyllobothrium latum, Spirometra

mansonoides)

Family: Taeniidae

Taenia saginata, Taenia solium

Echinococcus granulosus, Echinococcus multilocularis

Family: Dilepididae (Dipylidium caninum)

Family: Hymenolipedidae (Hymenolepis nana, Hymenolepis diminuta)

PHYLUM: NEMATHELMINTHES

Family: Trichuridae (Trichocephalus trichuris)

Family: Capillaridae (Capillaria hepatica, Capillaria

philippinensis )

Family: Trichinellidae (Trichinella spiralis)

Family: Strongyloididae (Strongyloides stercoralis)

Family: Ancylostomidae (Ancylostoma duodenale,A. braziliense, A.

caninum, Necator americanus, Ternidens diminutus, Oesophagostomum

apiostomum)

Family: Ascarididae (Ascaris lumbricoides)

Family: Oxyuridae (Enterobius vermicularis)

Family: Filariidae (Wuchereria bancrofti, Dirofilaria immitis)

Family: Onchoceridae (Onchocerca volvulus)

10

6

12

36

PHYLUM: MOULLUSCA (SNAILS)

PROTOZOA

PHYLUM SARCOMASTIGOPHORA

CLASS: RHIZOPODA

Family: Endamoebidae

Entamoeba histolytica

Entamoeba hartmani

Entamoeba dispar

Entamoeba gingivalis

Endolimax nana

Iodamoeba butschlii

CLASS: ZOOMASTIGOPHORFA Family: Trypanosomatidae

Genus: Leishmania

Leishmania donovani complex

Leishmania tropica complex

Leishmania braziliensis compelx

Leishmania mexicana complex

Genus: Trypanosoma

Trypanosoma brucei

Trypanosoma brucei gaimbiens

Trypanosoma brucei rhodesiense

Trypanosoma cruzi

Trypanosoma rangeli

Trypanosoma lewisi

Family: Bodonidae: Genus: Retortamonas

Family: Tetramitidae Genus: Enteromonas

Family: Chilomastigidae Genus: Chilomastix

Family: Hexamitidae Genus: Giardia

Family: Monocercomonadidae Genus: Dientamoeba

Family: Trichomonadidae Genus: Trichomonas

PHYLUM CILIOPHORA Family: Balantidiidae

PHYLUM APICOMPLEXA (SPOROZOA)

SUBCLASS: COCCIDIA

SUBORDER: EIMERIINA

2 6

12 6 2 6

37

Family: Cryptosporidiidae (Cryptosporidium spp)

Family: Eimeriidae (Eimeria spp and Isospora spp)

Family: Sarcocystidae (Toxoplasma gondii and Sarcocystis spp)

SUBORDER: HAEMOSPORINA Family: Plasmodiidae

Plasmodium vivax

Plasmodium ovale

Plasmodium malariae

Plasmodium falciparum

Plasmodium berghie

SUBCLASS PIROPLASMEA Family: Babesiidae

MEDICAL ENTOMOLOGY

CLASS INSECTA

SUBCLASS: ENDOPTERYGOTE INSECTS (Holometabola) I. ORDER: DIPTERA [Mosquitoes and Flies]

A) SUBORDER: NEMATOCERA

Family: Psychodidae (Bloodsucking sandflies and non-biting moth-

flies)

Family: Culicidae

Genus: Anopheles

Genus: Culex

Genus: Aedes

Family: Simuliidae (Black flies, Buffalo gnat)

Family: Ceratopogonidae (Biting midges)

B) SUBORDER: BRACHYCERA

1. Infraorder: Tabanomorpha

Family: Tabanidae

Genus: Tabanus (Horse-flies - Myiasis)

Genus: Chrysops (Deer-flies)

2. Infraorder: Muscomorpha (Cyclorrapha)

a) Section: Aschiza

Erystalis spp

b) Section: Schizophora:

Family: Hypodermatidae (Hypoderma bovis))

Family: Glossinidae (Glossina spp)

Family: Muscidae (Musca domestica, Stomoxys

calcitrans)

8 2

10 4

38

Family: Fanniidae (Fannia spp)

Family: Oestridae (Oestrus ovis)

Family: Calliphoridae (Calliphora, Lucilia, Chrysomia)

Family: Sarcophagidae (Sarcophaga, Wohlfartia spp)

Family: Cuterebrinae (Dermatobia hominis)

II. ORDER: SIPHONEPTERA [Fleas]

Family: Pullicidae

Pulex irritans

Ctenocephalides canis

Ctenocephalides felis

Xenopsylla cheopis

Xenopsylla braziliensis

Xenopsylla astia

Family: Tungidae

Tunga penetrans

Nosopsyllus fasciatus

Leptopsylla segnis

SUBCLASS EXOPTERYGOTA (Hemimetabola)

I. ORDER : PHTHIRAPTERA SUBORDER: ANOPLURA (Lice: biting lice and sucking lice)

Family: Pediculidae : ( Pediculus humanus capitis,

Pediculus h. corporis

Family: Phthiridae ( Phthirus pubis)

