(mm) mm Cat.No.
0.1 2000 5020-10018 580,000
(mm) mm Cat.No.
0.1 2000 5020-10015 530,000
MonoCap C18 High Resolution 750
(mm) (mm) Cat.No.
0.05
750
2 μm 15 nm 0.1 mm I.D. 2000 mm 0.3 μL/min 1 μL/min 35 MPa
2 μm 11 nm 0.1 mm I.D. 2000 mm 0.3 μL/min 1 μL/min 35 MPa
2 μm 15 nm 0.2 mm I.D. 750 mm 1.0 μL/min 3 μL/min 22 MPa
30
HPLC
MonoCap C18 High Resolution
4
40 80 120 160 200 240 280 320 360 400 440 480 520 560 6000
Conditions System Column
Trap column Eluent
10,00020,000
: GLS Capillary HPLC system : MonoCap C18 High Resolution 2000
(2000 mm × 0.1 mm I.D) : Particle packed column (3 μm, 150 mm ×
0.075 mm I.D.) : MonoCap C18 Trap Column (50 mm × 0.075 mm I.D.) :
A) 0.1 %HCOOH in CH3CN
B) 0.1 %HCOOH in H2O A/B = 10/90 - 600 min - 45/55 A/B = 10/90 -
180 min - 45/55
Time(min)
Time(min)
: 0.5 μL/min 0.3 μL/min
: 5 μL : MS (TIC m/z 500 - 1500) : Tryptic digest of proteins
Flow Rate
Conditions Column
MonoCap C18 High Resolution 2000
MonoCap C18 High Resolution 2000 (2000 × 0.1 mm I.D.) MonoCap C18
High Resolution Ultra 2000 (2000 × 0.1 mm I.D.) (3 μm, 150 × 0.075
mm I.D.)
A) 0.1 % HCOOH in CH3CN B) 0.1 % HCOOH in H2O A/B = 10/90,v/v
25 .
MonoCap C18 High Resolutin Ultra 2000 2000 × 0.1 mm I.D 2339
MonoCap C18 High Resolutin 2000 2000 × 0.1 mm I.D 2087
150 × 0.1 mm I.D. 680
LC/MS/MS
MonoCap C18 High Resolution
THP-1Lysate (5 μg) MonoCap C18 High Resolution Ultra 2000 10
MonoCap C18 High Resolution 2000 10 Particle packed column; 2
μL/min
2 μm 12 nm 0.1 mm I.D. 2000 mm 0.3 μL/min-1 μL/min : 35 MPa
H
=O
-CH2CH2CH2-N-C-NH2
MonoCap® HILIC-UP High Resolution 2000
(mm) mm Cat.No.
0.1 2000 5020-10019 580,000
7194 2201
In te
ns ity
: MonoCap HILIC-UP High Resolution 2000 : A) CH3CN : H2O=10/90
(0.5% CH3COOH)
B) CH3CN : H2O=95/5 (0.5% CH3COOH) A/B=0/100-(240 min)-20/80-(10
min)-100/0 -(10min)-100/0,v/v
: 500 nL/min : 1 μL : TIC MS(m/z 300-1500)
4000 55006500HILIC-UP C18
HILIC-UPC18
Column size : 2000 × 0.1 mm I.D., Mass spectrometer: Triple TOF
5600 (482
C18HILIC-UP 4
PEEK
MonoCap C18 Fast-flow
0 2 4 6 8 10
0
PEEK
2 PEEK 50 mm 3
2 PEEK 50 mm 3