INSTITUTIONEN FÖ R BIOLOGI OCH MILJÖ VETENSKAP

Molecular Systematics of Limnodrilus

(Annelida: Clitellata)

Yingkui Liu

Institutionen för biologi och miljövetenskap

Naturvetenskapliga fakulteten

Akademisk avhandling för filosofie doktorsexamen i naturvetenskap inriktning biologi, som

med tillstånd från Naturvetenskapliga fakulteten kommer att offentligt försvaras måndag den

20, mars, 2017 kl. 13:00 i Föreläsningssalen, institutionen för biologi och miljövetenskap,

Medicinaregatan 18A (Zoologen), 413 90 Göteborg.

ISBN: 978-91-629-0111-0 (PDF)

ISBN: 978-91-629-0112-7 (Print)

Tillgänglig via http://hdl.handle.net/2077/51417

Molecular Systematics of Limnodrilus (Annelida: Clitellata) Yingkui Liu

Abstract

The freshwater Limnodrilus worms (Clitellata: Naididae: Tubificinae) are segmented hermaphroditic annelids, bearing a unique clitellum (“girdle”) during sexual maturity. Some abundant and common taxa of Limnodrilus are ecologically and economically important in many respects, but taxonomic controversy, especially regarding the diagnosis of the cosmopolitan Limnodrilus hoffmeisteri Claparède, 1862(the type species of the genus), has lasted for more than a century. In addition, the phylogenetic position of Limnodrilus within the subfamily Tubificinae has been uncertain.

Taxonomic studies based on molecular data, e.g., using DNA-barcoding (for animals, the mitochondrial marker COI), have revealed several examples of cryptic speciation among widely distributed clitellate morphospecies. In this thesis, I used both mitochondrial COI barcodes and nuclear ITS data to explore primary species hypotheses from a sample of the morphologically defined L. hoffmeisteri collected in the northern hemisphere, and a final conclusion about species boundaries was based on the congruence of the mitochondrial and nuclear phylogenies. Furthermore, the phylogeny of Limnodrilus was estimated based on multiple-loci data of severalLimnodrilus species, including a new one described as L. sulphurensis Fend, Liu & Erséus, 2016 from a sulphur cave in North America, and other naidid taxa. Finally, as the commonly used ITS primers are neither efficient nor specific for clitellates, two new pairs of clitellate-specific ITS primers were proposed.

The molecular study showed that the well-known taxon “L. hoffmeisteri”actually represents a species complex (with at least ten species) rather than a single, cosmopolitan, species with great morphological variation. This work also showed that DNA barcoding, without using additional nuclear data, is likely to overestimate the number of species. Therefore, the ITS primers specific for clitellates will facilitate future research on species delimitation and the evaluation of mitochondrial DNA barcoding in Clitellata as a whole. In addition, by combining morphological and genetic information, a neotype of L. hoffmeisteri sensu stricto was designated, and the new species L. sulphurensis was discriminated from the other members of this genus. The neotype of L. hoffmeisteri is a baseline for future taxonomic work on the many cryptic species.

The phylogenetic hypothesis presented in this thesis contributes to our understanding of Limnodrilus sensu stricto, which is a well-demarcated, monophyletic genus of the naidid subfamily Tubificinae, containing at least three main evolutionary lineages (i.e., three species groups). The sister lineage of Limnodrilus in our taxon sample is a group of three genera, Baltidrilus, Lophochaeta and Varichaetadrilus. However, Limnodrilus rubripenis Loden, 1977 is phylogenetically closer toVarichaetadrilus than to other Limnodrilus species.

Keywords: Cryptic diversity, Species delimitation, Oligochaeta, Clitellata, Sludge worms, Limnodrilus, Integrative taxonomy, Neotype, DNA-barcoding, Phylogeny, Primers.

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