II. ORDER: HEMIPTERA (Bugs, cicads, aphids, and scale insects)

SUBORDER: HETRROPTERA

Family: Cimicidae (Cimex lectularius)

Family: Reduviidae (Triatoma megista)

CLASS ARACHNIDA

SUBCLASS: ACARI

I. ORDER: PARASITIFORMES

SUBORDER: IXODIDA (Metastigmata: TICKS)

Family: Ixodidae (Hard Ticks)

Family: Argasidae (Soft Ticks)

II.ORDER: ACARIFORMES A) SUBORDER: ACARIDIDA (Astigmata)

Family: Sarcoptidae (Sarcoptes scabiei)

4 4 6

39

Family: Pyroglyphidae (House dust mites)

B) SUBORDER: ACTINEDIDA (Prostigmata)

FFaammiillyy: Demodicidae

Family: Trombiculidae

SUBCLASS ARANAE (SPIDERS)

SUBCLASS SCORPIONES (SCORPIONS)

CLASS CRUSTACEA

I. SUBCLASS COPEPODA ORDER: EUCOPEPODA (Cyclops)

II. SUBCLASS: MALACOSTRACA ORDER: DECAPODA (Cray fish, Lobster, Crabs)

2 2

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills.

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution:

Helminthology (1 paper)

Written: 240 Marks Practical: 120 Marks Oral: 40 Marks

Protozoology and Entomology (1 paper)

Written: 270 Marks Practical: 135 Marks Oral: 45 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books) a) Clinical Parasitology: by Craig, Faust , Beaver (Lippincott

Williams & Wilkins, US; 9th edition).

b) Manson's Tropical Diseases: by Cook and Zumla (Saunders Ltd.;

21st edition).

c) Foundations of Parasitology: by Schmidt and Roberts (Times Mirror

/ Mosby College Publishing).

d) Foundations of Parasitology: by Roberts and Janovy (McGraw-Hill

Higher Education; 7th

edition).

e) Human Parasitology: by Bogitsh, Carter, Oeltmann (Academic

Press 3rd

edition).

f) Medical Parasitology: A Practical Approach: by Gillespie, Hawkey

(Oxford University Press).

g) Modern Parasitology: by F.E.G. Cox (Blackwell Science, UK)

40

h) Parasitology and Vector Biology: by Marquardt, Demaree (Grieve

Academic Press; 2nd

edition).

i) Diagnostic Medical Parasitology: by Garcia (American Society for

Microbiology; 5th

edition).

j) Markell and Voge's Medical Parasitology: by John , Petri

(Saunders; 9th edition).

k) Atlas of Tropical Medicine and Parasitology: by Peters and Pasvol

(Mosby 6th

edition).

l) Immunology and Molecular Biology of Parasitic Infections: by

Warren (Blackwell Science Ltd; 3rd Ed edition).

m) Immunity to Parasites: How Parasitic Infections are Controlled: by

Wakelin (Cambridge University Press; 2nd

edition).

n) Biology of Parasitism: by David, Tschudi, Pearce (Kluwer

Academic Publishers, 2000).

5.3- Recommended Books

a) Diagnostic Medical Parasitology: by Garcia (American Society

for Microbiology; 5th

edition).

b) Manson's Tropical Diseases: by Cook and Zumla (Saunders

Ltd.; 21st edition).

5.4- Periodicals, Web Sites, etc

a) Egyptian Journal of Parasitology

b) Parasitologists United Journal

c) Egyptian Journal of Basic Sciences

d) Egyptian Journal of Immunology

e) American Journal of Tropical Medicine and Hygiene

f) Transaction of Royal Society of Tropical Medicine and Hygiene

g) Journal of Clinical Microbiology

h) Journal of Infectious Diseases

i) European Journal of Clinical Microbiology

j) Journal of Eukaryotic Microbiology

k) Parasitology Research

l) Parasite Immunology

m) Infection and Immunity

n) Journal of Immunology

o) International Parasitology

p) Journal of Parasitology

q) Clinical Microbial Review

r) Journal of Helminthology

s) Applied and Environmental Microbiology

t) WHO: www.who.int

41

u) Parasitology Division, Center of Disease Control:

www.cdc.dpdx.gov

v) Pubmed: www.ncbi.nlm.nih.gov

w) Clinical Microbial Review: www.cmr.asm.org

x) American Society of Microbiology: www.asm.org

y) TDR, a Special Programme for Research and Training in Tropical

Diseases: www.who.int/tdr/

z) The World Organisation for Animal Health (OIE):

http://www.oie.int/

Course Specification of Elective Courses

1. Course specification for Molecular Biology

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: Minor.

Department offering the program: Parasitology Department

Department offering the course: Biochemistry Department

Academic year / Level: First year/ second semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Molecular biology Code: E6002

Credit Hours: 1

Lecture: 1 hour/w (10w) Practical: 1 hour/w (10w) SDL

Total: 10 theoretical and 10 practical hours

Coordinator

Head of the department

B - Professional Information

1. Course Aims:

The course aims to provide a thorough understanding and practical experience

of molecular biology as it applies to infectious agents including:

a) Introducing the students to the basics of molecular biology and

biotechnology.

b) Training of the students on the different molecular biology techniques.

c) Application of such techniques in taxonomy, diagnosis, epidemiology and

chemotherapy of infectious organisms.

d) Equipping of the students with the specialized knowledge and skills

necessary for a research careers that demand knowledge of molecular

biology.

42

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Understand the structure, function and organization of microbial

genomes.

a2) Describe the main principles of methods for preparation and extraction

of DNA and RNA.

a3) Understand the main principles and methods of DNA amplification

(PCR, RT-PCR, Real-time PCR, etc.)

a4) Understand the principles and methods of DNA sequencing.

a5) Describe the main principles of DNA and RNA blotting

a6) Understand the concepts of recombinant DNA technology

a7) Understand the applications of molecular biology techniques in the

diagnosis, taxonomy, species and strain differention of

microorganisms.

.a8) Understand the applications of molecular biology techniques in

studying drug resistance, and vaccine and drug development.

a9) Understand the principle of proteomics and microarrays.

b) Intellectual skills

By the end of the course the candidate will be able to:

b1) Design primers from a known gene sequence.

b2) Differentiate between microorganism by RFLP and RAPD-PCR.

b3) Interpret the results of diagnostic molecular assays and discard false

results.

c) Professional and practical skills:

By the end of the course, students will be able to:

c1) Apply safety measures in the laboratory.

c2) Apply methods to prevent contamination of the lab and equipments.

c3) Follow proper method of disposal of biohazardous samples

(infectious, carcinogenic, etc.).

c4) Extract DNA and RNA.

c5) Perform PCR.

d) General and transferable skills:

By the end of the course the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

d3) Show willing and motivations for self learning and continuous

improvement.

d4) Represent data efficiently.

d5) Search gene and protein database.

d6) Use molecular biology software and vsit parasite genomes websites.

43

3- Course content:

Basic principles in molecular

biology

No. of hours

L (1h) T P SDL

1- Nucleic acid structure and

organization

3 √

2- Cell cycle, DNA replication and

repair

2

3- RNA transcription and

processing

2 √

4- Regulation of gene expression

2

5-The Genetic code, mutation and

protein synthesis

2

6- Recombinant DNA technology 2

7- Techniques of genetic analysis

-Extraction of DNA & RNA

- PCR & RT-PCR

- DNA electrophoresis

- Blotting techniques

- RFLP

- Microarray

6

8- Genetic variation &

DNA finger printing

2 2

13-Ethical, Legal, and Social

implications of genetic knowledge

2

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution

Written: 30 Marks Practical: 15 Marks Oral: 5 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Molecular Cloning: A Laboratory Manual (Third Edition) 2001:

Joseph Sambrook, David Russell (Eds.). Cold Spring Harbor

Laboratory Press.

44

b) PCR “The Basics” (Second Edition) 2006: M. J. McPherson and S. G.

Muller, Taylor and Francis.

c) A-Z of Quantitative PCR (IUL Biotechnology, No. 5) (IuL

Biotechnology Series) 2004: Stephen A., Bustin International

University.

d) Fundamental Molecular Biology by Lizabeth A. Allison

e) Essentials of Molecular Biology by George M. Malacinski.

f) Molecular Biology: Genes to Proteins by Burton E. Tropp.

g) Short Protocols in Molecular Biology (2 volume set) by Frederick M.

Ausubel, Roger Brent, Robert E. Kingston, and David D. Moore.

h) Basic Techniques in Molecular Biology (Springer Lab Manuals) by

Stefan Surzycki.

i) Molecular Biology of the Cell by Bruce Alberts, Alexander Johnson,

Julian Lewis, and Martin Raff.

j) Molecular Cell Biology by Harvey Lodish, Arnold Berk, Chris A.

Kaiser, and Monty Krieger.

5.3- Recommended Books

a) Molecular Cloning: A Laboratory Manual (Third Edition) 2001:

Joseph Sambrook, David Russell (Eds.). Cold Spring Harbor

Laboratory Press.

b) Molecular Biology of the Cell by Bruce Alberts, Alexander Johnson,

Julian Lewis, and Martin Raff.

c) Molecular Cell Biology by Harvey Lodish, Arnold Berk, Chris A.

Kaiser, and Monty Krieger.

5.4- Periodicals, Web Sites, etc

a) Pubmed: http://www.ncbi.nlm.nih.gov/

b) The Ensembl genome databases for vertebrates and other eukaryotic

species http://www.ensembl.org/index.html

c) Expert Protein Analysis System Proteomics server

http://www.expasy.org/

d) Bioinformatics search http://www.bioinformatik.de

e) DNA from the beginning: http://www.dnaftb.org/dnaftb/

f) Protein Data Bank http://www.rcsb.org

g) Phylogenetic Tree Prediction

http://www.genebee.msu.su/services/phtree_reduced.html

45

2. Course specification for Immunology

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: Minor.

Department offering the program: Parasitology Department

Department offering the course: Microbiology and Immunology Department

Academic year / Level: First year/ second semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Immunology Code: E6031

Credit Hours: 1

Lecture: 1 hour/w (10w) Practical: 1 hour/w (10w) SDL

Total: 10 theoretical and 10 practical hours

Coordinator

Head of the department

B - Professional Information

1. Course Aims:

The course aims to provide basic knowledge and understanding, and practical

experience of immunology to infectious agents including:

a) Basic knowledge and understanding of the immunity to microbes.

b) Training of the students on the immunologic assays used for the diagnosis

of microbial infection.

c) Training of the students on the techniques for assessing immune response

to pathogens.

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Define immunity, immune system and immune response.

a2) Know the types of immunity, components, phases and characteristics

of the adaptive immune response.

a3) Know the tissues and cells of the immune system.

a4) Know the nature of immunogen, haptens and adjuvants, and the

factors responsible for immunogenicity.

a5) Know the structure and function of the soluble mediators of immunity;

cytokines and antibodies.

a6) Understand the mechanisms and effector functions of cell mediated

immunity.

a7) Understand the mechanisms and effector functions of humoral

immune response.

46

a8) Understand the immunological mechanisms underlying:

- Immune regulation and immunologic tolerance,

- Inflammation,

- Protection against microbial disease,

- Hypersensitivity or allergic reactions,

- Congenital and acquired immunodeficiencies.

a9) Understand the basics of antigen-antibody reactions and their

applications in diagnosis of infections.

b) Intellectual skills

By the end of the course the candidate should be able to:

b1) Differentiate between the two types of immunity.

b2) Differentiate between the two limbs of the adaptive immunity.

b3) Predict the type of the immune response to intracellular and

extracellular pathogens.

b4) Interpret immunological reports.

b5) Correlate the immune response and pathogenecity.

c) Professional and practical skills:

By the end of the course the candidate will be able to:

c1) Apply safety measures in the laboratory.

c2) Perform and interpret antigen-antibody assays as agglutination,

precipitation, enzyme immunoassays, immunofluorescence and

immunoblotting.

d) General and transferable skills:

By the end of the course the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

d3) Show willing and motivations for self learning and continuous

improvement.

d4) Represent data efficiently.

4- Course content:

Topics No of hours

Lecture Practical

Humoral and cellular immune response 2

Cytokines as modulators of immune response

Type 1 and Type 2 helper cells

2

Immunity to microbes 2

Hypersensitivity reactions 2

Immunodeficiency 2

Antigen-antibody reactions 8

In vitro assessment of cellular function 2

47

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills.

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution

Written: 30 Marks Practical: 15 Marks Oral: 5 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Basic Immunology by Abul K. Abbas and Andrew H. Lichtman (3rd

edition).

b) Roitt's Essential Immunology by Peter Delves, Seamus Martin,

Dennis Burton, and Ivan Roitt.

c) Immunology: A Short Course by Richard Coico and Geoffrey

Sunshine.

d) Cellular and Molecular Immunology: by Abul K. Abbas, Andrew

H. Lichtman (2003) (5th

edition).

e) Immunology for Medical Students by Roderick Nairn PhD and

Matthew Helbert.

f) Immunobiology, The Immune System in Health and Disease

(2005), 6th Edition Charles A. Janeway, Paul Travers, Mark

Walport and Marl J. Shlomchik Churchill Livingstone, New York.

5.3- Recommended Books

a) Basic Immunology by Abul K. Abbas and Andrew H. Lichtman (3rd

edition).

b) Cellular and Molecular Immunology: by Abul K. Abbas, Andrew

H. Lichtman (2003) (5th

edition).

c) Immunology, (2006), 7th Edition, David Male, Jonathan Brostoff,

David B Roth, Ivan Roitt. Mosby, London.

5.4- Periodicals, Web Sites, etc

a) Egyptian Journal of Immunology

b) Parasite Immunology

c) Infection and Immunity

d) Journal of Immunology

e) Pubmed: www.ncbi.nlm.nih.gov

f) Microbiology, Immunology and Parasitology course online “South

Carolina University: www.med.sc.edu:85/book/parasit-sta.htm

48

3. Course specification for Vector Biology

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: Minor

Department offering the program: Parasitology Department

Departments offering the course:

Parasitology Department Faculty of Medicine, Ain Shams University

Academic year / Level: First year/ second semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Vector biology Code: E6032

Credit Hours: 1

Lecture: 1 hour/w (10w) Practical: 1 hour/w (10w) SDL

Total: 10 theoretical and 10 practical hours

Coordinator

Head of the department

B - Professional Information

1. Course Aims:

The course aims to provide basic knowledge and understanding of:

a) Vector behaviour and ecology and vector-parasite interactions.

b) How these features impact on the epidemiology and control of vector-

borne diseases.

c) The importance of accurate vector identification in surveillance and

control.

d) Appropriate sampling methods for the major groups of vectors.

e) Practical entomological techniques used in vector identification and

incrimination, and in the study of vector competence, behaviour and

ecology.

f) Control of vectors.

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Realize the importance of arthropods in causing and transmitting

microbial infections.

a2) Understand vector bionomics including:

- Breeding places and habits,

- Feeding habits, biting behaviour and adaptation of mouth parts to

the feeding habits, blood and host preference (specificity),

- Flight range,

- Swarming, mating behaviour, and oviposition,

49

- Hibernation and overwintering.

a3) Understand the life cycle and development of arthropods.

a4) Know the modes of infections and transmission of pathogens by

arthropods.

a5) Describe the life cycle of the parasite inside their vector.

a6) Understand parasite vector interaction.

a7) Explain the disease caused by arthropods.

a8) Understand the relation between arthropods and allergy.

a9) Understand the vector immunity to pathogens.

a10) Understand the methods of vector control; physical, chemical,

biological and genetic control.

a11) Describe the side effects of insecticides.

a12) Understand the methods of vector trapping (light traps, sticky traps,

tsetse traps).

a13) Understand the methods of detection of pathogens inside arthropods

(incrimination techniques) as dissection of infected vectors, ELISA

and PCR.

b) Intellectual skills

By the end of the course the candidate should be able to:

b1) Correlate the bionomics with methods of control.

b2) Correlate the distribution of diseases with geographical distribution of

their vectors.

b3) Differentiate between the modes of transmission of pathogens by

arthropods.

c) Professional and practical skills:

By the end of the course the candidate will be able to:

c1) Indentify arthropod through morphological keys.

c2) Collect (trap) vectors from their breeding places.

c3) Apply the required precaution to prevent self infection while

collecting vectors.

c4) Isolate house dust mites from dust samples

c5) Dissect arthropods to detect pathogens.

c6) Prepare antigen from arthropods and use them in immunological

assays and skin testing.

c7) Control of arthropods by using suitable physical, chemical and

biological methods.

d) General and transferable skills:

By the end of the course the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

50

d3) Show willing and motivations for self learning and continuous

improvement.

d4) Represent data efficiently.

3- Course contents:

Topics No of hours

Lecture Practical SDL

Role of arthropods in disease

transmission

2

Vector bionomics 4

Parasite vector interaction 2

Vector control 2

Vector identification keys 2

Collection of vectors 2

Isolation of mites from house dust 2

Preparation of antigens from

arthropods

4

Methods of detection of

pathogens inside vectors

√

Vector immunity to pathogens √

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills.

4.2 Practical exams to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution

Written: 30 Marks Practical: 15 Marks Oral: 5 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Clinical Parasitology: by Craig, Faust , Beaver (Lippincott Williams

& Wilkins, US; 9th edition).

b) Manson's Tropical Diseases: by Cook and Zumla (Saunders Ltd.; 21st

edition).

c) Foundations of Parasitology: by Schmidt and Roberts (Times Mirror /

Mosby College Publishing).

51

d) Foundations of Parasitology: by Roberts and Janovy (McGraw-Hill

Higher Education; 7th

edition).

e) Human Parasitology: by Bogitsh, Carter, Oeltmann

f) Parasitology and Vector Biology: by Marquardt, Demaree (Grieve

Academic Press; 2 edition).

g) Biology of Parasitism: by David, Tschudi, Pearce (Kluwer

Academic Publishers, 2000).

5.3- Recommended Books

a) Parasitology and Vector Biology: by Marquardt, Demaree (Grieve

Academic Press; 2 edition).

b) Manson's Tropical Diseases: by Cook and Zumla (Saunders Ltd.;

21st edition).

5.4- Periodicals, Web Sites, etc

a) Egyptian Journal of Parasitology

b) American Journal of Tropical Medicine and Hygiene

c) Transaction of Royal Society of Tropical Medicine and Hygiene

d) WHO: www.who.int

e) Parasitology Division, Center of Disease Control: www.cdc.dpdx.gov

f) Pubmed: www.ncbi.nlm.nih.gov

g) London School of Hygiene and Tropical Medicine “Intensive Course

in Vector Biology” www.lshtm.ac.uk/dcvbu/vbi.htm

h) TDR, a Special Programme for Research and Training in Tropical

Diseases: www.who.int/tdr/

i) The World Organisation for Animal Health (OIE):

http://www.oie.int/

52

4. Course specification for Biostatistics

University: Ain Shams Faculty: Medicine

Program(s) on which the course is given: Master of Science in Parasitology

Major or minor element of programs: Minor

Department offering the program: Parasitology Department

Departments offering the course: Community, Environmental and Occupational

Medicine

Academic year / Level: First year/ second semester

Date of specification approval ……………………………………..

A- Basic Information

Title: Biostatistics Code: E6009

Credit Hours: 1

Lecture: 1 hour/w (10w) Practical: 1 hour/w (10w) SDL

Total: 10 theoretical and 10 practical hours

Coordinator

Head of the department

B - Professional Information

1. Course Aims:

To enable the candidate to plan, conduct, analyze and interpret the results of a

research in his specific field. This course unit introduces the application of

statistical ideas and methodology to medical research.

2. Intended learning outcomes (ILOs):

a) Knowledge and Understanding

By the end of the course the candidate will be able to:

a1) Know the definition and types of variables.

a2) Understand the basics of descriptive statistics as mean, median,

standard deviation and standard error.

a3) Know the concept of normal distribution and confidence of interval and

hypothesis testing.

a4) Understand the basics of probability and significance tests.

a5) Understand the basic of analysis of categorical data, comparing of

means and proportions and analysis of variance (ANOVA).

a6) Understand the concepts of correlation and regression

a7) Understand the applications of transformation and non-parametric

methods.

a8) Know sampling methods, calculation of required sample size and

sources of statistical bias and errors.

a9) Know the computer software used for statistical calculation.

b) Intellectual skills

By the end of the course the candidate will be able to:

53

b1) Interpret the results of the statistical techniques.

b2) Select the appropriate statistical analysis test for a given data.

b3) Select the appropriate non-parametric when required.

b4) Detect sources of confounding and errors.

c) Professional and practical skills:

By the end of the cours, the candidate will be able to:

c1) Calculate frequency, mean, median, standard deviation and standard

error.

c2) Analyze a single mean (z-test).

c3) Compare means and proportion using parametric tests (t, f, ANOVA,

Chi-squared tests) and their non-parametric equivalents.

c4) Interpret the significance of the above test.

c5) Determine the relationship between different variables using

correlation coefficient and linear regression.

c4) Represent data by tabulation and suitable diagrams as bar, pie, and

linear charts.

c5) Use statistical software as SPSS an EPI-INFO, EXCEL, STATA, etc.

d) General and transferable skills:

By the end of the course the candidate will be able to:

d1) Show respect to colleagues and senior staff.

d2) Cooperate with colleagues, administrative staff and work in team.

d3) Show willing and motivations for self learning and continuous

improvement.

3- Course contents:

Topics No of hours

Lecture Practical

Introduction to medical statistics and its

uses

1

- Types of variables and Descriptive

statistics

- Calculation of mean, median, standard

deviation and standard error.

1

2

Graphic presentation of data 2

Introduction to probability theory and

rules

1

Confidence interval of means and

proportions

1

Comparing of means, proportions and

analysis of variance (t, f, z tests, Anova)

2 3

Correlation and regression 2

54

Non-parametric tests 1

Sampling methods and sample size 1

Use statistical software as SPSS an EPI-

INFO, EXCEL, STATA, etc.

3

4 - Student Assessment Methods

4.1 Written exams (Essay, MCQ, interpretation) to assess knowledge,

understanding and intellectual skills

4.2 Practical exercises to assess professional and intellectual skills.

4.3 Oral exams to assess knowledge, understanding, intellectual and general

transferable skills.

Marks distribution

Written: 30 Marks Practical: 15 Marks Oral: 5 Marks

5- List of References

5.1- Course Notes (paper and / or electronic)

5.2- Essential Books (Text Books)

a) Statistics in Clinical Practice. avid Coggon. BMJ Books. 2nd

edition

2003

b) Basic and Clinical Biostatistics (Lange Basic Science) by Beth

Dawson, Robert Trapp, Beth Dawson, and Robert Trapp

c) Epidemiology, Biostatistics and Preventive Medicine by James F.

Jekel, David L. Katz and Joann G. Elmore

5.3- Recommended Books

Handbook of Epidemiology. Springer 2005

5.4- Periodicals, Web Sites, etc

a) http://www.brettscaife.net/statistics/introstat/

onlinestatbook.com/rvls/

b) http://www. Epidemiolog.net

c) http://www.shef.ac.uk/scharr/spss/

55

V- General Information

1 – Monitoring Of Training and Submission of Training Reports

You must keep proper and updated records in your logbook to reflect the

activities encountered in your training. Your logbook must be duly endorsed by an

authorized signatory at the end of each semester.

You will be continuously assessed by your supervisors, in consultation

with head of department. An assessment will be submitted within 2 weeks of

completion of each semester.

2 - Miscellaneous Information:

Injury and/or Blood or Body Fluid Exposure:

During regular working hours, you should immediately report an exposure incident

to Head of the Department. If exposure occurs after regular working hours or

during a weekend or holiday; please call the ----------------------------------------. For

injury, please report to the Emergency Department.

Please also be sure to inform the supervisors of an exposure incident and/or

injury.

3 - Action Completion of Clinical Training

Once all training sessions are completed the log book should be signed by the senior

supervisor and the head of the department and then should be submitted to post

graduate Secretariat.

4- Reference

The Training Guide is available at the post graduate Secretariat and could be

downloaded from the following website is

---------------------------------------------------------------

VI – Your log book

1- Introduction

The aim of this book is to give you a guide to the expectations for each item. It will

be a guide for both you and your teachers to what you should be seeing and doing.

It will give you a list of the important topics that you should think about and should

be covered in:

1. Laboratory

2. Seminars

56

3. Tutorials

4. Self-directed learning (SDL)

Remember

This document is only a guide. It is not an exhaustive list. It is not just a checklist

to score points. It is a guide to encourage you to read and learn more. This book is

for your benefit. It will form a record of your clinical training and experience.

2 - Supervisors

1. Prof. Dr. Head of the department

2. Prof. Dr.

3. Prof. Dr.

4. Prof. Dr.

5. Prof. Dr.

3–Tables for Training Records

The candidate will record the details and date of each activity, and the authorized

staff member signature.

Candidates are required to fulfill 75% of the listed activities in order to be eligible

for the exam entry. The minimum number required for each activity = 75%. You

are free to attend more and record your extra attendance.

Weekly Department Plan

Day /time Saturday Sunday Monday Tuesday Wednesday Thursday

Theoretical 10-13

Practical 8-12

Seminar 10-12

Working in

Parasitology

Diagnostic

unit

9-13 9-13 9-13 9-13 9-13 9-13

Monthly activity: Thesis attendance

Yearly activity: Annual Conferences

57

Seminars Attendance

(NB. Minimum number required is 5)

No. Date Place Topic Supervisor

Signature

1

2

3

4

5

The student should submit a report on the seminar.

Conferences Attendance

(NB. Minimum number required is 2)

No. Date Place Topic Supervisor

Signature

1

2

The student should submit a certificate of attendance or participation.

58

Thesis Attendance

(NB. Minimum number required is10)

No Date Place Title of the thesis Supervisor

signature

1

2

3

4

5

6

7

8

9

10

The student should submit a report on the thesis discussion.

59

Update Articles Presentation

(NB. Minimum number required is 2)

No. Date Place Topic Supervisor

Signature

1

2

Stool Examination for Parasites by Direct Smear Method

(NB. Minimum number required 100*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

6

7

8

9

10

11

60

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

61

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

49

50

51

62

52

53

54

55

56

57

58

59

50

61

62

63

64

65

66

67

68

69

70

71

63

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

90

91

64

92

93

94

95

96

97

98

100

At least 50 with score A.

Urine Examination for Parasites

(NB. Minimum number required 50*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

6

7

8

65

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

66

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

67

49

50

At least 25 with score A.

Stool Concentration Techniques

(NB. Minimum number required is 20*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

68

16

17

18

19

20

At least 10 with score A.

Cultivation of the Larval Stages of Nematodes

(NB. Minimum number required is 5*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

At least 3 with score A.

69

Blood Examination for Parasites

(NB. Minimum number required is 15*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

At least 8 with score A.

70

Staining of Stool Specimens for Detection of Parasites

(NB. Minimum number required is 10*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

6

7

8

9

10

At least 5 with score A.

71

Serodiagnosis of Parasitic Infections

(NB. Minimum number required is 5*)

No. Date Semester Place Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

At least 3 with score A.

Preparation of Preserved stool samples

(NB. Minimum number required is 5*)

No. Date Semester Specimen Score

(A, B, C, D)

Points Supervisor

Signature

1

2

3

4

5

At least 3 with score A.

72

- Activities should be distributed over the 4 semesters.

- Activities will be evaluated by the supervisors using check lists.

- Activities will be scored and graded according to the proficiency of

performance into:

1. A: Excellent performance: 85-100 % of the score

2. B: Very Good performance: 75-84 % of the score

3. C: Good performance: 65-75 % of the score

4. D: Unacceptable and should be repeated

73

4 - Log book preview

The candidate logbook will be reviewed and patients seen/ skills performed

summarized by diagnosis groups during the semester evaluation and at the end of

the course in the table below. This reflects the number of activity done by

category. (According to each degree) The results of this review will be totaled in

the summary chart below.

Summary

Semester 1st 2

nd 3

rd 4

th Total

Activity No No No No

Seminars attendance (5)

Conferences attendance (2)

Thesis discussion attendance (10)

Update article presentation (2)

Stool examination for parasites (100)

Urine examination for parasites (50)

Stool Concentration techniques (20)

Cultivation of the Larval Stages of

Nematodes (5)

Blood examination for parasites (25)

Staining of stool specimens for

detection of parasites (10)

Serodiagnosis of parasitic infections (5)

Preparation of preserved stool samples

Supervisor signature

VII - Head of Department Approval for the Exam Entry

__________________________________________________________________

____________________________________________

__________________________________________________________________

____________________________________________

74

VIII – Thesis Follow up

التقزيز النصف السنىي لعام / التاريخ/ عن الطالب /

الوقيد لدرجة ) هاجسيتز ( بقسن /

تقزيز السادة االساتذة الوشزفين

بدأ جوع الوادة العلوية و كتابة الوقدهة

قطع شىطا هحدودا

أوشك عل اإلنتهاء

وعإنته هن الج

بدأ الجزء العول

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن التحليل

بدأ هناقشة النتائج

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن التحزيز

بدأ الوزاجعة النهائية هع الوشزف

قطع شىطا هحدودا

شك عل اإلنتهاءأو

إنتهت تواها

إستوزار قيد الطالب رأي السادة الوشزفين

هد القيد

شطب قيد الطالب

نعن تن تشكيل لجنة الوناقشة

ال تاريخ التشكيل

/ /

/ شطب القيد هد

وكيل الكلية تىقيع السادة الوشزفين

75

76

التقزيز النصف السنىي لعام / التاريخ/ عن الطالب /

الوقيد لدرجة ) هاجسيتز ( بقسن /

لوشزفينتقزيز السادة االساتذة ا

بدأ جوع الوادة العلوية و كتابة الوقدهة

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن الجوع

بدأ الجزء العول

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن التحليل

بدأ هناقشة النتائج

قطع شىطا هحدودا

وشك عل اإلنتهاءأ

إنته هن التحزيز

بدأ الوزاجعة النهائية هع الوشزف

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنتهت تواها

إستوزار قيد الطالب رأي السادة الوشزفين

هد القيد

شطب قيد الطالب

نعن ة الوناقشة تن تشكيل لجن

ال تاريخ التشكيل

/ /

هد / شطب القيد

وكيل الكلية تىقيع السادة الوشزفين

77

78

التاريخ/ التقزيز النصف السنىي لعام / عن الطالب /

الوقيد لدرجة ) هاجسيتز ( بقسن /

تقزيز السادة االساتذة الوشزفين

بدأ جوع الوادة العلوية و كتابة الوقدهة

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن الجوع

بدأ الجزء العول

هحدوداقطع شىطا

أوشك عل اإلنتهاء

إنته هن التحليل

بدأ هناقشة النتائج

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن التحزيز

بدأ الوزاجعة النهائية هع الوشزف

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنتهت تواها

إستوزار قيد الطالب ة الوشزفينرأي الساد

هد القيد

شطب قيد الطالب

نعن تن تشكيل لجنة الوناقشة

ال تاريخ التشكيل

/ /

هد / شطب القيد

كيل الكليةو تىقيع السادة الوشزفين

79

80

التقزيز النصف السنىي لعام / التاريخ/ عن الطالب /

الوقيد لدرجة ) هاجسيتز ( بقسن /

تقزيز السادة االساتذة الوشزفين

بدأ ةجوع الوادة العلوية و كتابة الوقده

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن الجوع

بدأ الجزء العول

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن التحليل

بدأ هناقشة النتائج

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنته هن التحزيز

بدأ الوزاجعة النهائية هع الوشزف

قطع شىطا هحدودا

أوشك عل اإلنتهاء

إنتهت تواها

إستوزار قيد الطالب رأي السادة الوشزفين

هد القيد

شطب قيد الطالب

نعن تن تشكيل لجنة الوناقشة

ال تاريخ التشكيل

/ /

هد / شطب القيد

وكيل الكلية تىقيع السادة الوشزفين

81

82

VIII – Evaluation Forms

To be completed at -------------------------------------

Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Agreed educational objectives and timescale in which objectives should be

achieved.

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Candidate

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Supervisor

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Date of next meeting

Signed by candidate

Signed by Supervisor

Date

83

84

VIII – Evaluation Forms

To be completed at -------------------------------------

Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Agreed educational objectives and timescale in which objectives should be

achieved.

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Candidate

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Supervisor

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Date of next meeting

Signed by candidate

Signed by Supervisor

Date

85

86

VIII – Evaluation Forms

To be completed at -------------------------------------

Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Agreed educational objectives and timescale in which objectives should be

achieved.

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Candidate

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Supervisor

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Date of next meeting

Signed by candidate

Signed by Supervisor

Date

87

88

VIII – Evaluation Forms

To be completed at -------------------------------------

Candidate

Supervisor

Location

Aim of training

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Agreed educational objectives and timescale in which objectives should be

achieved.

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Candidate

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Comments by Supervisor

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

__________________________________________________________________

Date of next meeting

Signed by candidate

Signed by Supervisor

Date

89

90

Degree Program Evaluation Form by The Candidate

To be completed at the end of your degree.

Please consider each pair of statements and decide which most clearly reflects your

view and tick one box or answer the question.

I. Individual Information

1. Are you a graduate of ASU?

yes no to some degree

2. Year and semester when studies began:

II. General Questions

1. What are the advantages/disadvantages of the general study environment at the

University ASU?

2. What were your expectations when you applied to the degree?

3. Do you feel that the degree program prepares you well for your future studies or

employment according to the demands and expectations of those institutions?

yes no to some degree

4. Has the time limit of the program (two or three academic years) caused you any

difficulties or inconveniences?

yes no to some degree

III. Structure of Degree Program

1. Did you receive enough guidance in planning your study schedule in the

beginning of the program?

yes no to some degree

2. What were the main difficulties in the planning of your study schedule?

3. What is your general opinion on the structure of the degree program?

91

4. In your opinion, does the degree program offer a good balance of lectures,

seminars, conferences, and book exams?

yes no to some degree

a) General Studies

i) Do you feel that you have received enough guidance on academic writing?

yes no to some degree

ii) Do you feel that you have acquired sufficient knowledge on research skills (eg.

quantitative and qualitative research methods)?

yes no to some degree

b) Courses

i) Have you had some special difficulties in completing some of the courses?

Please specify.

ii) Has there been a sufficient variety of courses offered for your optional studies?

yes no to some degree

iii) Have you received enough guidance for the preparation of your thesis?

yes no to some degree

IV. Concluding Points

1. Did the degree program meet your expectations?

yes no to some degree

2. What aspects of the degree program do you particularly like?

3. What aspects of the degree program do you particularly dislike?

4. What are your suggestions on how to improve the program?

Thank you!